Best3 (NM_001007583) Mouse Tagged ORF Clone
CAT#: MG220298
- TrueORF®
Best3 (tGFP-tagged) - Mouse bestrophin 3 (Best3), (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001007583" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Best3 |
Synonyms | mBest4; Vmd2l3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG220298 representing NM_001007583
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGTCACTTACTCCAGTAAAGTAGCAAATGCAACATTTTTTGGATTTCATAGGTTGCTTCTCAAGT GGAGAGGTAGTATCTACAAGCTGCTGTACAGGGAATTTATTGTTTTTGCTGTTCTTTACACAGCAATCAG TCTGGTGTACAGATTGTTACTTACAGGAGCCCAAAAACGTTACTTTGAAAAATTATCAATTTACTGTGAC AGATATGCTGAACAAATTCCAGTAACCTTTGTGCTTGGGTTTTACGTCACTCTGGTAGTGAACCGATGGT GGAACCAGTTTGTGAATCTGCCTTGGCCAGACAGGCTGATGCTCCTCATTTCCAGCAGTGTCCACGGGAG CGACCAGCATGGGCGCCTGCTCAGAAGGACGCTGATGCGCTACGTCAACCTGACGTCCCTGCTCATCTTC CGCTCGGTGAGCACCGCGGTGTACAAGAGGTTTCCCACCATGGACCACGTGGTCGAAGCAGGTTTTATGA CGGCAGATGAGAGGAAATTATTCGACCACCTCAAATCGCCTCATCTGAAGTACTGGGTTCCCTTCATTTG GTTTGGAAATCTTGCAACCAAAGCCCGGAATGAAGGCAGAATCAGAGACAGCGTTGATCTACAATCACTG ATGACTGAAATGAATCGGTACCGCTCTTGGTGCAGCCTCTTATTTGGTTACGACTGGGTTGGCATTCCGC TGGTTTACACACAGGTCGTCACCCTCGCCGTCTACACGTTCTTCTTTGCGTGCCTGATCGGAAGGCAGTT CCTGGATCCCACCAAAGGCTACGTAGGACATGACTTGGATCTGTACGTTCCCATCTTCACTCTCCTCCAG TTCTTCTTCTACGCAGGATGGCTCAAGGTTGCAGAGCAGCTGATCAACCCTTTCGGGGAAGATGACGATG ACTTTGAAACAAACTGGTGCATTGACAGAAACCTGCAGGTCTCTCTCTTGGCTGTGGATGAAATGCATAT GAGTTTGCCCAAGATGAAGAAGGACATTTACTGGGACGATTCTGCTGCCCGGCCACCGTACACACTGGCG GCTGCCGACTACTGCATACCGTCCTTTCTGGGCTCAACGATCCAAATGGGGCTGTCCGGGTCCAACTTCC CCGCAGAGGACTGGCTGTGGAACTACGAGAAACACGGAAACCGGCACTCAGTGATGAGGAGGGTCAAGCG CTTCCTGAGTACCCACGAGCATCCTGGCAGCCCCAGGAGGAGGAGGAGCTTCGGCAGGCAGGCCAGCGAC AGCTCCATGTTCTTACCCCCAAGCCCAGCTCGGGATCTGCTGGATGTGCCGTCCAGGAACCCCCACCGAG GCTCGCCCACACGGAAGCAATCCCGCTCCCAAGAGGGCAGCCCCAAGCTGCACTCCAGCATGGGAGAACT GTCCACCATCAGGGAGACCAGCCGTACCAGCACGCTGCAGAGCCTGAGCCCGCAGTCCAGCGTGAGGTCC TCCCCTACCAAAATGCCCCAGGTCCCTGAGGTCCTAATCACCGCCGCTGAAGCACCAGCGTTCTCAGCAG ACAGCCATCAGCATGACTCCACTACCTCCATCTTGAGCCTCGAGTTCACCGGGGTGCAGCCGAGCGGGAC TGAGCAGCAGGTAGAGCCTTCAGGGACCCCACCTGGAGACCCCAATCCCCAGACTACTTCAGCCAGTACT GAAAGAGACTTGTTCAAGTTCGAGGAAGATCTAGAGGATGACAGATTTCCCAAAAGGTGGAGCCTGCCAG AGTTTCTGGAGTCCAGGCACACCTCGCTGGGAAACTTAGGTCCAGATCCTGTGAGTCCCCGGGATGCTCT TCTTTTACCTGACACAGAAACACCCTCAGAGACCAACGGAATCCATCCTGGGGCTGGCTCTGCCCTCGCC CCCGACATCCTGTACTTAATGGAAAGTTTGGATAAGGAAACAGACATCTTGGAGTTTAACAACGAGCACA CTGGAGAATCCCCGAAGGGAACACCACAGCGCCCCAGGACCTGGTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG220298 representing NM_001007583
Red=Cloning site Green=Tags(s) MTVTYSSKVANATFFGFHRLLLKWRGSIYKLLYREFIVFAVLYTAISLVYRLLLTGAQKRYFEKLSIYCD RYAEQIPVTFVLGFYVTLVVNRWWNQFVNLPWPDRLMLLISSSVHGSDQHGRLLRRTLMRYVNLTSLLIF RSVSTAVYKRFPTMDHVVEAGFMTADERKLFDHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSL MTEMNRYRSWCSLLFGYDWVGIPLVYTQVVTLAVYTFFFACLIGRQFLDPTKGYVGHDLDLYVPIFTLLQ FFFYAGWLKVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLA AADYCIPSFLGSTIQMGLSGSNFPAEDWLWNYEKHGNRHSVMRRVKRFLSTHEHPGSPRRRRSFGRQASD SSMFLPPSPARDLLDVPSRNPHRGSPTRKQSRSQEGSPKLHSSMGELSTIRETSRTSTLQSLSPQSSVRS SPTKMPQVPEVLITAAEAPAFSADSHQHDSTTSILSLEFTGVQPSGTEQQVEPSGTPPGDPNPQTTSAST ERDLFKFEEDLEDDRFPKRWSLPEFLESRHTSLGNLGPDPVSPRDALLLPDTETPSETNGIHPGAGSALA PDILYLMESLDKETDILEFNNEHTGESPKGTPQRPRTWF TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001007583 |
ORF Size | 2007 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001007583.1, NP_001007584.1 |
RefSeq Size | 2719 bp |
RefSeq ORF | 2010 bp |
Locus ID | 382427 |
UniProt ID | Q6H1V1 |
Cytogenetics | 10 D2 |
Gene Summary | Forms calcium-sensitive chloride channels. Permeable to bicarbonate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220299 | Best3 (untagged) - Mouse bestrophin 3 (Best3), (10ug) |
USD 675.00 |
|
MR220298 | Best3 (Myc-DDK-tagged) - Mouse bestrophin 3 (Best3) |
USD 613.00 |
|
MR220298L3 | Lenti ORF clone of Best3 (Myc-DDK-tagged) - Mouse bestrophin 3 (Best3) |
USD 913.00 |
|
MR220298L4 | Lenti ORF clone of Best3 (mGFP-tagged) - Mouse bestrophin 3 (Best3) |
USD 913.00 |
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