Depdc5 (NM_001025426) Mouse Tagged ORF Clone
CAT#: MG212021
- TrueORF®
Depdc5 (tGFP-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 1
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001025426" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Depdc5 |
Synonyms | AV016528 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG212021 representing NM_001025426
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAACCACAAAGGTCTACAAACTTGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTAG TCGTGAACCCTAAAGTATTTCCTCACATCAAACTTGGAGACATTGTGGAGATTGCTCACCCCAATGATGA GTACAGTCCTTTGCTTTTGCAAGTCAAGTCGCTTAAGGAAGATTTACAGAAAGAAACTATCAGTGTGGAC CAGACTGTGACTCAAGTATTCCGGCTAAGACCTTATCAAGATGTCTATGTGAATGTTGTAGACCCCAAGG ATGTGACTCTTGACCTGGTGGAATTGACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACT AAAGAAAAGTTTGGTGAGCACCTGTGCCTATATCACTCAAAAAGTGGAATTTGCTGGCATCAGGGCACAG GCTGGTGAGCTGTGGGTCAAGAATGAGAAGGTCATGTGTGGTTACATTAGTGAAGAGACCAGGGTGGTGT TCCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTTGATATTTA TGGGGATCTGTACTTTGAGAAAGCTGTGAACGGTTTCCTCGCCGACCTGTTTACTAAATGGAAGGAGAAG AACTGTAGTCATGAAGTGACTGTGGTCCTGTTTTCCAGAACTTTCTATGATGCAAAATCTATTGATGAAT TTCCTGAAATAAACCGAGCTTCAATTCAAGAGGATCACAAAGGGAGATTCTATGAGGACTTTTACAAAGT GGTGGTGCAGAATGAGAGAAGGGAAGAGTGGACTTCACTCCTCGTGACCATTAAAAAACTCTTCATCCAG TATCCAGTGTTGGTGCGACTGGAACAGGCAGGGGGCTTTCCTCAAGGAGACAATTCTACCTCAGCACAAG GAAACTACCTAGAGGCCATCAACTTGTCATTCAATGTGTTTGACAAGCACTACATCAACCGAAACTTTGA CCGAACTGGGCAGATGTCTGTGGTGATCACGCCGGGGGTGGGTGTCTTTGAAGTGGACCGCCTACTCATG ATCTTGACCAAGCAGCGGATGATTGATAATGGAATTGGTGTGGACTTAGTGTGCATGGGAGAGCAGCCAC TACATGCTGTGCCATTATTCAAGCTACATAACCGGAGTGTTCCGAGGGATTCTCGACTGGGTGATGATTA TAATATTCCTCACTGGATAAACCATAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAACAGTTTCACT CCACGGATAAAACTAGCAGGAAAGAAGTCTGCCTCTGAGAAAACCAAAAATGGTCGTGATACATCTCTCG GGACTCCAAAGGAATCTGAAAACACCCTTCCCATCCAAGTAGATTATGATGCCTACGATGCTCAAGTGTT CAGGCTGCCTGGCCCATCCCGGGCTCAGCGCCTCGCCACCTGCAGGTCTGTGAGAGAACAGGAGAATCAC AGTCGCAAAAGTGCAAGCTCCTGTGATGTCTCATCCAGCCCTTCCCTGCCAAGCCGTGCACTGCCCACTG AGGAAGTGAGGAGCCAGGCTTCTGATGACAGCTCCCTGGGCAAGAGCACCAACATCCTGATGATCCCTAA CCCCCACCTGCACCAGTATGAAGTCAGCAGCTCACTGGGCTACACCAGCACCCGAGATGTCCTGGAGAAC ATGATAGAACCCCCACAGAGGGACTCCAGTGCACCAGGAAGGTTCCATGTGGGTAGTGCAGAGTCCATGC TCCATGTCCGACCTGGAGGATACACGCCTCAGAGAGCGCTGATTAACCCCTTCGCCCCCTCGAGGATGCC CATGAAGCTCACCTCCAACAGAAGGCGCTGGATGCACACTTTTCCTGTAGGCCCATCTGGAGAGGCCATC CAGATCCATCATCAGACCCGGCAAAACATGGCAGAGCTGCAGGGCAGCAGGCAGAGGGACCCTACCCACT CCTCTGCAGAGCTGTTGGAGTTGGCATACCATGAAGCTGCCGGGAGGCACAGCACTTCCCGCCAGCCTGG