Ercc4 (BC026792) Mouse Tagged ORF Clone

CAT#: MG211135

Ercc4 (tGFP-tagged) - Mouse excision repair cross-complementing rodent repair deficiency, complementation group 4 (cDNA clone MGC:30522

Product Images

Circular map for MG211135

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ercc4
• Synonyms: AI606920; Xpf
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211135 representing BC026792
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCGCTGTTGGAGTACGAGAGGCAGCAGGTGCTGGAACTGCTCGACAGCGACGGGCTGGTGGTGT
  GTGCCCGCGGACTCGGCACCGACCGGCTCCTCTACCATTTCCTGCGGCTTCACTGCCACCCGGCCTGCCT
  GGTGCTGGTGCTCAACACGCAGCCGGCCGAGGAGGAATATTTTATCAATCAGTTGAAGATAGAAGGAGTT
  GAACATCTCCCTCGACGAGTGACAAATGAAATCGCAAGTAACAGTCGCTATGAAGTCTACACGCAGGGTG
  GTATTATATTTGCAACAAGCCGAATACTCGTGGTTGATTTCTTGACAGGTAGAATACCTTCAGATTTAAT
  AACTGGCATCCTCGTGTACAGGGCACACAGAATCATTGAGTCCTGCCAGGAAGCCTTCATCCTGCGCCTC
  TTCCGCCAGAAAAACAAGCGCGGGTTCATCAAAGCTTTCACCGACAACGCTGTTGCCTTTGACACTGGCT
  TTTGTCACGTGGAAAGAGTGATGCGGAACCTTTTTGTGAGGAAGCTCTACCTGTGGCCAAGGTTCCATGT
  AGCAGTCAACTCATTCTTGGAACAACACAAGCCTGAAGTTGTAGAGATTCACGTGTCCATGACTCCCGCC
  ATGCTTGCCATTCAGACGGCCATACTAGACATCTTAAATGCATGTCTGAAAGAACTGAAATGTCACAACC
  CGTCACTTGAAGTAGAAGATTTGTCACTAGAAAACGCTCTTGGGAAGCCATTCGACAAGACAATCCGCCA
  TTACTTGGACCCTTTGTGGCATCAGCTTGGAGCCAAGACTAAATCCTTGGTGCAGGATTTAAAGATACTA
  AGAACCTTACTGCAGTATCTCTCTCAGTATGATTGTGTTACATTTCTGAATCTTCTGGAATCTCTGAGAG
  CAACAGAGAAGGTGTTTGGTCAGAATTCAGGTTGGCTTTTCCTGGATGCTAGCACCTCCATGTTTGTGAA
  TGCTCGTGCCCGAGTTTACCGAGTTCCAGATGTCAAACTGAACAAAAAGGCCAAAACATCAGAAAAGACG
  TCGAGTCCAGAAGTTCAAGAAACAAAAAAAGAACTGGTTCTAGAGAGCAATCCAAAGTGGGAGGCGCTGA
  CTGACGTCCTGAAAGAAATCGAAGCAGAAAATAAGGAGAGCGAAGCCCTTGGTGGCCCAGGCCGGGTGCT
  GATCTGTGCAAGCGATGACCGCACGTGCTGCCAGCTGAGAGACTACCTGTCCGCCGGAGCAGAAACCTTC
  CTGCTGCGACTTTATAGGAAAACTTTTGAGAAGGATGGCAAAGCTGAAGAAGTGTGGGTAAATGTTAGAA
  AGGGGGACGGCCCGAAGAGAACTACAAAATCTGACAAAAGACCAAAAGCCGCTCCAAACAAGGAGCGTGC
  TTCCGCCAAAAGGGGGGCCCCTCTCAAAAGGAAGAAGCAAGAGCTGACTCTGACGCAAGTTTTGGGGTCC
  GCTGAGGAGCCGCCGGAGGACAAGGCTTTGGAGGAAGACCTGTGCAGACAGACCAGTAGTAGCCCAGAAG
  GCTGCGGGGTAGAGATTAAGCGTGAATCATTTGATTTAAATGTGTCGTCTGATGCGGCCTATGGCATCCT
  GAAAGAGCCCCTGACCATCATCCATCCGCTTCTAGGGTGCAGCGACCCCTATGCCCTGACGCGGGTACTG
  CACGAGGTGGAGCCCAGATACGTGGTTCTGTATGATGCAGAGCTCACCTTTGTTCGCCAACTGGAGATCT
  ACAGGGCTAGCAGGCCTGGGAAACCTCTGAGGGTTTACTTTCTTATATACGGAGGCTCCACGGAAGAGCA
  GCGCTACCTGACTGCTCTGCGGAAAGAAAAGGAAGCCTTTGAAAAGCTCATAAGAGAAAAAGCTAGCATG
  GTTGTCCCCGAAGAACGGGAAGGCAGAGATGAGACCAACCTGGACCTAGCAAGAGGCTCAGCAGCCCTGG
  ATGCTCCCACGGACACTCGGAAAGCCGGTGGCCAGGAGCAGAATGGCACCCAGTCCAGCATCGTGGTGGA
  CATGCGTGAGTTTCGGAGCGAGCTCCCATCTCTGATCCATCGGCGGGGTATTGACATAGAGCCAGTGACT
  CTGGAGGTGGGTGATTACATCCTGACACCTGAGCTGTGTGTGGAGCGCAAAAGTGTGAGCGACCTCATTG
  GCTCGCTGCACAGTGGCCGCCTGTACAGCCAGTGCCTAGCCATGTCTCGCTACTACCGGAGGCCTGTGCT
  GCTCATTGAGTTTGACCCGAGCAAGCCCTTCTCCCTGGCGCCACGAGGTGCCTTCTTCCAGGAGATGAGC
  AGCAGCGATGTGAGCTCCAAGCTTACGCTCCTCACCTTGCACTTCCCGCGTTTGCGGCTGCTCTGGTGCC
  CCTCACCCCACGCCACAGCCGAGCTGTTTGAGGAGCTGAAGCAGAACAAGCCACAGCCTGATGCGGCCAC
  AGCCATGGCCATCACCGCAGACTCAGAGACACTGCCTGAGTCGGACAGATATAACCCCGGCCCCCAAGAC
  TTTGTGCTGAAGATGCCTGGGGTCAACGCCAAGAACTGTCGCTCCCTGATGAACCAAGTGAAGAACATCG
  CAGAGCTGGCCACCCTGTCCCTGGAACGACTCACAACCATCCTGGGGCACTCGGGAAATGCCAAGCAGCT
  GCACGACTTCCTGCACACTGCCTATGCAGACTTGGTGTCCAAAGGCAGAGTGAGAAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211135 representing BC026792
  Red=Cloning site Green=Tags(s)
  
