Mcu (NM_001033259) Mouse Tagged ORF Clone

CAT#: MG218926

Mcu (tGFP-tagged) - Mouse coiled-coil domain containing 109A (Ccdc109a), (10ug)

Product Images

Circular map for MG218926

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mcu
• Synonyms: 2010012O16Rik; AV064928; C10orf42; Ccdc109a; D130073L02Rik; Gm64
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218926 representing NM_001033259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGCCGCAGGTAGATCGCTCCTGCTGCTGCTCTGCTCCCGGGGCGGCGGCGGGGGCGCCGGCG
  GCTGCGGAGCGCTGACTGCCGGCTGCTTTCCCGGGCTGGGCGTCAGCCGCCACCGGCCGCACCAGCAGCA
  CCGGACGGCACACCAGAGGCCGGCTTCCTGGCAGAGCGTGGGAGCCGCATATTGCAGTACGGTTGTGCCC
  TCTGATGACGTGACGGTGGTTTACCAAAATGGATTACCGGTGATATCTGTGAGGCTACCCTCTCGACGCG
  AGCGCTGCCAATTCACACTCAAGCCTATCTCTGACTCAGTCGGCGTGTTTTTACGACAACTGCAAGAGGA
  GGATCGGGGAATCGACCGGGTCGCCATCTACTCACCAGATGGCGTTCGAGTCGCTGCCTCCACGGGGATA
  GACCTCCTGCTCCTCGATGACTTTAAGCTTGTCATTAATGACTTAACATACCACGTACGGCCACCAAAGA
  GAGACCTCCTAAGCCATGAAGATGCAGCGACGCTGAACGACGTGAAGACCCTGGTCCAGCAGCTGTACAC
  CACACTGTGCATTGAGCAGCATCAGCTTAACAAAGAGCGGGAGCTCGTGGAGAGGTTAGAGGACCTCAAG
  CAGCAGCTGGCCCCCCTGGAGAAGGTACGAATTGAAATTAGCAGAAAAGCAGAGAAGAGGACCACTCTGG
  TGCTGTGGGGAGGCCTGGCCTACATGGCCACCCAGTTTGGCATTCTGGCCCGGCTCACCTGGTGGGAGTA
  CTCGTGGGACATCATGGAGCCCGTCACCTACTTCATCACGTACGGAAGCGCCATGGCCATGTATGCGTAT
  TTTGTAATGACGCGCCAGGAATATGTTTATCCAGAAGCCAGAGACAGACAATACTTATTATTTTTCCATA
  AAGGAGCCAAAAAGTCACGTTTCGACCTAGAGAAATACAATCAGCTCAAGGATGCAATTGCTCAGGCAGA
  AATGGATCTTAAGAGACTGAGAGACCCATTACAAGTACACCTGCCCCTCCGACAGATCGGAGAAAAGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218926 representing NM_001033259
  Red=Cloning site Green=Tags(s)
  
  MAAAAGRSLLLLLCSRGGGGGAGGCGALTAGCFPGLGVSRHRPHQQHRTAHQRPASWQSVGAAYCSTVVP
  SDDVTVVYQNGLPVISVRLPSRRERCQFTLKPISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGI
  DLLLLDDFKLVINDLTYHVRPPKRDLLSHEDAATLNDVKTLVQQLYTTLCIEQHQLNKERELVERLEDLK
  QQLAPLEKVRIEISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMAMYAY
  FVMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLRDPLQVHLPLRQIGEKE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001033259
• ORF Size: 1050 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001033259.4
• RefSeq Size: 1423 bp
• RefSeq ORF: 1053 bp
• Locus ID: 215999
• UniProt ID: Q3UMR5
• Cytogenetics: 10 B4
• Gene Summary: Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria (PubMed:21685886, PubMed:23900286, PubMed:24212091). Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex) (By similarity). Activity is regulated by MICU1 and MICU2 (By similarity). At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity (By similarity). Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways (By similarity). Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes (By similarity). While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells (PubMed:26119742, PubMed:26119731, PubMed:25603276). Participates in mitochondrial permeability transition during ischemia-reperfusion injury (PubMed:26119731). Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake (By similarity). Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults (PubMed:25732818). Regulates synaptic vesicle endocytosis kinetics in central nerve terminal (PubMed:26644474). Involved in antigen processing and presentation (PubMed:25251370).[UniProtKB/Swiss-Prot Function]