Rspo4 (NM_001040689) Mouse Tagged ORF Clone
CAT#: MG217176
- TrueORF®
Rspo4 (tGFP-tagged) - Mouse R-spondin family member 4 (Rspo4) transcript variant 1, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
AAV Particle: DDK
"NM_001040689" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Rspo4 |
Synonyms | A730099F22; A930029K19Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG217176 representing NM_001040689
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGGCTCCACTCTGCCTGCTGCTGCTCCTCGCCCACGCAGTGGACATGCTCGCCCTGTACCGAAGGA AGAAGCAAGCAGGCACTGGTCTGGGGGGCAACTGCACGGGCTGTGTCATATGCTCGGAGGAGAATGGCTG TTCCACCTGCCAGCAGAGGCTCTTCCTCTTCATCCGCCGTGAAGGCATCCGTCAGTACGGCAAGTGTGTG CACGACTGTCCCCTTGGCTTCTTTGGGATCCGTGGCCAGGAGGCCAACAGGTGCAAGAAATGTGGGGCCA CGTGCGAGAGCTGTTTCAGCCAGGACTTCTGTATCCGCTGCAAAAGGCGGTTTCACCTATACAAAGGGAA GTGCCTGCCCTCCTGTCCACCGGGCACCTTGACCCACCAGAGTACCCGGGAGTGCCAGGAAGAGTGTGAG CCGAGCCCTTGGGGCAGCTGGAGTCCCTGCATACACAATGGGAAGACCTGTGGCTCAGGCTGGGGCCTAG AAACCCGGGTCCGGGAGGCTGGCCCGGCCAAGCAGGAAGAGACGGCGAGTTGCCGAGTGTTATCTGAGTC CAGGAAGTGTCCTATAAAGAGACTCTGCCCAGGAGAAAGAAATCCCAGGCAGAAGAACAGGAAGGATCGG CGCCAGCGTAAGGACAGGAAGTTGGAGCGCAGGCCACACCAGCGTGGCTCACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217176 representing NM_001040689
Red=Cloning site Green=Tags(s) MRAPLCLLLLLAHAVDMLALYRRKKQAGTGLGGNCTGCVICSEENGCSTCQQRLFLFIRREGIRQYGKCV HDCPLGFFGIRGQEANRCKKCGATCESCFSQDFCIRCKRRFHLYKGKCLPSCPPGTLTHQSTRECQEECE PSPWGSWSPCIHNGKTCGSGWGLETRVREAGPAKQEETASCRVLSESRKCPIKRLCPGERNPRQKNRKDR RQRKDRKLERRPHQRGSQ TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_001040689 |
ORF Size | 684 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001040689.1, NP_001035779.1 |
RefSeq Size | 2371 bp |
RefSeq ORF | 687 bp |
Locus ID | 228770 |
UniProt ID | Q8BJ73 |
Cytogenetics | 2 G3 |
Gene Summary | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC212832 | Rspo4 (untagged) - Mouse R-spondin family, member 4 (Rspo4), transcript variant 1, (10ug) |
USD 330.00 |
|
MR217176 | Rspo4 (Myc-DDK-tagged) - Mouse R-spondin family, member 4 (Rspo4), transcript variant 1 |
USD 300.00 |
|
MR217176L3 | Lenti ORF clone of Rspo4 (Myc-DDK-tagged) - Mouse R-spondin family, member 4 (Rspo4), transcript variant 1 |
USD 600.00 |
|
MR217176L4 | Lenti ORF clone of Rspo4 (mGFP-tagged) - Mouse R-spondin family, member 4 (Rspo4), transcript variant 1 |
USD 600.00 |
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