Trim36 (NM_001170855) Mouse Tagged ORF Clone

CAT#: MG216685

Trim36 (tGFP-tagged) - Mouse tripartite motif-containing 36 (Trim36) transcript variant 2, (10ug)

Product Images

Circular map for MG216685

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Trim36
• Synonyms: D18Wsu100e; haprin
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216685 representing NM_001170855
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGGATGCCTCAGACTCTCAGGTGACTATTAAGAATATCGAAAAGGAGCTTATTTGCCCAGCGT
  GCAAGGAGCTGTTTACCCACCCGCTGATCCTTCCCTGCCAGCACAGCGTCTGTCATAAGTGTGTGAAAGA
  ACTTTTACTGTCTCTTGATGACTCGTTCAATGATGTGGCGTCAGACAGCTCAAATCAGAGCAGCCCTAGG
  CTCCGGCTCACCTCCCCTAGCATGGATAAAATCGACAAGATTAACAGACCAGGCTGGAAGCGTAATTCAC
  TGACCCCGAGACCAACCACGTTCCCTTGTCCTGGCTGTGAACATGATGTGGATCTTGGAGAGCGAGGAGT
  CAGTGGTCTGTTTCGAAATTTCACTTTGGAAACGATTGTCGAGAGATACCGGCAGGCCGCTAGGGCAGCC
  ACAGCCATTATGTGTGACCTTTGTAAACCTCCACCCCAGGAATCTACAAAGAGCTGCATGGATTGTAGTG
  CAAGCTACTGCAATGAATGCTTCAAAATTTATCATCCCTGGGGCACTGTAAAAGCCCAGCATGAGTATGT
  GGGCCCCACCACTAATTTCAGACCCAAGGTTCTAATGTGCCCAGAACATGAGACAGAGAGAATAAACATG
  TACTGTGAACTATGCAGAAGGCCAGTTTGCCACCTCTGTAAGTTGGGTGGGAATCATTCCAATCACCGGG
  TAACCACCATGAGCAGTGCCTACAAAACCTTAAAGGAGAAACTTTCAAAAGACATTGATTTCCTTATTGG
  CAAGGAAAGCCAGGTGAAGAGTCAAATTTCTGAACTAAACTTGCTAATGAAAGAGACAGAGTGCAACGGA
  GAGAGGGCGAAGGAAGAAGCGCTGGCGCATTTTGAAAAGCTCTTTGAAATCCTGGAAGACAGGAAGTCGT
  CTGTTCTGAAAGCCATAGATGCCTCTAAGAAACTAAGACTAGACAAGTTTCACACTCAGATGGAAGAGTA
  CCAAGGCCTTCTAGAGAATAACGGGCTCGTGGGGTATGCTCAGGAAGTGCTGAAGGAGACGGATCAGTCT
  TGCTTTGTGCAGACGGCGAAGCAGCTCCATCTCAGAATACAGAAAGCTACGGAGTCCCTGAAGAGCTTTA
  GACCTGCAGCCCAGGCTTCTTTTGAAGACTATGTTGTTAACATATCGAAACAAACAGAGGTGCTTGGAGA
  GTTGTCCTTTTTCTCCAGTGGCATAGACATTCCTGAGATCAACGAGGAACAGAGTAAAGTGTATAATAAC
  GCCTTGATAGACTGGCATCATCCAGAAAAGGACAAAGCCGACAGCTATGTTCTAGAATACCGCAAGATTA
  ATAGAGACGAAGAAATGATATCATGGAATGAGATAGAAGTTCACGGCACAAGTAAAGTTGTCTCCAACCT
  TGAAAGCAACAGTCCCTATGCGTTCCGAGTGAGAGCTTACAGGGGTTCTATCTGCAGTCCCTGCAGCAGA
  GAATTGATCCTGCATACTCCTCCAGCTCCAGTTTTCAGTTTCCTGTTCGATGAGAAGTGTGGCTACAACA
  CTGAGCACCTCTTGCTGAACCTGAAGAGAGACCGGGTGGAGAGCAGAGCTGGATTTAACGTCCTCCTGGC
  TGCGGAGCGCATCCAAGTGGGCCATTACACAAGCTTAGACTACATCATCGGGGATGTCGGAGTCACGAAA
  GGTAAACACTTCTGGGCCTGCCGCGTGGAACCGTATTCATACCTGGTGAAAGTGGGAGTTGCTTCCAGCG
  ACAAACTGCAAGAGTGGCTGCGCTCTCCCCGAGATGCAGCTAGTCCAAGATATGAGCAAGACAGTGGACA
  TGACAGTGGAAGCGAGGACGCCTGTTTTGATTCTTCACAGCCCTTTACATTAGTTACTATAGGCATGAAG
  AAATTTTTTATACCCAAGTCACCTACTTCCTCTAATGAACCAGAAAACAGAGTTCTCCCCATGCCAACGA
  GTATAGGGATTTTCCTTGACTGTGATAAAGGCAAAGTGAGCTTCTATGATATGGACCACATGAAATGCCT
  GTATGAGCGCCAGGTGGACTGTTCGCATACAATGTATCCGGCCTTTGCCTTGATGGGCAGCGGAGGAATT
  CAGCTTGAGGAAGCCATCACAGCAAAGTATCTGGAATATGAAGAGGATGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216685 representing NM_001170855
  Red=Cloning site Green=Tags(s)
  
  MEGDASDSQVTIKNIEKELICPACKELFTHPLILPCQHSVCHKCVKELLLSLDDSFNDVASDSSNQSSPR
  LRLTSPSMDKIDKINRPGWKRNSLTPRPTTFPCPGCEHDVDLGERGVSGLFRNFTLETIVERYRQAARAA
  TAIMCDLCKPPPQESTKSCMDCSASYCNECFKIYHPWGTVKAQHEYVGPTTNFRPKVLMCPEHETERINM
  YCELCRRPVCHLCKLGGNHSNHRVTTMSSAYKTLKEKLSKDIDFLIGKESQVKSQISELNLLMKETECNG
  ERAKEEALAHFEKLFEILEDRKSSVLKAIDASKKLRLDKFHTQMEEYQGLLENNGLVGYAQEVLKETDQS
  CFVQTAKQLHLRIQKATESLKSFRPAAQASFEDYVVNISKQTEVLGELSFFSSGIDIPEINEEQSKVYNN
  ALIDWHHPEKDKADSYVLEYRKINRDEEMISWNEIEVHGTSKVVSNLESNSPYAFRVRAYRGSICSPCSR
  ELILHTPPAPVFSFLFDEKCGYNTEHLLLNLKRDRVESRAGFNVLLAAERIQVGHYTSLDYIIGDVGVTK
  GKHFWACRVEPYSYLVKVGVASSDKLQEWLRSPRDAASPRYEQDSGHDSGSEDACFDSSQPFTLVTIGMK
  KFFIPKSPTSSNEPENRVLPMPTSIGIFLDCDKGKVSFYDMDHMKCLYERQVDCSHTMYPAFALMGSGGI
  QLEEAITAKYLEYEEDV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170855
• ORF Size: 2151 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170855.1, NP_001164326.1
• RefSeq Size: 4555 bp
• RefSeq ORF: 2154 bp
• Locus ID: 28105
• UniProt ID: Q80WG7
• Cytogenetics: 18 24.41 cM
• Gene Summary: E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:19232519). Involved in chromosome segregation and cell cycle regulation (PubMed:19232519). May play a role in the acrosome reaction and fertilization (PubMed:12917430).[UniProtKB/Swiss-Prot Function]