Tmc3 (NM_177695) Mouse Tagged ORF Clone

CAT#: MG217139

Tmc3 (tGFP-tagged) - Mouse transmembrane channel-like gene family 3 (Tmc3), (10ug)

Product Images

Circular map for MG217139

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tmc3
• Synonyms: 9030203A06
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217139 representing NM_177695
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAAACCTCAAAGGCATCCCAGCGCTACAGAAGCATTCGGAGAAACGCCAGCCAGTGTTATCTCTACC
  AGGACTCCCTGCTTCTTGGTAACTCCGATGACAGCTTTAATGCTGATGAAACGGGAGACAGCAGTGATCC
  GGAACAAATCTTCCAGAATATACAGTTTCAGAAAGATCTCATGGCAAATATCCGCTGCAGACCCTGGACC
  ATGGGGCAGAAACTGCGGGCACTGAGACGAGCCAAGGAGATTGTGCTGAAGTTTGAAGGGAGGCTGACCA
  GGACTCGAGGCTACCAGGCAGCAGGAGCAGAGCTCTGGCGGAAATTCGCCCGTCTGGCCTGTAACTTTGT
  GGTCATCTTCATCCCCTGGGAAATGAGGATAAAGAAAATTGAGAGTCACTTCGGGTCTGGTGTGGCCTCC
  TACTTCATCTTCCTGAGGTGGCTATTTGGGATTAACATAGTGCTCACAGTGATGACCGGTGCCTTTGTTG
  TCCTTCCCGAGCTGATCGCAGGCCAGCCCTTTGGAAGTACAGCCAGCAAGACCATTCCCCGGGAGCAGAT
  CACATCTGCACAGGATCTGGACACTGTCTGGTCCCTGGGGGGCTACCTTCAATACTCCGTCCTGTTCTAC
  GGGTACTACGGAAGAGAGAGGCGGATCGGGAGAGCCGGCTACCGCCTGCCCTTGGCGTATTTCCTGGTGG
  GGATGGCAGTGTTTGCTTACAGCTTCATCGTTCTCTTAAAAAAGATGGCTAAGAACTCCCGCACCAGCCT
  AGCCAGTGCTTCCAATGAAAATTACACCTTCTGCTGGCGGGTGTTTTGTGCCTGGGATTATCTCATTGGG
  AACCCAGAGGCTGCAGAAAGCAAAACAGCTGCCATCTTGAACAGCATTAGGGAGGCCATACTGGAAGAGC
  AAGAAAAGAAAAAAAACAAAAACATGGCGGTGACTGTCTGTCTGAGAATCATTGCCAACATCCTGGTGCT
  CCTCTCACTGGCTGGAAGCATCTATCTCATCTACTTTGTGGTGGACCGGTCCCAGAAGCTGGAGCAGTCG
  AAGAAGGAGCTGACACTTTGGGAAAAGAATGAGGTAAGCGTGGTGGTCTCCCTCGTCACCATGCTAGCAC
  CGTCTGCCTTTGACCTCATCGCTGCCTTGGAGATGTATCATCCACGGACGACACTGCGCTTCCAGTTAGC
  AAGGGTCCTGGTACTGTATTTAGGAAATCTCTACAGCTTGATCATTGCTCTTCTGGACAAAGTGAACAGC
  ATGAACATTGAGGAAGCTGCTACTAAAAACATAACAAGCCACTGGGCAGATGCTCCCACCTTCTCTGCCA
  CCAGGACAGTGCCTGAAGAAGGGCAGTGGCCCACACCTGGATCAGGAGCGGAGCTCAGGAGAAACACTAG
  CACATGGGTCGTAGAAGAGACAAGCTTTCTGACTTCTATCACGCCACATACAAAGGCCAACAAGACAGTG
  CCCTACATGCAGGGCCCACAAGGCCAGTGCTGGGAGACATATGTCGGCCAGGAGATGCTGAAGCTGTCTG
  TCATCGACATGCTCTTCACTGTGGCCAGCATCCTCCTCATAGACTTCTTCCGAGGACTTTTTGTTCGCTA
  CCTAAGTGACTATTGGTGTTGGGACCTTGAAAGCAAGTTTCCTGAATATGGAGAATTCAAGATTGCCGAG
  AATGTGCTGCACCTTGTCTACAATCAAGGCATGATTTGGATGGGGGCCTTCTTCTCTCCGTGTCTGCCAG
  CATTCAATGTTCTTAAGCTCATTGGGCTGATGTACCTGAGGAGCTGGGCGGTGCTGACCTGCAATGTACC
  CCACCAGCAAGTGTTTCGAGCTTCCAGATCCAACAACTTCTACCTGGCAATGCTCCTGTTCATGTTGTTC
  CTTTGCATGCTGCCCACCATTTTTGCCATTGTCCACTACAAGCCGTCTCTGAACTGTGGTCCCTTCAGCG
  GACAAGAGAAAATTTATGACATCGTGTCCGAGACGATAGAGAATGACTTCCCGACCTGGTTTCACGCTGT
  AGTTGGACATATCAGCAGCCCCGTGGTGATACTTCCAGCTGTGCTGCTTCTGTTCATGCTCATCTACTAC
  CTTCAGAGCATCGCGAGATCACTCAAGCTCAGCAGCCAGCAACTCAGGATGCAGATCCAAAATGCGAGAT
  CTGAAGATAAGAAAAAGGTTGCCCAAATGGTAGAGGCCCTAGCCATCCCTAGCGATGCCAGGCAGGCAGG
  CTCAGCCACTGAAGCAGAGTCGAGTGAAAATTCAAAGCCAAAGACTCTCCAAGCTCGAATCCAGACTCAT
  GAGGAAAGCAGCAAGAGGCTTCTAAAGGACAGCGACCTTATCAGCCAGCTGTCCTCAGTGTATATGGCAA
  CATCCCCAAACAATGGCCATATGCTTAACTTCGACTCTCTGAGTAGCAAGAGCCTCAGGATGGAGGCAAT
  CACAAGGTCCCTGCCACAGAGTCCTGGACAAGGGTCCAGGGACCCCTGCTCTCCTCTTCTAGATGGATCT
  AGGTCAAGGCCAGAGCAGGACACCAACAGGCATCCACATAGGCCATGTTCCAGCACAAGCAACCTCCACA
  AGAACAGATCCTGCTCATCTGTGACACAGACACAGCCCCTCAAAGATGTCCGCTCAGAGCCTCTTTCCCG
  GAAAGACTTTCAGCCAATCAGTCCTCCTTTCTGTGGATCTGGGGTCTCTACCTTGATGACACATGACCAC
  AGTCCCCGTGCTCCCAGGTACTATGTGGTTAATGAACGTGACTCTCACAAAAAGACCCACCGAGCTTTCT
  GGCCAGAAAGGCATTTCAAGATAGATGCTTTAGGTGACATTGTAGAGCTGTACCCCAGGAATGTGCAGCA
  GTACATGTCCTGGGTTCCCAACCAGCCTTGCTCTCCACAGCTGAGTGAAGAAGAGGAGGAGATGCTCAGG
  AGAGATTTGGTCCAGTGGTCAATCCCTGCTAGCTCCCTGACAGACCTGCCTCGGTCTTCTTGTTTTTACA
  CTGGTGACAGATCAGAAAATAACACCAGAGACCCTAAATACCAGCGAAGGGTGTACTACAGATCTGGGGA
  TAATAGTTTTGAAGACCAGCTTGAGAGGCCCACATTTGTACACAGAAAGCCACGATCCAGGAACGGCCAA
  TACCCACAGCATGCCCTGAAGGCCAGAGTCAAGGCCAAGTTCGAGCCGTCCTTCACAGAATCTGATTCTG
  TGTCAGCAGCATCCAGCAGTGACCATCAAAACAGCAACAATGACCAATACCTGCATGTCATGTCCAGCCA
  GGGCAGATTCCCAAGGTCTGCCAGCCAGCTGGGCAGGAGGAAGGCCAAGTCCAGGCAGGTACTACCCACA
  GATCTAAATGATCTAATTTGTTCAAATGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217139 representing NM_177695
  Red=Cloning site Green=Tags(s)
  
