Cdk13 (NM_001081058) Mouse Tagged ORF Clone
CAT#: MG219227
- TrueORF®
Cdk13 (tGFP-tagged) - Mouse cyclin-dependent kinase 13 (Cdk13) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001081058" in other vectors (2)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Cdk13 |
Synonyms | 2310015O17Rik; Cdc2l5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG219227 representing NM_001081058
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGTAGCTCGGACACGGCGCTGGGGGGAGGCGGGGGCCTGAGCTGGGCCGAGAAGAAGTTGGAGG AGCGCCGCAAGCGGAGGCGATTCCTGTCCCCTCAGCAGCCGCCGCTGCTGTTGCCGCTCCTGCAGCCGCA GCTCCTGCAACCGCCGCCGCCCCCGCCGCCTCTGCTCTTCCTGGCTGCCCCCGGCGCGGCCGCCGCCGCA GCCGCCGCCGCCGCGGCCTCCTCCTCTTGCTTCAGCCCGGGCCCTCCTCTGGAGGTCAAGCGGCTGGCGA GAGGCAAGAGGCGCCCCGGAGGGCGGCAGAAGCGGCGCCGCGGGCCCCGCGCCGGGCAGGAGGCGGAGAA GCGCCGGGTCTTCTCGCTGCCCCAGCCGCAGCAGGACGGCGGTGGCGGTGCCAGTAGCGGCGGGGGTGTG ACCCCGCTGGTGGAGTACGAGGATGTGAGCTCCCAGTCCGAGCAGGGGCTGCTGCTGGGGGGCGCCAGCG CGGCAACGGCGGCGACGGCTGCCGGGGGAACGGGGGGCAACGGCGGGAGTCCGGCCTCCTCCTCCGGCAC GCAGAGGCGCGCGGAGGGGTCGGAGCGCAGGCCGCGCCGGGACCGCCGCAGCAGCAGCGGCCGCAGCAAG GAGCGCCACCGCGAGCACCGGCGGCGGGACGGGACGCGCAGCGGCAGCGAGGCCTCAAAGGCCCGCAGCC GCCACGGACACAGCGGCGAGGAGCGGGCGGAGGCCGCCAAGAGCGGCAGCAGCAGCAGCAGCGGCGGCCG CCGCAAGAGCGCGTCGGCTACGTCCAGCAGCAGTAGCAGCCGCAAGGACCGGGACCTCAAGGCCCACCGC AGCCGGACTAAGTCGTCCAAGGAACCGCCCTCGGCCTACAAGGAGCCGCCCAAGGCCTACCGGGAGGACA AGAGCGAGCCGAAGGCCTACAGGCGGCGGCAGCGGTCCCTGAGCCCGCTGGGAGGCCGGGACGAAAGCCC GGTGTCCCACAGGGCCTCGCAGAGCCTTCGGAGCCGCAAGTCCCCCAGTCCGGCGGGAGGTGGCAGCAGT CCTTATTCCCGGCGGCTGCCGCGCTCCCCGAGCCCCTATAGCCGGCGCCGCTCGCCCAGCTACAGCCGCC ACAGCTCCTACGAGCGGGGCGGCGACGTATCCCCCAGCCCGTACAGCAGCAGCAGCTGGCGGCGCTCGCG CAGCCCCTACAGCCCAGTACTCAGACGATCTGCCAAATCCCGAAGCAGAAGCCCATATTCATCTAGGCAC TCAAGATCTCGGAGCAGGCACAGATTGTCTAGATCAAGAAGTCGTCATTCAAGCATTTCTCCTAGCACAC TAACTCTGAAGAGTAGCTTGGCAGCTGAATTGAACAAGAATAAAAAGGCACGAGCTGCAGAGGCAGCAAG AGCTGCAGAGGCAGCAAAAGCTGCAGAAGCTGCTAAGGCTGCTGAAGCAGCTGCCAAAGCTGCCAAAGCC TCAAATGCTTCTACACCTACCAAGGGGAACACAGAAACTGGTGCCAGTGTCTCACAGACAAACCATGTGA AGGAAGTCAAAAAACTTAAAACTGAGCATGCACCTTCTCCTTCAAGTGGTGGGACCGTCAAAAGCGACAA AGCAAAAACAAAGCCACCGCTTCAAGTAACAAAGGTAGACAATAATTTGACAGTAGAGAAAGCCACCAAG AAAACAGTTGTTGGGAAGGAGAGTAAACCTGCTGCTACAAAGGAAGAACCAGTTTCCACTAAAGAGAAAA GCAAGCCACTCACACCAAGCACAGGAGCCAAGGAGAAGGAGCAGCATGTGGCTTTAGTGACCTCTACGTT ACCGCCATTACCTTTGCCTCCCATGCTGCCTGAAGATAAAGATGCTGATAGCTTAAGAGGCAACATTTCT GTCAAAGCAGTTAAAAAAGAAGTAGAAAAGAAACTCCGATGTCTGCTTGCTGATTTACCATTGCCCCCTG