Eef1akmt4-ece2 (NM_177941) Mouse Tagged ORF Clone

CAT#: MG219025

Ece2 (tGFP-tagged) - Mouse endothelin converting enzyme 2 (Ece2) transcript variant 1, (10ug)

Product Images

Circular map for MG219025

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eef1akmt4-ece2
• Synonyms: 1810009K13Rik; 6330509A19Rik; 9630025D12Rik; BB127715; Ece-2; Eef1akmt4
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219025 representing NM_177941
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTCTCCCCGGACTCCGGTGTCCCCTCCGGAGTTACCGGAGAAGAACTTCCAGTACCGCCAAGTCC
  AGTACTGGGACCAGCGCTACAAAGACGCGGCCGACTCTGGACCCTACGAGTGGTTCGGAGACTTCGCTTC
  CTTCCGTGCCCTCCTAGAGCCGGAGCTGTGTCCTGAGGACCGTATCCTCGTGCTAGGTTGCGGGAACAGT
  GCCTTGAGCTATGAGCTGTTCCTTGGAGGCTTCCCTAATGTGACCAGTGTGGATTACTCTCCAGTAGTGG
  TGGCAGCCATGCAAGTTCGCTATGCCCATGTACCCAGTCTACGCTGGGAGACCATGGATGTTCGAGCACT
  GGACTTCCCTAGTGGGTCTTTTGATGTTGTCCTCGAGAAGGGTACTCTGGATGCCATGCTGGCTGGGGAG
  CCAGATCCCTGGAATGTATCTTCTGAAGGTGTCCACACTGTGGACCAGGTGCTGAGTGAGATGGTGGAGT
  ACAAACGTGCTAAGCTTCGGGATGAAGAATCACCAGAGATTACAGTTGAAGGCAGGGCCACCCGGGACTC
  ACTGGAGGTGGGATTCCAGAAGAGGACAAGACAACTGTTTGGTTCACACACACAGTTGGAGCTGGTCTTG
  GCAGGCCTCATTCTAGTGTTGGCTGCCCTTCTTTTGGGCTGCCTCGTGGCTCTGTGGGTCCACAGAGACC
  CAGCCCATAGCACCTGCGTCACAGAAGCCTGCATTCGAGTAGCTGGAAAAATCCTGGAGTCTCTAGACCG
  TGGGGTGAGCCCCTGTCAGGACTTTTACCAGTTCTCCTGTGGAGGCTGGATTCGAAGAAACCCTCTACCC
  AATGGACGTTCTCGCTGGAACACCTTCAACAGCCTCTGGGACCAGAACCAGGCCATACTGAAGCACCTAC
  TTGAGAACACCACTTTCAATTCCAGCAGTGAAGCTGAGAGGAAGACTCGGAGTTTCTACCTGTCCTGCCT
  ACAGTCGGAGCGCATTGAGAAGCTAGGAGCCAAGCCACTTAGAGACCTCATTGACAAGATCGGTGGTTGG
  AACATAACGGGGCCTTGGGACGAGGACAGCTTCATGGATGTGCTCAAGGCAGTCGCAGGGACCTACAGAG
  CCACCCCCTTCTTCACCGTCTACGTCAGTGCTGATTCTAAGAGTTCTAACAGCAATATCATCCAGGTGGA
  CCAGTCTGGGCTTTTTCTGCCCTCTCGAGATTACTACCTAAATAGAACTGCCAATGAGAAAGTTCTCACT
  GCCTACCTGGACTACATGGTGGAGCTGGGAGTGCTGCTGGGTGGACAGCCGACCTCCACTCGGGAGCAGA
  TGCAGCAGGTGCTGGAGCTGGAGATACAGCTGGCTAACATCACTGTGCCCCAGGACCAGCGGCGTGATGA
  GGAGAAGATCTATCACAAGATGAGCATCTCAGAGCTGCAGGCTCTCGCGCCCGCCGTGGACTGGCTGGAG
  TTCCTTTCTTTCTTGTTATCGCCACTTGAGTTGGGTGATTCTGAGCCTGTGGTGGTGTATGGGACTGAGT
  ATTTACAGCAGGTGTCGGAGCTCATCAACCGTACTGAACCAAGCATCCTGAACAATTACCTAATTTGGAA
  CCTGGTACAGAAGACGACCTCAAGCCTTGACCAGCGCTTTGAGACTGCACAGGAGAAACTGCTGGAGACC
  CTCTACGGTACCAAGAAGTCCTGCACTCCGAGGTGGCAGACCTGCATCTCCAATACAGATGATGCCCTTG
  GCTTTGCTCTGGGTTCACTCTTTGTGAAAGCCACATTTGACCGACAAAGCAAGGAAATCGCCGAGGGGAT
  GATCAATGAAATCCGCTCTGCTTTTGAGGAGACCCTGGGAGACTTGGTTTGGATGGATGAGAAGACCCGG
  CTGGCAGCCAAGGAGAAAGCAGATGCCATCTATGATATGATTGGTTTCCCTGATTTCATCCTGGAGCCCA
