Grid1 (NM_008166) Mouse Tagged ORF Clone

CAT#: MG219008

Grid1 (tGFP-tagged) - Mouse glutamate receptor ionotropic delta 1 (Grid1), (10ug)

Product Images

Circular map for MG219008

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Grid1
• Synonyms: GluD1; GluRdelta1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG219008 representing NM_008166
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGCGTTGACGCTGTGGCTTCTTCCCTGGATATGCCAGTGCGTTACGGTGCGGGCCGACTCCATCA
  TCCACATCGGTGCCATCTTCGAGGAGAACGCAGCCAAGGACGACAGGGTGTTCCAGTTGGCTGTATCAGA
  CCTGAGCCTCAATGATGACATCCTGCAGAGTGAGAAGATCACCTACTCCATCAAGGTCATCGAGGCCAAT
  AATCCGTTCCAGGCGGTGCAAGAAGCCTGTGACCTCATGACCCAGGGAATTTTGGCCTTGGTCACATCCA
  CGGGCTGTGCATCCGCCAATGCCCTGCAGTCCCTCACAGACGCCATGCACATCCCACACCTCTTTGTCCA
  GCGCAACCCCGGGGGTTCACCACGTACTGCCTGCCACCTGAACCCTAGCCCTGACGGCGAGGCCTACACA
  CTGGCTTCGAGACCACCTGTCCGTCTCAATGATGTCATGCTCAGGCTGGTGACAGAACTGCGCTGGCAGA
  AGTTCGTCATGTTCTATGACAGCGAGTATGATATCCGTGGGCTCCAAAGCTTCCTGGATCAGGCCTCAAG
  GCTGGGTCTGGATGTCTCTTTACAAAAGGTGGACAAGAACATCAGTCACGTGTTCACTAGCCTTTTCACC
  ACCATGAAGACCGAGGAGCTGAATCGCTACCGGGACACACTGCGCCGTGCCATTCTACTGCTCAGCCCAC
  AGGGGGCGCACTCCTTCATCAATGAGGCTGTGGAGACCAACTTGGCTTCCAAGGACAGCCACTGGGTCTT
  TGTGAATGAGGAAATCAGTGACCCCGAGATCCTGGATCTGGTCCACAGTGCCCTTGGCAGGATGACCGTG
  GTCCGGCAAATCTTCCCATCTGCAAAGGACAACCAGAAATGTATGAGGAATAACCACCGCATCTCCTCCC
  TGCTCTGTGATCCACAGGAAGGCTACCTCCAAATGCTGCAGATCTCCAATCTCTACCTGTATGACAGTGT
  TCTGATGCTGGCCAACGCCTTCCACAGGAAGCTGGAAGACCGGAAGTGGCATAGTATGGCAAGCCTTAAC
  TGCATCCGGAAGTCTACCAAGCCATGGAATGGAGGGAGATCCATGCTAGACACGATTAAAAAGGGACATA
  TCACTGGCCTCACAGGGGTTATGGAGTTTCGGGAAGATAGCTCAAATCCTTATGTCCAGTTTGAAATCCT
  TGGCACAACCTATAGTGAGACTTTTGGCAAAGACATGCGCAAGCTGGCCACCTGGGACTCGGAGAAGGGC
  TTGAATGGCAGTCTACAGGAGAGGCCCATGGGCAGCCGCCTCCAAGGACTGACTCTCAAAGTGGTGACTG
  TCTTGGAAGAGCCTTTTGTGATGGTAGCTGAGAATATCCTTGGACAACCCAAGCGTTACAAAGGGTTCTC
  CATAGATGTGCTGGATGCACTGGCTAAGGCTCTAGGATTCAAATATGAGATATACCAGGCCCCTGATGGC
  AGGTATGGTCACCAGCTCCATAACACCTCCTGGAATGGGATGATCGGGGAGCTCATTAGCAAGAGAGCAG
  ACTTGGCCATCTCTGCTATTACCATCACCCCAGAGAGAGAGAGCGTTGTGGACTTCAGCAAGCGCTACAT
  GGACTACTCAGTGGGGATCCTCATCAAGAAGCCTGAGGAGAAGATCAGCATCTTTTCCCTTTTCGCCCCC
  TTTGACTTTGCGGTGTGGGCCTGCATTGCCGCAGCCATTCCTGTGGTGGGCGTGCTCATATTCGTGTTGA
  ATCGGATACAGGCTGTAAGATCTCAGAGTGCCACCCAGCCACGACCCTCGGCTTCTGCCACCCTGCACAG
  TGCCATTTGGATTGTCTATGGAGCCTTTGTCCAGCAAGGTGGTGAGTCTTCGGTGAACTCTGTGGCCATG
  CGCATCGTGATGGGCAGCTGGTGGCTCTTCACCCTCATTGTGTGTTCCTCCTACACAGCCAACCTGGCCG
  CTTTCCTCACAGTGTCTAGGATGGACAACCCCATAAGGACATTTCAGGACCTGTCCAAGCAACTGGAGAT
  GTCTTATGGCACTGTCCGGGACTCTGCTGTCTATGAGTACTTCAGAGCCAAGGGGACCAATCCCCTGGAG
  CAGGATAGCACTTTTGCTGAGCTCTGGCGGACCATAAGCAAGAATGGAGGGGCTGACAACTGTGTGTCCA
  ATCCTTCAGAAGGTATCAGGAAGGCAAAGAAGGGGAACTACGCCTTTCTATGGGATGTGGCCGTGGTGGA
  GTATGCAGCCCTGACAGATGACGACTGCTCAGTGACTGTCATCGGCAACAGCATCAGCAGCAAGGGCTAC
  GGGATTGCCCTGCAACATGGCAGCCCCTACAGGGACCTTTTCTCCCAGAGGATCCTGGAGCTGCAAGACA
  CAGGGGACCTGGATGTGCTCAAGCAGAAGTGGTGGCCACACACAGGTCGATGTGACCTCACCAGCCATTC
  CAGTACCCAGACTGAAGGCAAATCCCTTAAGCTGCACAGCTTCGCTGGGGTCTTCTGCATTTTGGCCATT
  GGCCTCCTTCTTGCCTGCCTCGTGGCTGCTCTAGAGTTATGGTGGAACAGCAACCGGTGCCACCAGGAGA
  CCCCCAAAGAGGACAAAGAAGTGAATCTGGAGCAGGTACACCGGCGCATCAACAGCCTCATGGATGAAGA
  CATTGCTCACAAGCAAATTTCCCCAGCGTCCATTGAGCTTTCTGCCCTAGAGATGGGGGGCCTGGCTCCA
  AGCCAAGCTTTGGAGCCCACGAGGGAGTACCAGAACACCCAGCTCTCAGTCAGCACCTTTCTGCCGGAGC
  AGAGCAGCCATGGCACCAGCCGGACACTGTCGTCAGGGCCCAGCAGCAACCTGCCACTGCCACTGAGCAG
  CTCAGCCACTATGCCCTCAATTCAGTGCAAACACAGGTCGCCCAATGGGGGACTGTTTCGACAGAGCCCG
  GTGAAGACCCCCATCCCTATGTCCTTCCAGCCCGTGCCTGGAGGCGTCCTTCCAGAGGCTCTGGACACCT
  CTCATGGCACCTCCATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG219008 representing NM_008166
  Red=Cloning site Green=Tags(s)
  
