Scrib (BC060689) Mouse Tagged ORF Clone

CAT#: MG211217

Scrib (tGFP-tagged) - Mouse scribbled homolog (Drosophila) (cDNA clone MGC:66791 IMAGE:6417366)

Product Images

Circular map for MG211217

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Scrib
• Synonyms: Scrb1, mKIAA0147, CRIB, SCRIB1, vartul
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211217 representing BC060689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGAAGTGCATCCCGCTCTGGCGTTGTAACCGGCACGTGGAGTCGGTGGATAAGCGGCACTGCTCGC
  TGCAGGTTGTGCCAGAGGAGATTTACCGCTACAGTCGTAGCCTCGAGGAGCTGCTCCTCGACGCCAACCA
  GCTGCGCGAGCTACCTAAGCCCTTCTTCCGACTGTTGAACTTGAGAAAGTTGGGCCTCAGTGACAACGAG
  ATCCAGCGGCTGCCTCCTGAAGTGGCCAACTTCATGCAGCTGGTGGAGCTAGATGTGTCCCGGAACGATA
  TTCCTGAGATACCTGAGAGCATAAAGTTCTGTAAGGCTCTGGAGATTGCAGACTTCAGTGGGAACCCCCT
  GTCTAGACTTCCGGATGGCTTCACACAGCTACGCAGCCTGGCTCACCTGGCCCTGAATGACGTGTCCCTG
  CAGGCACTGCCTGGAGATGTGGGCAACCTGGCCAATCTGGTGACCCTGGAACTCCGGGAGAACCTGCTTA
  AATCTCTCCCTGCGTCCCTGTCTTTCCTGGTGAAGCTGGAACAGCTGGATCTGGGAGGCAACGACCTGGA
  AGTGCTGCCTGACACCCTGGGGGCTCTGCCTAACCTTCGGGAGCTATGGTTAGACCGAAACCAACTGTCA
  GCGCTGCCCCCGGAGCTAGGCAATCTGCGGCGGCTGGTGTGCCTGGATGTGTCAGAGAACAGGCTGGAGG
  AGCTGCCTGTGGAGTTGGGTGGGCTGGCACTGCTCACGGATCTGCTGCTTTCCCAGAACTTGCTTCAGCG
  GCTGCCAGAGGGCATCGGTCAGCTGAAGCAGCTGTCTATCCTGAAGGTGGACCAGAACCGGCTGTGTGAG
  GTCACTGAGGCCATAGGGGACTGTGAGAACCTCTCGGAGCTGATCCTCACGGAGAACCTGTTAACGGCCT
  TACCCCACTCGCTGGGCAAGCTGACCAAGCTGACTAACCTCAATGTGGACCGGAACCATCTTGAGGTGCT
  GCCTCCTGAAATCGGAGGCTGTGTGGCCCTTAGTGTCCTCTCTTTGAGAGACAATCGCCTGGCTGTCCTC
  CCTCCTGAGCTTGCCCATACCGCTGAGCTGCACGTGCTTGATGTGGCTGGGAACCGGCTGCGGAGTCTGC
  CGTTTGCGCTCACCCACCTCAACCTCAAGGCACTGTGGCTGGCCGAGAACCAGGCACAGCCCATGCTCCG
  CTTCCAGACTGAGGATGATGCCCAGACGGGTGAAAAGGTGCTCACCTGCTACCTGCTGCCCCAGCAGCCC
  CTGCCAAGTCTCGAAGACGCTGGACAGCAGAGCAGTCCCTCAGAGAGCTGTAGTGATGCCCCTCTGAGCC
  GTGTCAGTGTCATCCAGTTCGAGGACACCCTGGAAGGTGAAGAGGATGCTGAGGAAGCTGCGGCCGAGAA
  GCGGGGCCTACAGCGTAGGGCCACACCTCATCCCAGTGAGCTCAAGGTGATGAAGAGGGGCATTGAAGAG
  CGCCGGAATGAGGCCTTCGTCTGCAAGCCTGACCCCAGCCCACCCTCGCCTTCAGAGGAGGAGAAGAGGC
  TGAGTGCAGAGTCTGCCTTGAGTGGAGGCTCTGTCCCGTCAGCAAGCACAGCCTCGGAAGGTGAGCCTGA
  GATCCTGCCGGCTGAGGTACAAGGGCTAGGCCAGCATGAGGCCATGCCTGCACAGGAGGAGTATACAGAA
  GACGACTATAACGAGCCCACAGTGCACTTTGCAGAGGACACACTGATCCCTAGGGAAGACGGTGAAAGTG
  AAGAGGGACAGCCTGAGGCTGCCTGGCCGCTGCCTAGTGGCCGGCAGAGGCTCATCCGCAAAGACACGCC
  CCACTACAAGAAGCACTTCAAGATCTCCAAGCTCCCACAGCCTGAAGCTGTCGTGGCCCTGCTGCAAGGG
  GTGCAGACCGACAGGGAGGGCCCCACTGCAGGTTGGCACAATGGCCCACACACACCTTGGGCTCCTCGAG
  CCCATGAGGAGGAAGAGGAGGAGGAGGAGGAGAACAGGGATGAGGAGGAAGGCGAGGCCACCACGGAAGA
  AGATGACAAAGAAGAGGCTGTGGCTTCTGCTCCCTCTGTCAAGGGGGTGTCGTTTGACCAGGCCAATAAC
  CTGCTGATAGAGCCTGCTCGCATTGAGGAGGAAGAGTTAACACTCACGATCGTGCGGCAGACGGGGGGCC
  TGGGCATCAGTATCGCAGGGGGAAAAGGCTCTACCCCCTACAAAGGAGATGACGAGGGCATTTTCATCTC
  TCGAGTGTCTGAAGAGGGCCCTGCAGCCCGTGCTGGAGTCCGAGTTGGTGACAAACTTCTTGAGGTGAAT
  GGTGTAGCCTTGCAGGACGCAGAGCACCACGAGGCCGTGGAAGCACTTCGGGGGGCAGGTGCTGCTGTGC
  AGATGCGAGTGTGGAGGGAAAGAATGGTGGAGCCGGAGAACGCAGTCACTATTACCCCCTTACGCCCTGA
  AGATGACTATAGTCCCCGAGAGTGGCGGGGAGGTGGCCTGCGCCTTCCCCTGCTCCAGCCTGAGACTCCT
  GTATCCCTCCGTCAGCGTCATGCTGCCTGTCTCGTGCGCAGTGAAAAGGGGCTGGGCTTCAGCATTGCTG
  GTGGAAAGGGCTCCACACCTTACCGGGCTGGTGATGGGGGCATCTTTATATCCCGCATTGCAGAGGGAGG
  GGCTGCTCACCGGGCAGGCACTCTACAGGTTGGCGACCATCTGCGACCACCGGAGGGAAGACGACTGAGG
  CTCCCTGTTCCCCTGGCAGTCAGCAACCACCCTCCCCTGATGAACTGCCAGCCAATG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211217 representing BC060689
  Red=Cloning site Green=Tags(s)
  
