Cfap70 (NM_001163639) Mouse Tagged ORF Clone

CAT#: MG213612

Ttc18 (tGFP-tagged) - Mouse tetratricopeptide repeat domain 18 (Ttc18) transcript variant 2, (10ug)

Product Images

Circular map for MG213612

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cfap70
• Synonyms: Ttc18
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG213612 representing NM_001163639
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCAGACGTCAAGCACAACAAAAACTGTGCACATTACTGTGACAAATGGATATGATTTGAAAGGCT
  TCAAAGGAGACACCCCAGTTACTTTTGTCCGTGCAGAATTCAACCAGACAGTTCTTGGAGACTCTTCAAA
  AGTCACTGTCTCTCCCGAAGGAACTGCAAAGTACAACTTTACTAGCAACATTGACCTCAGCCCAGATGGA
  GGCGGTGCCTTAGATGACCTCGCCCACAAGCCTTTATTCTTAACGGTGACTGAAGTTTTGCCAAAGGAAA
  AGAAACAGAAAGATGAAAAGACCTTAATTCTTGGCCAAGCTGTGGTGGATCTTCTTCCTTTACTAGAAGG
  AGAGGAGTCATTTGAAACAACGGTCCCATTACACCCTGTGCCTGGGTCACCCTTGGAAACTCCTTTGCCA
  GGCTCTAAGCAATGCAGTCTTGATGTGAAGGTATTTGTGGCCGAGCCCTTATTGACCCCAGCCCAGGTCT
  CAGCGAGCAATCTACTGAAGGTCACACTGGAGGCCGCCTACTCTGTGCCCGAGTCCTTCATACCAGTAGG
  GCCTCAGCAGAACTACATGGTCGGTCTGCAGGTCCCATCAGTCGGAGAGAAGGACTACACTATGATATTC
  AAGAATGGAAATCTGAAGCTTGGAGGGGAAAAAGAACCTGTTCCCCGGCCTAAAAAATGGCCCATTGCTA
  ACATTCTAGCTCCAGGAGCTAGTAACATTCCTGACGAATTCATTGTGGGGGGTCCCTATGAGGAAGAAGA
  AGGAGAACTCAACCACCCTGAGGACAGGGAATTTAGGAACCAAGCCGAATGCACGAAGAAGAGGATTGTC
  TGGGATTTGGAAAGCCGCTGCTTCCTCCATCCTTTTGCCGTTGCAAGTTTTCAGAAGCGAATTGCTGATT
  GCCGACTCTGGCCTGTGGAGATCACAAGAGTGCCTTTGGTCGTTATGCCAAAAGCCAAACCAGGAAAACT
  TGAGAAGATCGATGATGAAAACCAGCTTTCCTTTCATGGTGTGGCTTACATCAACATGGTCCCACTCCTG
  TATCCAGGAGTGAAGAAGATAAGAGGGGCTTTTCATGTGTACCCCTACCTGGATGGGACAGTCTTCGAAA
  AGACCAAGTGCTTGTTCAGCTTATTCAGGGACACTGGGCACCATTTGGTCCAAAATAATAAAGCAGGGGG
  ACTTAACTCTCCACTGTCCAAACCTCTCTCCAAGAATCTGAAAGAAGAGAAACCAGGGAAAGACAAGGAC
  ACAGAAGGACGGCCTCGGCTTGGAGAGCTGCAAGCGCCTAGTATAAAATCACAGAGCTCAGACACCCCTT
  TGGAAAGTGAAGCCCCCCTGAGCCACAACCTTGAAGGACAGCAATACATAGAAGCGGGAACATACATTGT
  CCTGGAGATCCAGCTGGAAAAAGCCCTGGTTCCAAAGCGGATGCCAGAGGAACTGGCCAGAAGGGTCAAG
  GAAATGATACCCCCGAGGCCACCTCTCACTCGCCGGACAGGGGGCGCTCAGAAGGCTGTGAGTGACTACC
  ACACGCAGATCAAGAGCATTTCTAGAGCCATACTCAATGAATACTACCGCATGTTTGGGAAGCAAGGGCC
  TAAACTGGAGAGTGACATAGACAACGAGACCATGGAGGAGCGGAAGTGTCAGCTCAACTATGAGCTCAAT
  TGCTCTGGAAAGTACTTTGCTTTCAAAGAACAGCTCAAGGCCGTCCCGGATGATGTCCCAAGCAGTACCT
  CAACCATTCAGACAAGCAGCGAGCAGCTCCGACTCTTTGCGTTTGAGGCAGAAGTCAATGAGAAGTTTGA
  AATAGCAGCAATGTATTATGAAGAGAGGTTGGTTCGGGAGCCCCAGAACCTGGAAAACTGGCTGGATTAC
  GGCGCTTTCTGTCTCCTCACTGAAGACAACATCAAAGCGCAGGAATGTTTCCGGAAAGCCCTTTCTCTGA
  ATGAGAGTCACGTTGACAGCTTGTTGCTGTGTGGTGTCCTGGCTATCTTGTTGGAGAACTATGAGCAAGC
  AGAAATCTTCTTTGAGGATGCTACATGCTTGGAACCCACCAATGTTATTGCCTGGACTTTACTCGGATTG
  TTCTATGAAATTCAGAATAATGATATTCGAATGGAAATGGCATTTCATGAGGCCTTCAAACAGCTTCAGG
  CGCGAACCCTCCAAACAAAGCTAAAAAGCACTGTGACCATAGAGAACATGGAGGAAGGAGTGAAAGTGGA
  GCCCAGCTTTGGCCCTTGGGGAGTCGTACAGGAGTCCACCACAGCCATCAAAACGGAAGGCCTATCAGGG
  ATGAGACCCCAGAGCTCACACCAGCTGAGTCCTCACACCAACATGGAACTGCATCCTCAGCCCCAAGGAC
  CAAACACTGCTTTGTCCAGTCTAGACGAGTTTTTGGAAGAATCGCCTAAGGCCCAGTCTGAGTCACAGGA
  GCCAATGGCCACTGGGCAGCCTCTGGAGCCTTCCCTGGTCCAGAGATCATCCAATGCACTGTTAAAGGAG
  CTGACGTCAAAGAAAGATATATCAAAATGCCAAGATTCATCAGCCTTTTCCCCTCCTACCCAGCACGTTA
  TTGCCCAGCCTCCTGTGACGATCTTCATGGAAACCATTCGCTTCTTGATGAAGGTCAACGCTGTACAGTT
  TGTGCACAGAGTGCTGGCACATGAGCTGCTGTGCCCCCAAGGAGGACCCAGTTGTGAGTATTATCTGGTT
  CTGGCCCAGACACATCTTCTCAAGAAGGACTTTGCCAAGACAGAGGAGTACCTGCAGCAAGCAGCACAGA
  TGGACTACCTGAACCCTAATGTCTGGGGTGTGAAGGGCCATCTCTACTTCCTGAGTGGAAACCATGCTGA
  GGCCAAAGAATGCTATGAGAGAACTATTAGTTTTGTAGTTGATGCTTCTGAGATGCACTTCATCTTCCTA
  CGCCTGGGACACATCTACTTGGAAGAGAAAGAGTATGAAAACGCAAAGAGAACGTACATGCATGCCTGTA
  AGAGATCACCTTCGTGCCTCACTTGGCTAGGACTAGGGATTGCCTGTTACCGGCTGGAGGAGCTCACGGA
  AGCTGAGGATGCTCTGTCTGAAGCCAACGCGTTGAATAACTGCAACGCCGAAGTGTGGGCCTATCTGGCT
  CTGGTCTGCCTGAAAGTTGGACGACAACTGGAAGCAGAGCAGGCTTATAAGTACACAATAAAGCTGAAAC
  TGAAAGACCAGGCTCTACTTGAAGAAATCCACACTGTACAGGAAATGGTTGGTTTTGGAAATCCATCTTT
  C
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG213612 representing NM_001163639
  Red=Cloning site Green=Tags(s)
  
