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Prr12 (NM_175022) Mouse Tagged ORF Clone
CAT#: MG213851
- TrueORF®
Prr12 (tGFP-tagged) - Mouse proline rich 12 (Prr12), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_175022" in other vectors (2)
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Symbol | Prr12 |
| Synonyms | 6720469B10; BC058674 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG213851 representing NM_175022, codon optimized.
Due to the complexity of NM_175022, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCGCAACTACCCATCAGCTGGATTCGGCGACCCTCTGGGCGCCGGGGCCGGATGGTCCTATGAAA GGAGTGCTAAGGCTTCCCTCGTATACGGCAGCAGCCGCACTTCACATCCCGAGACGGACATCCTGCACCG ACAGGCATATGCAGCTCCTCACCCCCTGCAGTCATATGCAACAAACCATCACCCGGCAGGGCTGTCAGGC TTGTTTGACACGGGACTGCACCACGCCGGCAGCGCAGGGCCCGATGCTAGCGTGATGAATCTTATTAGCG CCCTGGAAAGCCGCGGCCCCCAGCCTGGCCCCTCTGCCTCATCTCTCCTGTCCCAGTTCCGGTCCCCCTC ATGGCAGACTGCTATGCACACTCCAGGACCGACTGAGCTGTTTATTAGTGGGGCCTTGCCAGGCTCCTCT ACATTTCCAAGCAGCTCCGCTCTGAGCGCTTACCAACACCCTGCTTCCTTCGGCTCCCGACCCTTCCCTG TCCCCTCTTCCCTGTCACTGCAGGACCCGCCGTTCAGCCCTCCCGCCAACGGCCTTCTGTCCCCACACGA TGTTCTTCATCTGAAACCAAGCCAGGCTCCAACTGTGCCCAGCTCCCTGGGATTTGAAAGACTGGCCGGG GGGGGGGTCCTCGGACCAGCCGGCCTCGGGCCTGCCCAGACCCCCCCATATCGCCCAGGACCACCAGATC CGCCTCCGCCCCCCCGACACCTCCCAACACAGTTTAATCTGCTGGCCTCAAGCTCTGCTGCCACAGCTGC AGAGCCGAGCTCCCCTCAATTGTATAATTTCAGTGGCGCGGCCCCCGGCCCGCCTCCCGAAAGGGCCTTG CCAAGACAGGATACAGTTATTAAACATTACCAGCGCCCCGCTAGCGCACAACCGCCTCCGCCACCCCCAC CTGCACACTCCCTGCAGCACTATCTCTCATGCGGAGGCTCATATCCAAGCATGGGCCATAGGGCCAGCCT CGCCTGTAGTCCTCTGGGTGGCGGCGAGCCCTCACCGGGGGCGGGAGAGCCATCTAAGGGGGGACCCTCA GGAGCAACTGCAGGAGCCGCAGGCCGGGCCACTGGCCCGGAAACAGCCGGAGGCGGGGCCGCAGGCGGTG GTGGGGGTTACCGCCCAATCATCCAGTCTCCTGGGTACAAGACATCTAAAGGCGGATATGGGCCAGCAAC AGGAGGCGCCACACGCCCACCGCCCCCTAGGAGCACAGCCACACCCAAGTGCCAGTCACTGGGAGGTCCT