Ppp1r13b (NM_011625) Mouse Tagged ORF Clone

CAT#: MG216718

Ppp1r13b (tGFP-tagged) - Mouse protein phosphatase 1 regulatory (inhibitor) subunit 13B (Ppp1r13b), (10ug)

Product Images

Circular map for MG216718

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ppp1r13b
• Synonyms: AI449786; ASPP1; AW545810; p85; Tp53bp2; Trp53bp2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216718 representing NM_011625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCCGATGATATTAACCGTGTTCTTGAGCAACAATGAACAGATTTTAACAGAAGTTCCTATAACAC
  CAGAAACCACCTGTCGAGATGTCGTAGAATTCTGCAAGGAACCTGGAGAGGGCGGTTGCCACCTGGCTGA
  AGTGTGGAGAGGAAGTGAGCGTCCCATCCCCTATGACCACATGATGTATGAGCACCTGCAGAAGTGGGGG
  CCTCGCAGAGAAGAAGTAAAATTTTTTCTTCGTCATGAGGATTCTCCAACTGAGAGCAGTGAACAAGGTG
  CCCGTCAGACCCAAGAGCAGAGAACTCAGAGGAGTGTAGTAAATGTACCTGGAGAAAAGCGCACTGAAAA
  TGGGGTTGGGAATCCACGTGTTGAACTTACCCTCTCAGAACTTCAAGATATGGCAGCCAGGCAGCAGCAG
  CAAATTGAAAACCAGCAGCAAATGTTGGTTGCCAAGGAACAGCGTTTACATTTTCTAAAGCAACAGGAGC
  GCCGCCAGCAGCAGTCCGTTTCTGAAAATGAAAAGCTTCAGAAACTGAAAGAGCGAGTTGAAGCCCAGGA
  AAACAAGCTGAAGAAGATCCGGGCCATGAGAGGACAAGTTGACTACAGCAAGATCATGAATGGCAATCTG
  TCTGCTGAGATAGAAAGGTTCAGTGCCATGTTCCAGGAGAAGAAACAGGAAGTTCAGACTGCAATCTTAC
  GAGTTGATCAGCTTAGTCAGCAACTAGAAGATTTAAAGAAAGGAAAACTGAATGGGTTCCAGTCATACAA
  TGGCAGGTTGACAGGGCCAGCAGCAGTGGAGTTGAAACGACTCTACCAAGAGCTGCAGATTCGTAACCAG
  CTTAATCAGGAGCAGAATTCCAAGCTCCAGCAGCAGAAGGAGCTCTTGAACAAGCGCAACATGGAAGTGG
  CCATGATGGATAAGCGGATCAGTGAGCTGCGGGAGCGCCTGTATGGCAAGAAGATCCAGCTGAACCGTGT
  GAATGGCACATCGTCACCACAGTCACCCCTGAGCACATCTGGCCGGGTCGCTGCTGTGGGTCCTTATATC
  CAGGTTCCCAGTACTGGGGGGTTTCCTCTCCCAGGGGACCCTGTGAAGCCCCAGTCTCTCACCATCGCCT
  CAAGTGCTGCCCATGGAAGATCCAAATCTGCTAATGATGGAAACTGGCCACCATTAAAGCAGAATTCTGC
  TTCAGTGAAGTCAACTCAGATGACTGGAGACTGGAAGGACTCAGGCATGGAGGGGACCCTAAAGCAAGGT
  GCCATCTCAAGCCAGCCTTTGCCTTTGTCAGCACTGGGAGCTACAGAAAAGCTGGGCATCGAGATTGGCA
  AAGGGCCACCTCCCATACCTGGTGTGGGCAAGCCACTGCCCCCAAGTTATGGGACATATCCAAGTTCTGG
  GCCCTTGGGCCCGGGATCAACAAGCTCTCTGGAGAGGAGGAAAGAAGGAAGCTTGCCCAGACCTGGTGCA
  GGCCCACCGAGTCGGCAGAAACCTGCCCCGCTGCCCCCAGCAAGCAACGCTCCCCAGCCTGGCTCCTCAC
  AGCAGATTCAGCAGAGGATCTCAGTGCCACCAAGTCCCACATACCCGCCAGCGGGGCCACCTGCCTTTCC
  AACTGGAGATGGAAAACCTGAGCTTCCACTCACAGTGGCCATTAGGCCTTTTCTGGCTGATAAAGGGTCA
  AGGCCACAATCTCCCAGGAAAGGACCTCAGACAGTAAATTCAAGTTCTATATACTCCATGTACCTCCAGC
  AAGCCACACCACCTAAGAACTACCAGCCACCAGCGCATGGCACCTTAAATAAGTCAGTTAAAGCAGTGTA
  CGGTAAGCCTGTCCTGCCGTCAGGGTCAGCATCGCCATCACCCTTACCGTTTCTTCATGGGTCACTGGGC
  ACAGGCACGGCACAGCCTCAGCCACCTTCAGACTCTGCTGAGAAAGAGCCAGAGCAGGAAGGCCCCAGTG
  TACCTGGAGAAGGCAGCACAGTGGAAAGCCTGCCACGGCCGCTCAGTCCCACCAAACTCACACCCATCGT
  ACACTCCCCACTCCGTTATCAGAGTGATGCAGACCTGGAAGCTCTCCGCAGGAAGCTGGCCAATGCACCC
  CGACCCCTGAAGAAGCGCAGCTCTATCACAGAGCCAGAGGGCCCTGGAGGGCCCAACATTCAAAAGTTGC
  TTTACCAACGTTTCAACACCTTGGCTGGTGGAATGGAGGGTACCCCTTTCTACCAGCCCAGCCCTTCCCA
  GGACTTTGTGGGCACCTTAGCTGACATGGACAACGGAAACACCAATGCCAATGGCAACTTGGACGAGCCT
  TTCCCTCCCCGGCCCACGGCCCCACTTCCTGAGGAGCTTGCCCCTTCCTCAGATGCCAATGATAATGAGT
  TACCTTCCCCTGAGCCAGAGGAGCTCATTTGTCCCCAAACAACTCATCAAACTGCTGAGCCCACTGAAGA
  CAATAACAACAATGTGGCCCCAGTACCCTCCACAGAACAGATCCCAAGTCCTGTGGCTGAGGCTCCTTCA
  GAGGAGGATCAAGTCCCTCCAGCACCTCTTTCCCCCGTCATCCATCCTCCAGCAGCATCCGCGAGCAAAC
  GCACCAACCTGAAGAAGCCTAACTCTGAACGGACAGGGCACGGGCTGAGAGTGCGCTTCAACCCCCTAGC
  CCTGCTTCTGGACGCTTCTCTCGAAGGAGAGTTTGATCTAGTGCAGAGGATCATCTATGAGGTGGAGGAC
  CCTAGCAAACCCAATGACGAAGGGATCACCCCCCTACACAATGCTGTCTGTGCTGGCCACCACCATATCG
  TGAAGTTTCTGCTGGACTTTGGTGTCAATGTGAATGCTGCAGACAGTGACGGATGGACACCACTGCATTG
  TGCTGCCTCTTGCAACAGTGTCCACCTCTGCAAGCAGCTGGTGGAAAGTGGAGCCGCTATCTTTGCCTCC
  ACCATCAGTGACATTGAGACTGCTGCAGACAAGTGTGAAGAGATGGAAGAGGGATACATCCAGTGTTCCC
  AGTTTCTGTATGGGGTACAAGAGAAGCTGGGAGTGATGAACAAAGGCACCGTGTATGCTTTGTGGGACTA
  CGAGGCCCAGAACAGCGATGAGCTGTCCTTCCATGAAGGGGATGCCATCACCATCCTGAGGCGCAAAGAT
  GAAAACGAGACCGAGTGGTGGTGGGCTCGTCTTGGGGACCGGGAGGGCTACGTGCCCAAAAACTTGCTGG
  GGTTGTATCCACGGATCAAACCCCGGCAGCGAACACTTGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216718 representing NM_011625
  Red=Cloning site Green=Tags(s)
  