  MDQTSSTTKTVHITVTNGYDLKGFKGDTPVTFVRAEFNQTVLGDSSKVTVSPEGTAKYNFTSNIDLSPDG
  GGALDDLAHKPLFLTVTEVLPKEKKQKDEKTLILGQAVVDLLPLLEGEESFETTVPLHPVPGSPLETPLP
  GSKQCSLDVKVFVAEPLLTPAQVSASNLLKVTLEAAYSVPESFIPVGPQQNYMVGLQVPSVGEKDYTMIF
  KNGNLKLGGEKEPVPRPKKWPIANILAPGASNIPDEFIVGGPYEEEEGELNHPEDREFRNQAECTKKRIV
  WDLESRCFLHPFAVASFQKRIADCRLWPVEITRVPLVVMPKAKPGKLEKIDDENQLSFHGVAYINMVPLL
  YPGVKKIRGAFHVYPYLDGTVFEKTKCLFSLFRDTGHHLVQNNKAGGLNSPLSKPLSKNLKEEKPGKDKD
  TEGRPRLGELQAPSIKSQSSDTPLESEAPLSHNLEGQQYIEAGTYIVLEIQLEKALVPKRMPEELARRVK
  EMIPPRPPLTRRTGGAQKAVSDYHTQIKSISRAILNEYYRMFGKQGPKLESDIDNETMEERKCQLNYELN
  CSGKYFAFKEQLKAVPDDVPSSTSTIQTSSEQLRLFAFEAEVNEKFEIAAMYYEERLVREPQNLENWLDY
  GAFCLLTEDNIKAQECFRKALSLNESHVDSLLLCGVLAILLENYEQAEIFFEDATCLEPTNVIAWTLLGL
  FYEIQNNDIRMEMAFHEAFKQLQARTLQTKLKSTVTIENMEEGVKVEPSFGPWGVVQESTTAIKTEGLSG
  MRPQSSHQLSPHTNMELHPQPQGPNTALSSLDEFLEESPKAQSESQEPMATGQPLEPSLVQRSSNALLKE
  LTSKKDISKCQDSSAFSPPTQHVIAQPPVTIFMETIRFLMKVNAVQFVHRVLAHELLCPQGGPSCEYYLV
  LAQTHLLKKDFAKTEEYLQQAAQMDYLNPNVWGVKGHLYFLSGNHAEAKECYERTISFVVDASEMHFIFL
  RLGHIYLEEKEYENAKRTYMHACKRSPSCLTWLGLGIACYRLEELTEAEDALSEANALNNCNAEVWAYLA
  LVCLKVGRQLEAEQAYKYTIKLKLKDQALLEEIHTVQEMVGFGNPSF
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001163639
• ORF Size: 3291 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001163639.1, NP_001157111.1
• RefSeq Size: 3551 bp
• RefSeq ORF: 3294 bp
• Locus ID: 76670
• UniProt ID: D3YVL2
• Cytogenetics: 14 A3
• Gene Summary: Axoneme-binding protein that plays a role in the regulation of ciliary motility and cilium length.[UniProtKB/Swiss-Prot Function]