Copg1 (NM_017477) Mouse Tagged ORF Clone

CAT#: MG211034

Copg1 (tGFP-tagged) - Mouse coatomer protein complex, subunit gamma (Copg), transcript variant 1

Product Images

Circular map for MG211034

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Copg1
• Synonyms: AU019265; BC056168; Copg; D6Ertd71e; D6Wsu16e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211034 representing NM_017477
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTGAAGAAATTCGACAAGAAGGACGAGGAGTCTGGTGGAGGCTCCAACCCCCTCCAGCACCTGGAGA
  AGAGTGCCGTACTCCAAGAGGCTCGGGTCTTTAACGAAACTCCCATCAATCCCCGGAAATGTGCTCACAT
  CCTCACCAAGATCCTTTATCTCATAAACCAGGGGGAGCACCTGGGGACCACGGAAGCAACTGAGGCTTTC
  TTTGCCATGACCAAGCTCTTCCAGTCCAATGATCCCACACTCCGCCGCATGTGCTATTTGACCATCAAGG
  AGATGTCCTGCATCGCTGAGGATGTCATCATTGTGACAAGCAGCCTGACAAAAGACATGACTGGGAAAGA
  GGATAATTACCGCGGTCCTGCTGTCCGTGCCCTCTGCCAGATCACTGACAGCACCATGCTGCAGGCTGTT
  GAACGCTACATGAAACAAGCAATTGTGGACAAGGTTCCCAGTGTCTCCAGCTCCGCCCTCGTGTCTTCCC
  TGCACCTGCTGAAATGCAGCTTCGATGTGGTCAAGCGCTGGGTGAACGAGGCCCAGGAGGCGGCGTCCAG
  TGACAACATCATGGTCCAGTACCATGCCCTAGGACTTCTGTACCATGTGCGGAAGAATGACCGACTGGCC
  GTGAGTAAGATGATCAGTAAGTTCACCCGGCACGGCCTCAAGTCCCCCTTTGCCTACTGCATGATGATCC
  GAGTGGCCAGCAAGCAACTAGAGGAAGAAGACGGCAGCCGTGACAGCCCGCTGTTTGACTTCATCGAGAG
  CTGCCTGCGTAACAAGCATGAGATGGTGGTGTATGAAGCTGCCTCAGCCATTGTCAACCTGCCAGGCTGC
  AGTGCCAAAGAGCTGGCCCCTGCTGTGTCAGTGCTCCAGCTTTTCTGTAGCTCACCCAAGGCTGCACTTC
  GCTATGCTGCTGTGCGCACCCTCAATAAGGTGGCTATGAAGCACCCTTCAGCGGTGACTGCCTGCAATCT
  AGATCTGGAAAACCTGGTTACAGACTCAAACAGGAGCATCGCCACATTGGCCATCACCACCCTGCTCAAG
  ACAGGGAGCGAGAGCAGCATTGACCGCCTTATGAAGCAGATTTCCTCCTTCATGTCGGAGATCTCAGATG
  AGTTCAAGGTGGTGGTTGTCCAGGCCATCAGTGCCCTGTGCCAGAAGTATCCTCGAAAGCATGCTGTGCT
  CATGAACTTCCTGTTCACCATGCTAAGGGAAGAGGGTGGCTTTGAGTACAAGCGTGCCATTGTGGACTGC
  ATCATCAGCATCATCGAGGAGAACTCAGAGAGCAAGGAGACAGGATTGTCACACCTGTGCGAGTTCATTG
  AGGACTGCGAGTTCACTGTGCTGGCCACCCGCATCCTGCACCTGTTGGGCCAGGAGGGGCCCAAGACCAA
  CAACCCTTCCAAGTACATTCGCTTTATCTACAACCGTGTGGTCTTGGAGCATGAAGAAGTTCGGGCAGGT
  GCAGTAAGTGCTCTGGCCAAGTTTGGAGCTCAGAACGAAGAGATGTTGCCTAGTATCTTGGTGTTGCTGA
  AGAGGTGTGTGATGGACGACGACAATGAAGTGAGAGACAGAGCCACCTTCTATCTGAACGTTCTGGAACA
  GAAGCAGAAGGCTCTCAACGCAGGTTACATCCTAAATGGTCTGACCGTGTCCATCCCTGGTCTGGAGAAA
  GCCCTGCAGCAGTACACACTCGAACCATCAGAGAAGCCATTTGACCTCAAGTCTGTGCCTCTGGCCACCA
  CGCCTATGGCAGAGCAGAGACCAGAAAGCACCGCCACCGCAGCAGTCAAACAGCCGGAGAAGGTAGCAGC
  CACACGGCAGGAGATTTTCCAAGAGCAGCTAGCAGCGGTGCCTGAGTTCCAGGGACTAGGGCCTCTCTTC
  AAGTCCTCTCCTGAGCCAGTGGCCCTCACAGAGTCCGAGACCGAGTATGTCATCCGTTGCACCAAACACA
  CCTTCTCTGATCACTTGGTGTTCCAGTTTGACTGCACAAACACCCTCAATGACCAGACTCTGGAGAATGT
  CACAGTGCAGATGGAGCCCACTGAGGCATACGAAGTGCTCTCGTATGTGCCTGCGCGGAGCTTGCCCTAC
  AACCAGCCTGGGACCTGCTACACACTAGTGGCTCTGCCCACTGAAGACCCCACAGCTGTGGCATGCACGT
  TCAGCTGTGTGATGAAGTTCACTGTTAAGGACTGTGATCCCAACACAGGAGAAATCGATGAAGAAGGCTA
  TGAGGATGAGTATGTGCTGGAGGATCTGGAAGTTACTGTGGCTGATCACATCCAAAAAGTCATGAAAGTG
  AACTTTGAGGCAGCCTGGGATGAGGTTGGGGATGAATTTGAGAAGGAGGAAACGTTCACCCTGTCTACTA
  TCAAGACACTCGAAGAGGCTGTGGGCAATATTGTGAAGTTCCTGGGAATGCCTCCTTGTGAGAGGTCAGA
  CAAAGTGCCAGAAAACAAGAACACCCACACGCTGCTGCTAGCTGGAGTATTCCGGGGTGGTCATGACATC
  CTTGTGCGTTCTCGGCTTCTGCTTTTGGACACAGTGACGATGCAGGTGACAGCCAGAAGCTCAGAGGAGC
  TGCCAGTAGATATCATCTTGGCATCCGTGGGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211034 representing NM_017477
  Red=Cloning site Green=Tags(s)
  
