Shroom2 (NM_172441) Mouse Tagged ORF Clone

CAT#: MG216509

Shroom2 (tGFP-tagged) - Mouse shroom family member 2 (Shroom2), (10ug)

Product Images

Circular map for MG216509

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Shroom2
• Synonyms: 4832440C16; Apxl; C630003H05Rik; Shrm2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216509 representing NM_172441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGCGCCGAGCCCCGTGCACGGCCCGAGCGCCTGGCCGAAGCTGAGGCGCCGGCGACCGACGGCG
  TCCGCCTGGTGGAGGTGCAGCTGAGCGGCGGCGCCCCTTGGGGCTTCACCTTGAAGGGCGGCCGCGAGCA
  TGGCGAGCCTCTGGTCATCACCAAGATTGAAGAAGGAAGCAAAGCTGCAGCAGTGGACAAGCTACTTGCT
  GGAGATGAGATAGTGGCCATTAATGATGTGAGTCTCTCAGGGTTCAGACAAGAAGCAATTTGCCTGGTGA
  AAGGCTCCCACAAGACCCTGAAGCTTGTGGTCAAAAGGAAAAGTGATCCAAGTTGGAGGCCCCACTCCTG
  GCACGCTACCAAGTACTTTGATGTCCACCCTGAGCCAGCAGCCTCACTGTTTCTGAACACCAGCGGTTCC
  CCTTCCTGGAAGAGCCAGCACCAGGCTAGTTCTTCCTCTCATGATTTATCTGGCTCATGGGAGCATACAA
  GCCTACAGCGCACTTCTGACCACTTCAGCTCCATGGGCAGCATAGACAGCCTCGACCACAGCTCCCAACT
  CTACCCATCTGGACACCTCTCATCTGCCAAGTCCAATAGCAGCATTGACCACCTGGGAGGCCACAGCAAG
  CGAGACTCAGCTTACGGCTCCTTTTCCACATGCTCCAGCACCCCTGACCACACCTTGCCCAAGGCTGATG
  CCTCTTCCACTGAGAACATTCTCTATAAAGTTGGCCTTTGGGAGGCCTCCAGACCAGGCAGCAGCCGACA
  AAGCCAGTCTACAGGTGATCCTCAGGGACTGCAGGACAGGCCATCATGTTTCATACCTAGGGTTCCTGGT
  AACAGTAGTAAAAGTCCAAGGCCGGAGGATAATGTTGAACCCAAAATAGCCACTCATGGGAGATCCAATT
  TTGGGCCTGTGTGGTATGTTCCTGACAAGAAAAAAGCCCCTTCTCCCCCACCTCTTGGACTTCCTTTGCG
  CAGTGATAGCTTTTCTGTGGCAGCCAGGGGCCATGAAAAAGCCCGGGGCCCTCCATTCTCAGACTTGGCC
  AGTATGCAGCACTTTATCACCCTGCCCCATGTGCAACCCCGGGGGGACCATAGAATGGAAACTACAGATC
  GTCAGTGGAAGCTCACACACCTAAGCAGTGGAAAAGAAATAGGAAATGTGGGTTACCAGTCAGAAGGCCA
  CCTGGATTGCCGTTGGCTATGCTCTGATGATAGAGCAGGTAGGCCCTCAGGGCCTCCGGGCAGGCTCCAG
  TTCTCCGATGTGCACTTTCTGAAGTCTTACCACGGGAGCCAGCACCAACAGCAGTGCAGTGACGAGAGCC
  CAAGGGCCCCCTCATCACCAAGGGAACTGCTTCATATAACTCCTGGTGGCGGTCTGCAAGAGCCTCCTGA
  ACCATCTCAGGATGACAACCCTACTCAGGTGAGGTGGCCTGGTTCTGCTCACCAGAAGCTAGATGACAGA
  GGGCGGAGCCACTATTTTCCTGGGTCCCTCAGGCAGCCTGTACAGGGGAGTGCCCAGGTTGTGATACCCC
  GAGGTGACTATTGGCATTCAGATACAACTCCTGTGGACCTTGAATATCCTCTCTTACGCCCAGTAGGACA
  GAGGACCTACCTACAGCAGCATGAGGAGACTCCGGCCTCTCATGAAAAAGAGGGGTATCACCAGCTAAAT
  GCAGGGATTGAAGGCTGCTGCTCTGGAATCCAAGAGCCTCCTAGAGCCAGCCGCACTGTGAGAACTGGTC
  TGCAGTGTCCTAGCAATGACTTCAAATTAGTGGATGGAGAGAGTGGGAGAATTTCTCGTCAGAGGACACC
  CATGCTGCATTCTCTGACCCAAGATGGTACATGGAGACCCGGGAACAGCAAGGATTGTGGAAATGATAAG
  CCACCACTGTTTGATGCCCAGGTGGGTAAACCCACACGGAGGAGTGACCGTTTTGCCACAACACTGAGGA
  ATGAGATCCAAATGCGTAGAGCAAAGCTGCAGAAGAGCAAAAGTACAGTGACACTAGCTGGAGACAGTGA
  AGCTGAAGATTGTGCTGGAGACTGGAGAGCTGATGTGGGGGCTGTCCCAGAAGGTTCCTTCCCCAGCACC
