Nlrp5 (NM_001039143) Mouse Tagged ORF Clone

CAT#: MG218785

Nlrp5 (tGFP-tagged) - Mouse NLR family pyrin domain containing 5 (Nlrp5) transcript variant 2, (10ug)

Product Images

Circular map for MG218785

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nlrp5
• Synonyms: Mat; Mater; N; Nalp5; O; Op1; PAN11
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218785 representing NM_001039143
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGTCCTCCAGAAAAAGAAAGTAAAGCAATCTTGAAAGCACGTGGATTGGAAGAGGAACAGAAGTCAG
  AAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCAGAAAGACCAAGGACCAGAGCAGGAGCA
  GACATCAGAAAGCACAATGGGTCCTCCAGAAAAAGACAGTAAAGCAATCTTGAAAGCACGTGGATTGGAA
  GAGGAACAGAAGTCAGAAAGCACAATGTCTCCTTCAGAAAATGTCAGTAGAGCAATCCTGAAAGACAGTG
  GATCAGAAGAAGTGGAACAGGCGTCAGAAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCA
  GAAAGACCAAGGACCAGAGCAGGAGCAGACATCAGATAATGGAGGTGACTTACAAGACTACAAGGCCCAT
  GTGATTGCTAAGTTCGACACAAGTGTGGATCTACACTATGACAGCCCAGAGATGAAATTATTGTCTGATG
  CTTTTAAACCATACCAGAAAACCTTCCAGCCTCACACCATTATCCTACATGGAAGACCAGGAGTTGGGAA
  GTCAGCTTTGGCCAGAAGTATTGTTCTTGGCTGGGCACAGGGTAAACTCTTCCAAAAAATGTCCTTTGTC
  ATCTTCTTCTCTGTTAGAGAAATAAAGTGGACAGAGAAGAGCAGTTTGGCACAGCTGATTGCTAAGGAGT
  GTCCAGACTCCTGGGATCTAGTGACAAAGATCATGTCCCAACCAGAAAGACTCTTGTTTGTCATAGATGG
  CTTGGATGATATGGACTCTGTCCTCCAACATGATGATATGACACTATCCAGAGACTGGAAGGATGAACAG
  CCCATATACATCCTGATGTACAGCCTCCTGAGGAAGGCTCTCCTACCTCAGTCCTTTCTCATCATTACCA
  CCAGAAACACAGGCTTAGAAAAACTCAAGTCAATGGTTGTGTCCCCCCTCTATATACTGGTTGAAGGACT
  GTCTGCATCAAGGAGATCTCAGCTGGTCCTCGAGAACATCTCCAATGAGTCTGATAGAATACAAGTCTTC
  CATTCTCTGATAGAAAATCACCAGCTGTTTGACCAATGCCAGGCCCCCTCTGTGTGCTCCCTGGTCTGTG
  AGGCTCTACAGCTACAGAAGAAACTGGGAAAGAGATGCACCCTACCCTGCCAGACTCTCACCGGTTTGTA
  TGCCACGTTGGTGTTTCACCAGCTCACCTTGAAAAGGCCTTCCCAGAGCGCTCTCAGTCAGGAAGAACAG
  ATTACTCTAGTGGGTTTGTGCATGATGGCAGCTGAAGGAGTGTGGACCATGAGGTCGGTGTTCTATGATG
  ATGACCTGAAGAACTATAGCCTAAAGGAGTCTGAGATCTTGGCCCTCTTTCACATGAACATCCTTCTCCA
  GGTTGGCCACAACAGTGAGCAGTGTTATGTTTTCTCCCACCTCAGCCTGCAGGATTTCTTTGCTGCCTTA
  TATTATGTTTTAGAAGGGCTGGAGGAATGGAATCAGCATTTTTGCTTCATTGAAAACCAAAGGAGCATCA
  TGGAGGTGAAGAGAACTGACGACACTCGCCTCCTCGGGATGAAGCGTTTCTTATTTGGCCTCATGAACAA
  GGATATCTTGAAGACTCTGGAGGTTCTGTTTGAATATCCCGTGATTCCAACTGTTGAGCAGAAGCTCCAA
  CACTGGGTCTCTCTGATAGCTCAGCAGGTCAATGGCACCAGCCCAATGGACACCCTGGATGCCTTCTATT
  GTCTATTTGAGTCTCAGGATGAAGAGTTTGTTGGCGGGGCTCTCAAACGCTTCCAAGAAGTGTGGCTGCT
  GATTAACCAGAAGATGGACTTGAAGGTCTCTTCCTACTGTCTCAAGCACTGTCAGAACTTGAAGGCAATC
  CGGGTGGATATCAGAGACCTCCTCTCGGTAGATAATACTCTCGAGCTGTGCCCTGTTGTTACTGTCCAGG
  AGACACAATGTAAGCCCCTCCTCATGGAGTGGTGGGGAAACTTCTGCTCTGTGCTTGGCAGCCTCCGGAA
  CTTGAAGGAGCTGGACTTGGGCGACAGCATCCTGAGTCAACGGGCCATGAAGATACTGTGCCTCGAGCTG
  CGGAATCAGTCCTGCAGAATACAGAAGCTGACGTTTAAGAGTGCAGAGGTAGTGTCTGGCCTGAAACATC
  TCTGGAAGCTCCTTTTTAGCAATCAAAACTTAAAGTACCTCAATCTAGGGAACACTCCCATGAAGGATGA
  TGACATGAAGTTAGCCTGCGAAGCGCTGAAACATCCAAAGTGCTCCGTGGAGACTCTGAGGTTGGATTCC
  TGTGAGTTAACCATCATTGGTTATGAGATGATCTCCACGCTTCTTATTTCAACCACCAGGCTAAAGTGTC
  TCAGCCTGGCCAAAAATAGAGTGGGAGTAAAAAGCATGATATCCCTTGGGAATGCCTTGAGTAGCTCAAT
  GTGTCTACTGCAAAAGTTGATACTGGACAACTGTGGCCTCACACCTGCCAGCTGCCACCTTCTGGTCTCA
  GCCCTTTTCAGCAACCAGAACTTGACACACCTGTGCCTGTCAAACAACAGCCTGGGGACTGAAGGAGTGC
  AACAGCTGTGTCAGTTCCTGAGGAATCCAGAATGTGCTCTCCAGCGGCTGATACTGAATCACTGCAACAT
  TGTAGATGATGCTTATGGCTTCCTGGCAATGAGACTTGCAAACAACACAAAGCTGACCCACCTGAGCCTG
  ACCATGAACCCCGTAGGGGATGGTGCAATGAAGCTACTGTGTGAAGCTTTAAAGGAACCTACTTGTTACC
  TTCAAGAACTGGAACTAGTGGACTGCCAACTCACACAGAACTGCTGCGAGGACCTGGCCTGTATGATCAC
  AACAACCAAGCACTTAAAAAGTTTGGATCTTGGTAACAACGCCCTGGGTGACAAAGGAGTCATAACCCTG
  TGTGAGGGACTGAAGCAAAGTAGCAGCTCCCTGAGGAGACTTGGGTTGGGGGCATGTAAGTTGACTTCCA
  ATTGCTGTGAGGCATTGTCATTGGCCATCTCTTGCAACCCTCACCTGAACAGCCTAAACCTGGTGAAGAA
  TGACTTCAGTACATCGGGGATGTTGAAGCTGTGCTCTGCGTTCCAATGCCCTGTCTCTAACCTGGGGATA
  ATTGGCCTGTGGAAGCAGGAGTACTATGCCCGAGTGAGAAGACAGCTGGAGGAAGTTGAGTTTGTCAAGC
  CCCACGTGGTGATTGATGGTGATTGGTATGCTAGTGATGAAGATGACCGAAACTGGTGGAAAAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218785 representing NM_001039143
  Red=Cloning site Green=Tags(s)
  
  MGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKDSKAILKARGLE
  EEQKSESTMSPSENVSRAILKDSGSEEVEQASERKMTSPENDSKSIQKDQGPEQEQTSDNGGDLQDYKAH
  VIAKFDTSVDLHYDSPEMKLLSDAFKPYQKTFQPHTIILHGRPGVGKSALARSIVLGWAQGKLFQKMSFV
  IFFSVREIKWTEKSSLAQLIAKECPDSWDLVTKIMSQPERLLFVIDGLDDMDSVLQHDDMTLSRDWKDEQ
  PIYILMYSLLRKALLPQSFLIITTRNTGLEKLKSMVVSPLYILVEGLSASRRSQLVLENISNESDRIQVF
  HSLIENHQLFDQCQAPSVCSLVCEALQLQKKLGKRCTLPCQTLTGLYATLVFHQLTLKRPSQSALSQEEQ
  ITLVGLCMMAAEGVWTMRSVFYDDDLKNYSLKESEILALFHMNILLQVGHNSEQCYVFSHLSLQDFFAAL
  YYVLEGLEEWNQHFCFIENQRSIMEVKRTDDTRLLGMKRFLFGLMNKDILKTLEVLFEYPVIPTVEQKLQ
  HWVSLIAQQVNGTSPMDTLDAFYCLFESQDEEFVGGALKRFQEVWLLINQKMDLKVSSYCLKHCQNLKAI
  RVDIRDLLSVDNTLELCPVVTVQETQCKPLLMEWWGNFCSVLGSLRNLKELDLGDSILSQRAMKILCLEL
  RNQSCRIQKLTFKSAEVVSGLKHLWKLLFSNQNLKYLNLGNTPMKDDDMKLACEALKHPKCSVETLRLDS
  CELTIIGYEMISTLLISTTRLKCLSLAKNRVGVKSMISLGNALSSSMCLLQKLILDNCGLTPASCHLLVS
  ALFSNQNLTHLCLSNNSLGTEGVQQLCQFLRNPECALQRLILNHCNIVDDAYGFLAMRLANNTKLTHLSL
  TMNPVGDGAMKLLCEALKEPTCYLQELELVDCQLTQNCCEDLACMITTTKHLKSLDLGNNALGDKGVITL
  CEGLKQSSSSLRRLGLGACKLTSNCCEALSLAISCNPHLNSLNLVKNDFSTSGMLKLCSAFQCPVSNLGI
  IGLWKQEYYARVRRQLEEVEFVKPHVVIDGDWYASDEDDRNWWKN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001039143
• ORF Size: 3285 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039143.2, NP_001034232.1
• RefSeq Size: 3463 bp
• RefSeq ORF: 3288 bp
• Locus ID: 23968
• UniProt ID: Q9R1M5
• Cytogenetics: 7 10.22 cM
• Gene Summary: This gene encodes a member of the NACHT, leucine-rich repeat, and pyrin domain containing family. Members of this family have a pyrin domain at the N-terminus, a central NACHT domain, and a C-terminal leucine-rich repeat domain. This gene encodes a maternal-effect factor that is essential for early embryonic development in the mouse. Homozygous null mutant females are sterile, and embryos die following the first cleavage. This gene is required for endoplasmic reticulum redistribution and calcium homeostasis in oocytes. In addition, ovulated oocytes mutant for this gene have abnormal mitochondrial localization and increased mitochondrial activity, which results in mitochondrial damage and early embryonic lethality. Pseudogenes of this gene have been found on chromosomes 7 and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]