Dock8 (NM_028785) Mouse Tagged ORF Clone

CAT#: MG219092

Dock8 (tGFP-tagged) - Mouse dedicator of cytokinesis 8 (Dock8), (10ug)

Product Images

Circular map for MG219092

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dock8
• Synonyms: 1200017A24Rik; 5830472H07Rik; A130095G14Rik; AI461977
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219092 representing NM_028785
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGCTGCCGAGCGCCGAGCGCCGCGCCTTCGCGCTCAAGATCAACAGGTATTCATCGTCAGAAA
  TAAGGAAGCAGTTTACGCTCCCACCCAACCTCGGACAGTACCATCGGCACAGTATCAGTACATCTGGTTT
  CCCCTCTCTTCAGCTACCTCAGCTTTATGAGCCTGTCGAGCCAGTGGACTTTGAAGGACTCGTGATGACA
  CACTTAAACAGCTTGGATGCAGAGCTGGCCCAGGAGCTGGGGGACCTCACCGATGACGACCTGCATGTGG
  CCTTCACACCCAAAGAATGTAGGACTTTGCAGCACTCTCTGCCAGAGGAAGGAGTTGAACTGGATCCTCA
  CGTCAGAGACTGTGTTCAGACCTATATTCGAGAGTGGCTGATTGTAAACCGGAAAAACCAAGGAAGTTCA
  GAGTTTTGCAGCTTTAAAAAGACGGGATCTCGCAGAGATTTTCAGAAGACGCTTCAGAAACAGACGTTTG
  AGTCAGAAACCTTGGAGTGCAGTGAACCGGACACTCAGACAGGACCCCGTCATCCCTTAAACGTGCTGTG
  TGACGTGTCTGGGAAGGGCCCCCTCACTTCTTGTGACTTCGACCTCCGCAGCCTGCAGCCTGATGAGCGG
  CTGGAAAACCTGCTCCAGCTTGTGAGCGCTGAGGACTTTGAGAAGGAGAAGGAGGAGGCCCGCAAGACCA
  ATCGGCCGGCTGAGCTCTTTGCCCTCTATCCGCCCGTGGATGAGGAGGATGCTGTGGAAATACGTCCAGT
  ACCTGAATGTCCTAAGGAACATCTGGGCAACAGAATATTGGTCAAGGTGCTGACTCTGAAGTTTGAGATT
  GAAATTGAACCTCTGTTTGCCAGTATTGCCCTCTATGACGTTAAAGAAAGGAAAAAGATCTCAGAAAATT
  TCCACTGTGACCTGAACTCCGACCAGTTCAAAGGGTTTCTGCGAGCTCACACACCCTCGATTGACCCATC
  GAGTCAGGCTAGGTCTGCCGTGTTCTCTGTCACCTACCCATCTTCAGACATCTACCTGGTTGTCAAGATT
  GAAAAGGTCCTTCAGCAAGGAGAGATTGCAGACTGTGCAGAACCCTACATGATCATCAAAGAAAGCGATG
  GTGGAAAGAGTAAAGAAAAGGTTGAAAAACTAAAACTTCAAGCTGAATCCTTCTGCCAACGTTTGGGGAA
  ATACCGGATGCCCTTCGCCTGGGCCCCCATTAGCTTAGCAAGCTTCTTCAACATCTCCACCCTTGAAAGG
  GAGAGCACAGATGTGGAGCCTGGGGTTGGGAGGAACTCTGTGGGTGAGAAGAGGAGCTTGTCCCAATCCA
  GGAGGCCCTCTGAGCGAACCCTCTCCTTGGAGGAAAATGGAGTTGGATCCAACTTCAAAGCCACCACCTT
  GGCCACCAACATCTTCTTCAAACAGGAGGGAGATCGCCTTAGTGATGAAGACTTGTTCAAGTTTTTAGCT
  GACTACAAGAGATCTTCATCCCTACAGCGAAGAGTCAAATCCATCCCAGGCTCACTGAGGCTGGAGATAT
