Aunip (NM_001081099) Mouse Tagged ORF Clone

CAT#: MG214067

Clhc1 (tGFP-tagged) - Mouse RIKEN cDNA 2610002D18 gene (2610002D18Rik), (10ug)

Product Images

Circular map for MG214067

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Aunip
• Synonyms: 2610002D18Rik; Clhc1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG214067 representing NM_001081099
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCATAGAGGGCCCGAGGAGGAGGCGTGTGGTGTGTGGTTGGACGCGGCGGCGCTGAAGAGGCGGA
  AGATGCAGACACATTTAATCAAACTAGGCACCAAAATGCTGCCATTGCTTCCTGGAGAGAGGAAACCTAA
  TGATCCTTTTACCCAAAGAAGAGGCACTAGACAGACCAGCATTGCATCTTTTGTCACGCTACAATCAGGA
  ATGGCAAGTGGTGGTAACCAGAAGAACATTTTCTCTCTCAAAGAAAATCAGACCAACAAAGAATGCAAGA
  GAACCCAGCTAGACTGTTTGGATGATGGCTTGCTATCACCTTTGGTCACTTCAGCTCCTGCAGACATCCA
  GGAAGCTGGGCATCCTCGGTCTCCCCAGATTTCAGGCTGCCAGGGTTTGGAAATGTCATCTTTGACTATG
  ATGTCTTTGACCCAGCCTGATGTCCTGATGGGCACTGGAGAGAGTAAAGGCCCCCTGGATTCTTCCTTTT
  CCCAATACTTGGAACGTTCTTGTTTGCTGGACCAAAGAGAGGCCAAGAGGAAAGGGGAAGGGCTTCGTGA
  ATCTAAGACAGACTGTCCAGGGATGGGAAGCCACATCAGACCACCGGGGAGTAAATGCCATCAGCCCTTG
  GACAAAGCTGAAATGGGCAAGAGGGGGCCTACCAAGGAAAACAGGCAGGCCCCTGTGCATCTTCAGACCT
  ATAGATCTGGATCCTGCAGTAGGAAAAAAACACTATTGGTGACAGAAAGCCCTTGTCCTCTTTCCTTATT
  TCCCTGGGACAGTGAAAGGAGTGACAGGGACTCCTGGAGTCAGCTTTTCACTGAGGATTCACAAGGCCAG
  CAAGTCATTGCCCACAACACTAAAATGCCTTTCCGAGATGTGACCAATGCTAGGAATCAAGGCTCAGGGC
  AGTTTCCTGACAGCCCACAGGCTCAGGGCCAAGACGGGCCTGCTCAGTTACATCTGCAGTCATACCTGCT
  CTTTACCCAGGACTCTGAGGGTAATCGGGTTATCAGGCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG214067 representing NM_001081099
  Red=Cloning site Green=Tags(s)
  
  MRHRGPEEEACGVWLDAAALKRRKMQTHLIKLGTKMLPLLPGERKPNDPFTQRRGTRQTSIASFVTLQSG
  MASGGNQKNIFSLKENQTNKECKRTQLDCLDDGLLSPLVTSAPADIQEAGHPRSPQISGCQGLEMSSLTM
  MSLTQPDVLMGTGESKGPLDSSFSQYLERSCLLDQREAKRKGEGLRESKTDCPGMGSHIRPPGSKCHQPL
  DKAEMGKRGPTKENRQAPVHLQTYRSGSCSRKKTLLVTESPCPLSLFPWDSERSDRDSWSQLFTEDSQGQ
  QVIAHNTKMPFRDVTNARNQGSGQFPDSPQAQGQDGPAQLHLQSYLLFTQDSEGNRVIRH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081099
• ORF Size: 1020 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081099.1, NP_001074568.1
• RefSeq Size: 1270 bp
• RefSeq ORF: 1023 bp
• Locus ID: 69885
• UniProt ID: E9Q6Z5
• Cytogenetics: 4 D3
• Gene Summary: DNA-binding protein that accumulates at DNA double-strand breaks (DSBs) following DNA damage and promotes DNA resection and homologous recombination. Serves as a sensor of DNA damage: binds DNA with a strong preference for DNA substrates that mimic structures generated at stalled replication forks, and anchors RBBP8/CtIP to DSB sites to promote DNA end resection and ensuing homologous recombination repair. Inhibits non-homologous end joining (NHEJ). Required for the dynamic movement of AURKA at the centrosomes and spindle apparatus during the cell cycle.[UniProtKB/Swiss-Prot Function]