Itsn2 (NM_011365) Mouse Tagged ORF Clone

CAT#: MG216482

Itsn2 (tGFP-tagged) - Mouse intersectin 2 (Itsn2), (10ug)

Product Images

Circular map for MG216482

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Itsn2
• Synonyms: AI327390; Ese2; mKIAA1256; Sh3d1B; Sh3p18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216482 representing NM_011365
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGGCTCAGTTTCCCACAGCGATGAATGGAGGGCCAAATATGTGGGCTATTACATCTGAAGAACGTA
  CTAAGCATGATAAACAGTTTGATAACCTCAAACCTTCAGGAGGTTACATAACAGGTGATCAAGCCCGTAC
  TTTTTTCCTACAGTCAGGTCTGCCGGCCCCGGTTTTAGCTGAAATATGGGCCTTATCAGATCTGAACAAG
  GATGGGAAGATGGACCAGCAAGAGTTCTCTATAGCTATGAAACTCATCAAGTTAAAGTTGCAGGGCCAAC
  AGCTGCCTGTAGTCCTCCCTCCTATCATGAAACAACCCCCTATGTTCTCTCCACTAATCTCTGCTCGTTT
  TGGGATGGGAAGCATGCCCAATCTGTCCATTCATCAGCCATTGCCTCCAGTTGCACCTATAGCAACACCC
  TTGTCTTCTGCTACTTCAGGGACCAGTATTCCTCCCCTAATGATGCCTGCTCCCCTAGTGCCTTCTGTTA
  GTACATCCTCATTACCAAATGGAACTGCCAGTCTCATTCAGCCTTTATCCATTCCTTATTCTTCTTCAAC
  ATTGCCTCATGCATCATCTTACAGCCTGATGATGGGAGGATTTGGTGGTGCTAGTATCCAGAAGGCCCAG
  TCTCTGATTGATTTAGGATCTAGTAGCTCAACTTCCTCAACTGCTTCCCTCTCAGGGAACTCACCTAAGA
  CAGGGACCTCAGAGTGGGCAGTTCCTCAGCCTTCAAGATTAAAGTATCGGCAAAAATTTAATAGTCTAGA
  CAAAGGCATGAGCGGATACCTCTCAGGTTTTCAAGCTAGAAATGCCCTTCTTCAGTCAAATCTCTCTCAA
  ACTCAGCTAGCTACTATTTGGACTCTGGCTGACATCGATGGTGACGGACAGTTGAAAGCTGAAGAATTTA
  TTCTGGCGATGCACCTCACTGACATGGCCAAAGCTGGACAGCCACTACCACTGACGTTGCCTCCCGAGCT
  TGTCCCTCCATCTTTCAGAGGGGGAAAGCAAGTTGATTCTGTTAATGGAACTCTGCCTTCATATCAGAAA
  ACACAAGAAGAAGAGCCTCAGAAGAAACTGCCAGTTACTTTTGAGGACAAACGGAAAGCCAACTATGAAC
  GAGGAAACATGGAGCTGGAGAAGCGACGCCAAGTGTTGATGGAGCAGCAGCAGAGGGAGGCTGAACGCAA
  AGCCCAGAAAGAGAAGGAAGAGTGGGAGCGGAAACAGAGAGAACTGCAAGAGCAAGAATGGAAGAAGCAG
  CTGGAGTTGGAGAAACGCTTGGAGAAACAGAGAGAGCTGGAGAGACAGCGGGAGGAAGAGAGGAGAAAGG
  AGATAGAAAGACGAGAGGCAGCAAAACAGGAGCTTGAGAGACAACGCCGTTTAGAATGGGAAAGACTCCG
  TCGGCAGGAGCTGCTCAGTCAGAAGACCAGGGAACAAGAAGACATTGTCAGGCTGAGCTCCAGAAAGAAA
  AGTCTCCACCTGGAACTGGAAGCAGTGAATGGAAAACATCAGCAGATCTCAGGCAGACTACAAGATGTCC
  AAATCAGAAAGCAAACACAAAAGACTGAGCTAGAAGTTTTGGATAAACAGTGTGACCTGGAAATTATGGA
  AATCAAACAACTTCAACAAGAGCTTAAGGAATATCAAAATAAGCTTATCTATCTGGTCCCTGAGAAGCAG
  CTATTAAACGAAAGAATTAAAAACATGCAGCTCAGTAACACACCTGATTCAGGGATCAGTTTACTTCATA
  AAAAGTCATCAGAAAAGGAAGAATTATGCCAAAGACTTAAAGAACAATTAGATGCTCTTGAAAAAGAAAC
  TGCATCTAAGCTCTCAGAAATGGATTCATTTAACAATCAGCTGAAGGAACTCAGAGAAAGCTATAATACA
  CAGCAGTTAGCCCTTGAACAACTTCATAAAATCAAACGTGACAAATTGAAGGAAATCGAAAGAAAAAGAT
  TAGAGCAAATTCAAAAAAAGAAACTAGAAGATGAGGCTGCAAGGAAAGCAAAGCAAGGAAAAGAAAACTT
  GTGGAGAGAAAGTATTAGAAAGGAAGAAGAGGAAAAGCAAAAACGACTCCAGGAAGAAAAGTCACAGGAC
  AAAACTCAAGAAGAGGAACGAAAAGCTGAGGCAAAACAAAGTGAGACAGCCAGTGCTTTGGTGAATTACA
  GAGCACTGTACCCTTTTGAAGCAAGAAACCATGATGAGATGAGTTTTAGTTCTGGGGATATAATTCAGGT
  TGATGAAAAAACTGTAGGAGAGCCTGGTTGGCTTTATGGTAGTTTTCAGGGAAAGTTTGGCTGGTTCCCG
  TGCAACTATGTAGAAAAAGTGCTGTCAAGTGAAAAAGCTCTGTCTCCTAAGAAGGCCTTACTTCCTCCTA