  CCCCAGCTCCCGACGTGATGAACTGCTGCCTGACGCCCGAGATGCTGCCAGTCAAACCTTTTCCTGAAAA
  TCGGACGCGTCCACACAAGGAGATTTTGGAATTTCCGATCCGGGAGGTGTACGTCCCTCACACTGTGTAC
  AGAAACCTTCTGTACGTATACCCCCAGCGACTGAACTTCGCTAGCAAGCTAGCATCTGCCCGGAACATCA
  CAATAAAGATTCAGTTTATGTGCGGAGAAGACCCCAGCAATGCTATGCCGGTCATCTTTGGCAAGTCCAG
  TGGGCCTGAATTTCTGCAGGAAGTATATACAGCTATTACATACCATAATAAGTCTCCTGACTTTTACGAA
  GAAGTGAAAATTAAGCTCCCTGCCAAGCTCACAGTGAATCATCACCTCCTCTTCACCTTCTACCACATCA
  GCTGTCAGCAGAAGCAAGGGGCCTCCGGAGAAAGCCTTCTGGGGTACTCGTGGCTGCCGATTCTGTTAAA
  CGAACGTCTTCAAACCGGATCCTACTGTCTGCCTGTTGCCTTGGAAAAACTACCACCCAACTACTCCATA
  CATTCTGCTGAGAAAGTCCCTTTACAGAATCCTCCCATTAAGTGGGCCGAGGGCCATAAGGGAGTATTTA
  ATATTGAAGTGCAAGCTGTTTCTTCCGTCCACACCCAGGATAACCACCTGGAGAAGTTCTTCACCCTTTG
  CCACTCCCTGGAGAGCCAGGTGAGCTTCCCTATCCGTGTGCTGGACCAGAAGATCACCGAGAGCACGCTG
  GAGCACGAGCTGAAACTCAGCATCATCTGCCTCAACTCCTCCCGCCTGGAGCCCCTCGTGCTCTTCCTCC
  ACCTGGTGCTCGACAAGCTGTTCCAGCTTTCCGTGCAGCCCATGGTCATTGCTGGCCAAACAGCAAACTT
  CTCCCAGTTTGCCTTCGAGTCTGTGGTGGCCATTGCCAATAGCCTGCACAACAGCAAGGACCTGAGGAAG
  GACCAGCACGGAAGGAACTGCCTTCTGGCCTCCTATGTGCACTACGTGTTCCGGCTGCCGGAACTGCACA
  GGGATACATCCAAGTCAGGTGGCCCCATCACCGTAGTCCCCGACCCCCGATACCACACATATGGACGCAC
  ATCTGCCGCTGCAGTGAGTTCAAAGCTGATGCAGGCCCGTGTGATGAGCAGCAGCAACCCAGACCTGACT
  GGGTCACACTGTGCAGCCGATGAGGAAGTTAAGAACATCATGTCTTCAAAGATTGCCGATCGCAACTGCA
  GCCGGATGTCTTACTATTGCTCTGGCAATAGTGATGCGCCAGGTTCAACTGCAGCCCCAAGACCAGTCAG
  CAAAAAGCATTTCCATGAGGAGCTTGCCCTGCAGATGGTGGTCAGCACTGGAGTAGTGAGAGAAACGGTC
  TTCAAGTACGCCTGGTTCTTCTTTGAGCTTCTGGTGAAAAGCATGGCGCAGTATGTCCATAACCTGGATA
  AACGGGACAGTTTTCGGAGGACTCGTTTTTCTGACCGCTTCAAAGATGACATAACTACCATTGTTAATGT
  GGTCACCTCGGAGATAGCAGCCCTTTTAGTGAAACCTCAGAAGGAAAGCGAGCAGGCAGAAAAGATCAAC
  ATCAGCCTTGCCTTCTTCCTGTATGACCTCCTGTCAATCATGGACAGAGGCTTCGTGTTCAACCTCATCA
  AGCATTACTGCAGCCAGCTGTCAGCCAAGCTGAATATCCTTCCAACGCTCATCTCCATGCGGCTGGAATT
  CCTGAGGATCCTCTGCAGCCATGAGCACTACCTCAACTTGAACCTCCTCTTCATGAATACCGACACCGCA
  CCAGCATCTCCCTGCCCCTCCATATCCTCCCAGAACTCGAGTTCCTGCTCCAGTTTCCAGGACCAAAAGA
  TTGCCAGCATGTTCGATCTGACCCCGGAGTACCGGCAGCAGCACTTCCTTACAGGGCTGCTCTTCACGGA
  GCTGGCTGTTGCCCTGGATGCTGAGGGGGATGGAATTAGCAGAGTACAGAGAAAAGCCGTGAGTGCCATC