  MMPMILTVFLSNNEQILTEVPITPETTCRDVVEFCKEPGEGGCHLAEVWRGSERPIPYDHMMYEHLQKWG
  PRREEVKFFLRHEDSPTESSEQGARQTQEQRTQRSVVNVPGEKRTENGVGNPRVELTLSELQDMAARQQQ
  QIENQQQMLVAKEQRLHFLKQQERRQQQSVSENEKLQKLKERVEAQENKLKKIRAMRGQVDYSKIMNGNL
  SAEIERFSAMFQEKKQEVQTAILRVDQLSQQLEDLKKGKLNGFQSYNGRLTGPAAVELKRLYQELQIRNQ
  LNQEQNSKLQQQKELLNKRNMEVAMMDKRISELRERLYGKKIQLNRVNGTSSPQSPLSTSGRVAAVGPYI
  QVPSTGGFPLPGDPVKPQSLTIASSAAHGRSKSANDGNWPPLKQNSASVKSTQMTGDWKDSGMEGTLKQG
  AISSQPLPLSALGATEKLGIEIGKGPPPIPGVGKPLPPSYGTYPSSGPLGPGSTSSLERRKEGSLPRPGA
  GPPSRQKPAPLPPASNAPQPGSSQQIQQRISVPPSPTYPPAGPPAFPTGDGKPELPLTVAIRPFLADKGS
  RPQSPRKGPQTVNSSSIYSMYLQQATPPKNYQPPAHGTLNKSVKAVYGKPVLPSGSASPSPLPFLHGSLG
  TGTAQPQPPSDSAEKEPEQEGPSVPGEGSTVESLPRPLSPTKLTPIVHSPLRYQSDADLEALRRKLANAP
  RPLKKRSSITEPEGPGGPNIQKLLYQRFNTLAGGMEGTPFYQPSPSQDFVGTLADMDNGNTNANGNLDEP
  FPPRPTAPLPEELAPSSDANDNELPSPEPEELICPQTTHQTAEPTEDNNNNVAPVPSTEQIPSPVAEAPS
  EEDQVPPAPLSPVIHPPAASASKRTNLKKPNSERTGHGLRVRFNPLALLLDASLEGEFDLVQRIIYEVED
  PSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPLHCAASCNSVHLCKQLVESGAAIFAS
  TISDIETAADKCEEMEEGYIQCSQFLYGVQEKLGVMNKGTVYALWDYEAQNSDELSFHEGDAITILRRKD
  ENETEWWWARLGDREGYVPKNLLGLYPRIKPRQRTLA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011625
• ORF Size: 3261 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011625.1, NP_035755.1
• RefSeq Size: 4355 bp
• RefSeq ORF: 3264 bp
• Locus ID: 21981
• UniProt ID: Q62415
• Cytogenetics: 12 F1
• Gene Summary: Regulator that plays a central role in regulation of apoptosis via its interaction with p53/TP53. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo (By similarity).[UniProtKB/Swiss-Prot Function]