Cadm2 (NM_178721) Mouse Tagged ORF Clone

CAT#: MG218922

Cadm2 (tGFP-tagged) - Mouse cell adhesion molecule 2 (Cadm2) transcript variant 1, (10ug)

Product Images

Circular map for MG218922

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cadm2
• Synonyms: 2900078E11Rik; 9330131D06; A830029E02Rik; Igdf4d; Igsf4d; NECL3; SynCAM 2; SynCAM2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218922 representing NM_178721
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATTTGGAAACGCAGCGCGGTTCTCCGCTTCTACAGTGTCTGCGGGCTCCTGTTACAAGCGGCTGCTT
  CAAAAAGTAAAGTCAAAGGCAGCCAAGGGCAGTTTCCACTAACTCAGAATGTCACGGTTGTTGAAGGTGG
  AACTGCAATTTTGACCTGCAGAGTTGATCAAAATGATAACACCTCCCTCCAGTGGTCAAATCCAGCTCAA
  CAGACTCTGTACTTCGATGACAAGAAAGCTTTAAGGGACAATCGGATCGAGCTGGTTCGCGCTTCTTGGC
  ATGAGTTGAGCATCAGTGTCAGTGATGTGTCTCTCTCGGATGAAGGACAGTACACCTGCTCCTTATTTAC
  AATGCCTGTCAAAACTTCCAAGGCCTATCTCACTGTCCTGGGTGTTCCGGAGAAGCCTCAGATTAGTGGA
  TTTTCGTCACCCGTCATGGAGGGAGACCTGATGCAGCTGACTTGTAAGACGTCCGGCAGTAAGCCTGCGG
  CTGATATAAGATGGTTCAAAAATGACAAAGAAATCAAAGATGTGAAGTACTTGAAAGAGGAGGATGCTAA
  CCGAAAGACCTTCACCGTTAGCAGCACGCTGGATTTCCGAGTGGACCGTAGCGATGATGGAGTGGCGGTC
  ATCTGCAGAGTAGACCATGAGTCCCTCAATGCCACCCCTCAGGTAGCCATGCAGGTGCTAGAAATTCACT
  ATACACCATCAGTTAAGATTATACCATCGACTCCTTTTCCACAAGAAGGGCAGGCTTTAACGTTGACCTG
  TGAATCTAAAGGAAAACCACTGCCAGAACCTGTTTTGTGGACGAAGGATGGTGCCGAATTACCAGATCCT
  GATCGAATGGTTGTGAGTGGTAGGGAACTCAACATTCTCTTCCTGAACAAAACGGACAATGGCACTTACC
  GATGTGAAGCCACTAACACCATTGGCCAAAGCAGTGCCGAGTATGTCCTCATTGTTCACGATCCCAATTC
  TCTGGCTGGCCAGAATGGCCCTGACCATGCTCTCATCGGAGGAATAGTGGCTGTTGTTGTATTTGTTACA
  CTGTGCTCCATATTCCTGCTTGGCCGATATCTGGCAAGACATAAAGGAACATATCTAACAAATGAAGCTA
  AAGGAGCCGAAGATGCACCAGATGCCGACACAGCCATTATCAATGCTGAAGGCAGCCAAGTCAATGCCGA
  AGAGAAAAAAGAGTATTTCATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218922 representing NM_178721
  Red=Cloning site Green=Tags(s)
  
  MIWKRSAVLRFYSVCGLLLQAAASKSKVKGSQGQFPLTQNVTVVEGGTAILTCRVDQNDNTSLQWSNPAQ
  QTLYFDDKKALRDNRIELVRASWHELSISVSDVSLSDEGQYTCSLFTMPVKTSKAYLTVLGVPEKPQISG
  FSSPVMEGDLMQLTCKTSGSKPAADIRWFKNDKEIKDVKYLKEEDANRKTFTVSSTLDFRVDRSDDGVAV
  ICRVDHESLNATPQVAMQVLEIHYTPSVKIIPSTPFPQEGQALTLTCESKGKPLPEPVLWTKDGAELPDP
  DRMVVSGRELNILFLNKTDNGTYRCEATNTIGQSSAEYVLIVHDPNSLAGQNGPDHALIGGIVAVVVFVT
  LCSIFLLGRYLARHKGTYLTNEAKGAEDAPDADTAIINAEGSQVNAEEKKEYFI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178721
• ORF Size: 1212 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178721.4, NP_848836.2
• RefSeq Size: 9580 bp
• RefSeq ORF: 1215 bp
• Locus ID: 239857
• UniProt ID: Q8BLQ9
• Cytogenetics: 16 C1.3- C2
• Gene Summary: Adhesion molecule that engages in homo- and heterophilic interactions with the other nectin-like family members, leading to cell aggregation. Important for synapse organization, providing regulated trans-synaptic adhesion. Preferentially binds to oligodendrocytes (By similarity).[UniProtKB/Swiss-Prot Function]