TGACAGCATGTCCTTGAACTTCAGTGGAACGGAAGAGCTTTCTGTCAGCCTGCTTAGCAACAGCAGTACA GGTGTGAATCCTAGGACCCAGAACAAGGACTCCCTAGAGGACAGTGTTTCTACCTCTCCAGACCCAATTC TGACACTGTCTGCTCCCCCTGTAGTGCCAGGCTTCTGTTGCACAGTTGGAGTGGATTGGAAGTCTCTTAC TACTCCTGCATGCCTTCCCCTCACCACTGACTACTTCCCGGACCGTCAGGGCCTGCAGAATGACTACACA GAGGGCTGCTATGATCTCCTCCCAGAAGCAGACATGGACAGGAGGGATGAGGAGGGTGTTCAAATGACAG CCCAGCAAGTGTTTGAAGAGTTCATCTGCCAGCGTCTCATGCAGGGTTACCAAATCATAGTACAGCCAAA GACCCAGAAACCCAACACCACAGTCCCACCCCCATTGAGCAGTAGCCCACTCTATAGCAGAGGCCTTGTG TCCCGAAATCGCCCTGAGGAGGAAGGCCAGTATTGGTTAAGTATGGGCAGAACTTTCCATAAGGTGACAC TCAAGGACAAGATGATCACAGTAACTCGTTACCTTCCCAAGTACCCTTATGAATCTGCCCAGATCCATTA CACCTACAGCCTCTGCCCTTCCCACTCTGACTCGGAGTTTGTCTCCTGTTGGGTGGATTTCTGCCACGAA CGGCTCGAGGAATACAAGTGGAATTACTTAGATCAGTATATTTGTTCTGCTGGCTCTGAAGACTTCAGCT TAATCGAGTCTCTGAAGTTCTGGAGAACCCGATTTTTACTACTGCCAGCCTGTGTTACTGCCACCAAGCG CATCACAGAAGGGGAGGTGCACTGTGACATCTATGGGGACAAACCCCGTGCAGATGAAGATGAGTGGCAG CTTCTAGATGGCTTTATTCGCTTTGTAGAGGGCTTAAATCGCATCCGCAGGCGCCACCGCTCAGACCGCA TGATTCGGAAGGGGACTGCCATGAAAGGCTTGCAGATGACCGGGCCCATCTCTGCACACTCTCTCGAGGC AGCAGGCCCTCCAGTCGGCAAGAAAGGAACCTCAGCTCTGTCTGCCCTGCTGGAGATGGAAGCTAGTCAG AAGAGCCTAGGAGAACAGCAGACGACTGTGCACGGAAAAAGCTCCACTCAGCCAGCTGAGAACAGCAGTG TTGCCATGACTCCCACCTATGTGGACAGCCCACGCAAGGACGGGGCCTTCTTTATGGAGTTTGTCCGCAG CCCACGCACAGCATCATCTGCCTTCTACCCTCAGGCATCTGTGGACCAAACAGCTCCTCTGGTGTTAGAC AGCACCAGTTTAGGGGTCAGCACAGGCCAGCCCATGGATAGAGGCAACAACCAGACCTTTGGGAACTCCC AGAACATAGAACAGGCCTTTCCCTCTGCAAACTCTGGTGACTACAGCTCTCAGCAGCATGTAGCAAGCTC TCTAACATCGTCTTCTACCCTGGTGGAGATTCTGGAAGCCATGAAGCATCCCTCGACAGGAGTCCAGCTG CTGTCTGAACAGAAGGGCCTTTCACCGTGCTGCTTCATCAGTGCTGAGGTCGTACACTGGTTGATGAACA ACGTGGAGGGGGTCCAGACGCAGGCGATGGGCATAGACATCATGCAGAAAATGCTGGAAGAGCAGCTCAT AACGCATGCATCTGGTGAAGCATGGCGGACCTTCATCTATGGCTTCTATTTCTACAAGATAGTAATGGAC AAAGAACCGGAAAGAGTGGCTATGCAGCAGCCCTCTGCCCCCTGGTACACAGCAGGAGCGGATGACTTTG CCAGCTTCCAACGCAAGTGGTTTGAGGTGGCCTTTGTGGCAGAAGAGCTTGTGCACTCTGAGATTCCTGC CTTCCTCCTGCCCTGGCTACCTAGCCGGCCAGCCTCTTATGCAAGCAGACACAGCTCCTTTAGCCGAAGT TTTGGAGGACGGAGCCAGGCAGCTGCGCTGTTAGCTGCTACTGTCCCAGAGCAGAGGACTGTGACCCTGG ATGTTGATGTGAACAACCGAACGGACCGGCTGGAATGGTGCAGCTGTTACTACCATGGAAACTTCTCTCT CAATGCCGCCTTTGAGATCAAGCTACACTGGATGGCAGTGACTGCCACAGTGCTGTTTGAGATGGTCCAA GGTTGGCATCGAAAAGCTACCTCCTGTGGCTTCTTGCTGGTCCCAGTTTTGGAGGGTCCTTTTGCTCTGC CCAGTTACCTGTATGGTGATCCCCTGAGGGCCCAGCTCTTCATCCCGCTCAACCTCAGCTGCTTGCTCAA GGAGGGCAGCGAGCACCTGTTCGATAGTTTGGATTTGTGCAAGATAAATATTCTGTCTCCGCTTTTAACT TCCCTGCTGAGAATAAGCCACAGTATATCCATGTCACAGGAACGGTGTTCCTTCAGCTGCCGTACTCCAA ACGCAAGTTTTCAGGGCAGCAGCGGCGGCGACGGAACTCTACCAGCTCCACCAACCAAAACATGTTCTGT GAGGAGCGGGTTGGCTACAACTGGGCCTACAACACCATGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212021 representing NM_001025426
