Eef1akmt4-ece2 (NM_177941) Mouse Tagged ORF Clone
CAT#: MG219025
- TrueORF®
Ece2 (tGFP-tagged) - Mouse endothelin converting enzyme 2 (Ece2) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_177941" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Eef1akmt4-ece2 |
Synonyms | 1810009K13Rik; 6330509A19Rik; 9630025D12Rik; BB127715; Ece-2; Eef1akmt4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG219025 representing NM_177941
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTCTCCCCGGACTCCGGTGTCCCCTCCGGAGTTACCGGAGAAGAACTTCCAGTACCGCCAAGTCC AGTACTGGGACCAGCGCTACAAAGACGCGGCCGACTCTGGACCCTACGAGTGGTTCGGAGACTTCGCTTC CTTCCGTGCCCTCCTAGAGCCGGAGCTGTGTCCTGAGGACCGTATCCTCGTGCTAGGTTGCGGGAACAGT GCCTTGAGCTATGAGCTGTTCCTTGGAGGCTTCCCTAATGTGACCAGTGTGGATTACTCTCCAGTAGTGG TGGCAGCCATGCAAGTTCGCTATGCCCATGTACCCAGTCTACGCTGGGAGACCATGGATGTTCGAGCACT GGACTTCCCTAGTGGGTCTTTTGATGTTGTCCTCGAGAAGGGTACTCTGGATGCCATGCTGGCTGGGGAG CCAGATCCCTGGAATGTATCTTCTGAAGGTGTCCACACTGTGGACCAGGTGCTGAGTGAGATGGTGGAGT ACAAACGTGCTAAGCTTCGGGATGAAGAATCACCAGAGATTACAGTTGAAGGCAGGGCCACCCGGGACTC ACTGGAGGTGGGATTCCAGAAGAGGACAAGACAACTGTTTGGTTCACACACACAGTTGGAGCTGGTCTTG GCAGGCCTCATTCTAGTGTTGGCTGCCCTTCTTTTGGGCTGCCTCGTGGCTCTGTGGGTCCACAGAGACC CAGCCCATAGCACCTGCGTCACAGAAGCCTGCATTCGAGTAGCTGGAAAAATCCTGGAGTCTCTAGACCG TGGGGTGAGCCCCTGTCAGGACTTTTACCAGTTCTCCTGTGGAGGCTGGATTCGAAGAAACCCTCTACCC AATGGACGTTCTCGCTGGAACACCTTCAACAGCCTCTGGGACCAGAACCAGGCCATACTGAAGCACCTAC TTGAGAACACCACTTTCAATTCCAGCAGTGAAGCTGAGAGGAAGACTCGGAGTTTCTACCTGTCCTGCCT ACAGTCGGAGCGCATTGAGAAGCTAGGAGCCAAGCCACTTAGAGACCTCATTGACAAGATCGGTGGTTGG AACATAACGGGGCCTTGGGACGAGGACAGCTTCATGGATGTGCTCAAGGCAGTCGCAGGGACCTACAGAG CCACCCCCTTCTTCACCGTCTACGTCAGTGCTGATTCTAAGAGTTCTAACAGCAATATCATCCAGGTGGA CCAGTCTGGGCTTTTTCTGCCCTCTCGAGATTACTACCTAAATAGAACTGCCAATGAGAAAGTTCTCACT GCCTACCTGGACTACATGGTGGAGCTGGGAGTGCTGCTGGGTGGACAGCCGACCTCCACTCGGGAGCAGA TGCAGCAGGTGCTGGAGCTGGAGATACAGCTGGCTAACATCACTGTGCCCCAGGACCAGCGGCGTGATGA GGAGAAGATCTATCACAAGATGAGCATCTCAGAGCTGCAGGCTCTCGCGCCCGCCGTGGACTGGCTGGAG TTCCTTTCTTTCTTGTTATCGCCACTTGAGTTGGGTGATTCTGAGCCTGTGGTGGTGTATGGGACTGAGT ATTTACAGCAGGTGTCGGAGCTCATCAACCGTACTGAACCAAGCATCCTGAACAATTACCTAATTTGGAA CCTGGTACAGAAGACGACCTCAAGCCTTGACCAGCGCTTTGAGACTGCACAGGAGAAACTGCTGGAGACC CTCTACGGTACCAAGAAGTCCTGCACTCCGAGGTGGCAGACCTGCATCTCCAATACAGATGATGCCCTTG GCTTTGCTCTGGGTTCACTCTTTGTGAAAGCCACATTTGACCGACAAAGCAAGGAAATCGCCGAGGGGAT GATCAATGAAATCCGCTCTGCTTTTGAGGAGACCCTGGGAGACTTGGTTTGGATGGATGAGAAGACCCGG CTGGCAGCCAAGGAGAAAGCAGATGCCATCTATGATATGATTGGTTTCCCTGATTTCATCCTGGAGCCCA AAGAGCTGGATGATGTTTATGATGGGTATGAAGTCTCTGAAGATTCTTTTTTCCAAAACATGTTGAATCT GTACAACTTCTCAGCTAAGGTGATGGCTGACCAGCTCCGCAAACCTCCCAGCCGAGACCAGTGGAGCATG ACACCTCAGACCGTGAACGCTTACTACCTTCCAACCAAGAATGAAATCGTCTTCCCTGCTGGCATCTTGC AGGCCCCCTTCTATGCTCACAACCATCCAAAGGCCTTGAACTTTGGTGGCATCGGCGTAGTGATGGGCCA