  CACAGCCTTCTGTGTTCTCACGACCTGGATCCACGGTGTCGCAAACCGGAAGTGAAAGTCAAAATCGCCG
  CCCTTTACCTGCCGTTGGTCGGCATCATTCTGGACGCTCTGCCACAGCTCTATGACTTTACAGATGCTCG
  CAGTGGAAGGAGTCGTGCCAGTGGCTCGTATGAAGAACAAGATGTGGCCAACGGAATCAACCAGAATGTG
  GCCCTGGCCATAGCGGGGAATCACTTTAATTTGAAGACCAGTGGAGCAATGCTGTCTTCCTTGCCCTATA
  AGCAGTACAACATGCTGAATGCAGACACCACCCGCCACCTCATGATTTGCTTCCTGTGGATCATGAAAAA
  TGCTGATCAGAGCCTCATCAGGAAGTGGATCGCTGACCTGCCTTCCATGCAGCTCAATAGGATTCTGGAC
  CTGCTGTTCATCTGTGTCTCCTGCTTTGAATACAAGGGAAAGCAGAGTTCTGACAAAGTCAGTAACCAGG
  TCCTGCAGAAGTCAAGAGACGTCAAGGCCAAGCTGGAAGAGGCCCTGCTCCGTGGGGAAGGAGCCCGTGG
  GGAGATGATGCGCCGTCGCATTCCAGGGACTGACCGGTTTCCAGGCATAAATGAAAATCTGAGATGGAGG
  AAAGAGCAGACACAGTGGCGGCAGGCTAATGAGAAGCTGGACAAAACAAAGGCAGAGTTAGATCAAGAAG
  CCTTGATCAGTGGCAACCTGGCTACAGAAGCTAATTTGATCATCCTGGATATGCAGGAGAACATCATCCA
  GGCAAGCTCCGCCCTGGACTGTAAAGACAGCCTGCTGGGAGGTGTCCTCCGGGTCCTGGTGAATTCTCTG
  AGCTGTGACCAGAGCACCACCTACCTGACTCACTGTTTCGCAACCCTCCGAGCCCTCATCGCCAAGTTTG
  GAGACCTGCTGTTCGAGGAGGAGATGGAGCAGTGTGCTGACCTGTGTCAGCGGGTGCTACATCACTGCAG
  CAGCAGCATGGACGTCACACGGAGCCAAGCCTGCGCCACCCTCTACCTCCTCATGCGGTTCAGCTTCGGA
  GCCACCAGTAACTTTGCAAGGGTAAAGATGCAAGTGACCATGGCACTGGCATCCCTGGTAGGCAAAGCAC
  CAGACTTCAACGAAGAGCACCTGAGAAGGTCCTTAAGGACAATTTTGGCCTATTCAGAAGAGGACACGGC
  CATGCAGACAACTCCTTTTCCCATGCAGGTGGAGGAACTTCTCTGCAATCTGAACAGCATTCTGTACGAC
  ACAGTGAAGATGAGGGAATTCCAGGAAGACCCTGAGATGCTTATGGACCTCATGTACAGAATTGCCAAGA
  GCTACCAGGCATCGCCTGACCTGCGGCTGACTTGGCTCCAGAACATGGCAGAGAAACACACTAAGAAGAA
  GTGCTTCACAGAGGCCGCCATGTGCCTGGTGCATGCAGCCGCCCTGGTGGCCGAGTACCTGAGCATGCTG
  GAGGACCACAGCTACCTGCCGGTGGGCAGCGTCAGCTTTCAGAATATTTCTTCCAATGTGCTTGAGGAGT
  CTGCAGTCTCTGATGACACCTTGTCACCTGATGAGGACGGCGTATGCTCTGGTCGGTACTTCACTGAGAG
  TGGCCTGGTGGGCCTCCTGGAGCAGGCTGCGGAGCTCTTCAGCACGGGAGGCTTGTACGAGACGGTTAAT
  GAAGTCTACAAGCTGGTCATCCCTATCCTGGAGGCACACAGAGATTTCCGGAAGCTGACCTCCACTCACG
  ACAAGCTGCAGAAGGCCTTCGATAACATCATCAACAAGGACCATAAGAGGATGTTTGGGACCTACTTCCG
  AGTTGGTTTCTACGGATCCCGATTTGGGGATTTGGATGAGCAGGAGTTCGTGTACAAGGAACCCGCAATC
  ACGAAGCTCCCGGAGATCTCACATAGACTAGAGGGATTTTATGGCCAGTGTTTCGGTGCAGAGTTTGTGG
