Pkp3 (NM_001162924) Mouse Tagged ORF Clone

CAT#: MG216860

Pkp3 (tGFP-tagged) - Mouse plakophilin 3 (Pkp3) transcript variant 2, (10ug)

Product Images

Circular map for MG216860

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pkp3
• Synonyms: 2310056L12Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216860 representing NM_001162924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCACCGCAGGATCTCGGACCCGGATGGAGCCCAGAAGGAATTGTCCTACAGCAGGGACCTCCA
  GAATGAGCCAGGGAGCCAGTGGGGGCCAGACCAGCGGCAAGCCTGAGACTGGTGTTTGCTCCCTGGCTCT
  GCCTTCGGACCTACAGCTGGATCGCCGGGGTGCGGAGGGACCGGAGGCTGATCGACTTAGAGCTGCCAGA
  GTCCAGGAGCAAGTCCGAGCCCGCCTCCTGCAGCTGGGCCAGCAGTCGCGGCACAATGGGTCTGCTGAGC
  TTGATGGCTCCGCGGAGAGCGCCAGAGGCATGCCCAGAGGTCAATATCACACTATGCAGACTGGCTTCAG
  CTCCCGATCCCAGGGCATGAGTGGAGACAAGACCTCGACCTTCCGGCCCATCGCCAAGCCGGCCTACAGC
  CCAGCTTCCTGGTCCTCCCGCTCAGCCGTGGATCTGACGTGCAGTCGGAGGCTGAGTTCTGCCCACAATG
  GGGGCAGTGCCTTTGGGGCTGTGGGATATGGGGGTACCCAGCCCACTCCACCTATGCCCACCCGGCCAGT
  GTCCTTCCATGAGCGAGGTGGGGCAGCCAGCAGAGCTGACTATGACACATTATCCCTGCGCTCACTGAGG
  CTGGGGCCAGGGGGCCTGGATGACCGTTACAGCGTGGTATCTGAGCAACTGGAGCCTGCAGCCGCCTCTA
  CCTACAGGGCCTATGCTTACGAGCGCCAGGCTAGCTCCGGCTCTAGCCGAGCAGGGGGCCTGGACTGGCC
  AGAGGCCACAGAGGGACCACCCAGCAGGACCATCCGTGCACCTGCCATGCGAACCCTGCAAAGATTTCAG
  AGCAGCCACCGAAGCCGTGGGGGTACCGGGTCAGTGTCAGGTGCCGGCTTGGAGCCAGTGGCCCGAGCCC
  CATCTGTGCGCAGCCTCAGTCTTAGCCTGGCTGACTCAGGCCACCTGCCAGATGTACGCGGGCTGGACAG
  CTACACGGGCCATCGAACCCTGCAGAGGCTCAGCAGTGGCTTTGATGACATTGACCTGCCTTCAGCAGTC
  AAGTACCTCATGGCCTCAGACCCCAACCTGCAGGTGCTGGGAGCAGCTTACATCCAGCACAGGTGTTACA
  GTGATGCCGCCGCCAAGAAGCAGGCTCGCAGCCTTCAGGCAGTGCCTAGACTGGTGAAGCTCTTCAACCA
  CGCCAACCAGGAGGTGCAGCGCCACGCCACAGGTGCCATGCGCAACCTCATCTACGACAATGTAGACAAC
  AAGCTGGCGTTGGTAGAGGAGAATGGCATTTTTGAGCTGTTGCGGACACTTCGAGAGCAGGATGATGAGC
  TACGCAAAAATGTTACAGGGATCCTATGGAACCTGTCCTCCAGTGACCACCTAAAGGACCGCCTCGCCCG
  GGATACACTGGAGCAGCTCACAGATCTGGTGCTGAGCCCCCTGTCAGGGGCAGGTGGGCCTCCCCTCATC
  CAGCAGAATGCCTCAGAGGCAGAGATCTTCTACAATGCTACTGGCTTCCTCAGGAACCTCAGCTCAGCCT
  CCCAGGCCACTCGGCAGAAGATGCGCGAGTGCCATGGGCTAGTGGATGCTCTGGTCACCTACATCAACCA
  TGCCCTGGACGTTGGCAAGTGTGAGGACAAGAGTGTGGAAAATGCTGTGTGTGTGCTGAGGAACCTGTCC
  TACCGCCTCTACGACGAGATGCCGCCATCAGCCTTGCAGCGGCTTGAAGGCCGGGGCCGCAGGGACATGG
  CAGGGGCTCCGCCTGGTGAAATGGTAGGCTGCTTCACACCTCAGAGTCGGCGGCTGCGAGAGCTTCCTCT
  CACAGCTGATGCACTCACCTTTGCCGAAGTATCTAAGGATCCCAAGGGCCTCGAATGGCTGTGGAGCCCC
  CAGATTGTGGGGTTGTACAACCGACTGCTGCAGCGCTGTGAACTAAATCGGCATACAACGGAGGCGGCCG
  CAGGGGCGCTACAGAATATCACCGCGGGCGACCGCAGGTGGGCAGGTGTGCTGAGCCGCCTGGCCCTGGA
  ACAGGAGCGAATCCTGAACCCCTTGCTGGACCGCGTTCGCACCGCTGACCACAACCAACTGCGCTCACTG
  ACTGGCCTCATCCGAAACCTGTCTCGGAATGCCAGGAACAAGGACGAGATGTCCACAAAGGTAGTGAGCC
  ACCTGATTGAGAAGCTTCCTGGTAGTGTGGGTGAGAAGTGTCCCCCAGCTGAGGTGCTGGTCAACATCAT
  AGCCGTGCTCAACAACCTGGTGGTAGCCAGCCCCATTGCTGCCCGGGACCTGCTCTACTTTGATGGCCTC
  CGAAAGCTGGTCTTCATCAAAAAGAAGAGGGATAGCCCCGACAGTGAGAAGTCTTCCCGGGCAGCCTCCA
  GCCTCTTGGCCAACCTCTGGCAGTACAGCAAGCTCCACCGTGACTTCCGGGCGAAAGGCTACCGGAAAGA
  GGACTTCCTGGGTCCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216860 representing NM_001162924
  Red=Cloning site Green=Tags(s)
  