Red=Cloning site Green=Tags(s) MRTTKVYKLVIHKKGFGGSDDELVVNPKVFPHIKLGDIVEIAHPNDEYSPLLLQVKSLKEDLQKETISVD QTVTQVFRLRPYQDVYVNVVDPKDVTLDLVELTFKDQYIGRGDMWRLKKSLVSTCAYITQKVEFAGIRAQ AGELWVKNEKVMCGYISEETRVVFRSTSAMVYIFIQMSCEMWDFDIYGDLYFEKAVNGFLADLFTKWKEK NCSHEVTVVLFSRTFYDAKSIDEFPEINRASIQEDHKGRFYEDFYKVVVQNERREEWTSLLVTIKKLFIQ YPVLVRLEQAGGFPQGDNSTSAQGNYLEAINLSFNVFDKHYINRNFDRTGQMSVVITPGVGVFEVDRLLM ILTKQRMIDNGIGVDLVCMGEQPLHAVPLFKLHNRSVPRDSRLGDDYNIPHWINHSFYTSKSQLFCNSFT PRIKLAGKKSASEKTKNGRDTSLGTPKESENTLPIQVDYDAYDAQVFRLPGPSRAQRLATCRSVREQENH SRKSASSCDVSSSPSLPSRALPTEEVRSQASDDSSLGKSTNILMIPNPHLHQYEVSSSLGYTSTRDVLEN MIEPPQRDSSAPGRFHVGSAESMLHVRPGGYTPQRALINPFAPSRMPMKLTSNRRRWMHTFPVGPSGEAI QIHHQTRQNMAELQGSRQRDPTHSSAELLELAYHEAAGRHSTSRQPGDSMSLNFSGTEELSVSLLSNSST GVNPRTQNKDSLEDSVSTSPDPILTLSAPPVVPGFCCTVGVDWKSLTTPACLPLTTDYFPDRQGLQNDYT EGCYDLLPEADMDRRDEEGVQMTAQQVFEEFICQRLMQGYQIIVQPKTQKPNTTVPPPLSSSPLYSRGLV SRNRPEEEGQYWLSMGRTFHKVTLKDKMITVTRYLPKYPYESAQIHYTYSLCPSHSDSEFVSCWVDFCHE RLEEYKWNYLDQYICSAGSEDFSLIESLKFWRTRFLLLPACVTATKRITEGEVHCDIYGDKPRADEDEWQ LLDGFIRFVEGLNRIRRRHRSDRMIRKGTAMKGLQMTGPISAHSLEAAGPPVGKKGTSALSALLEMEASQ KSLGEQQTTVHGKSSTQPAENSSVAMTPTYVDSPRKDGAFFMEFVRSPRTASSAFYPQASVDQTAPLVLD STSLGVSTGQPMDRGNNQTFGNSQNIEQAFPSANSGDYSSQQHVASSLTSSSTLVEILEAMKHPSTGVQL LSEQKGLSPCCFISAEVVHWLMNNVEGVQTQAMGIDIMQKMLEEQLITHASGEAWRTFIYGFYFYKIVMD KEPERVAMQQPSAPWYTAGADDFASFQRKWFEVAFVAEELVHSEIPAFLLPWLPSRPASYASRHSSFSRS FGGRSQAAALLAATVPEQRTVTLDVDVNNRTDRLEWCSCYYHGNFSLNAAFEIKLHWMAVTATVLFEMVQ GWHRKATSCGFLLVPVLEGPFALPSYLYGDPLRAQLFIPLNLSCLLKEGSEHLFDSLDLCKINILSPLLT SLLRISHSISMSQERCSFSCRTPNASFQGSSGGDGTLPAPPTKTCSVRSGLATTGPTTPC TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001025426 |
ORF Size | 4590 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001025426.2, NP_001020597.1 |
RefSeq Size | 8213 bp |
RefSeq ORF | 4593 bp |
Locus ID | 277854 |
Cytogenetics | 5 17.35 cM |
Gene Summary | As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC224652 | Depdc5 (untagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 1, (10ug) |
USD 1,440.00 |
|
MR212021 | Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 1 |
USD 1,439.00 |
|
MR212021L3 | Lenti ORF clone of Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 1 |
USD 1,739.00 |
|
MR212021L4 | Lenti ORF clone of Depdc5 (mGFP-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 1 |
USD 1,739.00 |
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