AGTTACCAGGAGGAGATGATCTTTCCAAGAGTCCAGAGGAGAAGAAAACAGCAGCACAGTTACATAGCAA ACGAAGGCCTAAAATATGTGGGCCTCGCTATGGTGAAATCAAAGAAAAAGATATTGACTGGGGGAAACGC TGCGTGGATAAATTTGATATCATCGGAATTATTGGAGAAGGTACTTATGGACAAGTTTACAAAGCCAGGG ACAAAGACACGGGAGAAATGGTAGCCTTAAAGAAAGTACGTCTGGATAATGAAAAGGAGGGTTTCCCAAT TACAGCAATTAGAGAAATTAAAATTCTTCGGCAACTCACCCACCAGAGTATCATCAATATGAAGGAAATC GTGACTGATAAAGAAGATGCTTTGGATTTTAAGAAAGACAAAGGTGCATTTTACCTGGTGTTTGAATATA TGGACCATGATCTGATGGGACTGCTGGAATCAGGCTTGGTTCATTTTAATGAAAACCATATAAAATCTTT TATGAGACAGCTCATGGAAGGCCTGGATTATTGTCATAAGAAGAACTTTTTGCATAGAGATATTAAATGT TCAAATATCCTTCTAAATAATAGAGGACAGATAAAACTTGCAGATTTTGGACTTGCTCGTTTATATAGCT CAGAAGAAAGTCGCCCATATACTAACAAGGTCATTACTTTGTGGTATCGTCCACCTGAATTGCTCTTGGG AGAAGAACGATATACACCAGCCATTGATGTATGGAGCTGTGGATGTATCCTTGGTGAACTCTTCACTAAA AAACCTATATTTCAAGCAAACCAGGAACTTGCACAGCTAGAGCTAATAAGCCGTATATGTGGGAGTCCAT GTCCTGCAGTGTGGCCTGATGTAATCAAACTGCCATATTTCAACACCATGAAACCAAAGAAGCAATATCG GCGGAAGTTAAGAGAAGAATTTGTTTTCATCCCCGCAGCTGCACTCGACTTATTCGATTACATGCTTGCC TTGGATCCCAGTAAGCGCTGCACTGCTGAGCAGGCTCTTCAGTGTGAGTTCCTGCGAGACGTGGAACCCT CCAAAATGCCTCCACCAGACCTTCCTTTGTGGCAAGATTGTCATGAATTATGGAGTAAAAAGAGAAGAAG ACAGAAACAGATGGGCATGACTGATGATCTTTCCACAATCAAAGCCCCTAGGAAGGACCTGTCTCTGGGC TTAGATGACAGCAGAACTAACACACCCCAGGGTGTGCTGCCACCCGCACAGTTGAAATCTCAGAGCAACT CAAATGTAGCACCTGTAATAACAGGCCCTGGACAGCCGTTAAACCACAGTGAATTGGCAATTCTTCTAAA CCTACTACAATCTAAATCAAGTGTTAATATGGCTGATTTTGTCCAAGTGTTGAACATTAAGGTAAACTCT GAGACTCAACAGCAGCTAAATAAAATAAACCTTCCTGCTGGAATTTTGGCAACAGGTGAAAAACAGACAG ATCCATCAACACCACAACAGGAGTCTTCAAAATCATTGGGAGGAGTTCAGCCTTCACAGACCATCCAGCC TAAAGTGGAAACTGATGCTGCCCAGGCTGCTGTGCAGAGTGCATTTGCAGTCCTCTTGACTCAGTTAATA AAGGCCCAACAGTCCAAACAGAAAGATGCCATGCTAGAGGAAAGGGAAAATGGATCAGGACATGAAGCTC CATTGCAACTCAGGCCTCCTCTAGAACCGAGCACTCCTGGATCCGGGCAAGATGACCTCATCCAGCACCA AGACAGGAGGATATTGGAGCTGACACCAGAACCAGACCGGCCTCGAATTCTGCCTCCTGATCAACGGCCT CCTGAACCTCCTGAACCACCACCAGTTACTGAGGAGGACCTAGATTATCGGACAGAAAACCAGCATGTTC CTACCACTAGTTCTTCATTAACTGACCCACATGCTGGAGTGAAGGCAGCCCTCTTACAGCTGCTTGCTCA GCATCAGCCCCAGGATGATCCCAAAAGAGAAGGTGGTATCGATTATCCCACAGGAGACACATATGTGCCC AGTTCAGACTATAAGGACAACTTTGGATCTTCTTTCTCTGCCGCTCCTTACGTTAGCAGTGATGGTCTAG GAAGTAGCTCCGCTGCTGCACCATTGGAAGCACGTAGTTTCATTGGAAACTCAGATATTCAGTCTCTGGA TAACTACAGTACTGCTTCATCTCACACTGGTGGTCCACCTCAAACTTCTGCCTTTACTGAGTCGTTTGCC AGTTCAGTAGCTGGATATGGAGACATTTACCTCAATGCTGGTCCCATGTTGTTTAGTGGAGACAAGGACC ATAGATTTGAATATAGCCATGGTCCTATCACAGTCCTCACAAACAGCAATGACCCTTCCACAGGGCCAGA GAGTACTCATCCCTTGCCAGCAAAGATGCACAACTATAACTATGGTGGTAACTTACAGGAAAATCCAGGT GGCCCTAGCCTCATGCATGGACAGACCTGGACTTCTCCTGCCCAAGGACCTGGATATTCACAAGGATACA GGGGACACATTAGCACATCAGCTGGCAGAGGTCGAGGCAGAGGGTTACCATAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219227 representing NM_001081058
