Dach1 (NM_001038610) Mouse Tagged ORF Clone
CAT#: MG217834
- TrueORF®
Dach1 (tGFP-tagged) - Mouse dachshund 1 (Drosophila) (Dach1) transcript variant 2, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001038610" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Dach1 |
Synonyms | Dac; Dach |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG217834 representing NM_001038610
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGTGCCGGCGGCTTTGATCCCTCCGACCCAGCTGGTCCCCCCTCAACCCCCGATCTCTACTTCTG CTTCCTCCTCGGGCACCACCACCTCCACCTCCTCGGCGACCTCGTCTCCGGCTCCATCCATCGGACCCCC GGCGTCGTCTGGGCCAACTCTGTTCCGGCCGGAGCCCATTGCCTCTTCTGCTTCTTCTTCAGCCGCGGCC ACAGTCACCTCTCCTGGTGGCGGCGGCGGCGGCAGCGGAGGCGGCGGTGGCAGCGGCGGCAACGGAGGCG GCGGCGGGAGCAACTGCAACCCCAGCCTGGCGGCCGGGAGCAGCGGCGGCGGCGTTAGCGCTGGCGGCGG CGGCGCCTCCAGCACCCCCATCACCGCGAGCACCGGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGCAGT AGCAGCAGCAGCAGCAGTAGCAGCAGCAGCAGCAGTAGCAGCAGCAGCTGCGGCCCCCTCCCTGGGAAAC CCGTGTACTCAACCCCGTCCCCAGTGGAAAACACCCCCCAGAATAATGAGTGCAAAATGGTGGATCTGAG AGGGGCCAAAGTGGCTTCCTTTACGGTGGAGGGCTGCGAGCTGATCTGCCTGCCCCAGGCTTTCGACCTG TTCCTGAAGCACTTGGTGGGGGGCTTGCACACCGTCTACACCAAGCTGAAGCGGTTGGAGATCACGCCGG TGGTGTGCAATGTGGAACAGGTTCGCATCCTGAGGGGACTGGGGGCCATCCAGCCCGGAGTGAACCGCTG CAAACTCATCTCCAGGAAGGACTTCGAGACCCTCTACAATGACTGCACCAACGCCAGTTCCAGACCTGGA AGGCCTCCTAAGAGGACTCAAAGTGTCACTTCCCCAGAGAACTCTCACATCATGCCGCATTCTGTCCCTG GCCTCATGTCTCCTGGAATCATTCCACCAACAGGTCTGACTGCAGCTGCTGCAGCTGCTGCAGCTGCTAC CAATGCAGCTATTGCTGAAGCAATGAAGGTGAAAAAAATAAAATTAGAAGCTATGAGCAACTATCATGCC AGTAACAACCAACATGGAGCAGATTCTGAAAACGGGGACATGAATTCAAGTGTTGGACTGGAACTTCCTT TTATGATGATGCCCCACCCTCTCATTCCTGTCAGCCTACCTCCAGCATCTGTCACCATGGCAATGAGTCA GATGAACCACCTTAGCACCATTGCAAATATGGCGGCGGCAGCACAAGTTCAGAGTCCTCCATCCAGGGTG GAGACATCTGTTATTAAGGAGCGTGTTCCCGACAGCCCCTCGCCTGCTCCATCTCTGGAGGAGGGCCGGA GGCCCGGCAGCCACCCATCCTCACACCGCAGCAGCAGTGTGTCCAGCTCCCCGGCGCGGACTGAGAGTTC TTCCGACAGAATCCCTGTCCATCAGAATGGCCTGTCCATGAACCAGATGCTTATGGGTTTATCCCCAAAT GTGCTTCCTGGGCCAAAGGAGGGGGATTTGGCTGGTCATGACATGGGGCATGAGTCAAAACGGATCCACA TTGAAAAAGATGAGACCCCACTTTCCACACCAACCGCAAGAGACAGCATCGACAAACTTTCTCTAACTGG GCATGGACAACCACTACCTCCCGGCTTTCCATCTCCCTTTCTGTTTCCTGATGGCCTGTCTTCCATAGAG ACCCTTCTCACTAACATACAGGGCCTCTTGAAAGTTGCCATAGACAATGCCAGAGCTCAAGAAAAGCAGG TCCAACTGGAAAAAACAGAGCTGAAGATGGATTTTTTAAGAGAAAGAGAACTAAGAGAAACACTGGAGAA GCAGCTGGCCATGGAACAAAAGAACAGAGCCATAGTTCAAAAGAGGCTAAAGAAGGAAAAGAAAGCAAAG AGAAAACTGCAGGAGGCACTAGAATTTGAGACAAAACGCCGTGAGCAAGCAGAGCAGACACTGAAACAGG CAGCTTCAGCGGACAGTCTCCGGGTCTTAAATGACTCCCTGACCCCTGAGATAGAAGCTGACCGCAGCGG AGGGAGAGCAGATGCTGAAAGGACAATACAAGATGGAAGACTGTATTTGAAAACTACTGTCATGTAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217834 representing NM_001038610
Red=Cloning site Green=Tags(s) MAVPAALIPPTQLVPPQPPISTSASSSGTTTSTSSATSSPAPSIGPPASSGPTLFRPEPIASSASSSAAA TVTSPGGGGGGSGGGGGSGGNGGGGGSNCNPSLAAGSSGGGVSAGGGGASSTPITASTGSSSSSSSSSSS SSSSSSSSSSSSSSSSCGPLPGKPVYSTPSPVENTPQNNECKMVDLRGAKVASFTVEGCELICLPQAFDL FLKHLVGGLHTVYTKLKRLEITPVVCNVEQVRILRGLGAIQPGVNRCKLISRKDFETLYNDCTNASSRPG RPPKRTQSVTSPENSHIMPHSVPGLMSPGIIPPTGLTAAAAAAAAATNAAIAEAMKVKKIKLEAMSNYHA SNNQHGADSENGDMNSSVGLELPFMMMPHPLIPVSLPPASVTMAMSQMNHLSTIANMAAAAQVQSPPSRV ETSVIKERVPDSPSPAPSLEEGRRPGSHPSSHRSSSVSSSPARTESSSDRIPVHQNGLSMNQMLMGLSPN VLPGPKEGDLAGHDMGHESKRIHIEKDETPLSTPTARDSIDKLSLTGHGQPLPPGFPSPFLFPDGLSSIE TLLTNIQGLLKVAIDNARAQEKQVQLEKTELKMDFLRERELRETLEKQLAMEQKNRAIVQKRLKKEKKAK RKLQEALEFETKRREQAEQTLKQAASADSLRVLNDSLTPEIEADRSGGRADAERTIQDGRLYLKTTVMY TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001038610 |
ORF Size | 2097 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001038610.1, NP_001033699.1 |
RefSeq Size | 5069 bp |
RefSeq ORF | 2100 bp |
Locus ID | 13134 |
UniProt ID | Q9QYB2 |
Cytogenetics | 14 48.25 cM |
Gene Summary | Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1 (By similarity). Binds to chromatin DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC220564 | Dach1 (untagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 2, (10ug) |
USD 705.00 |
|
MR217834 | Dach1 (Myc-DDK-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 2 |
USD 641.00 |
|
MR217834L3 | Lenti ORF clone of Dach1 (Myc-DDK-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 2 |
USD 941.00 |
|
MR217834L4 | Lenti ORF clone of Dach1 (mGFP-tagged) - Mouse dachshund 1 (Drosophila) (Dach1), transcript variant 2 |
USD 941.00 |
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