  TATAAAGAGCACCTGAAGGAGGCCCAGACACGTGTTCTGAAGGCCACCTCTTTCCAACGCCGAGATTTAG
  ATCCCACCCCAGCAGATCAGTATTCAGGACCATCAGAACACAGGACTTTTGACCACAGTGCCTCATCTTC
  TTTATCTTCTTTCCCTGGGGAGCCAGACTCTGCCCCACGCTTCTGTGAGACAGGTCTGGCCAAGGCACCC
  TCTTCTGGAGTTGGTGTACCCCACGTTCTTCGAATTGGAGGCCGGAAACGGTTCACAGCAGAGCAGAAAC
  TAAAGTCCTATTCTGAGCCAGAGAAAATAAATGAAGTGGGGCTCTCAGGGGACCACCGTCCTCATCCTAC
  TGTCAGGACACCTGAGGATACAGTGGGTACATTTGCTGACAGGTGGAAGTTTTTTGAGGAAACAAGCAAA
  TCTCTTCTCCAGAAGGCAGGCCATAGGCAAGTTCACTGTGGGCTCCCTAGAGAGAAGGCTGAGAGGCCAC
  AGACAGGGCACCATGAATGTGAGAGTACAGAGCCCTGGTTCCAGAAGAGGTCACTGGCCACCTCCTGCGG
  AGAGATCCTCAGTGATAGAAAAGTAGAAAAGGCCTCAGAGAAATTGAACCCACCCAGAAGGCTTGGAACC
  TTCGCAGAATATCAAGCATCTTGGAAGGAACAGAAGAAACCTCTGGAAGCCAGGAGTTCTGGACGATACC
  ATTCAGCAGATGATATTCTGGATGCTGGTCTAGATCAGCAGCAGAGGCCACAGTACATTCATGAGCGGTC
  TCGTTCATCACCGTCCACAGATCACTACTCACAGGAAGTGCCTGTTGAACCAAACAGGCAGGCAGAGGAC
  TCTGGTGACCACAAAGAAGCAATTCTCTGTACACTACAAGCTGAGGAGGGATGCTCTGCTCCAAGCTCCT
  CTGTGCTCAGCAGTGCCCAGCCGCAAGACAGCCAGCATGTGAATGAAGACACAACTTTCCCTCAACCAGA
  AACCCAGCTCTCTTCCAAGTGTCAACACCTACAGACGTCAGCCATGGAAACTTCTCGCTCCCCTTCGCCT
  CAGTTTGCCCCACAGAAGCTGACAGATAAACCTCCCCTGCTTATCCATGAAGACAACTCAGCAAGAATCG
  AGCGGGTGATGGACAACAACACCACTGTGAAGATGGTGCCCATAAAAATTGTGCACTCAGAAAGCCAGCC
  CGAGAAGGAGAGTCGTCAGAGTCTCGCGTGCCCAGCTGAGCTGCCCCCACTGCCCAGTGGGCTGGAGAGG
  GACCAGATCAAGACACTGAGTACATCAGAGCAATGCTACTCCCGCTTCTGTGTGTACACACGACAGGAGG
  TGGAAGCTCCTCATAGAGCCCGCCCTCCAGAGCCCCGGCCACCCAGCACGCCTGCACCTCCTGTCAGAGA
  TAGCTGTTCCTCCCCTCCCTCACTCAACTATGGGAAGGCCAAGGAGAAAACCATGGATGACTTGAAGTCT
  GAAGAATTAGCCAGGGAGATTGTGGGAAAGGACAAGTCTTTGGCTGACATCCTGGACCCCAGTGTGAAGA
  TCAAAACTACCATGGATCTGATGGAGGGAATTTTTCCCAAAGATGAGTACCTCCTAGAGGAAGCTCAGCA
  GCGGAGAAAGCTGCTCCCCAAAGTCCCCTTACCCAGAGTCACAGAGGACAAGAAACAGGACCCAGGTATG
  CCAGGGGTTGTGTCCTTGGCCACCAATTCTACCTATTACAGCACATCAGCCCCCAAAGCAGAGCTTCTTA
  TCAAGATGAAGGACCTGCAGGAGCCTGAAGAGTATTCAGCAGGTGACTTGGATCATGACCTTTCTGTTAA
  GAAGCAAGAGCTCATTGACAGTATCAGCCGCAAGCTGCAGGTGCTCCGGGAAGCACGTGAGAGCCTGCTG
  GAAGACATCCAAGCCAACAATGCTCTTGGGGATGAGGTGGAAGCCATTGTGAAAGATGTCTGCAAGCCCA
  ATGAGTTTGACAAGTTCCGGATGTTCATTGGAGACCTGGACAAAGTGGTGAACCTCCTGCTGTCACTGTC
  AGGACGCCTGGCCCGTGTGGAAAATGCCCTTAATAATTTAGATGACAATCCTTCTCCTGGAGATCGGCAG
  TCACTGTTGGAGAAACAGAGAGTCCTAACTCAGCAGCACGAGGATGCCAAGGAGCTTAAAGAGAACCTGG
  ACCGCCGTGAGCGCATTGTGTTCGACATCCTGGCTACCTACCTCAGCGAGGAGAACCTGGCTGACTATGA
  GCACTTCGTGAAGATGAAGTCAGCTCTCATCATTGAGCAGCGAGAGCTGGAAGATAAAATCCACCTGGGT
  GAAGAGCAGCTCAAGTGTTTGTTTGACAGCCTACAGCCTGAGAGAAGCAAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216509 representing NM_172441
  Red=Cloning site Green=Tags(s)
  
