Chsy3 (NM_001081328) Mouse Tagged ORF Clone

CAT#: MG218256

Chsy3 (tGFP-tagged) - Mouse chondroitin sulfate synthase 3 (Chsy3), (10ug)

Product Images

Circular map for MG218256

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chsy3
• Synonyms: 4833446K15Rik; AI662215
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG218256 representing NM_001081328
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGTGCGCTCCCGCCGCCCATGGGTGAGCGTGGCATTGGGGTTGGTCCTGGGCTTCACCGCCGCGT
  CCTGGCTCATCGCCCCCCGGGTGGCCGAGCTGAGCGAGAAGAGGCGACGCGGCTCCAGTCTTTGCTCCTA
  CTACGGCCGCTCGGCTACCGGGCCCCGCGCGGACGCGCAGCAGCTGCTCCCCCAACCCCAGTCCCGGCCG
  CGGCTAGAGCAGTCGCCGCCCCCTGCCAGCCACGAGCTCCCCGGTCCTCAGCAGCCGGAGGCGGCGCCCG
  GAGGTCCCAGTTTTCGGAGCAGCCCCTGGCAGCAGCCGGCTCTGTTGCCGCAGAGGAGGCGAGGACACAC
  GCCCGAAGGTGCGACGGCGCTTCCCGGCGCTCCGGCTGCCAAAGGGGAACCAGAGGAGGAGGATGGGGGC
  GCGGCTGACCCTCGGAAGGGTGGCCGGCCGGGGAGCAGCCACAACGGCAGCGGGGACGGGGGTGCCGCTG
  TCCCGACCTCCGGACCCGGGGACTTCCTGTACGTGGGTGTGATGACCGCACAGAAGTACCTGGGCAGTCG
  CGCGCTGGCCGCGCAGCGGACCTGGGCGCGCTTCATCCCTGGCCGCGTGGAGTTCTTTTCCAGTCAGCAA
  TCTCCCAGTGCTGCGCTTGGCCAGCCCCCGCCACCTTTGCCTGTCATCGCGCTGCCAGGGGTCGACGATT
  CCTACCCTCCCCAGAAAAAGTCCTTCATGATGATCAAGTACATGCACGACCACTATCTGGACAAGTATGA
  GTGGTTCATGCGCGCCGACGACGATGTCTACATCAAAGGTGATAAGTTAGAAGAATTTCTAAGATCCCTA
  AATAGCAGCAAGCCTCTCTACCTGGGACAGACGGGCCTGGGCAATACTGAAGAACTTGGAAAGCTGGGGC
  TGGAGCCCGGGGAGAACTTCTGCATGGGAGGACCTGGCATGATCTTCAGCAGAGAGGTTCTCAGGCGGAT
  GGTGCCTCATATCGGCGAATGCCTCCGAGAGATGTACACCACACACGAAGACGTAGAAGTAGGAAGGTGT
  GTTCGCCGTTTCGGCGGGACGCAGTGTGTCTGGTCTTATGAGATGCAGCAGCTGTTCCATGAAAACTACG
  AACACAATCGCAAGGGTTACATCCAAGACCTCCACAACAGCAAAATCCACGCAGCCATCACGCTCCATCC
  GAACAAAAGGCCCGCGTACCAGTACAGACTTCATAACTACATGCTCAGCCGCAAGATCTCCGAGCTCCGC
  TACCGCACCATCCAGCTCCACCGGGAGAGCGCTCTCATGAGCAAGCTCAGCAACAGTGAAGTGAGCAAAG
  AGGACCAACAGCTGGGAAGGACGCCGTCCTTCAACCACTTCCAGCCTCGGGAGAGAAATGAAGTCATGGA
  GTGGGAGTTCCTGACGGGGAAGCTGCTTTACTCAGCTGCAGAGAACCAGCCTCCTCGACAGAGCATCAAC
  AGCATCCTAAGGTCAGCTCTGGATGACACTGTCCTGCAGGTGATGGAGATGATCAACGAGAATGCCAAGA
  GTAGGGGCCGACTCATCGACTTCAAGGAAATTCAGTATGGCTACCGCAGGGTTGATCCCATGCACGGGGT
  TGAGTACATATTGGACCTGCTACTCCTGTACAAAAGGCACAAAGGAAGGAAACTGACTGTGCCTGTGAGG
  CGCCATGCCTATCTTCAGCAGCCATTTAGCAAGCCTTTCTTCAGAGAGGTGGAAGAACTCGACGTCAACC
  GTCTGGTGGAGAGTATCAACAGCGGTACACAGTCATTCTCCGTTATATCCAATTCTCTAAAAATTCTCTC
  TTCTCTTCAAGAGGCCAAAGACATAGGAGGGCACAATGAAAAGAAAGTACACATTCTCGTTCCACTCGTT
  GGAAGGTACGACATTTTCTTGAGATTCATGGAAAATTTTGAAAGTACGTGTCTTATCCCAAAGCAAAATG
  TCAAGCTTGTCATCATCCTTTTCAGCAGGGATGCTGGCCAAGAGTCCATCAAGCACATTGAGCTGATACA
  AGAATATCAGAGCAGGTATCCCAGTGCAGAAATGATGCTCATTCCCATGAAGGGAGAGTTTTCCAGAGGT
  CTTGGTCTTGAAATGGCTTCTTCCCAGTTTGACAATGACACATTGCTGCTATTTTGTGATGTTGACTTGA
  TTTTCAGAGGAGACTTCCTCCAACGCTGTCGAGACAATACAGTTCAGGGACAACAGGTATATTACCCCAT
  CATCTTTAGCCAGTATGACCCAAAGGTCACCCATATGAGAAATCCTCCCACAGAGGGTGACTTTGTATTC
  TCAAAGGAAACTGGGTTTTGGAGAGACTATGGCTACGGAATCACATGCATTTACAAAAGCGATCTACTGG
  GTGCAGGTGGATTTGATACCTCAATACAAGGCTGGGGACTGGAAGATGTAGATCTCTATAATAAAGTCAT
  CCTATCTGGCTTACGGCCCTTCAGAAGTCAAGAAGTGGGAGTGGTGCATATTTTCCATCCTGTTCATTGT
  GATCCTAACTTGGACCCTAAGCAGTATAAGATGTGCTTAGGATCCAAAGCAAGTACTTTTGCCTCAACCA
  TGCAACTGGCTGAACTCTGGTTAGAAAAACATTTGGGTGTCAGGGATAATCGAACTCTCTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG218256 representing NM_001081328
  Red=Cloning site Green=Tags(s)
  
