Atrn (NM_009730) Mouse Tagged ORF Clone

CAT#: MG218405

Atrn (tGFP-tagged) - Mouse attractin (Atrn), (10ug)

Product Images

Circular map for MG218405

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Atrn
• Synonyms: AW558010; mg; Mgc; Mgca
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218405 representing NM_009730
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGGCGGTGGCGGCGGCGGCGGCGACTGAGGCGCGGCTGAGGGGGAGCACGACGGCGACAGCAGCGC
  CTGCGGGCAGGAAGGGCAGGCAGCACCGACCCTGCACCGCGACAGGGGCCTGGAGGCCGGGACCGCGCGC
  CCGGCTGTGTCTACCGCGGGTGCTGTCGCGGGCGCTGCCCCCGCCGCCGCTGCTGCCGCTGCTCTTTTCG
  CTGCTGCTGCTGCCGCTGCCCCGGGAGGCCGAGGCCGCTGCGGTGGCGGCGGCGGTGTCGGGCTCGGCCG
  CAGCCGAGGCCAAGGAATGTGACCGGCCGTGTGTCAACGGCGGCCGCTGCAACCCTGGCACCGGCCAGTG
  CGTCTGCCCCACGGGCTGGGTGGGCGAGCAATGCCAGCACTGCGGGGGCCGCTTCAGACTAACTGGCTCT
  TCTGGATTTGTAACAGATGGACCTGGGAATTATAAATATAAGACGAAGTGCACATGGCTCATTGAAGGAC
  AGCCAAATAGAATAATGAGACTTCGCTTCAACCATTTTGCTACAGAATGTAGCTGGGACCATTTATATGT
  TTATGATGGGGACTCAATCTACGCACCTCTGATTGCTGCCTTTAGTGGCCTCATTGTTCCTGAAAGAGAT
  GGCAATGAGACGGCTCCTGAGGTCACTGTCACTTCAGGTTATGCACTGCTGCATTTTTTCAGTGATGCTG
  CTTATAATCTGACTGGATTTAATATCACTTACAATTTTGACATGTGTCCGAATAATTGCTCAGGCCGAGG
  AGAGTGTAAGAGCAGTAACAGCAGCAGCGCTGTTGAGTGTGAATGTTCTGAAAACTGGAAAGGGGAGTCG
  TGTGACATTCCTCACTGTACAGACAACTGTGGCTTTCCTCACCGAGGCATCTGTAATGCAAGCGATACCA
  GAGGGTGCTCCTGCTTTCCTCACTGGCAGGGTCCTGGATGCTCAATTCCTGTGCCAGCTAACCAGTCTTT
  TTGGACTCGAGAAGAATATTCTGATTTAAAGCTTCCCAGAGCCTCTCATAAAGCTGTGGTCAATGGAAAT
  ATAATGTGGGTTGTTGGCGGATATATGTTCAACCATTCAGATTACAGCATGGTTTTAGCGTATGACCTGA
  CTTCTAGGGAATGGCTTCCACTAAACCATTCTGTGAACAGTGTGGTTGTAAGATATGGTCATTCTTTGGC
  ATTACATAAGGATAAAATCTACATGTATGGAGGAAAAATTGATTCAACAGGGAACGTGACCAATGAGCTG
  AGAGTATTTCATATTCATAATGAATCATGGGTATTGTTAACTCCGAAAGCTAAGGATCAGTATGCAGTGG
  TTGGACACTCAGCACACATTGTTACACTGGCATCTGGCCGTGTGGTCATGTTGGTCATCTTCGGTCATTG
  CCCACTCTATGGATATATAAGCGTTGTGCAGGAATATGACTTGGAAAAGAACACATGGAGTATATTACAT
  ACTCAGGGTGCTCTTGTGCAAGGGGGTTATGGCCACAGTAGTGTTTATGATGACAGGACCAAGGCTCTGT
  ACGTTCATGGTGGCTACAAGGCTTTCAGCGCCAACAAATACCGGCTTGCAGATGACCTCTACAGATACGA
  TGTGGATACTCAGATGTGGACCATTCTTAAGGACAGCCGATTTTTCCGTTACTTGCATACAGCTGTGATA
  GTGAGTGGAACCATGCTGGTGTTTGGAGGGAACACACACAATGACACTTCCATGAGCCACGGTGCCAAAT
  GCTTCTCCTCAGACTTCATGGCTTATGACATTGCTTGTGACCGATGGTCAGTGCTTCCCAGACCTGAGCT
  CCATCATGATGTCAACAGATTTGGCCATTCAGCAGTCTTGTACAACAGCACCATGTATGTGTTCGGCGGC
  TTCAACAGCCTCCTCCTCAGTGACGTCTTGGTCTTTACCTCGGAGCAGTGCGATGCACACCGCAGTGAAG
  CTGCTTGTGTGGCAGCAGGACCTGGTATCCGGTGTCTGTGGGACACACAGTCGTCTCGATGTACCTCCTG
