Mgea5 (NM_023799) Mouse Tagged ORF Clone

CAT#: MG211167

Mgea5 (tGFP-tagged) - Mouse meningioma expressed antigen 5 (hyaluronidase) (Mgea5)

Product Images

Circular map for MG211167

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mgea5
• Synonyms: 2810009A20Rik; 4833427O07Rik; 5830447M11Rik; AA408215; Hy5; mKIAA0679; Ncoat; OGA
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211167 representing NM_023799
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGCAGAAGGAGAGCCAGGCGGCGCTGGAGGAGCGGGAGAGCGAGCGCAACGCCAACCCTGCCGCCG
  CCTCGGGGGCTTCGTTGGAGCAGTCGGTAGCTCCCGCCCCCGGAGAAGATAACCCATCGGGAGCCGGGGC
  TGCGGCCGTGGTCGGGGCGGCCGGAGGGGCCCGCAGGTTCCTGTGCGGTGTCGTGGAAGGGTTTTATGGA
  AGACCTTGGGTTATGGAGCAGAGAAAAGAGCTCTTTAGAAGGCTTCAGAAATGGGAATTAAACACATACT
  TGTATGCTCCAAAAGATGACTACAAACATAGGATGTTTTGGCGAGAGATGTATTCAGTGGAAGAAGCTGA
  GCAACTTATGACTCTGATCTCTGCTGCACGAGAATATGAAATAGAATTCATCTATGCTATTTCGCCAGGA
  CTAGATATCACTTTTTCTAACCCCAAGGAAGTATCAACGTTGAAACGCAAATTGGACCAGGTCTCTCAGT
  TTGGGTGCAGGTCGTTTGCTTTGCTTTTCGATGATATTGACCATAATATGTGTGCAGCAGACAAAGAGGT
  GTTCAGTTCTTTTGCACATGCACAAGTTTCCATCACCAATGAAATTTATCAGTACCTGGGAGAGCCAGAA
  ACCTTCCTCTTCTGCCCTACAGAATACTGTGGCACTTTCTGTTATCCAAATGTATCTCAGTCTCCTTACT
  TGAGGACTGTGGGTGAGAAGCTTCTACCTGGAATTGAGGTGCTTTGGACAGGTCCAAAAGTTGTTTCAAA
  AGAAATTCCAGTGGAATCTATTGAAGAAGTTTCTAAGATCATTAAGAGAGCTCCAGTGATCTGGGATAAC
  ATTCATGCTAATGATTATGATCAGAAGAGGCTGTTTCTGGGTCCCTACAAAGGAAGATCCACAGAGCTCA
  TCCCACGGTTAAAGGGAGTACTAACCAATCCAAATTGTGAATTTGAAGCTAACTACGTTGCCATACACAC
  CCTTGCCACTTGGTACAAGTCAAACATGAACGGAGTGAGGAAGGATGTGGTGATGACTGACAGTGAAGAC
  AGTACTGTATCAATACAGATAAAGTTAGAAAATGAAGGCAGTGACGAAGACATTGAGACTGATGTTCTCT
  ACAGCCCACAGATGGCTTTAAAGCTGGCTTTAACTGAGTGGCTGCAGGAGTTCGGTGTCCCTCATCAGTA
  CAGCAGTAGGCAAGTTGCACACAGTGGAGCTAAGACAAGTGTAGTTGATGGGACTCCCTTAGTTGCTGCA
  CCCTCTTTAAATGCCACAACTGTAGTTACAACAGTTTACCAGGAGCCCATTATGAGCCAGGGAGCAGCTT
  TGAGTGGTGAACCCTCAGTTCTGACCAAGGAAGAGGAAAAGAAACAGCCAGATGAGGAACCCATGGATAT
  GGTGGTCGAAAAGCAAGAAGAAGCAGAGCACAAGAATGACAACCAGATACTAACTGAGATTGTTGAAGCG
  AAGATGGCAGAGGAGTTGAGACCAATGGACACGGATAAGGAGAGCATGGCTGAGTCCAAATCCCCAGAGA
  TGTCCATGCAGGAAGACTGCATTCCTGACGTGGCTCCTATGCAGACAGATGAGCAAACACAGAAGGAGCA
  GTTTGTGCCAGGTCCAAATGAAAAGCCTCTGTACACCGCAGAGCCTGTGACTCTGGAGGACTTACAGTTA
  CTCGCTGACCTGTTCTACCTTCCTTACGAGCACGGGCCCAAAGGAGCGCAGATGCTACGGGAATTCCAGT
  GGCTTCGAGCAAACAGCAGTGTTGTCAGTGTCAACTGCAAAGGGAAAGACTCAGAAAAAATAGAAGAATG
  GCGGTCACGAGCAGCCAAGTTTGAAGAGATGTGTGCACTAGTGATGGGGATGTTCACCCGGCTTTCCAAC
  TGTGCCAACAGGACCATCCTTTATGACATGTACTCCTATGTGTGGGACATCAAGAGTATAATGTCTATGG
  TGAAATCTTTTGTGCAGTGGTTAGGGTGTCGTAGTCACTCTTCAGCACAGTTCTTAATTGGAGACCAGGA
  GCCCTGGGCCTTTAGAGGTGGTCTAGCAGGAGAGTTCCAGCGTTTGCTGCCAATTGATGGAGCAAATGAT
  CTCTTTTTTCAACCACCCCCACTGACTCCAACCTCCAAAGTTTATACTATCAGACCTTATTTCCCTAAAG
  ATGAGGCTTCCGTGTACAAGATTTGCAGAGAAATGTATGATGATGGAGTGGGTTTCCCTTTTCAAAGTCA
  ACCTGACCTTATTGGAGACAAGTTAGTAGGAGGACTGCTCTCCCTCAGCCTGGATTACTGCTTCGTCCTA
  GAAGATGAAGATGGCATTTGTGGTTATGCCTTAGGCACAGTAGATGTGACCCCCTTCATTAAAAAGTGTA
  AAATTTCCTGGATTCCCTTCATGCAAGAGAAGTATACCAAGCCAAATGGTGACAAAGAGCTCTCAGAGGC
  TGAGAAAATAATGTTGAGTTTCCATGAAGAGCAGGAGGTGTTGCCAGAAACTTTCCTTGCCAACTTCCCT
  TCTCTGATAAAAATGGATATTCATAAAAAAGTGACTGACCCGAGTGTTGCCAAAAGCATGATGGCTTGCC
  TCCTGTCTTCACTGAAGGCCAATGGCTCCCGGGGGGCTTTCTGTGAAGTAAGACCAGATGATAAAAGGAT
  TCTGGAATTTTACAGCAAGTTAGGCTGTTTTGAAATTGCAAAAATGGAAGGATTTCCAAAGGATGTGGTT
  ATACTTGGTCGAAGCCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211167 representing NM_023799
  Red=Cloning site Green=Tags(s)
  
