Cdhr1 (NM_130878) Mouse Tagged ORF Clone

CAT#: MG210978

Cdhr1 (tGFP-tagged) - Mouse protocadherin 21 (Pcdh21)

Product Images

Circular map for MG210978

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdhr1
• Synonyms: mKIAA1775; Pcdh21; Prcad
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210978 representing NM_130878
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCGTGGCCCACGGGTCGCCCTGGTCCTGGGGCTGCTGCGCATTTACCTAGCCCAGGCCAACTTTG
  CCCCTCACTTCTTTGACAATGGAGTGGGCAGCACCAACGGGAACATGGCCCTGTTCAGCCTCCCAGAAGA
  CACTCCTGTAGGTTCTCATGTGTACACCCTAAATGGGACAGACCCTGAGGGAGACCCTATCTCCTACCAC
  ATCAGCTTTGACCCCAGCACTAGAAGTGTCTTTTCTGTTGATCCCAATTTTGGAAACATCACTCTGGTGG
  AAGAGCTGGACAGAGAAAGGGAAGATGAGATTGAAGCCATCATCAGCATTTCCGATGGCCTGAACCTGGT
  GGCAGAGAAAGTCGTGATACTGGTGACAGATGCCAATGATGAAGCACCCAGGTTCATCCAGGAGCCTTAC
  ATCATCCGGGTTCCAGAGAACATACCTGCTGGGAGCAGCATCTTTAAGGTCCAGGCAGAGGACAAGGACA
  CAGGCTCTGGAGGCAGTGTCACCTACTCCCTTCAGAACCTACACTCCTCCAAGTTCTCCATGGACCGTCA
  CAGCGGGGTGCTGAGACTCCAGGCTGGGGCCACACTGGACTATGAGAAGTCGAGGGCTCATTACATCACT
  GTGATTGCCAAGGATGGTGGGGGCAGGCTTCGAGGGGCTGACATGGTGTTCTCAGCCACCACCACCGTCA
  CCATCAATGTGGAAGATGTTCAGGACACGGCCCCTATCTTCGTGGGTACACCCTACTATGGTTACGTGTA
  TGAAGACACCCTTCCTGGCTCAGAGGTGCTGACGGTGGTTGCCATAGATGGAGACCGAGGCAAACCCAAC
  CACATCCTCTACAGGCTCTTGAATGAGAGTGACGGAATCTTTGAAATTAATGAGACATCTGGAGCCATCT
  CCGTCCTTCAGAGCCCTGCCCTGCTCCGAAGAGAGGTGTATGAACTTCATGTACAGGTGACAGAGGTCAA
  CTCTCCAGGAAGCCCAGCTGCTCAGGCCACAGTCCCAGTGACCATCAGGATCGTGGACCTCAACAACCAC
  CCTCCAACCTTCTATGGAGAGAGTGGGCCCCAGAACAAGTTTGAGCTCTCTATGTTTGAGCACCCACCCC
  AGGGGGAGATTCTCCGTGGACTCAAGATCACTGTCAACGACTCAGACCAGGGAGCCAATGCCAAATTCAA
  CTTACGGCTGGTGGGACCTGGAGGCATCTTCCGAGTGGTGCCACAGACCGTCCTGAATGAAGCCCAAGTG
  ACCATCATAGTGGAAAACTCAGCTGCCATTGACTTTGAAAAGTCTAAGTTGTTGACCTTCAAGCTCCTGG
  CCATTGAAGTGAACACCCCAGAGAAGTTCAGTTCCACAGCAGACATTGTGATCCAGCTTCTGGACACCAA
  TGATAATGTCCCCAAGTTCACCTCGCACTACTACATTGCCAGGATCCCAGAGAATGCTCCAGGAGGCTCC
  AACGTGGTGGCTGTCACAGCTGTGGATCCAGATACAGGACCATGGGGCAAAGTCCACTATTCCATCTATG
  GGACCGGGTCAGACCTGTTCTTAATTCACCCATCCACTGGGCTTATCTACACTCAGCCCTGGGCCAGCCT
  GGATGCTGAGGGTACTTCGAGGTACAACTTTTATGTGAAGGCAGAAGACATGGATGGAAGGTACAGCCTG
  GCTGAGGTGTTTGTCACTCTCTTGGATGTCAATGACCACTACCCCCAGTTTGTACAGAGCGTCCAGGAAA
  AGACAATGGTACTGGGGACCCCACTGAAAATTGAGGCAACAGACCAGGATGCAGAGGAGCCTAACAACTT
  GGTAGACTACTCCATTACCCGTGCAGAGCCAGTCAACGTGTTTGACATTGATGCACACACAGGGGAAATT
  AGGCTCAAGAATTCCATCCGCTCCTTGGAGGCCCTACACAACATCACACCCAGTGGGGACTACTCATGGT
  CCCTACAGGTGCAGGCCAAGGACCGAGGCTCCCCATCTTTCAGTACAACAGCCTTACTCAAGATCGACAT
  CACAGATACAGAGAGGCTATCCAGAGGTTCCATGGCTGCCTTCCTGATTCAGACCAAGGACAATCCCATG
  AAAGCCGTAGGTGTACTGGCTGGTGTCATGGCAATTGTTGTGGCCATAACTGTCCTCATCTCCACGGCTA
  CCTTCTGGCGTAACAAGAAGTCTAACAAGGTCCTGCCTGTGCGGCGTGTGCTCCGTAGAAGGCCCAGCCC
  TGCTCCCCACACAGTTCGAATAGAGTGGCTCAAATTCAGGAGGGCCAAGGCTGCTACCAAGTTTATACTC
  AAAGAGGATTCTCCCAATGAGAACTGCAATAACAGCAGAGTGGGAGTCATGGTGCCCCCAAGAGCTCCAG
  CTCTCCCACCACCCCCTAAAATGGCATCCAGTATGGTGGCCCAACAGACAGTGCCTACTGTCTCTGGATC
  CCTCACCCCACAGCCATCCCCACAGTTACCCACACCCAAGACCCTGGGAGGCCCCGTTCAGTCTTCTTTG
  GTGTCTGAGCTCAAGCAAAAGTTTGAGAAGAAGAGTTTAGACAACAAGGCCTATATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210978 representing NM_130878
  Red=Cloning site Green=Tags(s)
  
