Iws1 (NM_173441) Mouse Tagged ORF Clone

CAT#: MG216295

Iws1 (tGFP-tagged) - Mouse IWS1 homolog (S. cerevisiae) (Iws1), (10ug)

Product Images

Circular map for MG216295

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Iws1
• Synonyms: 1700069O15Rik; 3000008H23; AL117927; AW214353
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216295 representing NM_173441
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTCGGAGTACTACAGCGGGGACCAGTCAGATGATGGTGGTGCAACCCCAGTACAGGACGAGCGGG
  ATTCAGGGTCAGATGGTGAGGATGGTGTAACCGAGCAGCACTCCGGATCCGACACTGGAAGTGTAGACCA
  CCACTCAGAGAATGAAACTAGTGATAGAGAAGATGGCTTGGCCAAGATACATAATGGAACAGACTCTGAG
  AATGATGAACCTTCCAATGCTAATGCCAGTGACTCTGAAAGTGAGGAGCTTCACAGGCCAAAGGACAGTG
  ACTCTGACTCTGAGGAGCATGCAGAGTCTCCTGCTAGTGATTCTGAAAATGAACCTGTCAACCAACATGG
  GAGCGACTCTGAAAATGAAGAGCTGCTTAATGGGCATGCCAGTGACTCTGAAAAGGAAGAGGTTAGCAAG
  CATGCCGCCAGTGACTCTGAAGCTGAGGACACTCTGCAGCCTCAGGTCAGCGAGTCTGACAGTGAAGATC
  CCCCAAGGCCCCAGGCCAGTGACTCAGAAAATGAGGAGCCTCCCAAACCTCGGATAAGTGACTCGGAGAG
  TGAGGAGCTCCCCAAACCCCGAGTCAGTGACTCAGAAAGTGAGGACCCTCCAAGGCCCCAGGCCAGTGAC
  TCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCTGAAAGTGAGGACCCTCCAAGGCCCCAGG
  CCAGTGACTCAGAAAGCGAGGAGCTCCCCAAACCCCGAGTCAGCGACTCGGAAAGTGAGGACCCTCAGAA
  AGGACCTGCTAGTGATTCTGAAGCTGAGGATGCCTCCAGACACAAAGAGAAGCCAGACTCAGATGACAGT
  GATGGGGAGAATAAGAGGGAAGATTCTGAAGTGCAGAATGAGTCAGATGGCCATACAGACAGAAAAGGAC
  TCCACAGCTCTGACAGCGAGGAGGAAGAACCCAAAAGGCAAAAAATTGACAGTGATGATGATGAAGAAAA
  GGAGGGGGATGAGGAGAAAGTAGCAAAGCGGAAGGCTGCTGTGCTTTCTGATAGTGAGGATGACGCAGGC
  AACGCATCAGCAAAGAAGAGTCGAGTTGTCTGTGATGCTGATGACTCTGATAGTGATGTTGTATCGGACA
  AGTCAGGCAAACGAGAGACAACAGTAGCATCTGACAGTGAAGAAGAAGCCGGGAAAGAAGAGTCTTCTGT
  GAAGAAGAAGGAAGACAAGGACCTGTTTGGGAGTGACAGTGAGTCCGGCAATGAAGAAGAAAATCTTATT
  GCAGATATATTTGGAGAATCTGGTGATGAAGAGGAAGAGGAATTTACGGGTTTTAACCAAGAAGATTTGG
  AGGAAGAGAAAAACGAAACACAGCTAAAGGAAGCAGAAGATTCTGATTCTGATGATAACATAAAGAGAGG
  CAAGCATATGGACTTTCTGTCAGATTTTGAGATGATGTTACAACGAAAAAAAAGCATGTGTGGCAAACGC
  AGACGAAATCGAGATGGGGGCACTTTTATTAGTGATGCAGATGATGTTGTGAGCGCCATGATTGTCAAGA
  TGAATGAAGCTGCTGAGGAAGACAGACAATTGAATAATCAAAAAAAGCCAGCTCTGAAAAAATTAACCTT
  ATTGCCTACTGTGGTTATGCACCTTAAAAAGCAGGACCTTAAAGAAACATTTATTGACAGTGGTGTGATG
  TCTGCCATTAAAGAATGGCTCTCACCTCTACCAGATAGGAGTTTGCCTGCACTGAAGATTCGGGAGGAAT
  TGTTAAAGATTCTGCAAGAGCTACCTAGTGTGAGCCAAGAGACCCTGAAGCATAGTGGGATTGGCCGAGC
  AGTGATGTATCTGTATAAACACCCTAAGGAATCAAGGTCCAACAAGGATATGGCAGGGAAGCTCATCAAT
  GAGTGGTCTCGGCCCATATTTGGTCTTACCTCAAACTATAAAGGAATGACAAGAGAAGAAAGGGAACAGA
  GAGATCTAGAACAAATGCCACAGCGACGAAGATTGAGCAGCACTGGTGGTCAGACACCACGAAGAGACCT
  GGAGAAGGTCCTAACAGGAGAAGAAAAGGCTCTTAGACCTGGAGATCCTGGCTTCTGTGCCCGTGCAAGA
  GTCCCAATGCCATCAAACAAGGACTATGTTGTCAGGCCCAAGTGGAATGTAGAAATGGAGTCATCCAGGT
  TTCAGGCATCCTCCAAGAAAGGTATCAGTCGACTGGATAAACAGATGAGAAAGTTCACAGATATCAGGAA
  AAAAAGCAGATCTGCACATGCAGTGAAAATCAGCATCGAGGGCAATAAAATGCCATTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216295 representing NM_173441
  Red=Cloning site Green=Tags(s)
  
