Fut1 (NM_008051) Mouse Tagged ORF Clone

CAT#: MG218449

Fut1 (tGFP-tagged) - Mouse fucosyltransferase 1 (Fut1), (10ug)

Product Images

Circular map for MG218449

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fut1
• Synonyms: MFUT-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218449 representing NM_008051
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACTCCCAGCCGGAGGCAGCTCTGCCTGGCATTTCTGTTGGTCTGTGTGCTCTCTGCCGGCTCCT
  TCTTTTTCCACCTGAATGGAGGAAACTTCTTTCGAAATGGTCTAACTCTCTCTGTCCTGTGTTCAGACTA
  TCACCTGCTGAAGTCCCCAGTGGCCATGGTATGCCTACCTCATCCATTGCAGACATCTAATGGCTCCCCT
  TCCTGTCCTGAGCAGTCCTCCTCACTCTCTGGGACTTGGACAATCACCCCAGGAGGCAGGTTTGGTAACC
  AGATGGGGCAGTATGCTACATTGCTGGCCCTAGCCCAGCTCAATGGTCGCCAAGCCTTCATCCAACCTGA
  GATGCATGCCGCCCTGGCCCCCGTGTTCCGAATCTCCCTGCCAGTGCTGGACCCTGAGGTGGACAGCCTC
  ACACCTTGGCAGCACTTAGTCCTACATGACTGGATGTCAGAGGAGTACTCCCATCTGGAGGACCCATTTC
  TCAAGCTGTCTGGTTTCCCCTGCTCTTGGACCTTTTTCCATCATCTTCGGGAACAGATTCGTAGGGAATT
  CACTCTGCATAACCATCTACGGGAAGGTGCCCAGTACCTGTTGAGCGGGCTCCGTATAGGCCCGGCGGGC
  ATCCGCCCTCATACCTTTGTGGGTGTCCATGTGCGTCGTGGAGACTATCTGGAGGTGATGCCCAATCGCT
  GGAAGGGTGTGGTGGGTGACCGAGCTTACCTCCAGCAAGCCATGGACTGGTTCCGGGCCCGACACAAAGA
  CCCCATCTTTGTGGTCACCAGCAATGGCATGAAATGGTGTTTGGAGAACATTGACACATCCCATGGTGAT
  GTGGTCTTCGCTGGCAATGGACAGGAGGGTACACCGGGGAAGGACTTTGCACTTCTCACACAGTGTAACC
  ACACCATCATGACTATTGGCACCTTTGGCTTCTGGGCTGCCTACTTAGCTGGTGGAGACACGGTCTACCT
  TGCAAACTTCACCCTGCCAGATTCGGAGTTTCTGAAGATCTTCAGGCCTGAGGCTGCCTTCCTGCCTGAG
  TGGGTGGGCATCAATGCAGACTTGTCCCCGCTGCAGGCTCAATTTGACCCCTGGAAGCCAGACAGTCTTT
  TTAGATTGGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218449 representing NM_008051
  Red=Cloning site Green=Tags(s)
  
  MWTPSRRQLCLAFLLVCVLSAGSFFFHLNGGNFFRNGLTLSVLCSDYHLLKSPVAMVCLPHPLQTSNGSP
  SCPEQSSSLSGTWTITPGGRFGNQMGQYATLLALAQLNGRQAFIQPEMHAALAPVFRISLPVLDPEVDSL
  TPWQHLVLHDWMSEEYSHLEDPFLKLSGFPCSWTFFHHLREQIRREFTLHNHLREGAQYLLSGLRIGPAG
  IRPHTFVGVHVRRGDYLEVMPNRWKGVVGDRAYLQQAMDWFRARHKDPIFVVTSNGMKWCLENIDTSHGD
  VVFAGNGQEGTPGKDFALLTQCNHTIMTIGTFGFWAAYLAGGDTVYLANFTLPDSEFLKIFRPEAAFLPE
  WVGINADLSPLQAQFDPWKPDSLFRLV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008051
• ORF Size: 1131 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008051.6, NP_032077.2
• RefSeq Size: 2578 bp
• RefSeq ORF: 1134 bp
• Locus ID: 14343
• UniProt ID: O09160
• Cytogenetics: 7 29.39 cM
• Gene Summary: This gene is one of three genes in mouse which encode a galactoside 2-L-fucosyltransferase. These genes differ in their developmental- and tissue-specific expression. The encoded type II membrane protein is anchored in the Golgi apparatus and controls the final step in the creation of alpha (1,2) fucosylated carbhohydrates by the addition of a terminal fucose in an alpha (1,2) linkage. This enzyme is required for the synthesis of the Lewis antigen as well as the H-antigen, a precursor of the A and B antigens of the ABH histo-blood group. The biological function of the fucosylated carbhohydrate products is thought to involve cell-adhesion and interactions with microorganisms. Disruption of this gene impairs development of the olfactory nerve and maturation of the glomerular layer of the main olfactory bulb. Alternative splicing results in multiple transcript variants which encode distinct isoforms. [provided by RefSeq, Dec 2012]