  MEPTAGSRTRMEPRRNCPTAGTSRMSQGASGGQTSGKPETGVCSLALPSDLQLDRRGAEGPEADRLRAAR
  VQEQVRARLLQLGQQSRHNGSAELDGSAESARGMPRGQYHTMQTGFSSRSQGMSGDKTSTFRPIAKPAYS
  PASWSSRSAVDLTCSRRLSSAHNGGSAFGAVGYGGTQPTPPMPTRPVSFHERGGAASRADYDTLSLRSLR
  LGPGGLDDRYSVVSEQLEPAAASTYRAYAYERQASSGSSRAGGLDWPEATEGPPSRTIRAPAMRTLQRFQ
  SSHRSRGGTGSVSGAGLEPVARAPSVRSLSLSLADSGHLPDVRGLDSYTGHRTLQRLSSGFDDIDLPSAV
  KYLMASDPNLQVLGAAYIQHRCYSDAAAKKQARSLQAVPRLVKLFNHANQEVQRHATGAMRNLIYDNVDN
  KLALVEENGIFELLRTLREQDDELRKNVTGILWNLSSSDHLKDRLARDTLEQLTDLVLSPLSGAGGPPLI
  QQNASEAEIFYNATGFLRNLSSASQATRQKMRECHGLVDALVTYINHALDVGKCEDKSVENAVCVLRNLS
  YRLYDEMPPSALQRLEGRGRRDMAGAPPGEMVGCFTPQSRRLRELPLTADALTFAEVSKDPKGLEWLWSP
  QIVGLYNRLLQRCELNRHTTEAAAGALQNITAGDRRWAGVLSRLALEQERILNPLLDRVRTADHNQLRSL
  TGLIRNLSRNARNKDEMSTKVVSHLIEKLPGSVGEKCPPAEVLVNIIAVLNNLVVASPIAARDLLYFDGL
  RKLVFIKKKRDSPDSEKSSRAASSLLANLWQYSKLHRDFRAKGYRKEDFLGP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001162924
• ORF Size: 2466 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001162924.1, NP_001156396.1
• RefSeq Size: 2926 bp
• RefSeq ORF: 2469 bp
• Locus ID: 56460
• UniProt ID: Q9QY23
• Cytogenetics: 7 F5
• Gene Summary: May play a role in junctional plaques.[UniProtKB/Swiss-Prot Function]