  MLKCIPLWRCNRHVESVDKRHCSLQVVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNE
  IQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSL
  QALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLS
  ALPPELGNLRRLVCLDVSENRLEELPVELGGLALLTDLLLSQNLLQRLPEGIGQLKQLSILKVDQNRLCE
  VTEAIGDCENLSELILTENLLTALPHSLGKLTKLTNLNVDRNHLEVLPPEIGGCVALSVLSLRDNRLAVL
  PPELAHTAELHVLDVAGNRLRSLPFALTHLNLKALWLAENQAQPMLRFQTEDDAQTGEKVLTCYLLPQQP
  LPSLEDAGQQSSPSESCSDAPLSRVSVIQFEDTLEGEEDAEEAAAEKRGLQRRATPHPSELKVMKRGIEE
  RRNEAFVCKPDPSPPSPSEEEKRLSAESALSGGSVPSASTASEGEPEILPAEVQGLGQHEAMPAQEEYTE
  DDYNEPTVHFAEDTLIPREDGESEEGQPEAAWPLPSGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQG
  VQTDREGPTAGWHNGPHTPWAPRAHEEEEEEEEENRDEEEGEATTEEDDKEEAVASAPSVKGVSFDQANN
  LLIEPARIEEEELTLTIVRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVN
  GVALQDAEHHEAVEALRGAGAAVQMRVWRERMVEPENAVTITPLRPEDDYSPREWRGGGLRLPLLQPETP
  VSLRQRHAACLVRSEKGLGFSIAGGKGSTPYRAGDGGIFISRIAEGGAAHRAGTLQVGDHLRPPEGRRLR
  LPVPLAVSNHPPLMNCQPM
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060689
• ORF Size: 2789 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060689, AAH60689
• RefSeq Size: 4321 bp
• RefSeq ORF: 2789 bp
• Locus ID: 105782
• Cytogenetics: 15 35.29 cM
• Gene Summary: Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.[UniProtKB/Swiss-Prot Function]