Eif2b4 (NM_001127356) Mouse Tagged ORF Clone
CAT#: MG217613
- TrueORF®
Eif2b4 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2B subunit 4 delta (Eif2b4) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001127356" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Eif2b4 |
Synonyms | Eif2b |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG217613 representing NM_001127356
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAACCCAGCAGCCAGCTGCGCCGACGAGTCTCCCCAAGTCCTCCCGGAGCCTGTCTGGCTCACTCT GTGCCCTGTTTTCTGATGCAGAATCGAGATCCGAGATGAAGACTGAACTTTCTCCTCGGCCCGGGGCAGC AGGGCGGGAGTTGACCCAAGAAGAGAAGCTTCAGCTTCGAAAAGAAAAGAAACAGCAGAAGAAGAAACGG AAGGAGGAAAAGGGGGCAGACCAAGAAATTGGCTCTGCTGTGTCTGCAGCTCAACGGCAAGACCCAATCA GAGAACTCCCAGGACCTGGTAGTCAGCTGGGAGGTACTGCTGGGGAGAAACTTCCAGCTGGCCGGAGTAA GGCAGAACTTCGCGCCGAAAGGAGAGCCAAGCAGGAGGCAGAGCGGGCCCTGAAACAGGCAAGAAAAGGG GAACAAGGAGGCGTCCCTCCTCAGGCCTGCCCCAGCACTGCTGGAGAAACCACCTCAGGAGTGAAGCGTG TCCCCGAGCACACCCCAGCTGATGACCCCACACTTCTGAGGAGGCTCCTTAGAAAGCCAGATCGACAACA GGTTCCTACACGAAAGGATTACGGATCCAAAGTCAGTCTCTTCTCCCACCTGCCTCAGTACAGCAGACAA AGCTCCTTGACCCAGTACATGAGCATCCCATCCTCTGTGATCCACCCAGCCATGGTGAGACTCGGTCTGC AGTACTCCCAGGGCCTCATCAGTGGCTCCAATGCCCGGTGCATCGCACTGCTTCACGCCCTGCAGCAGGT GATTCAGGATTACACAACACCTCCCAGTGAGGAACTCTCCAGGGATCTTGTAAATAAACTAAAACCCTAC ATCAGCTTCCTGACGCAGTGCCGCCCCATGTCGGCAAGTATGTGCAACGCCATCAAGTTCCTAACCAAGG AAGTCACTGGCATGAGCAGCTCCAAGCGGGAAGAGGAGGCCAAGTCAGAACTTAGAGAAGCCCTCGACCG GTACGTGCAAGAGAAGATCGTGCTTGCAGCTCAGGCAATTTCACGATTTGCTTCTACAAAGATCAGTGAT GGGGACGTGATCCTGGTATATGGATGCTCATCTTTGGTGTCGAGAATTCTCCAGGAGGCCAGGGTCGAGG GCAGGCGGTTTCGGGTGGTGGTGGTGGACAGCCGGCCCCGGCTGGAAGGAAGGCACATGCTCCACTCTCT TGTCCGTGCTGGGGTCCCTACCTCCTATCTGCTGATTCCTGCAGCCTCCTATGTGCTTCCAGAGGTTTCT AAGGTGCTATTGGGAGCTCATGCACTCCTGGCCAATGGATCTGTGATGTCTCGGGTAGGGACAGCACAGT TGGCCCTGGTGGCTCGAGCTCATAACGTTCCAGTACTGGTCTGCTGTGAAACGTACAAGTTCTGTGAACG TGTGCAGACTGATGCCTTTGTCTCCAACGAGCTAGATGATCCCGATGATCTGCAGTGTAAGCGGGGAGAC CAGGTGGCCCTGGCTAACTGGCAGAGCCACCCGTCCCTCCGGTTGTTGAATCTGGTCTATGACGTGACTC CACCTGAGCTCGTGGATCTGGTGATCACAGAGTTGGGCATGATCCCTTGCAGTTCTGTGCCTGTTGTTCT TCGAGTCAAGAGCAGTGACCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217613 representing NM_001127356
Red=Cloning site Green=Tags(s) MPTQQPAAPTSLPKSSRSLSGSLCALFSDAESRSEMKTELSPRPGAAGRELTQEEKLQLRKEKKQQKKKR KEEKGADQEIGSAVSAAQRQDPIRELPGPGSQLGGTAGEKLPAGRSKAELRAERRAKQEAERALKQARKG EQGGVPPQACPSTAGETTSGVKRVPEHTPADDPTLLRRLLRKPDRQQVPTRKDYGSKVSLFSHLPQYSRQ SSLTQYMSIPSSVIHPAMVRLGLQYSQGLISGSNARCIALLHALQQVIQDYTTPPSEELSRDLVNKLKPY ISFLTQCRPMSASMCNAIKFLTKEVTGMSSSKREEEAKSELREALDRYVQEKIVLAAQAISRFASTKISD GDVILVYGCSSLVSRILQEARVEGRRFRVVVVDSRPRLEGRHMLHSLVRAGVPTSYLLIPAASYVLPEVS KVLLGAHALLANGSVMSRVGTAQLALVARAHNVPVLVCCETYKFCERVQTDAFVSNELDDPDDLQCKRGD QVALANWQSHPSLRLLNLVYDVTPPELVDLVITELGMIPCSSVPVVLRVKSSDQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001127356 |
ORF Size | 1632 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001127356.1, NP_001120828.1 |
RefSeq Size | 2119 bp |
RefSeq ORF | 1635 bp |
Locus ID | 13667 |
UniProt ID | Q61749 |
Cytogenetics | 5 B1 |
Gene Summary | Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218164 | Eif2b4 (untagged) - Mouse eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 1, (10ug) |
USD 558.00 |
|
MR217613 | Eif2b4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 1 |
USD 557.00 |
|
MR217613L3 | Lenti ORF clone of Eif2b4 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 1 |
USD 857.00 |
|
MR217613L4 | Lenti ORF clone of Eif2b4 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2B, subunit 4 delta (Eif2b4), transcript variant 1 |
USD 857.00 |
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