Pde1c (NM_001159952) Mouse Tagged ORF Clone
CAT#: MG216133
- TrueORF®
Pde1c (tGFP-tagged) - Mouse phosphodiesterase 1C (Pde1c) transcript variant 3, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001159952" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Pde1c |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216133 representing NM_001159952
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGTCTCCAACCAAGGAAATTGAAGAATTCGAGAGCAACTCTCTGAAACACCTGCAACCTGAGCAGA TCGAGAAAATCTGGCTTCGGCTCCGCGGGCTGTTACGGTCTTTGGTCAAGCAATTAGAACGAGGAGAGGC CTCTGTGGTAGACCTTAAGAAGAATCTGGAATATGCAGCCACAGTGCTTGAATCTGTATATATTGATGAA ACAAGGCGACTGCTGGATACAGAGGATGAGCTTAGTGACATTCAATCAGATGCTGTGCCTTCAGAAGTTC GGGATTGGCTGGCCTCCACCTTCACACGGCAGATGGGGATGATGCTTAGGAGAAGTGATGAGAAGCCCAG GTTCAAGAGCATCGTCCATGCGGTGCAAGCTGGGATATTTGTGGAAAGAATGTACAGACGGACATCAAAC ATGGTTGGGCTGAGCTATCCACCGGCTGTAATCGATGCATTGAAGGATGTGGATACGTGGTCCTTCGATG TCTTTTCCCTCAATGAGGCCAGTGGAGATCATGCACTGAAGTTCATTTTCTATGAATTACTCACACGTTA TGACCTGATCAGCCGTTTTAAGATACCGATTTCTGCACTTGTCTCATTTGTGGAGGCCCTCGAAGTGGGT TACAGCAAGCACAAAAATCCTTACCATAACCTGATGCATGCAGCTGACGTCACACAGACTGTGCATTACC TCCTTTATAAGACAGGAGTAGCAAACTGGCTGACAGAGCTGGAGATCTTTGCAATAATCTTTTCGGCTGC CATCCATGACTATGAACATACTGGAACTACAAACAATTTCCACATTCAGACTCGGTCAGATCCAGCTATC CTGTACAATGACAGATCTGTCCTGGAGAACCACCACCTGAGTGCAGCCTACCGCCTTCTGCAGGAAGACG AAGAGATGAATATTCTGGTTAACCTCTCAAAGGATGACTGGAGGGAGTTTCGAACTTTGGTTATTGAGAT GGTAATGGCCACAGATATGTCCTGTCATTTCCAGCAAATCAAAGCCATGAAGACAGCCCTGCAGCAACCA GAAGCAATTGAGAAGCCGAAAGCCTTATCCCTCATGTTACACACAGCAGACATCAGTCATCCTGCGAAAG CATGGGACCTGCACCACCGCTGGACCATGTCTCTCCTGGAGGAGTTCTTTAGACAGGGTGACAGAGAAGC AGAGCTGGGGCTGCCATTTTCTCCTCTTTGTGACAGAAAGTCAACCATGGTTGCTCAGTCACAAGTGGGT TTTATTGACTTCATTGTGGAGCCCACCTTCACTGTGCTCACGGACATGACCGAGAAGATTGTGAGTCCAT TAATTGACGAAAGCTCCCAGACTGGTGGGACAGGGCAGAGGAGATCAAGTTTGAACAGCATCAACTCATC AGATGCAAAGCGATCCGGTGTCAAGAGTTCTGGGTCAGATGGAAGTGCTCCCATCAACAATTCTGTCATT CCTGTTGACTATAAGAGTTTTAAAGCCACTTGGACTGAGGTGGTGCAGATCAATCGGGAGCGGTGGCGAG CCAAGGTACCCAAAGAAGAAAAAGCCAAGAAGGAAGCTGAAGAGAAGGCTCGCCTGGCTGCTGAGGAAAA GCAAAAGGAAATGGAAGCCAAAAGCCAAGCTGAACAAGGCACAACCAGCAAAGGCGAGAAAAAGACATCA GGAGAAGCCAAAAGTCAAGTCAATGGAACACGCAAGGGTGATAACCCTCGTGGGAAGAACTCCAAAGGAG AAAAGGCAGGCGAAAAGCAGCAGAACGGTGACTTGAAAGACGGTAAAAATAAGGCAGACAAGAAGGATCA CTCCAACACCGGAAATGAGTCAAAGAAAACAGATGATCCAGAGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216133 representing NM_001159952
Red=Cloning site Green=Tags(s) MESPTKEIEEFESNSLKHLQPEQIEKIWLRLRGLLRSLVKQLERGEASVVDLKKNLEYAATVLESVYIDE TRRLLDTEDELSDIQSDAVPSEVRDWLASTFTRQMGMMLRRSDEKPRFKSIVHAVQAGIFVERMYRRTSN MVGLSYPPAVIDALKDVDTWSFDVFSLNEASGDHALKFIFYELLTRYDLISRFKIPISALVSFVEALEVG YSKHKNPYHNLMHAADVTQTVHYLLYKTGVANWLTELEIFAIIFSAAIHDYEHTGTTNNFHIQTRSDPAI LYNDRSVLENHHLSAAYRLLQEDEEMNILVNLSKDDWREFRTLVIEMVMATDMSCHFQQIKAMKTALQQP EAIEKPKALSLMLHTADISHPAKAWDLHHRWTMSLLEEFFRQGDREAELGLPFSPLCDRKSTMVAQSQVG FIDFIVEPTFTVLTDMTEKIVSPLIDESSQTGGTGQRRSSLNSINSSDAKRSGVKSSGSDGSAPINNSVI PVDYKSFKATWTEVVQINRERWRAKVPKEEKAKKEAEEKARLAAEEKQKEMEAKSQAEQGTTSKGEKKTS GEAKSQVNGTRKGDNPRGKNSKGEKAGEKQQNGDLKDGKNKADKKDHSNTGNESKKTDDPEE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001159952 |
ORF Size | 1866 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001159952.1, NP_001153424.1 |
RefSeq Size | 8837 bp |
RefSeq ORF | 1869 bp |
Locus ID | 18575 |
UniProt ID | Q64338 |
Cytogenetics | 6 27.65 cM |
Gene Summary | Calmodulin-dependent cyclic nucleotide phosphodiesterase with a dual-specificity for cAMP and cGMP, which are key regulators of many important physiological processes (PubMed:8810348). Exhibits high affinity for both cAMP and cGMP (By similarity). Modulates the amplitude and duration of the cAMP signal in sensory cilia in response to odorant stimulation, hence contributing to the generation of action potentials. Regulates smooth muscle cell proliferation. Regulates the stability of growth factor receptors, including PDGFRB (Probable).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219809 | Pde1c (untagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 3, (10ug) |
USD 638.00 |
|
MR216133 | Pde1c (Myc-DDK-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 3 |
USD 637.00 |
|
MR216133L3 | Lenti ORF clone of Pde1c (Myc-DDK-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 3 |
USD 937.00 |
|
MR216133L4 | Lenti ORF clone of Pde1c (mGFP-tagged) - Mouse phosphodiesterase 1C (Pde1c), transcript variant 3 |
USD 937.00 |
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