  AAGTGATAAAAGACTCTACTCCAGTGGACAAAACCAAGTTGGATCCTAACAAGGCCTACATTCAGATCAC
  TTTTGTGGAGCCTTACTTTGATGAATATGAGATGAAAGACCGGGTGACCTACTTCGAGAAGAATTTCAAC
  CTCCGGAGGTTCATGTACACCACCCCCTTCACCCTGGAGGGGAGACCCCGGGGCGAGCTTCATGAGCAAC
  ACCGCAGAAACACCGTGCTCACCACCATGCACGCCTTCCCCTACATCAAGACCAGGATCCGAGTCAGCCA
  GAAAGAGGAGTTCGTTTTGACTCCGATTGAGGTTGCCATTGAAGATATGAAGAAGAAGACCCTGCAGTTA
  GCCGTGGCCACTCACCAGGAGCCCCCTGATGCAAAGATGCTGCAAATGGTACTGCAGGGCTCTGTAGGAG
  CCACTGTAAATCAGGGACCACTGGAGGTGGCCCAAGTGTTCTTGGCTGAAATTCCAGCTGACCCAAAGCT
  CTACCGACATCACAACAAGCTGAGGTTGTGCTTCAAGGAGTTCATAATGCGATGCGGAGAGGCCGTGGAG
  AAGAACAGGCGACTCATCACCGCAGAGCAGCGGGAGTACCAGCAGGAGCTGAAGAAGAACTACAACAAGC
  TGAGAGACAGCCTCAGGCCCATGATTGAGCGGAAAATCCCAGAGCTCTACAAGCCCATATTCAGAGTTGA
  CAGTCAGAAGAGGGACTCTTTCCACAGATCTAGTTTCAGGAAATGTGAAACCCAGTTGTCACAGGGCAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219092 representing NM_028785
  Red=Cloning site Green=Tags(s)
  
  MATLPSAERRAFALKINRYSSSEIRKQFTLPPNLGQYHRHSISTSGFPSLQLPQLYEPVEPVDFEGLVMT
  HLNSLDAELAQELGDLTDDDLHVAFTPKECRTLQHSLPEEGVELDPHVRDCVQTYIREWLIVNRKNQGSS
  EFCSFKKTGSRRDFQKTLQKQTFESETLECSEPDTQTGPRHPLNVLCDVSGKGPLTSCDFDLRSLQPDER
  LENLLQLVSAEDFEKEKEEARKTNRPAELFALYPPVDEEDAVEIRPVPECPKEHLGNRILVKVLTLKFEI
  EIEPLFASIALYDVKERKKISENFHCDLNSDQFKGFLRAHTPSIDPSSQARSAVFSVTYPSSDIYLVVKI
  EKVLQQGEIADCAEPYMIIKESDGGKSKEKVEKLKLQAESFCQRLGKYRMPFAWAPISLASFFNISTLER
  ESTDVEPGVGRNSVGEKRSLSQSRRPSERTLSLEENGVGSNFKATTLATNIFFKQEGDRLSDEDLFKFLA
  DYKRSSSLQRRVKSIPGSLRLEISPAPDVMNCCLTPEMLPVKPFPENRTRPHKEILEFPIREVYVPHTVY
  RNLLYVYPQRLNFASKLASARNITIKIQFMCGEDPSNAMPVIFGKSSGPEFLQEVYTAITYHNKSPDFYE
  EVKIKLPAKLTVNHHLLFTFYHISCQQKQGASGESLLGYSWLPILLNERLQTGSYCLPVALEKLPPNYSI
  HSAEKVPLQNPPIKWAEGHKGVFNIEVQAVSSVHTQDNHLEKFFTLCHSLESQVSFPIRVLDQKITESTL
  EHELKLSIICLNSSRLEPLVLFLHLVLDKLFQLSVQPMVIAGQTANFSQFAFESVVAIANSLHNSKDLRK
  DQHGRNCLLASYVHYVFRLPELHRDTSKSGGPITVVPDPRYHTYGRTSAAAVSSKLMQARVMSSSNPDLT
  GSHCAADEEVKNIMSSKIADRNCSRMSYYCSGNSDAPGSTAAPRPVSKKHFHEELALQMVVSTGVVRETV
  FKYAWFFFELLVKSMAQYVHNLDKRDSFRRTRFSDRFKDDITTIVNVVTSEIAALLVKPQKESEQAEKIN
  ISLAFFLYDLLSIMDRGFVFNLIKHYCSQLSAKLNILPTLISMRLEFLRILCSHEHYLNLNLLFMNTDTA