  MDSEYYSGDQSDDGGATPVQDERDSGSDGEDGVTEQHSGSDTGSVDHHSENETSDREDGLAKIHNGTDSE
  NDEPSNANASDSESEELHRPKDSDSDSEEHAESPASDSENEPVNQHGSDSENEELLNGHASDSEKEEVSK
  HAASDSEAEDTLQPQVSESDSEDPPRPQASDSENEEPPKPRISDSESEELPKPRVSDSESEDPPRPQASD
  SESEELPKPRVSDSESEDPPRPQASDSESEELPKPRVSDSESEDPQKGPASDSEAEDASRHKEKPDSDDS
  DGENKREDSEVQNESDGHTDRKGLHSSDSEEEEPKRQKIDSDDDEEKEGDEEKVAKRKAAVLSDSEDDAG
  NASAKKSRVVCDADDSDSDVVSDKSGKRETTVASDSEEEAGKEESSVKKKEDKDLFGSDSESGNEEENLI
  ADIFGESGDEEEEEFTGFNQEDLEEEKNETQLKEAEDSDSDDNIKRGKHMDFLSDFEMMLQRKKSMCGKR
  RRNRDGGTFISDADDVVSAMIVKMNEAAEEDRQLNNQKKPALKKLTLLPTVVMHLKKQDLKETFIDSGVM
  SAIKEWLSPLPDRSLPALKIREELLKILQELPSVSQETLKHSGIGRAVMYLYKHPKESRSNKDMAGKLIN
  EWSRPIFGLTSNYKGMTREEREQRDLEQMPQRRRLSSTGGQTPRRDLEKVLTGEEKALRPGDPGFCARAR
  VPMPSNKDYVVRPKWNVEMESSRFQASSKKGISRLDKQMRKFTDIRKKSRSAHAVKISIEGNKMPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_173441
• ORF Size: 2298 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_173441.3, NP_775617.1
• RefSeq Size: 9623 bp
• RefSeq ORF: 2301 bp
• Locus ID: 73473
• UniProt ID: Q8C1D8
• Cytogenetics: 18 B1
• Gene Summary: Transcription factor which plays a key role in defining the composition of the RNA polymerase II (RNAPII) elongation complex and in modulating the production of mature mRNA transcripts. Acts as an assembly factor to recruit various factors to the RNAPII elongation complex and is recruited to the complex via binding to the transcription elongation factor SUPT6H bound to the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A). The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2) to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription (By similarity).[UniProtKB/Swiss-Prot Function]