  MAPLLEYERQQVLELLDSDGLVVCARGLGTDRLLYHFLRLHCHPACLVLVLNTQPAEEEYFINQLKIEGV
  EHLPRRVTNEIASNSRYEVYTQGGIIFATSRILVVDFLTGRIPSDLITGILVYRAHRIIESCQEAFILRL
  FRQKNKRGFIKAFTDNAVAFDTGFCHVERVMRNLFVRKLYLWPRFHVAVNSFLEQHKPEVVEIHVSMTPA
  MLAIQTAILDILNACLKELKCHNPSLEVEDLSLENALGKPFDKTIRHYLDPLWHQLGAKTKSLVQDLKIL
  RTLLQYLSQYDCVTFLNLLESLRATEKVFGQNSGWLFLDASTSMFVNARARVYRVPDVKLNKKAKTSEKT
  SSPEVQETKKELVLESNPKWEALTDVLKEIEAENKESEALGGPGRVLICASDDRTCCQLRDYLSAGAETF
  LLRLYRKTFEKDGKAEEVWVNVRKGDGPKRTTKSDKRPKAAPNKERASAKRGAPLKRKKQELTLTQVLGS
  AEEPPEDKALEEDLCRQTSSSPEGCGVEIKRESFDLNVSSDAAYGILKEPLTIIHPLLGCSDPYALTRVL
  HEVEPRYVVLYDAELTFVRQLEIYRASRPGKPLRVYFLIYGGSTEEQRYLTALRKEKEAFEKLIREKASM
  VVPEEREGRDETNLDLARGSAALDAPTDTRKAGGQEQNGTQSSIVVDMREFRSELPSLIHRRGIDIEPVT
  LEVGDYILTPELCVERKSVSDLIGSLHSGRLYSQCLAMSRYYRRPVLLIEFDPSKPFSLAPRGAFFQEMS
  SSDVSSKLTLLTLHFPRLRLLWCPSPHATAELFEELKQNKPQPDAATAMAITADSETLPESDRYNPGPQD
  FVLKMPGVNAKNCRSLMNQVKNIAELATLSLERLTTILGHSGNAKQLHDFLHTAYADLVSKGRVRK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC026792
• ORF Size: 2720 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026792, AAH26792
• RefSeq Size: 3235 bp
• RefSeq ORF: 2720 bp
• Locus ID: 50505
• Cytogenetics: 16 A1
• Gene Summary: Catalytic component of a structure-specific DNA repair endonuclease responsible for the 5-prime incision during DNA repair. Involved in homologous recombination that assists in removing interstrand cross-link (By similarity).[UniProtKB/Swiss-Prot Function]