Pak6 (NM_001145854) Mouse Tagged ORF Clone

CAT#: MG216829

Pak6 (tGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 6 (Pak6) transcript variant 2, (10ug)

Product Images

Circular map for MG216829

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pak6
• Synonyms: 4732456M09
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216829 representing NM_001145854
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCCGCAAGAAAAAGAAGAAACGTCCTGAGATATCAGCCCCGCAGAACTTCCAGCATCGTGTCCACA
  CCTCCTTTGACCCCAAGGAAGGCAAGTTTGTGGGTCTGCCCCCACAATGGCAGAACATTCTGGATACTCT
  GAGGCGGCCCAAGCCTGTGGTGGATCCTTCTCGAATCACACGGGTGCAGTTGCAGCCCATGAAGACAGTC
  GTACGGGGCAGCTCAGTGCCCACGGAGGGCTACATCTCGGGGCTGCTCAACGACATTCAGAAGTTGTCAG
  TTATCAGCTCCAACACCCTGCGTGGACGCAGCCCCACCAGCCGGCGGCGGGCACAGTCCCTGGGGCTGCT
  GGGAGATGATCAGTGGGCTGCTGACCCCGATATGTACCTCCAAAGCCCCCAGTCGGAGCACACTGATCCC
  CATGGCCTCTACCTCAGCTGCAATGGGGGCACACCAGCAGGTCACAGGCAGGTGCCCTGGCCAGAACCAC
  AGAGCCCGCAGGCCCTGCCCAATGGGATGGCTGCCAAGGCGCAGTCACTGGGCCCTGCTGAGTTCCAGGG
  TGCCTCACAACGCTGCCTGCAGCAGCTGGGTGCCTGCCTGCAGAGTTCCCCACCCGGTACCTCACTCCCT
  ATGGCCACAGGGAGGCGTGGGGTGAAGGTTGCCAAGCATAGCTCCGAAGAGGCCCGGCCACAGTCCTGCC
  TGGTGGGCTCGGCCATTGGCAGGCCAGGTGGAGAAGGCAGCCCTAGCCCTAAGAACCAGGAAAGCAGCCT
  CAAGCACCGGCTCTTCCGAAGCATGTTCCTGTCCACTCCTGCCACAGGCGCGGCAAGCAGCAGCAAGCCG
  GTCCCTCTGCCGCAGAACAAGCCCAATTCTGCTTTCCGACCACCACAAAAAGACTCCTCTTCAAACCTGG
  TAGCCAAGGCCCAGTCCTTGCCCTCAGAGCAGCCCATGGGGACCTTCAGTCCTCTGACCACCTCAGATAC
  CAGCAGCCCCCAGAAGTCCCTCCGCACAGTTCCAGCCGCTGGTCCACTTCCAGGCCGGTCTTCTCCAGCA
  GGCTCCCCCCGTACCCGGCATGCTCAGATCAGCACCAGCAATCTCTACCTGCCCCAGGACCCCACAGTGG
  CCAAGGGGGCCCTGGGTGGCGAAGACACGGGCATTGTGACCCATGAACAGTTCAAGGCTGCGCTCAGGAT
  GGTGGTGGACCAGGGTGACCCTCGGCTGCTGCTGGACAGCTACGTGAAGATTGGGGAGGGCTCCACGGGC
  ATTGTGTGTCTGGCCCGGGAGAAGCACTCAGGTCGCCAGGTAGCAGTCAAGATGATGGACCTCAGGAAGC
  AGCAACGCAGGGAGCTGCTCTTTAATGAGGTGGTGATCATGCGTGACTACCAGCACCTCAACGTGGTGGA
  GATGTACAAGAGTTACCTGGTAGGAGAGGAGCTGTGGGTGTTGATGGAGTTCCTGCAGGGCGGGGCCCTC
  ACAGACATCATCTCCCAAGTCAGACTGAATGAGGAGCAGATTGCCACTGTGTGTGAAGCTGTGCTTCAGG
  CCTTGGCTTACCTGCACGCCCAGGGTGTCATCCACCGAGATATCAAGAGTGATTCCATCCTGCTGACCCT
  TGATGGCAGGGTGAAGCTCTCAGACTTTGGGTTCTGTGCTCAGATCAGCAAAGATGTTCCCAAGAGAAAG
  TCCCTGGTAGGAACTCCATACTGGATGGCTCCTGAAGTGATCTCCAGATCCCTGTACGCTACTGAGGTGG
  ATATCTGGTCTCTGGGCATCATGGTGATTGAGATGGTGGATGGAGAACCCCCCTACTTCAGTGACTCCCC
  AGTGCAAGCCATGAAGAGGCTGCGGGACAGCGCCCCACCCAAGCTAAAGAACTCTTACAAGGTCTCTCCA
  GTGCTGCGAGACTTCCTGGATCGGATGCTGGTACGGGAACCCCAAGAGCGAGCCACGGCCCAGGAGCTCC
  TGGATCACCCGTTTCTGCTGCAGACGGGGCTGCCTGAGTGTCTGGTGCCTCTGATACAGCTCTACCGCAA
  GCAGACCTCCACCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216829 representing NM_001145854
  Red=Cloning site Green=Tags(s)
  
  MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTV
  VRGSSVPTEGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDDQWAADPDMYLQSPQSEHTDP
  HGLYLSCNGGTPAGHRQVPWPEPQSPQALPNGMAAKAQSLGPAEFQGASQRCLQQLGACLQSSPPGTSLP
  MATGRRGVKVAKHSSEEARPQSCLVGSAIGRPGGEGSPSPKNQESSLKHRLFRSMFLSTPATGAASSSKP
  VPLPQNKPNSAFRPPQKDSSSNLVAKAQSLPSEQPMGTFSPLTTSDTSSPQKSLRTVPAAGPLPGRSSPA
  GSPRTRHAQISTSNLYLPQDPTVAKGALGGEDTGIVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTG
  IVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHLNVVEMYKSYLVGEELWVLMEFLQGGAL
  TDIISQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRK
  SLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSAPPKLKNSYKVSP
  VLRDFLDRMLVREPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001145854
• ORF Size: 2046 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145854.1, NP_001139326.1
• RefSeq Size: 3662 bp
• RefSeq ORF: 2049 bp
• Locus ID: 214230
• UniProt ID: Q3ULB5
• Cytogenetics: 2 E5
• Gene Summary: Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD (By similarity).[UniProtKB/Swiss-Prot Function]