Ift43 (NM_029601) Mouse Tagged ORF Clone

CAT#: MG212657

Ift43 (tGFP-tagged) - Mouse RIKEN cDNA 1700019E19 gene (1700019E19Rik), (10ug)

Product Images

Circular map for MG212657

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ift43
• Synonyms: 1700019E19Rik; R75064
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212657 representing NM_029601
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGATTTACTGGACCTGGGCGAGGAGAGACGTCGCAGCTCGGCCACCTCCGGGGCCAAGATGGGAC
  GCCGGGCTCATCAGGAGTCGACTCAGGCTGAGAATTACTTAAACAGCAAGAATTCTTCACTGACTCAGAC
  TGGAGAGGCTCCCCCACCGAAACCACCTCGAAGGCAGGGAGGCTGGGCGGATGACTCCATGAAGATGGCG
  AATCACCGCCTCCGACAGCAGAACCTGATGGGGTCAGACGACGACGATATTCCTGTGATCCCAGACCTGG
  AAGATGTGCAGGAGGAAGACTTTGTGCTACAGGTGGCGTCTCCTCCAAGTATCCAGGTAAACCGAGTGAT
  GACCTACCGTGACCTGGACAATGACCTCATGAAGTACTCGGCCTTTCAGACTTTGGATGGAGAAATTGAC
  CTGAAACTCCTCACCAAAGTGCTGGCCCCAGAGCATGAAGTGCGAGAGGATGATGTGGGCTGGGACTGGG
  ACCATCTGTACACCGAGGTGTCCTCAGAGCTCCTCACTGAGTGGGACCTGCTGCGGGCAGAGAAGGAGGA
  TCCTGTGGGACCATCTAGGTACATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212657 representing NM_029601
  Red=Cloning site Green=Tags(s)
  
  MDDLLDLGEERRRSSATSGAKMGRRAHQESTQAENYLNSKNSSLTQTGEAPPPKPPRRQGGWADDSMKMA
  NHRLRQQNLMGSDDDDIPVIPDLEDVQEEDFVLQVASPPSIQVNRVMTYRDLDNDLMKYSAFQTLDGEID
  LKLLTKVLAPEHEVREDDVGWDWDHLYTEVSSELLTEWDLLRAEKEDPVGPSRYI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029601
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029601.3, NP_083877.1
• RefSeq Size: 771 bp
• RefSeq ORF: 588 bp
• Locus ID: 76411
• UniProt ID: Q9DA69
• Cytogenetics: 12 D2
• Gene Summary: As a component of IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is involved in ciliogenesis. Involved in retrograde ciliary transport along microtubules from the ciliary tip to the base.[UniProtKB/Swiss-Prot Function]