  PASPCPSISSQNSSSCSSFQDQKIASMFDLTPEYRQQHFLTGLLFTELAVALDAEGDGISRVQRKAVSAI
  HSLLCSHDLDPRCRKPEVKVKIAALYLPLVGIILDALPQLYDFTDARSGRSRASGSYEEQDVANGINQNV
  ALAIAGNHFNLKTSGAMLSSLPYKQYNMLNADTTRHLMICFLWIMKNADQSLIRKWIADLPSMQLNRILD
  LLFICVSCFEYKGKQSSDKVSNQVLQKSRDVKAKLEEALLRGEGARGEMMRRRIPGTDRFPGINENLRWR
  KEQTQWRQANEKLDKTKAELDQEALISGNLATEANLIILDMQENIIQASSALDCKDSLLGGVLRVLVNSL
  SCDQSTTYLTHCFATLRALIAKFGDLLFEEEMEQCADLCQRVLHHCSSSMDVTRSQACATLYLLMRFSFG
  ATSNFARVKMQVTMALASLVGKAPDFNEEHLRRSLRTILAYSEEDTAMQTTPFPMQVEELLCNLNSILYD
  TVKMREFQEDPEMLMDLMYRIAKSYQASPDLRLTWLQNMAEKHTKKKCFTEAAMCLVHAAALVAEYLSML
  EDHSYLPVGSVSFQNISSNVLEESAVSDDTLSPDEDGVCSGRYFTESGLVGLLEQAAELFSTGGLYETVN
  EVYKLVIPILEAHRDFRKLTSTHDKLQKAFDNIINKDHKRMFGTYFRVGFYGSRFGDLDEQEFVYKEPAI
  TKLPEISHRLEGFYGQCFGAEFVEVIKDSTPVDKTKLDPNKAYIQITFVEPYFDEYEMKDRVTYFEKNFN
  LRRFMYTTPFTLEGRPRGELHEQHRRNTVLTTMHAFPYIKTRIRVSQKEEFVLTPIEVAIEDMKKKTLQL
  AVATHQEPPDAKMLQMVLQGSVGATVNQGPLEVAQVFLAEIPADPKLYRHHNKLRLCFKEFIMRCGEAVE
  KNRRLITAEQREYQQELKKNYNKLRDSLRPMIERKIPELYKPIFRVDSQKRDSFHRSSFRKCETQLSQGS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_028785
• ORF Size: 6300 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_028785.3, NP_083061.2
• RefSeq Size: 7810 bp
• RefSeq ORF: 6303 bp
• Locus ID: 76088
• UniProt ID: Q8C147
• Cytogenetics: 19 B
• Gene Summary: Guanine nucleotide exchange factor (GEF) which specifically activates small GTPase CDC42 by exchanging bound GDP for free GTP (PubMed:28028151, PubMed:22461490). During immune responses, required for interstitial dendritic cell (DC) migration by locally activating CDC42 at the leading edge membrane of DC (PubMed:22461490, PubMed:25713392). Required for CD4(+) T-cell migration in response to chemokine stimulation by promoting CDC42 activation at T cell leading edge membrane (PubMed:28028151). Is involved in NK cell cytotoxicity controlling polarization of microtubule-organizing center (MTOC), and possibly regulating CCDC88B-mediated lytic granule transport to MTOC during cell killing (By similarity).[UniProtKB/Swiss-Prot Function]