  MAVRSRRPWVSVALGLVLGFTAASWLIAPRVAELSEKRRRGSSLCSYYGRSATGPRADAQQLLPQPQSRP
  RLEQSPPPASHELPGPQQPEAAPGGPSFRSSPWQQPALLPQRRRGHTPEGATALPGAPAAKGEPEEEDGG
  AADPRKGGRPGSSHNGSGDGGAAVPTSGPGDFLYVGVMTAQKYLGSRALAAQRTWARFIPGRVEFFSSQQ
  SPSAALGQPPPPLPVIALPGVDDSYPPQKKSFMMIKYMHDHYLDKYEWFMRADDDVYIKGDKLEEFLRSL
  NSSKPLYLGQTGLGNTEELGKLGLEPGENFCMGGPGMIFSREVLRRMVPHIGECLREMYTTHEDVEVGRC
  VRRFGGTQCVWSYEMQQLFHENYEHNRKGYIQDLHNSKIHAAITLHPNKRPAYQYRLHNYMLSRKISELR
  YRTIQLHRESALMSKLSNSEVSKEDQQLGRTPSFNHFQPRERNEVMEWEFLTGKLLYSAAENQPPRQSIN
  SILRSALDDTVLQVMEMINENAKSRGRLIDFKEIQYGYRRVDPMHGVEYILDLLLLYKRHKGRKLTVPVR
  RHAYLQQPFSKPFFREVEELDVNRLVESINSGTQSFSVISNSLKILSSLQEAKDIGGHNEKKVHILVPLV
  GRYDIFLRFMENFESTCLIPKQNVKLVIILFSRDAGQESIKHIELIQEYQSRYPSAEMMLIPMKGEFSRG
  LGLEMASSQFDNDTLLLFCDVDLIFRGDFLQRCRDNTVQGQQVYYPIIFSQYDPKVTHMRNPPTEGDFVF
  SKETGFWRDYGYGITCIYKSDLLGAGGFDTSIQGWGLEDVDLYNKVILSGLRPFRSQEVGVVHIFHPVHC
  DPNLDPKQYKMCLGSKASTFASTMQLAELWLEKHLGVRDNRTLS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081328
• ORF Size: 2652 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081328.1, NP_001074797.1
• RefSeq Size: 3882 bp
• RefSeq ORF: 2655 bp
• Locus ID: 78923
• UniProt ID: Q5DTK1
• Cytogenetics: 18 D3
• Gene Summary: Has both beta-1,3-glucuronic acid and beta-1,4-N-acetylgalactosamine transferase activity. Transfers glucuronic acid (GlcUA) from UDP-GlcUA and N-acetylgalactosamine (GalNAc) from UDP-GalNAc to the non-reducing end of the elongating chondroitin polymer. Specific activity is much reduced compared to CHSY1.[UniProtKB/Swiss-Prot Function]