GCTGCGGCCTACGCCGCAGGTAAGGCTTCCGGGGCGGGTGGGGCCGGAAGTCAGGCCTATTCCCCCGGTC AGCCACAGGGCCTCCTCGGACCCCAGGCGTATGGTCAAGGGTTCGGGGGAGGTCAGGCTCAGGACCTGTC AAAGGGGCCTTCTTATTCTGGCGGCCCCCCACAGCCCCCTAGCGGCCCTCCTCCACCTGGCTTGGCCACA TGTCAGTCTTATTCTCCTGACCAGCTCCAGGGACAGTTGTACGGCGTGCAATCTGAACCCTACCCAGGGC CTGCTGCTCACAGCCAGGGGCTGCCCACTGCGAGTCCTAGTCTCAGCTACAGCACTGGACATTCTCCAGC CCTGTCTGGCCACGGAGGTGGTTGGGGTCCAAGCTCCCTCGGGGGCGGGGGCGAAGCTAGTCCTTCCCAT ATTATCCGCCCTCTGCAGTCTCCCCCTGCAACCGGTCGGCCACCCGGTGTGGGGAGCCCCGGTGCCCCGG GAAAATATCTGAGCAGCGTCCTCGCCTCCGCACCATTCCTGGCTCCTCCAGGAGCGAGTTCTTACGCGGC CGGCGCGGGCGGATATAAAGGCAAAGGTGACGGCTCTGAACTCCTGGCGGGGCCCGGAGGCTCCGCCGCT GAGAGAACTGAGGACGAAGAGTTCCTGATCCAGCACCTGCTTCAAGCTCCCTCACCTCCAAGGACATCTG GAGCTGACGGCCTGGTTGGCGAGGACGGTCCAGCTGACGCAGCAAAAGGTCTTGGTGGGTCAGGCGGCGC AGGAGGCGCCCCCGGAACACCGTATGAACTGGCCAAAGAGGACCCGCAGCGATATCACCTGCAGAGCGTG ATCCGCACAAGTGCTAGTCTTGACGAAGGGGCAACCGCCGCACTCGAGCTGGGCCTGGGGCGGATGAAAG ACAAGAAAAAGGGTCCGGAAAGAGGAGGCGAAACACCAGAAGGCCTGGCCACTAGTGTTGTGCATTATGG CGCTGGCGCTAAGGAGCTTGGCGCGTTCTTGCAGAAGTCTCCGCCCCCGCCCCCTCCCTCCGCTCAAGCA ACACAGCCGGCCCCACATGGTCTGCTGTTGGAAGCAGGCGGGCCTGACCTTCCTATGGTTCTTCCACCCC CACCACCCCAGCTGCTGCCCAGTGTGCTCAGTCATGCACCCTCTCCGTCTCCGTCCGCACCTAAAGTTGG AGTGCACCTCCTGGAGCCTGCCACCAGAGACGGGGCTCCTCAGCCCCCACCTCCCCCGCCTCCCCCCATG CCCCTGCAGCTGGAGGCTCACCTGAGAGGGCACGGGTTGGAACCTACAGCACCTTCACCCCGACTCAGAC CAGAGGAAAGTCTGGAGCCTCCCGGCGCCATGCAAGAGCTGCTCGGCGCACTTGAACCTTTGCCTTCAGG CCCCGGAGATCCCGGGGTGGGGCCCCCTAACAGCGAAGGGAAGGACCCCGCTGGCGCTTACAGGTCACCT TCTCCGCAAGGCACCAAAGCGCCTAGGTTTGTGCCTCTGACCAGTATCTGTTTCCCAGACAGCCTGCTGC AGGACGAGGAGCGGAGCTTCTTTCCCACTATGGAAGAGATGTTCGGGGGTGGCGCAGCCGACGACTATGG TAAGGCAGGACAGACTGAAGATGATGGCGACCCAAAGACCGGCGCGGGCCCTCCACCAGGTCCCACGGCT TACGATCCCTATGGACCTTACTGTGGTGGCCGCGCTTCTGGAACAGGGCCTGAAACACCAGGGCTGGGCT TGGATCACAATAAGCCCCCCGAGCTCCCATCAACCGTCAACGCAGAGCCGCTCGGACTCATCCAGTCCGG