  MEALTLWLLPWICQCVTVRADSIIHIGAIFEENAAKDDRVFQLAVSDLSLNDDILQSEKITYSIKVIEAN
  NPFQAVQEACDLMTQGILALVTSTGCASANALQSLTDAMHIPHLFVQRNPGGSPRTACHLNPSPDGEAYT
  LASRPPVRLNDVMLRLVTELRWQKFVMFYDSEYDIRGLQSFLDQASRLGLDVSLQKVDKNISHVFTSLFT
  TMKTEELNRYRDTLRRAILLLSPQGAHSFINEAVETNLASKDSHWVFVNEEISDPEILDLVHSALGRMTV
  VRQIFPSAKDNQKCMRNNHRISSLLCDPQEGYLQMLQISNLYLYDSVLMLANAFHRKLEDRKWHSMASLN
  CIRKSTKPWNGGRSMLDTIKKGHITGLTGVMEFREDSSNPYVQFEILGTTYSETFGKDMRKLATWDSEKG
  LNGSLQERPMGSRLQGLTLKVVTVLEEPFVMVAENILGQPKRYKGFSIDVLDALAKALGFKYEIYQAPDG
  RYGHQLHNTSWNGMIGELISKRADLAISAITITPERESVVDFSKRYMDYSVGILIKKPEEKISIFSLFAP
  FDFAVWACIAAAIPVVGVLIFVLNRIQAVRSQSATQPRPSASATLHSAIWIVYGAFVQQGGESSVNSVAM
  RIVMGSWWLFTLIVCSSYTANLAAFLTVSRMDNPIRTFQDLSKQLEMSYGTVRDSAVYEYFRAKGTNPLE
  QDSTFAELWRTISKNGGADNCVSNPSEGIRKAKKGNYAFLWDVAVVEYAALTDDDCSVTVIGNSISSKGY
  GIALQHGSPYRDLFSQRILELQDTGDLDVLKQKWWPHTGRCDLTSHSSTQTEGKSLKLHSFAGVFCILAI
  GLLLACLVAALELWWNSNRCHQETPKEDKEVNLEQVHRRINSLMDEDIAHKQISPASIELSALEMGGLAP
  SQALEPTREYQNTQLSVSTFLPEQSSHGTSRTLSSGPSSNLPLPLSSSATMPSIQCKHRSPNGGLFRQSP
  VKTPIPMSFQPVPGGVLPEALDTSHGTSI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008166
• ORF Size: 3027 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008166.2, NP_032192.2
• RefSeq Size: 3445 bp
• RefSeq ORF: 3030 bp
• Locus ID: 14803
• UniProt ID: Q61627
• Cytogenetics: 14 20.84 cM
• Gene Summary: Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists.[UniProtKB/Swiss-Prot Function]