TGAGTTGACACATGCCTTTGATGACCAAGGGCGTGAGTATGACAAAGAAGGGAATCTGCGTCCTTGGTGG CAGAATGAGTCACTGACGGCTTTCCAGAACCATACAGCCTGCATGGAAGAACAGTACAGCCAGTACCAGG TCAATGGAGAGAGGCTCAATGGACTCCAGACCCTGGGGGAAAACATCGCCGATAATGGGGGCCTTAAGGC TGCTTACAATGCTTACAAAGCATGGCTGAGAAAGCATGGGGAGGAGCAGCCGCTGCCTGCTGTGGGGCTC ACCAATCACCAGCTTTTCTTCGTGGGATTTGCTCAGGTGTGGTGCTCGGTCCGCACACCAGAGAGCTCTC ACGAGGGGCTGGTGACCGACCCCCACAGCCCTGCCCGTTTCCGAGTGCTGGGCACTCTCTCCAACTCCCG AGACTTCCTTCGGCACTTCGGCTGCCCTGTCGGCTCCCCCATGAACCCAGGGCAGCTATGTGAGGTGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219025 representing NM_177941
Red=Cloning site Green=Tags(s) MASPRTPVSPPELPEKNFQYRQVQYWDQRYKDAADSGPYEWFGDFASFRALLEPELCPEDRILVLGCGNS ALSYELFLGGFPNVTSVDYSPVVVAAMQVRYAHVPSLRWETMDVRALDFPSGSFDVVLEKGTLDAMLAGE PDPWNVSSEGVHTVDQVLSEMVEYKRAKLRDEESPEITVEGRATRDSLEVGFQKRTRQLFGSHTQLELVL AGLILVLAALLLGCLVALWVHRDPAHSTCVTEACIRVAGKILESLDRGVSPCQDFYQFSCGGWIRRNPLP NGRSRWNTFNSLWDQNQAILKHLLENTTFNSSSEAERKTRSFYLSCLQSERIEKLGAKPLRDLIDKIGGW NITGPWDEDSFMDVLKAVAGTYRATPFFTVYVSADSKSSNSNIIQVDQSGLFLPSRDYYLNRTANEKVLT AYLDYMVELGVLLGGQPTSTREQMQQVLELEIQLANITVPQDQRRDEEKIYHKMSISELQALAPAVDWLE FLSFLLSPLELGDSEPVVVYGTEYLQQVSELINRTEPSILNNYLIWNLVQKTTSSLDQRFETAQEKLLET LYGTKKSCTPRWQTCISNTDDALGFALGSLFVKATFDRQSKEIAEGMINEIRSAFEETLGDLVWMDEKTR LAAKEKADAIYDMIGFPDFILEPKELDDVYDGYEVSEDSFFQNMLNLYNFSAKVMADQLRKPPSRDQWSM TPQTVNAYYLPTKNEIVFPAGILQAPFYAHNHPKALNFGGIGVVMGHELTHAFDDQGREYDKEGNLRPWW QNESLTAFQNHTACMEEQYSQYQVNGERLNGLQTLGENIADNGGLKAAYNAYKAWLRKHGEEQPLPAVGL TNHQLFFVGFAQVWCSVRTPESSHEGLVTDPHSPARFRVLGTLSNSRDFLRHFGCPVGSPMNPGQLCEVW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177941 |
ORF Size | 2730 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177941.1, NP_808810.1 |
RefSeq Size | 3436 bp |
RefSeq ORF | 2733 bp |
Locus ID | 110599584 |
UniProt ID | P0DPE0 |
Cytogenetics | 16 B1 |
Gene Summary | This locus represents naturally occurring readthrough transcription between the adjacent genes eukaryotic translation elongation factor 1 alpha lysine specific methyltransferase 4 (GeneID:110599566) and endothelin converting enzyme 2 (GeneID:107522). The readthrough transcript representing this gene encodes a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Jul 2017] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222543 | Ece2 (untagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 1, (10ug) |
USD 917.00 |
|
MR219025 | Ece2 (Myc-DDK-tagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 1 |
USD 833.00 |
|
MR219025L3 | Lenti ORF clone of Ece2 (Myc-DDK-tagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 1 |
USD 1,133.00 |
|
MR219025L4 | Lenti ORF clone of Ece2 (mGFP-tagged) - Mouse endothelin converting enzyme 2 (Ece2), transcript variant 1 |
USD 1,133.00 |
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