Nosip (NM_001163684) Mouse Tagged ORF Clone
CAT#: MG216123
- TrueORF®
Nosip (tGFP-tagged) - Mouse nitric oxide synthase interacting protein (Nosip) transcript variant 2, (10ug)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001163684" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Nosip |
Synonyms | 2310061K06Rik; CGI-25 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216123 representing NM_001163684
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGCGGCATGGCAAGAACTGCACTGCAGGGGCCGTCTACACCTACCACGAGAAGAAGAAGGACACAG CGGCCTCGGGCTATGGGACCCAGAACATTCGACTGAGCCGGGACGCTGTGAAAGACTTTGACTGCTGCTG TCTCTCTCTGCAGCCCTGCCATGACCCTGTGGTCACGCCAGACGGCTACCTGTATGAGAGGGAAGCGATC CTGGAGTACATCCTGCACCAGAAGAGAGAGATCGCCCGGCAGGTGAAGGCCTATGAGAAGCAGCGAGGAG CGAGGAGAGAAGAGCAGAAAGAGCTGCAGCGAGCTGCAGCCCAGGACCAAGTTAGAGGCTTCCTAGAGAA GGAGGCGGCCATCGTGAGCCGGCCCCTCAACCCCTTCATGCCCAAGGCTGCCACCTTGCCCAACACAGAG GGTGAACAGCCAGGGCCCAGTGTGGGTCCCGTAGGCAAGGACAAGGACAAAGCACTGCCCAGCTTCTGGA TTCCCTCACTAACGCCCGAGGCAAAGGCCACCAAGCTGGAAAAGCCGCTGGACGACTCTGTGGACCGCGT GGGACTCATCACACGCAGTGAGCGCTACGTTTGCGCAGTGACCCGAGACAGTCTGAGCAATGCCACGCCG TGCGCAGTGCTGCGTCCCTCTGGGGCTGTGGTCACCCTGGAGTGTGTTGAGAAACTGATCCGGAAGGACA TGGTGGACCCCGTGAATGGGGACACGCTGACAGAGCGCGACATCATCGTGCTGCAGCGGGGCGGCACCGG CTTTGCGGGCTCCGGAGTGAAGCTACAGGCAGAAATGTCTCGGCCAGTGATGCAGGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216123 representing NM_001163684
Red=Cloning site Green=Tags(s) MTRHGKNCTAGAVYTYHEKKKDTAASGYGTQNIRLSRDAVKDFDCCCLSLQPCHDPVVTPDGYLYEREAI LEYILHQKREIARQVKAYEKQRGARREEQKELQRAAAQDQVRGFLEKEAAIVSRPLNPFMPKAATLPNTE GEQPGPSVGPVGKDKDKALPSFWIPSLTPEAKATKLEKPLDDSVDRVGLITRSERYVCAVTRDSLSNATP CAVLRPSGAVVTLECVEKLIRKDMVDPVNGDTLTERDIIVLQRGGTGFAGSGVKLQAEMSRPVMQA TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001163684 |
ORF Size | 828 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001163684.1, NP_001157156.1 |
RefSeq Size | 2059 bp |
RefSeq ORF | 831 bp |
Locus ID | 66394 |
UniProt ID | Q9D6T0 |
Cytogenetics | 7 B3 |
Gene Summary | E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (PubMed:25546391). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210358 | Nosip (untagged) - Mouse nitric oxide synthase interacting protein (Nosip), transcript variant 2, (10ug) |
USD 330.00 |
|
MR216123 | Nosip (Myc-DDK-tagged) - Mouse nitric oxide synthase interacting protein (Nosip), transcript variant 2 |
USD 330.00 |
|
MR216123L3 | Lenti ORF clone of Nosip (Myc-DDK-tagged) - Mouse nitric oxide synthase interacting protein (Nosip), transcript variant 2 |
USD 630.00 |
|
MR216123L4 | Lenti ORF clone of Nosip (mGFP-tagged) - Mouse nitric oxide synthase interacting protein (Nosip), transcript variant 2 |
USD 630.00 |
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