  GGAGTTGGCAACTGAAGAACAAGCAGAAAAGTTAAAATCAGAGTGTTTTTCTAAAAGAACCCTTGACCAT
  GACAGATGTGACCAGCACACAGATTGTTACAGCTGCACAGCCAATACCAATGACTGCCACTGGTGCAATG
  ATCACTGTGTCCCTGTGAACCACAGCTGCACAGAAGGCCAGATCTCCATTGCCAAGTATGAGAGTTGCCC
  CAAGGATAACCCCATGTACTACTGCAATAAGAAAACCAGCTGCAGGAGCTGTGCCCTAGACCAGAACTGC
  CAGTGGGAGCCCCGGAATCAAGAGTGCATCGCCCTGCCGGAAAATATCTGTGGCAATGGCTGGCATTTGG
  TTGGAAACTCGTGTCTGAAAATCACTACTGCTAAGGAGAATTATGACAATGCTAAATTGTCCTGTAGGAA
  CCACAATGCCTTTTTGGCTTCCCTCACATCCCAGAAGAAGGTGGAGTTTGTCCTTAAGCAGCTTCGATTA
  ATGCAATCATCTCAAAGTATGTCCAAGCTCACTCTGACTCCATGGGTTGGTCTTCGGAAGATCAATGTGT
  CTTACTGGTGCTGGGAGGATATGTCTCCATTCACAAATAGTTTGCTGCAGTGGATGCCATCTGAGCCCAG
  TGATGCTGGCTTCTGTGGGATCTTGTCAGAGCCTAGTACTCGGGGATTAAAGGCTGCAACCTGCATCAAC
  CCTCTCAATGGCAGCGTCTGTGAAAGGCCTGCAAACCACAGTGCCAAGCAGTGCCGGACACCATGTGCCC
  TGCGGACAGCGTGTGGCGAGTGCACTAGCAGCAGCTCGGAGTGCATGTGGTGCAGTAACATGAAGCAGTG
  TGTGGACTCCAATGCCTACGTGGCCTCCTTCCCTTTTGGCCAGTGTATGGAATGGTATACGATGAGCAGC
  TGCCCACCTGAAAATTGCTCTGGCTACTGTACCTGCAGCCATTGCTTGGAGCAGCCAGGCTGTGGTTGGT
  GTACTGATCCTAGCAATACTGGGAAAGGAAAATGTATTGAGGGCAGCTATAAAGGACCTGTGAAGATGCC
  GTCACAGGCCTCTGCAGGAAATGTGTATCCACAGCCCCTTCTGAACTCCAGCATGTGTCTAGAGGACAGC
  AGATACAACTGGTCTTTCATTCACTGTCCAGCTTGCCAGTGCAACGGACACAGCAAATGCATCAACCAGA
  GTGTCTGTGAGAAGTGTGAGGACCTGACCACGGGCAAGCACTGCGAGACCTGCATATCTGGCTTCTATGG
  TGACCCGACTAATGGAGGCAAATGTCAGCCATGCAAGTGCAATGGGCACGCATCACTGTGCAACACCAAC
  ACCGGCAAGTGCTTCTGTACCACCAAAGGTGTCAAGGGGGACGAGTGCCAGCTATGTGAGGTAGAAAATC
  GATACCAGGGAAACCCTCTCAAAGGAACATGCTACTATACCCTTCTCATTGACTATCAGTTCACCTTTAG
  CCTGTCCCAGGAAGACGACCGCTACTACACAGCCATCAACTTTGTGGCTACTCCTGATGAACAAAACAGG
  GATTTGGACATGTTCATCAATGCCTCCAAAAACTTCAACCTCAACATCACCTGGGCCACCAGCTTCCCAG
  CCGGAACCCAGACTGGAGAAGAGGTGCCTGTTGTTTCAAAAACCAACATCAAGGAATACAAAGATAGCTT
  CTCTAATGAGAAATTTGATTTTCGCAACCATCCAAACATCACTTTCTTTGTTTATGTCAGTAATTTCACT
  TGGCCCATCAAAATTCAGATTGCCTTCTCCCAGCACAGCAACTTCATGGACCTGGTACAGTTCTTCGTGA
  CTTTCTTCAGTTGTTTCCTCTCGCTGCTTCTGGTGGCTGCAGTGGTCTGGAAGATCAAGCAGAGCTGTTG
  GGCATCCAGGCGGAGAGAGCAACTTCTTCGGGAGATGCAACAGATGGCCAGCCGCCCCTTTGCTTCTGTA
  AACGTTGCCTTGGAAACAGATGAAGAACCTCCTGATCTCATTGGGGGAAGTATAAAGACCGTTCCTAAGC
  CCATTGCCCTGGAGCCCTGCTTTGGTAACAAAGCCGCAGTCCTCTCTGTATTCGTGAGGCTCCCTCGAGG
  ACTGGGAGGAATCCCTCCTCCTGGTCAGTCAGGTCTCGCTGTGGCCAGTGCCCTGGTGGACATTTCTCAG
  CAGATGCCAATAGTGTACAAGGAGAAGTCAGGAGCTGTAAGAAACCGGAAGCAGCAGCCGCCTGCACAGC
  CTGGAACCTGCATT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218405 representing NM_009730
  Red=Cloning site Green=Tags(s)
  