  MLKKFDKKDEESGGGSNPLQHLEKSAVLQEARVFNETPINPRKCAHILTKILYLINQGEHLGTTEATEAF
  FAMTKLFQSNDPTLRRMCYLTIKEMSCIAEDVIIVTSSLTKDMTGKEDNYRGPAVRALCQITDSTMLQAV
  ERYMKQAIVDKVPSVSSSALVSSLHLLKCSFDVVKRWVNEAQEAASSDNIMVQYHALGLLYHVRKNDRLA
  VSKMISKFTRHGLKSPFAYCMMIRVASKQLEEEDGSRDSPLFDFIESCLRNKHEMVVYEAASAIVNLPGC
  SAKELAPAVSVLQLFCSSPKAALRYAAVRTLNKVAMKHPSAVTACNLDLENLVTDSNRSIATLAITTLLK
  TGSESSIDRLMKQISSFMSEISDEFKVVVVQAISALCQKYPRKHAVLMNFLFTMLREEGGFEYKRAIVDC
  IISIIEENSESKETGLSHLCEFIEDCEFTVLATRILHLLGQEGPKTNNPSKYIRFIYNRVVLEHEEVRAG
  AVSALAKFGAQNEEMLPSILVLLKRCVMDDDNEVRDRATFYLNVLEQKQKALNAGYILNGLTVSIPGLEK
  ALQQYTLEPSEKPFDLKSVPLATTPMAEQRPESTATAAVKQPEKVAATRQEIFQEQLAAVPEFQGLGPLF
  KSSPEPVALTESETEYVIRCTKHTFSDHLVFQFDCTNTLNDQTLENVTVQMEPTEAYEVLSYVPARSLPY
  NQPGTCYTLVALPTEDPTAVACTFSCVMKFTVKDCDPNTGEIDEEGYEDEYVLEDLEVTVADHIQKVMKV
  NFEAAWDEVGDEFEKEETFTLSTIKTLEEAVGNIVKFLGMPPCERSDKVPENKNTHTLLLAGVFRGGHDI
  LVRSRLLLLDTVTMQVTARSSEELPVDIILASVG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017477
• ORF Size: 2622 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017477.2, NP_059505.1
• RefSeq Size: 4014 bp
• RefSeq ORF: 2625 bp
• Locus ID: 54161
• UniProt ID: Q9QZE5
• Cytogenetics: 6 39.13 cM
• Gene Summary: The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity). Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte triglyceride lipase (PNPLA2) with the lipid droplet surface to mediate lipolysis.[UniProtKB/Swiss-Prot Function]