TCCACATCAGTCTGCCCCTCCTCCTCCTCCGCCGCCACCACCTCCACCCCCTGTGAGCGAGCCCAAGGGT GGACTGACAAGCCCTATCTTCTGCTCCACAAAGCCCAAAAAACTTCTGAAAACCAGCAGCTTCCACCTGC TCCGCCGCCGCGACCCCCCTTTTCAGACACCTAAAAAGCTTTACGCCCAGGAGTACGAATTCGAAGCGGA TGAGGATAAAGCGGATGTACCTGCCGATATACGGCTGAATCCCCGCAGATTGCCTGACCTCGTGTCTAGC TGCAGATCCAGACCCGCCCTCTCTCCTCTGGGCGACATCGATTTCTGCCCCCCAAACCCTGGACCCGATG GACCAAGACGCAGGGGAAGAAAACCAACCAAAGCTAAGAGAGATGGTCCCCCACGACCTAGGGGCAGACC GCGCATCAGACCTCTGGAGGGACCTGCTATGGCCGGACCAGCTTCCATAACGACGGACGGAGCTAAGAAA CCACGGGGAAGGGGTAGGGGGAGAGGGAGGAAGGCCGAGGAGATGGGCGGAACCCGGCTTGAACCACTGA AGCCATTGAAAATCAAGCTGTCCGTTCCCAAAGCTGGAGAAGGACTCGGTGCACCCAGCAACGACGTGAT CTCTGGAGTCGATCACAATTCACTGGACTCCAATTTGACCCGGGAGAAGATCGAAGCAAAGATTAAAGAG GTGGAGGAGAAGCAGCCAGAAATGAAATCCGGATTTATGGCATCCTTCCTGGATTTCCTGAAGAGCGGAA AGAGGCACCCCCCCCTTTATCAGGCAGGCCTGACACCTCCACTGTCTCCACCCAAGTCAGTCCCAGCCTC TGTCCCGACTCGGGGGCTGCAGCCTCCTCCTCCTACAGTCCCTACGGTCCCACACCCAGCTCCATCTGGC CCTTTTGGGCTCGGGGGAGCACTCGAAGCAGCTGAGAGCGAGGGCCTCGGTCTGGGATGTCCCTCCCCCT GCAAACGGTTGGACGAGGAACTGAAGCGCAACTTGGAAACTCTCCCCTCCTTCTCTTCTGATGAAGAAGA CAGCGTGGCTAAGAACAGGGACCTTCAGGAGTCTATCTCCAGCGCCATCAGCGCCTTGGACGACCCCCCG CTTACGGGACCTAAGGATACATCAACTCCAGAGGAGCCCCCGCTTGACACCGGGCCTACAGCGTCAGGAC CTCCACCCCTGCCAAGCTTGCCATCTTCAAATAGCTCCGGGACCCCTGAGCCGCCATTGCTGGAGGAAAA GCCGCCTCCGACTCCTCCTCCAGCCCCGACTCCGCAACCAGCACCGCCCCCGCCCCCACCCCCTCCCCCA GTCCCAGCACTGCCGAGCCCTACACCCCTCGTCACCCCGGTCGCCTCTTCCCCCCCTCCACCACCTCCTC CTCCCCCCCCACCACCTGCCCTGCCCTCTCCGCCCCCACCCCCACCACCCGCTCCCACAACCGTCCCACC CGTGGCGCCACCAGAGGAACCCCCTGCCCCCTCCCCTGAAGACCCTGAGCCCCCTGATGCCCGGCCACTG CACCTGGCCAAGAAGCAGGAGACTGCTGCTGTTTGTGGCGAGACGGATGAGGAGGCGGGGGAAAGCGGCG GGGAGGGAATTTTTCGAGAGCGCGACGAGTTCGTAATCAGAGCTGAGGACATACCTTCCCTGAAACTCGC CCTGCAAACAGGCAGGGAACCCCCGCCTATTTGGCGGGTGCAGAAAGCTCTGCTCCAGAAATTCACCCCA GAGATTAAAGATGGTCAGCGCCAGTTCTGTGCCACCTCCAACTACCTCGGCTACTTTGGGGACGCGAAAA