Lnx1 (NM_001159578) Mouse Tagged ORF Clone
CAT#: MG217650
- TrueORF®
Lnx1 (tGFP-tagged) - Mouse ligand of numb-protein X 1 (Lnx1) transcript variant 3, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001159578" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Lnx1 |
Synonyms | Lnx |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG217650 representing NM_001159578
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGCGCTGCTGCTTCTGGTCCTGCCCTGGCTGAGTCCAGCTAACTACATTGACAATGTGGGCAACC TGCACTTCCTGTACTCGGAACTCTGCAAAGGGGCCTCCCACTATGGCCTGACCAAGGACAGGAAAAGGCG CTCTCAAGATGGCTGTCCTGATGGTTGCGCCAGCCTCATGGCAACGACGCTCTCCCCAGAGGTTTCTGCA GCCGCCACCATCTCTTTAATGACAGATGAGCCTGGCCTAGACAACCCTGCCTACGTGTCCTCGGTGGAGG ATGGTGAGCCAGTAGCCAACTCCTCAGACTCTGGCCGGAGCAACCGGACTAGGGCACGGCCCTTTGAGCG CTCCACTATGAGAAGCCGGTCTTTCAAGAAGATCAACCGAGCTCTAAGTGCTCTCCGGAGGACGAAGAGC GGGAGTGTCGTTGCCAACCATGTTGACCAGGGCAGGGACAACTCTGAGAACACCACTGTCCCCGAAGTCT TCCCAAGGTTGTTTCACCTGATTCCAGATGGTGAAATTACAAGCATCAAAATCAACAGAGCGGATCCCAG CGAAAGCCTCTCCATAAGGCTGGTGGGGGGCAGCGAGACGCCGCTGGTCCACATCATCATCCAGCATATT TATCGCGATGGAGTGATTGCCAGAGATGGACGGCTGCTGCCAGGAGACATCATTCTCAAGGTCAACGGCA TGGACATCAGCAATGTCCCTCATAACTATGCCGTGCGGCTCCTGCGGCAGCCCTGCCAGGTGCTGCGGCT AACGGTGCTGCGGGAGCAGAAGTTCCGTAGCAGAAGCAATGCGCACGTGCCGGACTCCTATGGACCTCGG GATGACAGCTTCCATGTAATCCTCAACAAAAGCAGCCCCGAGGAGCAGCTTGGCATAAAGCTGGTGCGCC GGGTGGATGAGCCCGGCGTGTTCATCTTCAACGTGCTAAATGGGGGTGTCGCCGACCGACACGGCCAGCT GGAGGAGAATGACCGTGTGCTGGCCATCAACGGGCATGACCTTCGATTTGGCAGTCCAGAGAGTGCAGCT CATCTGATTCAGGCCAGTGAAAGACGCGTCCATCTCGTCGTGTCCCGCCAGGTTCGACAGTCCAGCCCAG ACATCTTTCAAGAAGCTGGCTGGATCAGCAATGGCCAGCAATCCCCAGGCCCAGGAGAGAGGAACACAGC TTCTAAGCCTGCAGCCACCTGTCATGAGAAGGTTGTAAGTGTCTGGAAAGACCCCAGCGAGTCTCTCGGC ATGACCGTCGGAGGGGGAGCATCCCACAGGGAATGGGACTTGCCCATCTATGTCATCAGTGTTGAACCCG GCGGAGTCATAAGCAGAGACGGGAGAATAAAAACAGGTGACATTTTATTGAATGTGAATGGGATTGAGCT CACAGAGGTCAGCCGGACAGAGGCCGTTGCCATACTGAAGAGCGCACCCTCCTCAGTGGTACTCAAAGCC TTGGAAGTCAAGGAACAGGAGGCCCAGGAAGACTGCAGCCCAGCAGCCCTGGACTCCAACCACAACGTGA CCCCGCCTGGTGACTGGTCCCCATCCTGGGTCATGTGGCTGGAATTACCACAGTACTTATGTAACTGTAA AGATGTGATACTGCGAAGGAACACAGCTGGAAGCCTGGGCTTCTGCATTGTGGGAGGTTATGAAGAATAC AGTGGGAACAAACCTTTTTTTATCAAGTCCATTGTTGAAGGAACACCTGCATACAATGACGGAAGAATCA GATGTGGTGACATTCTTCTCGCTGTCAACGGTAGAAGTACATCGGGTATGATACACGCTTGCCTGGCCAG GATGCTCAAGGAACTTAAAGGGAGAATTACTCTGACCATTGCTTCTTGGCCTGGTACTTTTTTA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217650 representing NM_001159578
Red=Cloning site Green=Tags(s) MKALLLLVLPWLSPANYIDNVGNLHFLYSELCKGASHYGLTKDRKRRSQDGCPDGCASLMATTLSPEVSA AATISLMTDEPGLDNPAYVSSVEDGEPVANSSDSGRSNRTRARPFERSTMRSRSFKKINRALSALRRTKS GSVVANHVDQGRDNSENTTVPEVFPRLFHLIPDGEITSIKINRADPSESLSIRLVGGSETPLVHIIIQHI YRDGVIARDGRLLPGDIILKVNGMDISNVPHNYAVRLLRQPCQVLRLTVLREQKFRSRSNAHVPDSYGPR DDSFHVILNKSSPEEQLGIKLVRRVDEPGVFIFNVLNGGVADRHGQLEENDRVLAINGHDLRFGSPESAA HLIQASERRVHLVVSRQVRQSSPDIFQEAGWISNGQQSPGPGERNTASKPAATCHEKVVSVWKDPSESLG MTVGGGASHREWDLPIYVISVEPGGVISRDGRIKTGDILLNVNGIELTEVSRTEAVAILKSAPSSVVLKA LEVKEQEAQEDCSPAALDSNHNVTPPGDWSPSWVMWLELPQYLCNCKDVILRRNTAGSLGFCIVGGYEEY SGNKPFFIKSIVEGTPAYNDGRIRCGDILLAVNGRSTSGMIHACLARMLKELKGRITLTIASWPGTFL SGPTRTRRLE - GFP Tag - V |
Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001159578 |
ORF Size | 1707 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001159578.1, NP_001153050.1 |
RefSeq Size | 3098 bp |
RefSeq ORF | 1710 bp |
Locus ID | 16924 |
UniProt ID | O70263 |
Cytogenetics | 5 C3.3 |
Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of NUMB. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates ubiquitination of isoform p66 and isoform p72 of NUMB, but not that of isoform p71 or isoform p65.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219054 | Lnx1 (untagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 3, (10ug) |
USD 584.00 |
|
MR217650 | Lnx1 (Myc-DDK-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 3 |
USD 643.00 |
|
MR217650L3 | Lenti ORF clone of Lnx1 (Myc-DDK-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 3 |
USD 943.00 |
|
MR217650L4 | Lenti ORF clone of Lnx1 (mGFP-tagged) - Mouse ligand of numb-protein X 1 (Lnx1), transcript variant 3 |
USD 943.00 |
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