  MVQKESQAALEERESERNANPAAASGASLEQSVAPAPGEDNPSGAGAAAVVGAAGGARRFLCGVVEGFYG
  RPWVMEQRKELFRRLQKWELNTYLYAPKDDYKHRMFWREMYSVEEAEQLMTLISAAREYEIEFIYAISPG
  LDITFSNPKEVSTLKRKLDQVSQFGCRSFALLFDDIDHNMCAADKEVFSSFAHAQVSITNEIYQYLGEPE
  TFLFCPTEYCGTFCYPNVSQSPYLRTVGEKLLPGIEVLWTGPKVVSKEIPVESIEEVSKIIKRAPVIWDN
  IHANDYDQKRLFLGPYKGRSTELIPRLKGVLTNPNCEFEANYVAIHTLATWYKSNMNGVRKDVVMTDSED
  STVSIQIKLENEGSDEDIETDVLYSPQMALKLALTEWLQEFGVPHQYSSRQVAHSGAKTSVVDGTPLVAA
  PSLNATTVVTTVYQEPIMSQGAALSGEPSVLTKEEEKKQPDEEPMDMVVEKQEEAEHKNDNQILTEIVEA
  KMAEELRPMDTDKESMAESKSPEMSMQEDCIPDVAPMQTDEQTQKEQFVPGPNEKPLYTAEPVTLEDLQL
  LADLFYLPYEHGPKGAQMLREFQWLRANSSVVSVNCKGKDSEKIEEWRSRAAKFEEMCALVMGMFTRLSN
  CANRTILYDMYSYVWDIKSIMSMVKSFVQWLGCRSHSSAQFLIGDQEPWAFRGGLAGEFQRLLPIDGAND
  LFFQPPPLTPTSKVYTIRPYFPKDEASVYKICREMYDDGVGFPFQSQPDLIGDKLVGGLLSLSLDYCFVL
  EDEDGICGYALGTVDVTPFIKKCKISWIPFMQEKYTKPNGDKELSEAEKIMLSFHEEQEVLPETFLANFP
  SLIKMDIHKKVTDPSVAKSMMACLLSSLKANGSRGAFCEVRPDDKRILEFYSKLGCFEIAKMEGFPKDVV
  ILGRSL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_023799
• ORF Size: 2748 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_023799.2
• RefSeq Size: 4797 bp
• RefSeq ORF: 2751 bp
• Locus ID: 76055
• UniProt ID: Q9EQQ9
• Cytogenetics: 19 38.75 cM
• Gene Summary: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:16517082). Does not bind acetyl-CoA and does not have histone acetyltransferase activity.[UniProtKB/Swiss-Prot Function]