Red=Cloning site Green=Tags(s) MPSSSDTALGGGGGLSWAEKKLEERRKRRRFLSPQQPPLLLPLLQPQLLQPPPPPPPLLFLAAPGAAAAA AAAAAASSSCFSPGPPLEVKRLARGKRRPGGRQKRRRGPRAGQEAEKRRVFSLPQPQQDGGGGASSGGGV TPLVEYEDVSSQSEQGLLLGGASAATAATAAGGTGGNGGSPASSSGTQRRAEGSERRPRRDRRSSSGRSK ERHREHRRRDGTRSGSEASKARSRHGHSGEERAEAAKSGSSSSSGGRRKSASATSSSSSSRKDRDLKAHR SRTKSSKEPPSAYKEPPKAYREDKSEPKAYRRRQRSLSPLGGRDESPVSHRASQSLRSRKSPSPAGGGSS PYSRRLPRSPSPYSRRRSPSYSRHSSYERGGDVSPSPYSSSSWRRSRSPYSPVLRRSAKSRSRSPYSSRH SRSRSRHRLSRSRSRHSSISPSTLTLKSSLAAELNKNKKARAAEAARAAEAAKAAEAAKAAEAAAKAAKA SNASTPTKGNTETGASVSQTNHVKEVKKLKTEHAPSPSSGGTVKSDKAKTKPPLQVTKVDNNLTVEKATK KTVVGKESKPAATKEEPVSTKEKSKPLTPSTGAKEKEQHVALVTSTLPPLPLPPMLPEDKDADSLRGNIS VKAVKKEVEKKLRCLLADLPLPPELPGGDDLSKSPEEKKTAAQLHSKRRPKICGPRYGEIKEKDIDWGKR CVDKFDIIGIIGEGTYGQVYKARDKDTGEMVALKKVRLDNEKEGFPITAIREIKILRQLTHQSIINMKEI VTDKEDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFNENHIKSFMRQLMEGLDYCHKKNFLHRDIKC SNILLNNRGQIKLADFGLARLYSSEESRPYTNKVITLWYRPPELLLGEERYTPAIDVWSCGCILGELFTK KPIFQANQELAQLELISRICGSPCPAVWPDVIKLPYFNTMKPKKQYRRKLREEFVFIPAAALDLFDYMLA LDPSKRCTAEQALQCEFLRDVEPSKMPPPDLPLWQDCHELWSKKRRRQKQMGMTDDLSTIKAPRKDLSLG LDDSRTNTPQGVLPPAQLKSQSNSNVAPVITGPGQPLNHSELAILLNLLQSKSSVNMADFVQVLNIKVNS ETQQQLNKINLPAGILATGEKQTDPSTPQQESSKSLGGVQPSQTIQPKVETDAAQAAVQSAFAVLLTQLI KAQQSKQKDAMLEERENGSGHEAPLQLRPPLEPSTPGSGQDDLIQHQDRRILELTPEPDRPRILPPDQRP PEPPEPPPVTEEDLDYRTENQHVPTTSSSLTDPHAGVKAALLQLLAQHQPQDDPKREGGIDYPTGDTYVP SSDYKDNFGSSFSAAPYVSSDGLGSSSAAAPLEARSFIGNSDIQSLDNYSTASSHTGGPPQTSAFTESFA SSVAGYGDIYLNAGPMLFSGDKDHRFEYSHGPITVLTNSNDPSTGPESTHPLPAKMHNYNYGGNLQENPG GPSLMHGQTWTSPAQGPGYSQGYRGHISTSAGRGRGRGLPY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001081058 |
ORF Size | 4533 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001081058.2, NP_001074527.1 |
RefSeq Size | 7081 bp |
RefSeq ORF | 4536 bp |
Locus ID | 69562 |
UniProt ID | Q69ZA1 |
Cytogenetics | 13 A2 |
Gene Summary | Cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. Has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. Required for RNA splicing, probably by phosphorylating SRSF1/SF2. Required during hematopoiesis.[UniProtKB/Swiss-Prot Function] |
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