  MRRGPRVALVLGLLRIYLAQANFAPHFFDNGVGSTNGNMALFSLPEDTPVGSHVYTLNGTDPEGDPISYH
  ISFDPSTRSVFSVDPNFGNITLVEELDREREDEIEAIISISDGLNLVAEKVVILVTDANDEAPRFIQEPY
  IIRVPENIPAGSSIFKVQAEDKDTGSGGSVTYSLQNLHSSKFSMDRHSGVLRLQAGATLDYEKSRAHYIT
  VIAKDGGGRLRGADMVFSATTTVTINVEDVQDTAPIFVGTPYYGYVYEDTLPGSEVLTVVAIDGDRGKPN
  HILYRLLNESDGIFEINETSGAISVLQSPALLRREVYELHVQVTEVNSPGSPAAQATVPVTIRIVDLNNH
  PPTFYGESGPQNKFELSMFEHPPQGEILRGLKITVNDSDQGANAKFNLRLVGPGGIFRVVPQTVLNEAQV
  TIIVENSAAIDFEKSKLLTFKLLAIEVNTPEKFSSTADIVIQLLDTNDNVPKFTSHYYIARIPENAPGGS
  NVVAVTAVDPDTGPWGKVHYSIYGTGSDLFLIHPSTGLIYTQPWASLDAEGTSRYNFYVKAEDMDGRYSL
  AEVFVTLLDVNDHYPQFVQSVQEKTMVLGTPLKIEATDQDAEEPNNLVDYSITRAEPVNVFDIDAHTGEI
  RLKNSIRSLEALHNITPSGDYSWSLQVQAKDRGSPSFSTTALLKIDITDTERLSRGSMAAFLIQTKDNPM
  KAVGVLAGVMAIVVAITVLISTATFWRNKKSNKVLPVRRVLRRRPSPAPHTVRIEWLKFRRAKAATKFIL
  KEDSPNENCNNSRVGVMVPPRAPALPPPPKMASSMVAQQTVPTVSGSLTPQPSPQLPTPKTLGGPVQSSL
  VSELKQKFEKKSLDNKAYI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_130878
• ORF Size: 2577 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_130878.3
• RefSeq Size: 4364 bp
• RefSeq ORF: 2580 bp
• Locus ID: 170677
• UniProt ID: Q8VHP6
• Cytogenetics: 14 B
• Gene Summary: Potential calcium-dependent cell-adhesion protein. May be required for the structural integrity of the outer segment (OS) of photoreceptor cells.[UniProtKB/Swiss-Prot Function]