Srgap3 (NM_080448) Mouse Tagged ORF Clone

CAT#: MG217119

Srgap3 (tGFP-tagged) - Mouse SLIT-ROBO Rho GTPase activating protein 3 (Srgap3), (10ug)

Product Images

Circular map for MG217119

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Srgap3
• Synonyms: AI452337; Arhgap14; D130026O08Rik; Gbi; MEGAP; mKIAA0411; WRP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG217119 representing NM_080448
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCATCTCAAACTAAGTTCAAGAAAGACAAAGAGATCATTGCTGAATATGAAGCCCAAATAAAAGAGA
  TCCGCACGCAGCTGGTGGAGCAGTTCAAATGCCTGGAGCAGCAGTCCGAGTCGCGGCTGCAGCTGCTGCA
  AGACTTGCAGGAGTTCTTCCGCAGGAAAGCTGAGATTGAGCTGGAGTATTCCCGAAGCCTGGAGAAGCTG
  GCTGAGCGCTTCTCCTCCAAGATTCGCAGCTCCCGGGAGCACCAGTTCAAGAAGGATCAATACCTCCTCT
  CGCCCGTGAACTGTTGGTATCTGGTACTGCATCAGACTCGGCGGGAGAGCCGAGACCATGCCACCCTCAA
  CGACATCTTCATGAACAATGTCATCGTCCGCCTCTCCCAGATCAGTGAAGATGTCATCAGACTCTTCAAA
  AAGAGCAAAGAGATTGGCCTGCAGATGCATGAGGAGCTGCTGAAGGTCACCAACGAGCTCTACACAGTCA
  TGAAAACCTACCACATGTACCATGCAGAGAGCATCAGTGCAGAAAGCAAGCTGAAGGAGGCTGAGAAGCA
  GGAGGAGAAACAGTTTAACAAGTCAGGAGAGCTAAGCATGAACCTGCTCCGGCACGAGGACAGACCCCAG
  CGCCGCAGCTCCGTGAAGAAGATTGAGAAGATGAAGGAGAAGAGGCAGGCCAAGTATTCTGAGAACAAGC
  TCAAGTGCACGAAGGCCCGGAATGATTACCTGCTGAATCTGGCAGCCACCAACGCAGCTATCAGCAAATA
  CTACATCCACGATGTCTCTGATCTCATCGATTGCTGTGATTTGGGCTTTCACGCCAGCCTGGCCCGCACT
  TTTCGGACCTACCTCTCAGCAGAATATAACCTGGAGACCTCTCGCCATGAGGGTCTGGACGTCATAGAGA
  ATGCAGTGGACAACCTGGACTCCCGGAGTGACAAGCACACAGTCATGGATATGTGCAGCCAGGTCTTTTG
  CCCTCCACTCAAGTTTGAGTTCCAGCCCCACATGGGGGATGAGGTCTGCCAGGTCAGTGCACAGCAGCCT
  GTCCAGACAGAGCTGCTCATGCGGTACCACCAGCTGCAGTCCAGGCTGGCCACTCTCAAGATTGAGAATG
  AAGAGGTTCGGAAAACCCTGGATGCCACCATGCAGACCTTACAGGACATGCTGACCGTGGAAGACTTCGA
  TGTCTCTGACGCCTTCCAACACAGCCGATCTACAGAGTCCATAAAGTCAGCTGCTTCTGAGACCTACATG
  AGCAAAATCAACATCGCCAAAAGGAGAGCCAACCAGCAGGAAACAGAAATGTTTTATTTTACAAAATTTA
  AAGAATATGTGAATGGCAGTAACCTCATCACCAAGCTGCAAGCCAAGCATGACTTACTCAAGCAGACCCT
  GGGTGAAGGGGAGAGAGCAGAGTGCGGCACGACCAGGCCCCCCTGTCTTCCCCCTAAACCACAGAAAATG
  AGGAGACCTAGGCCTCTTTCAGTGTATAGCCATAAACTCTTTAACGGCAGTATGGAAGCGTTCATTAAGG
  ATTCAGGACAAGCTATACCACTTGTAGTTGAGAGCTGTATTCGATTCATCAATTTATATGGACTCCAGCA
  GCAGGGGATCTTCAGAGTTCCAGGGTCTCAGGTTGAAGTCAATGACATCAAGAATTCCTTTGAGAGAGGT
  GAAGACCCCCTCGTGGATGACCAAAATGAGCGAGATATCAATTCAGTTGCTGGTGTTTTAAAACTGTATT
  TCCGAGGACTGGAAAACCCACTCTTTCCTAAGGAAAGGTTTCAAGATTTGATATCTACTATTAAACTGGA
  GAACCCAGCTGACAGGGTGCACCCAATCCAGCAGATCCTCATCACCCTGCCCCGCGTGGTCATTGTGGTC
  ATGAGATACCTCTTTGCTTTCCTCAATCACCTTTCCCAGTACAGTGATGAGAACATGATGGATCCCTACA
  ACTTGGCCATCTGCTTCGGGCCCACCCTCATGCACATCCCCGATGGGCAGGACCCTGTATCCTGCCAGGC
  GCATGTCAATGAAGTCATCAAAACTATCATCATCCACCATGAAGCCATTTTCCCCAGCCCTCGGGAGCTA
  GAGGGACCCGTGTACGAAAAATGCATGGCTGGAGGGGAAGAGTACTGTGACAGCCCACACAGTGAGCCGG
  GGACCATTGATGAAGTTGACCATGACAATGGCACGGAGCCTCACACCAGTGACGAAGAAGTGGAGCAGAT
  TGAGGCCATCGCCAAGTTTGACTATGTGGGGCGGTCCCCTCGTGAACTATCCTTCAAAAAAGGGGCCTCA
  CTACTCCTGTATCACCGTGCCTCAGAGGACTGGTGGGAGGGCCGCCACAACGGTGTAGATGGACTCATCC
  CTCATCAGTACATAGTTGTACAGGACATGGACGATGCCTTCTCCGATAGCCTGAGCCAAAAGGCTGACAG
  CGAGGCCAGCAGTGGGCCACTGCTGGATGACAAGGCCTCTTCCAAGAATGACCTCCAGTCCCCCACAGAG
  CACATCTCTGATTACGGCTTTGGGGGGGTGATGGGCCGAGTGCGACTACGGTCCGACGGAGCAGCCATCC
  CCAGGCGCCGAAGTGGGGGCGACACACACAGCCCACCCCGGGGCTTGGGTCCCAGCATAGACACGCCACC
  CCGAGCTGCTGCCTGCCCCAGCAGCCCCCACAAAATCCCCCTCAGCCGGGGACGGATCGAGAGCCCTGAG
  AAGAGAAGGATGGCGACTTTCGGGAGCGCTGGCAGTATCAACTACCCTGACAAGAAGGCGCTGACAGAAG
  GGCTCTCCATGAGGTCGACTTGCGGTTCCACGCGACACAGCAGCCTAGGGGACCACAAGTCCCTGGAGGC
  TGAGGCCCTGGCAGAAGACATCGAGAAGACCATGAGCACCGCTCTGCATGAGTTGCGGGAACTTGAGAGA
  CAGAACACTGTCAAGCAGGCACCAGATGTGGTGCTGGACACCCTGGAGCCCCTGAAGAATCCACCAGGCC
  CCATCAGCTCAGAGCCCGCCAGCCCCCTGCACACCATTGTCATCCGAGACCCTGATGCTGCTATGCGCCG
  AAGCAGCAGCTCATCGACGGAGATGATGACCACCTTCAAGCCAGCCCTGTCAGCTCGCCTGGCTGGTGCC
  CAGCTGCGTCCGCCCCCCATGCGTCCGGTCAGACCTGTGGTTCAGCACCGATCCAGCAGCAGCAGCAGCT
  CAGGTGTGGGCAGCCCGGCAGTGACACCCACGGAGAAGATGTTCCCTAATAGCTCCTCGGACAAGTCTGG
  CACCATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG217119 representing NM_080448
  Red=Cloning site Green=Tags(s)
  
  MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQEFFRRKAEIELEYSRSLEKL
  AERFSSKIRSSREHQFKKDQYLLSPVNCWYLVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFK
  KSKEIGLQMHEELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGELSMNLLRHEDRPQ
  RRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAATNAAISKYYIHDVSDLIDCCDLGFHASLART
  FRTYLSAEYNLETSRHEGLDVIENAVDNLDSRSDKHTVMDMCSQVFCPPLKFEFQPHMGDEVCQVSAQQP
  VQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSDAFQHSRSTESIKSAASETYM
  SKINIAKRRANQQETEMFYFTKFKEYVNGSNLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPPKPQKM
  RRPRPLSVYSHKLFNGSMEAFIKDSGQAIPLVVESCIRFINLYGLQQQGIFRVPGSQVEVNDIKNSFERG
  EDPLVDDQNERDINSVAGVLKLYFRGLENPLFPKERFQDLISTIKLENPADRVHPIQQILITLPRVVIVV
  MRYLFAFLNHLSQYSDENMMDPYNLAICFGPTLMHIPDGQDPVSCQAHVNEVIKTIIIHHEAIFPSPREL
  EGPVYEKCMAGGEEYCDSPHSEPGTIDEVDHDNGTEPHTSDEEVEQIEAIAKFDYVGRSPRELSFKKGAS
  LLLYHRASEDWWEGRHNGVDGLIPHQYIVVQDMDDAFSDSLSQKADSEASSGPLLDDKASSKNDLQSPTE
  HISDYGFGGVMGRVRLRSDGAAIPRRRSGGDTHSPPRGLGPSIDTPPRAAACPSSPHKIPLSRGRIESPE
  KRRMATFGSAGSINYPDKKALTEGLSMRSTCGSTRHSSLGDHKSLEAEALAEDIEKTMSTALHELRELER
  QNTVKQAPDVVLDTLEPLKNPPGPISSEPASPLHTIVIRDPDAAMRRSSSSSTEMMTTFKPALSARLAGA
  QLRPPPMRPVRPVVQHRSSSSSSSGVGSPAVTPTEKMFPNSSSDKSGTM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_080448
• ORF Size: 3297 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080448.4, NP_536696.4
• RefSeq Size: 8887 bp
• RefSeq ORF: 3300 bp
• Locus ID: 259302
• UniProt ID: Q812A2
• Cytogenetics: 6 E3
• Gene Summary: GTPase-activating protein for RAC1 and perhaps CDC42, but not for RhoA small GTPase. May attenuate RAC1 signaling in neurons (By similarity).[UniProtKB/Swiss-Prot Function]