  MEGAEPRARPERLAEAEAPATDGVRLVEVQLSGGAPWGFTLKGGREHGEPLVITKIEEGSKAAAVDKLLA
  GDEIVAINDVSLSGFRQEAICLVKGSHKTLKLVVKRKSDPSWRPHSWHATKYFDVHPEPAASLFLNTSGS
  PSWKSQHQASSSSHDLSGSWEHTSLQRTSDHFSSMGSIDSLDHSSQLYPSGHLSSAKSNSSIDHLGGHSK
  RDSAYGSFSTCSSTPDHTLPKADASSTENILYKVGLWEASRPGSSRQSQSTGDPQGLQDRPSCFIPRVPG
  NSSKSPRPEDNVEPKIATHGRSNFGPVWYVPDKKKAPSPPPLGLPLRSDSFSVAARGHEKARGPPFSDLA
  SMQHFITLPHVQPRGDHRMETTDRQWKLTHLSSGKEIGNVGYQSEGHLDCRWLCSDDRAGRPSGPPGRLQ
  FSDVHFLKSYHGSQHQQQCSDESPRAPSSPRELLHITPGGGLQEPPEPSQDDNPTQVRWPGSAHQKLDDR
  GRSHYFPGSLRQPVQGSAQVVIPRGDYWHSDTTPVDLEYPLLRPVGQRTYLQQHEETPASHEKEGYHQLN
  AGIEGCCSGIQEPPRASRTVRTGLQCPSNDFKLVDGESGRISRQRTPMLHSLTQDGTWRPGNSKDCGNDK
  PPLFDAQVGKPTRRSDRFATTLRNEIQMRRAKLQKSKSTVTLAGDSEAEDCAGDWRADVGAVPEGSFPST
  YKEHLKEAQTRVLKATSFQRRDLDPTPADQYSGPSEHRTFDHSASSSLSSFPGEPDSAPRFCETGLAKAP
  SSGVGVPHVLRIGGRKRFTAEQKLKSYSEPEKINEVGLSGDHRPHPTVRTPEDTVGTFADRWKFFEETSK
  SLLQKAGHRQVHCGLPREKAERPQTGHHECESTEPWFQKRSLATSCGEILSDRKVEKASEKLNPPRRLGT
  FAEYQASWKEQKKPLEARSSGRYHSADDILDAGLDQQQRPQYIHERSRSSPSTDHYSQEVPVEPNRQAED
  SGDHKEAILCTLQAEEGCSAPSSSVLSSAQPQDSQHVNEDTTFPQPETQLSSKCQHLQTSAMETSRSPSP
  QFAPQKLTDKPPLLIHEDNSARIERVMDNNTTVKMVPIKIVHSESQPEKESRQSLACPAELPPLPSGLER
  DQIKTLSTSEQCYSRFCVYTRQEVEAPHRARPPEPRPPSTPAPPVRDSCSSPPSLNYGKAKEKTMDDLKS
  EELAREIVGKDKSLADILDPSVKIKTTMDLMEGIFPKDEYLLEEAQQRRKLLPKVPLPRVTEDKKQDPGM
  PGVVSLATNSTYYSTSAPKAELLIKMKDLQEPEEYSAGDLDHDLSVKKQELIDSISRKLQVLREARESLL
  EDIQANNALGDEVEAIVKDVCKPNEFDKFRMFIGDLDKVVNLLLSLSGRLARVENALNNLDDNPSPGDRQ
  SLLEKQRVLTQQHEDAKELKENLDRRERIVFDILATYLSEENLADYEHFVKMKSALIIEQRELEDKIHLG
  EEQLKCLFDSLQPERSK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172441
• ORF Size: 4461 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172441.3, NP_766029.2
• RefSeq Size: 7494 bp
• RefSeq ORF: 4464 bp
• Locus ID: 110380
• UniProt ID: A2ALU4
• Cytogenetics: X 68.46 cM
• Gene Summary: May be involved in endothelial cell morphology changes during cell spreading. In the retinal pigment epithelium, may regulate the biogenesis of melanosomes and promote their association with the apical cell surface by inducing gamma-tubulin redistribution.[UniProtKB/Swiss-Prot Function]