ATAGGTATCAACGCCTGTACGTGAAGTTTCTCGAGAATGTCAACAAGAAAGATTACGTTCGCGTCTGCGC CAGAAAACCCTGGCACCGACCACCACTGCCTGTGAGGAGATCTGGACAAACTAAGGGCCCAACCCCTGTC GGGGGCAACAGCGCACCGCCTTCTAAGGTCCAGGCCCCACCACCAAAGCCGGAGACGCCAGAAAAAATGA CCTCCGAGAAACCTCCAGAGCCCGCTCCCGAGCCCGCCGTGCCTGAGCCTCCTGCTCCCGAAAAACCCAG CCCGCCCAGGCCAGTAGAGAAAGAGAAGGAGAAGGAGAAAGAAAAGGAGAAGGAGAAGGAAAGAGTGACT AGGCCGCTTAGGTCTGAAAGGGCTACGAGCGGGAGACAGATGAGGACGGACCGATCTCTTGCTACCGGCC AATCTACCACCTCCCGGCTTCCTAAAGCACGCCCCTCTAAAGTTAAAGCCGAGCCCCCTCCGAAAAAGAG AAAGAAGTGGCTGAAAGAGGCTGTCGGCAATGCATCTGCAGGAGACGGCCCTGGCGGTTCTTCTTCTGAC TCCGAATCCTCCCCTGGCGCACCTTCTGAAGATGAACGCGCCGTGCCGGGACGCCTCCTCAAGACACGCG CAATGCGGGAAATGTACAGATCTTATGTAGAGATGCTGGTGAGCACTGCCCTGGACCCTGATATGATACA AGCTCTCGAAGACACTCATGACGAGCTGTACTTGCCCCCCATGAGGAAGATCGATGGGCTTCTGAATGAA CACAAGAAAAAGGTGTTGAAGCGATTGTCTCTGTCACCTGCACTGCAGGACGCCCTCCACACCTTTCCCC AGCTGCAAGTGGAACAGACCGGGGAGGGAAGTCCCGAGGAGGGTGCTGTCCGGCTGCGCCCAGCTGGTGA GCCATACAATCGCAAGACGCTGTCCAAGCTCAAAAGAAGTGTCGTGCGGGCTCAGGAATTCAAAGTCGAA TTGGAGAAATCAGGGTATTATACTCTCTATCATTCTCTGCATCATTACAAGTATCACACTTTCCTGCGGT GCCGGGATCAGACCCTGGCGATAGAAGGAGGCGCCGAGGATTTGGGACAGGAAGAGGTGGTGCAGCAGTG CATGAGGAATCAGCCCTGGCTCGAGCAGCTGTTTGACAGCTTCAGCGACTTGCTGGCACAGGCCCAAGCC CATAGCAGGTGCGGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG213851 representing NM_175022
Red=Cloning site Green=Tags(s) MDRNYPSAGFGDPLGAGAGWSYERSAKASLVYGSSRTSHPETDILHRQAYAAPHPLQSYATNHHPAGLSG LFDTGLHHAGSAGPDASVMNLISALESRGPQPGPSASSLLSQFRSPSWQTAMHTPGPTELFISGALPGSS TFPSSSALSAYQHPASFGSRPFPVPSSLSLQDPPFSPPANGLLSPHDVLHLKPSQAPTVPSSLGFERLAG GGVLGPAGLGPAQTPPYRPGPPDPPPPPRHLPTQFNLLASSSAATAAEPSSPQLYNFSGAAPGPPPERAL PRQDTVIKHYQRPASAQPPPPPPPAHSLQHYLSCGGSYPSMGHRASLACSPLGGGEPSPGAGEPSKGGPS GATAGAAGRATGPETAGGGAAGGGGGYRPIIQSPGYKTSKGGYGPATGGATRPPPPRSTATPKCQSLGGP AAAYAAGKASGAGGAGSQAYSPGQPQGLLGPQAYGQGFGGGQAQDLSKGPSYSGGPPQPPSGPPPPGLAT CQSYSPDQLQGQLYGVQSEPYPGPAAHSQGLPTASPSLSYSTGHSPALSGHGGGWGPSSLGGGGEASPSH