  CAGTGTCTCTCTCTGCTACCTCAACTTCTTCCCAGCCACCAGCATCAGTGACTGATTATCACAATGTATC
  CTTCTCAAACCTTACTGTTAATACAACATGGCAGCAGAAGTCAGCTTTTACCCGCACTGTGTCCCCTGGA
  TCTGTGTCCCCCATTCACGGACAGGGGCAGGCTGTAGAAAACCTGAAAGCCCAGGCCCTTTGTTCCTGGA
  CGGCAAAGAAGGAGAACCACCTGAACTTCTCAAAGCACGACGTCATCACTGTCCTGGAGCAGCAGGAAAA
  CTGGTGGTTTGGGGAGGTGCACGGAGGAAGAGGATGGTTCCCCAAGTCTTATGTCAAGCTCATTCCTGGG
  AATGAAGTACAGCGAGGAGAGCCAGAAGCTTTGTATGCAGCTGTGACTAAGAAACCTACCTCCACAGCCT
  ATCCAGTTACCTCCACAGCCTATCCAGTTGGAGAAGACTACATTGCACTTTATTCATACTCAAGTGTAGA
  GCCCGGGGATTTGACTTTCACTGAAGGTGAAGAAATTCTAGTGACCCAGAAAGATGGAGAGTGGTGGACA
  GGAAGTATTGGAGAGAGAACTGGAATCTTCCCGTCCAACTACGTCAGACCAAAGGATCAAGAGAATTTTG
  GGAATGCTAGCAAATCTGGAGCATCAAACAAAAAACCCGAGATCGCTCAAGTAACTTCAGCATATGCTGC
  TTCAGGGACTGAGCAGCTCAGCCTTGCGCCAGGACAGTTAATATTAATCTTAAAGAAAAACACAAGCGGG
  TGGTGGCAAGGAGAGCTACAGGCCAGAGGGAAGAAACGACAGAAGGGATGGTTTCCTGCCAGCCATGTAA
  AGCTGCTAGGTCCAAGCAGTGAAAGAACCATGCCTACTTTTCACGCTGTATGTCAAGTGATTGCTATGTA
  TGACTACATGGCGAATAACGAAGATGAGCTCAATTTCTCCAAAGGACAGCTGATTAATGTTATGAACAAA
  GATGACCCTGACTGGTGGCAAGGAGAAACCAATGGTCTGACTGGTCTCTTTCCTTCAAACTATGTTAAGA
  TGACAACAGACTCAGATCCAAGTCAACAGTGGTGTGCTGACCTCCAAGCCCTGGACACAATGCAGCCTAC
  GGAGAGGAAGCGACAGGGCTACATTCACGAGCTCATTCAGACAGAGGAGCGGTACATGGACGACCTGCAG
  CTGGTCATCGAGGTCTTCCAGAAACGGATGGCTGAGTCAGGCTTCCTCACTGAAGCAGACATGGCTCTGA
  TCTTTGTGAACTGGAAAGAGCTCATCATGTCCAACACGAAGCTGCTGAGGGCCTTGCGGGTGAGGAAGAA
  GACTGGGGGTGAGAAGATGCCAGTTCAGATGATTGGAGACATCCTGGCGGCAGAGCTGTCCCACATGCAG
  GCCTACATCCGCTTCTGCAGCTGTCAGCTTAATGGGGCAACCCTGTTACAGCAGAAGACAGACGAGGACA
  CGGACTTCAAGGAATTTCTAAAGAAGTTGGCATCAGACCCACGATGCAAAGGGATGCCCCTCTCCAGCTT
  CCTGCTGAAGCCCATGCAGAGGATCACTCGCTACCCGCTGCTCATCCGAAGTATCCTGGAGAACACTCCA
  CAGAGTCATGTTGACCACTCCTCCCTGAAGCTGGCCCTAGAACGTGCTGAGGAGCTGTGCTCTCAGGTGA
  ACGAGGGAGTCCGGGAGAAGGAAAATTCAGACCGGCTGGAGTGGATCCAGGCACACGTGCAGTGCGAAGG
  CTTGGCAGAGCAACTTATTTTCAACTCCCTCACCAACTGCCTGGGCCCCCGGAAGCTTCTGCACAGCGGG
  AAGCTGTACAAGACCAAGAGCAATAAGGAGCTGCACGCCTTCCTCTTCAACGACTTCCTGCTGCTCACCT
  ACCTGGTCAGGCAGTTTGCCGCCGCCTCTGGCCACGAGAAGCTCTTCAACTCCAAGTCCAGTGCTCAGTT
  CCGGATGTACAAAACGCCCATTTTCCTGAATGAAGTGTTGGTGAAACTTCCCACAGACCCTTCCAGCGAT
  GAGCCCGTCTTCCACATTTCCCACATTGATCGTGTGTACACACTCCGAACAGACAACATCAACGAGAGGA
  CGGCCTGGGTCCAGAAGATCAAGGGTGCCTCAGAGCAGTACATCGACACTGAGAAGAAGAAACGGGAAAA
  GGCTTACCAAGCCCGTTCTCAAAAGACTTCAGGTATTGGGCGTCTGATGGTGCATGTCATTGAAGCTACA
  GAATTAAAAGCCTGCAAACCAAACGGGAAAAGTAATCCATACTGTGAAGTCAGCATGGGCTCCCAAAGCT
  ATACCACCAGGACCCTGCAGGACACACTAAACCCCAAGTGGAACTTCAACTGCCAGTTCTTCATCAAGGA
  TCTTTACCAGGACGTTCTGTGTCTCACTATGTTTGACAGAGACCAGTTTTCTCCAGATGACTTCTTGGGT
  CGTACTGAAGTTCCAGTGGCAAAAATCCGAACAGAACAGGAAAGCAAAGGCCCCACCACCCGCCGACTAC
  TACTGCACGAAGTCCCCACTGGAGAAGTCTGGGTCCGCTTTGACCTGCAACTTTTTGAACAAAAAACTCT
  CCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216482 representing NM_011365
  Red=Cloning site Green=Tags(s)
  
