Cyb5r4 (NM_024195) Mouse Tagged ORF Clone

CAT#: MG216118

Cyb5r4 (tGFP-tagged) - Mouse cytochrome b5 reductase 4 (Cyb5r4), (10ug)

Product Images

Circular map for MG216118

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cyb5r4
• Synonyms: 2810034J18Rik; B5+B5R; b5/b5r; b5b5r; C79736; Cb5cb5r; Ncb5or
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG216118 representing NM_024195
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAACGTCCCTTCCCAGGCTTTCCCGGCCCCCGGCTCCCAGCAGCGCGTCTCCTCCCAGGGGCGCA
  GCAAGGTACCTTTAAAACAAGGCAGGAGTCTTATGGATTGGATCCGACTGACCAAGAGTGGAAAGGACTT
  AACAGGATTGAAGGGCGGGTTAATTGAAGTAACTGAAGAAGAGCTTAAGAAGCACAACAAGAAAGAAGAT
  TGTTGGATATGCATAAGAGGGTTTGTTTACAACGTCAGCCCTTATATGGAGTACCATCCGGGTGGGGAGG
  ATGAACTCATGAGAGCCGCAGGAGCCGATGGAACAGATCTTTTTAATGAGGTTCATCGTTGGGTCAATTA
  TGAATCCATGCTGAAAGAATGCCTGGTTGGCAGGATGGCTGTGAAGCCTGCTGTTCCAAAAGACTGTCAT
  GAAGGAAAGAGAGTCTTAAATGGCATGCTTCCCAAGAGCCAAATGTCAGACACACTGCCCAGGGATGTGA
  CAGACACACTGCCCAGGGAAGACCTCAGTTCTCCAAGCTATGATTGGTTCCAAACAGAGTCTTCAGTCAC
  CATTGTTGTATACACCAAACAAAAGAATATCAGTTTAGACTCAGTAATCGTTGACCTCCAGGATGACTCC
  TTGAGAGCAGAAGCAGTCATCAAGGATCACTCTTACCTTGTACATGTTGGGCTAAGCCATGAAGTTCAAG
  AAAATTTTTCTGTACGGGTTATTGAAAATGTTGGAAAAATAGAGATTGTCCTACAAAAGAAAGAAAGTGT
  TTCTTGGCAATGTCTTGGTGATCACCTGGAGAAACATGATTCTTTCATTCCAAAGAAAGATACAGGCTTG
  TACTACAGACGGTGCCAGTTAATATCTAAGGAAGATGTCACTCATGACACGAGACTTTTCTGTCTGATGC
  TGCCTCCAAGTACTCACCTTCAGGTGCCAGTTGGACAACATGTTTACCTCAAGCTCAGTGTCACAGGTGC
  AGAAATAGTAAAGCCATATACACCCGTGTCTGATTCCTTACTCTCAGATTTTAAAGAACCAGTCCTTTCC
  CCAAATAAATACATCTGCTTTTTGATCAAAATCTATCCTGCTGGACTCTTCACACCAGAGCTTGATCGTC
  TTCAGATTGGAGATTTTATTTCTGTAAGCGGACCTGAAGGCGATTTTAAAGTATCCAAACTCCAGGAAGT
  AGAAGATCTCTTTCTATTGGCAGCCGGAACAGGATTCACACCAATGGTCACTGTACTGAATTATGCTTTG
  TCTCACATGTCCAGTCTCAGGAAGGTGAAGTTGATGTTCTTCAATAAAACAGAGGACGATATAATTTGGA
  GATGTCAGCTAGAGAAGTTGGCATTAAGAGAAAAAAGATTTGATGTTGAATTTGTTCTCTCGGCACCTTC
  TCCAGAATGGAATGGCAAACAGGGACACATATCACGAGCTCTTCTCTCTGAATTTTTGCAAAGAAGTTCA
  GAAAACTCTAGGGCCTTCCTTTGCATCTGCGGACCAACACCATTTACTGACGAGGGGATAAGGTTGCTGC
  ATGATCTTAACTTTTCTGATGATGAGATTCACGGATTCACCGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG216118 representing NM_024195
  Red=Cloning site Green=Tags(s)
  
  MLNVPSQAFPAPGSQQRVSSQGRSKVPLKQGRSLMDWIRLTKSGKDLTGLKGGLIEVTEEELKKHNKKED
  CWICIRGFVYNVSPYMEYHPGGEDELMRAAGADGTDLFNEVHRWVNYESMLKECLVGRMAVKPAVPKDCH
  EGKRVLNGMLPKSQMSDTLPRDVTDTLPREDLSSPSYDWFQTESSVTIVVYTKQKNISLDSVIVDLQDDS
  LRAEAVIKDHSYLVHVGLSHEVQENFSVRVIENVGKIEIVLQKKESVSWQCLGDHLEKHDSFIPKKDTGL
  YYRRCQLISKEDVTHDTRLFCLMLPPSTHLQVPVGQHVYLKLSVTGAEIVKPYTPVSDSLLSDFKEPVLS
  PNKYICFLIKIYPAGLFTPELDRLQIGDFISVSGPEGDFKVSKLQEVEDLFLLAAGTGFTPMVTVLNYAL
  SHMSSLRKVKLMFFNKTEDDIIWRCQLEKLALREKRFDVEFVLSAPSPEWNGKQGHISRALLSEFLQRSS
  ENSRAFLCICGPTPFTDEGIRLLHDLNFSDDEIHGFTA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024195
• ORF Size: 1584 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024195.2, NP_077157.2
• RefSeq Size: 2653 bp
• RefSeq ORF: 1587 bp
• Locus ID: 266690
• UniProt ID: Q3TDX8
• Cytogenetics: 9 E3.1
• Gene Summary: NADH-cytochrome b5 reductase involved in endoplasmic reticulum stress response pathway. Plays a critical role in protecting pancreatic beta-cells against oxidant stress, possibly by protecting the cell from excess buildup of reactive oxygen species (ROS).[UniProtKB/Swiss-Prot Function]