IIRPLQSPPATGRPPGVGSPGAPGKYLSSVLASAPFLAPPGASSYAAGAGGYKGKGDGSELLAGPGGSAA ERTEDEEFLIQHLLQAPSPPRTSGADGLVGEDGPADAAKGLGGSGGAGGAPGTPYELAKEDPQRYHLQSV IRTSASLDEGATAALELGLGRMKDKKKGPERGGETPEGLATSVVHYGAGAKELGAFLQKSPPPPPPSAQA TQPAPHGLLLEAGGPDLPMVLPPPPPQLLPSVLSHAPSPSPSAPKVGVHLLEPATRDGAPQPPPPPPPPM PLQLEAHLRGHGLEPTAPSPRLRPEESLEPPGAMQELLGALEPLPSGPGDPGVGPPNSEGKDPAGAYRSP SPQGTKAPRFVPLTSICFPDSLLQDEERSFFPTMEEMFGGGAADDYGKAGQTEDDGDPKTGAGPPPGPTA YDPYGPYCGGRASGTGPETPGLGLDHNKPPELPSTVNAEPLGLIQSGPHQSAPPPPPPPPPPPPVSEPKG GLTSPIFCSTKPKKLLKTSSFHLLRRRDPPFQTPKKLYAQEYEFEADEDKADVPADIRLNPRRLPDLVSS CRSRPALSPLGDIDFCPPNPGPDGPRRRGRKPTKAKRDGPPRPRGRPRIRPLEGPAMAGPASITTDGAKK PRGRGRGRGRKAEEMGGTRLEPLKPLKIKLSVPKAGEGLGAPSNDVISGVDHNSLDSNLTREKIEAKIKE VEEKQPEMKSGFMASFLDFLKSGKRHPPLYQAGLTPPLSPPKSVPASVPTRGLQPPPPTVPTVPHPAPSG PFGLGGALEAAESEGLGLGCPSPCKRLDEELKRNLETLPSFSSDEEDSVAKNRDLQESISSAISALDDPP LTGPKDTSTPEEPPLDTGPTASGPPPLPSLPSSNSSGTPEPPLLEEKPPPTPPPAPTPQPAPPPPPPPPP VPALPSPTPLVTPVASSPPPPPPPPPPPPALPSPPPPPPPAPTTVPPVAPPEEPPAPSPEDPEPPDARPL HLAKKQETAAVCGETDEEAGESGGEGIFRERDEFVIRAEDIPSLKLALQTGREPPPIWRVQKALLQKFTP EIKDGQRQFCATSNYLGYFGDAKNRYQRLYVKFLENVNKKDYVRVCARKPWHRPPLPVRRSGQTKGPTPV GGNSAPPSKVQAPPPKPETPEKMTSEKPPEPAPEPAVPEPPAPEKPSPPRPVEKEKEKEKEKEKEKERVT RPLRSERATSGRQMRTDRSLATGQSTTSRLPKARPSKVKAEPPPKKRKKWLKEAVGNASAGDGPGGSSSD SESSPGAPSEDERAVPGRLLKTRAMREMYRSYVEMLVSTALDPDMIQALEDTHDELYLPPMRKIDGLLNE HKKKVLKRLSLSPALQDALHTFPQLQVEQTGEGSPEEGAVRLRPAGEPYNRKTLSKLKRSVVRAQEFKVE LEKSGYYTLYHSLHHYKYHTFLRCRDQTLAIEGGAEDLGQEEVVQQCMRNQPWLEQLFDSFSDLLAQAQA HSRCG TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175022 |
| ORF Size | 6105 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175022.2, NP_778187.2 |
| RefSeq Size | 6879 bp |
| RefSeq ORF | 6108 bp |
| Locus ID | 233210 |
| UniProt ID | E9PYL2 |
| Cytogenetics | 7 B3 |
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