  MMAQFPTAMNGGPNMWAITSEERTKHDKQFDNLKPSGGYITGDQARTFFLQSGLPAPVLAEIWALSDLNK
  DGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIHQPLPPVAPIATP
  LSSATSGTSIPPLMMPAPLVPSVSTSSLPNGTASLIQPLSIPYSSSTLPHASSYSLMMGGFGGASIQKAQ
  SLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPSRLKYRQKFNSLDKGMSGYLSGFQARNALLQSNLSQ
  TQLATIWTLADIDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQVDSVNGTLPSYQK
  TQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQVLMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQ
  LELEKRLEKQRELERQREEERRKEIERREAAKQELERQRRLEWERLRRQELLSQKTREQEDIVRLSSRKK
  SLHLELEAVNGKHQQISGRLQDVQIRKQTQKTELEVLDKQCDLEIMEIKQLQQELKEYQNKLIYLVPEKQ
  LLNERIKNMQLSNTPDSGISLLHKKSSEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKELRESYNT
  QQLALEQLHKIKRDKLKEIERKRLEQIQKKKLEDEAARKAKQGKENLWRESIRKEEEEKQKRLQEEKSQD
  KTQEEERKAEAKQSETASALVNYRALYPFEARNHDEMSFSSGDIIQVDEKTVGEPGWLYGSFQGKFGWFP
  CNYVEKVLSSEKALSPKKALLPPTVSLSATSTSSQPPASVTDYHNVSFSNLTVNTTWQQKSAFTRTVSPG
  SVSPIHGQGQAVENLKAQALCSWTAKKENHLNFSKHDVITVLEQQENWWFGEVHGGRGWFPKSYVKLIPG
  NEVQRGEPEALYAAVTKKPTSTAYPVTSTAYPVGEDYIALYSYSSVEPGDLTFTEGEEILVTQKDGEWWT
  GSIGERTGIFPSNYVRPKDQENFGNASKSGASNKKPEIAQVTSAYAASGTEQLSLAPGQLILILKKNTSG
  WWQGELQARGKKRQKGWFPASHVKLLGPSSERTMPTFHAVCQVIAMYDYMANNEDELNFSKGQLINVMNK
  DDPDWWQGETNGLTGLFPSNYVKMTTDSDPSQQWCADLQALDTMQPTERKRQGYIHELIQTEERYMDDLQ
  LVIEVFQKRMAESGFLTEADMALIFVNWKELIMSNTKLLRALRVRKKTGGEKMPVQMIGDILAAELSHMQ
  AYIRFCSCQLNGATLLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTP
  QSHVDHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSG
  KLYKTKSNKELHAFLFNDFLLLTYLVRQFAAASGHEKLFNSKSSAQFRMYKTPIFLNEVLVKLPTDPSSD
  EPVFHISHIDRVYTLRTDNINERTAWVQKIKGASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEAT
  ELKACKPNGKSNPYCEVSMGSQSYTTRTLQDTLNPKWNFNCQFFIKDLYQDVLCLTMFDRDQFSPDDFLG
  RTEVPVAKIRTEQESKGPTTRRLLLHEVPTGEVWVRFDLQLFEQKTLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_011365
• ORF Size: 4974 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_011365.4, NP_035495.2
• RefSeq Size: 6001 bp
• RefSeq ORF: 4977 bp
• Locus ID: 20403
• Cytogenetics: 12 A1.1