  AAGAGCTGGATGATGTTTATGATGGGTATGAAGTCTCTGAAGATTCTTTTTTCCAAAACATGTTGAATCT
  GTACAACTTCTCAGCTAAGGTGATGGCTGACCAGCTCCGCAAACCTCCCAGCCGAGACCAGTGGAGCATG
  ACACCTCAGACCGTGAACGCTTACTACCTTCCAACCAAGAATGAAATCGTCTTCCCTGCTGGCATCTTGC
  AGGCCCCCTTCTATGCTCACAACCATCCAAAGGCCTTGAACTTTGGTGGCATCGGCGTAGTGATGGGCCA
  TGAGTTGACACATGCCTTTGATGACCAAGGGCGTGAGTATGACAAAGAAGGGAATCTGCGTCCTTGGTGG
  CAGAATGAGTCACTGACGGCTTTCCAGAACCATACAGCCTGCATGGAAGAACAGTACAGCCAGTACCAGG
  TCAATGGAGAGAGGCTCAATGGACTCCAGACCCTGGGGGAAAACATCGCCGATAATGGGGGCCTTAAGGC
  TGCTTACAATGCTTACAAAGCATGGCTGAGAAAGCATGGGGAGGAGCAGCCGCTGCCTGCTGTGGGGCTC
  ACCAATCACCAGCTTTTCTTCGTGGGATTTGCTCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTC
  ACGAGGGGCTGGTGACCGACCCCCACAGCCCTGCCCGTTTCCGAGTGCTGGGCACTCTCTCCAACTCCCG
  AGACTTCCTTCGGCACTTCGGCTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTATGTGAGGTGTGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219025 representing NM_177941
  Red=Cloning site Green=Tags(s)
  
  MASPRTPVSPPELPEKNFQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNS
  ALSYELFLGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDVRALDFPSGSFDVVLEKGTLDAMLAGE
  PDPWNVSSEGVHTVDQVLSEMVEYKRAKLRDEESPEITVEGRATRDSLEVGFQKRTRQLFGSHTQLELVL
  AGLILVLAALLLGCLVALWVHRDPAHSTCVTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRRNPLP
  NGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGW
  NITGPWDEDSFMDVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLT
  AYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPAVDWLE
  FLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLET
  LYGTKKSCTPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTR
  LAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSM
  TPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWW
  QNESLTAFQNHTACMEEQYSQYQVNGERLNGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGL
  TNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177941
• ORF Size: 2730 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177941.1, NP_808810.1
• RefSeq Size: 3436 bp
• RefSeq ORF: 2733 bp
• Locus ID: 110599584
• UniProt ID: P0DPE0
• Cytogenetics: 16 B1
• Gene Summary: This locus represents naturally occurring readthrough transcription between the adjacent genes eukaryotic translation elongation factor 1 alpha lysine specific methyltransferase 4 (GeneID:110599566) and endothelin converting enzyme 2 (GeneID:107522). The readthrough transcript representing this gene encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Jul 2017]