  MVAVAAAAATEARLRGSTTATAAPAGRKGRQHRPCTATGAWRPGPRARLCLPRVLSRALPPPPLLPLLFS
  LLLLPLPREAEAAAVAAAVSGSAAAEAKECDRPCVNGGRCNPGTGQCVCPTGWVGEQCQHCGGRFRLTGS
  SGFVTDGPGNYKYKTKCTWLIEGQPNRIMRLRFNHFATECSWDHLYVYDGDSIYAPLIAAFSGLIVPERD
  GNETAPEVTVTSGYALLHFFSDAAYNLTGFNITYNFDMCPNNCSGRGECKSSNSSSAVECECSENWKGES
  CDIPHCTDNCGFPHRGICNASDTRGCSCFPHWQGPGCSIPVPANQSFWTREEYSDLKLPRASHKAVVNGN
  IMWVVGGYMFNHSDYSMVLAYDLTSREWLPLNHSVNSVVVRYGHSLALHKDKIYMYGGKIDSTGNVTNEL
  RVFHIHNESWVLLTPKAKDQYAVVGHSAHIVTLASGRVVMLVIFGHCPLYGYISVVQEYDLEKNTWSILH
  TQGALVQGGYGHSSVYDDRTKALYVHGGYKAFSANKYRLADDLYRYDVDTQMWTILKDSRFFRYLHTAVI
  VSGTMLVFGGNTHNDTSMSHGAKCFSSDFMAYDIACDRWSVLPRPELHHDVNRFGHSAVLYNSTMYVFGG
  FNSLLLSDVLVFTSEQCDAHRSEAACVAAGPGIRCLWDTQSSRCTSWELATEEQAEKLKSECFSKRTLDH
  DRCDQHTDCYSCTANTNDCHWCNDHCVPVNHSCTEGQISIAKYESCPKDNPMYYCNKKTSCRSCALDQNC
  QWEPRNQECIALPENICGNGWHLVGNSCLKITTAKENYDNAKLSCRNHNAFLASLTSQKKVEFVLKQLRL
  MQSSQSMSKLTLTPWVGLRKINVSYWCWEDMSPFTNSLLQWMPSEPSDAGFCGILSEPSTRGLKAATCIN
  PLNGSVCERPANHSAKQCRTPCALRTACGECTSSSSECMWCSNMKQCVDSNAYVASFPFGQCMEWYTMSS
  CPPENCSGYCTCSHCLEQPGCGWCTDPSNTGKGKCIEGSYKGPVKMPSQASAGNVYPQPLLNSSMCLEDS
  RYNWSFIHCPACQCNGHSKCINQSVCEKCEDLTTGKHCETCISGFYGDPTNGGKCQPCKCNGHASLCNTN
  TGKCFCTTKGVKGDECQLCEVENRYQGNPLKGTCYYTLLIDYQFTFSLSQEDDRYYTAINFVATPDEQNR
  DLDMFINASKNFNLNITWATSFPAGTQTGEEVPVVSKTNIKEYKDSFSNEKFDFRNHPNITFFVYVSNFT
  WPIKIQIAFSQHSNFMDLVQFFVTFFSCFLSLLLVAAVVWKIKQSCWASRRREQLLREMQQMASRPFASV
  NVALETDEEPPDLIGGSIKTVPKPIALEPCFGNKAAVLSVFVRLPRGLGGIPPPGQSGLAVASALVDISQ
  QMPIVYKEKSGAVRNRKQQPPAQPGTCI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009730
• ORF Size: 4284 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009730.2, NP_033860.2
• RefSeq Size: 8740 bp
• RefSeq ORF: 4287 bp
• Locus ID: 11990
• UniProt ID: Q9WU60
• Cytogenetics: 2 63.26 cM
• Gene Summary: This gene encodes a widely expressed transmembrane glycoprotein that plays important roles in diverse physiological processes such as regulation of hair pigmentation, monocyte-T cell interaction and control of energy homeostasis. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. Certain mutations in this gene are responsible for the mahogany mouse phenotype of dark brown or black coat on a normally agouti background. Mice with loss-of-function mutations in this gene exhibit black coat color, tremor, adiposity, higher basal metabolic rate, juvenile-onset hypomyelination and age-dependent spongiform neurodegeneration of the central nervous system. [provided by RefSeq, Jul 2016]