  MKTSKASQRYRSIRRNASQCYLYQDSLLLGNSDDSFNADETGDSSDPEQIFQNIQFQKDLMANIRCRPWT
  MGQKLRALRRAKEIVLKFEGRLTRTRGYQAAGAELWRKFARLACNFVVIFIPWEMRIKKIESHFGSGVAS
  YFIFLRWLFGINIVLTVMTGAFVVLPELIAGQPFGSTASKTIPREQITSAQDLDTVWSLGGYLQYSVLFY
  GYYGRERRIGRAGYRLPLAYFLVGMAVFAYSFIVLLKKMAKNSRTSLASASNENYTFCWRVFCAWDYLIG
  NPEAAESKTAAILNSIREAILEEQEKKKNKNMAVTVCLRIIANILVLLSLAGSIYLIYFVVDRSQKLEQS
  KKELTLWEKNEVSVVVSLVTMLAPSAFDLIAALEMYHPRTTLRFQLARVLVLYLGNLYSLIIALLDKVNS
  MNIEEAATKNITSHWADAPTFSATRTVPEEGQWPTPGSGAELRRNTSTWVVEETSFLTSITPHTKANKTV
  PYMQGPQGQCWETYVGQEMLKLSVIDMLFTVASILLIDFFRGLFVRYLSDYWCWDLESKFPEYGEFKIAE
  NVLHLVYNQGMIWMGAFFSPCLPAFNVLKLIGLMYLRSWAVLTCNVPHQQVFRASRSNNFYLAMLLFMLF
  LCMLPTIFAIVHYKPSLNCGPFSGQEKIYDIVSETIENDFPTWFHAVVGHISSPVVILPAVLLLFMLIYY
  LQSIARSLKLSSQQLRMQIQNARSEDKKKVAQMVEALAIPSDARQAGSATEAESSENSKPKTLQARIQTH
  EESSKRLLKDSDLISQLSSVYMATSPNNGHMLNFDSLSSKSLRMEAITRSLPQSPGQGSRDPCSPLLDGS
  RSRPEQDTNRHPHRPCSSTSNLHKNRSCSSVTQTQPLKDVRSEPLSRKDFQPISPPFCGSGVSTLMTHDH
  SPRAPRYYVVNERDSHKKTHRAFWPERHFKIDALGDIVELYPRNVQQYMSWVPNQPCSPQLSEEEEEMLR
  RDLVQWSIPASSLTDLPRSSCFYTGDRSENNTRDPKYQRRVYYRSGDNSFEDQLERPTFVHRKPRSRNGQ
  YPQHALKARVKAKFEPSFTESDSVSAASSSDHQNSNNDQYLHVMSSQGRFPRSASQLGRRKAKSRQVLPT
  DLNDLICSNV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_177695
• ORF Size: 3390 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177695.4, NP_808363.3
• RefSeq Size: 4316 bp
• RefSeq ORF: 3393 bp
• Locus ID: 233424
• UniProt ID: Q7TQ69
• Cytogenetics: 7 D3
• Gene Summary: Probable ion channel.[UniProtKB/Swiss-Prot Function]