Rbm12b2 (NM_198957) Mouse Tagged ORF Clone

CAT#: MG210885

Rbm12b2 (tGFP-tagged) - Mouse RIKEN cDNA C430048L16 gene (C430048L16Rik)

Product Images

Circular map for MG210885

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rbm12b2
• Synonyms: AV299215; C430048L16Rik; Rbm12bb
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210885 representing NM_198957
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTAGTCATCCGTTTACTGGGGCTTCCTTTTATTGCGGGTCCTGTGGATATCCGTCACTTCTTCA
  AGGGATTGACTATTCCTGATGGAGGAGTGCATATAATTGGAGGGAAAGTTGGGGAGGCTTTTATAATTTT
  TGCAACAGATGAAGATGCAAGACGTGCTATAAGTCGCTCAGGAGGGTTTATCAAGGATTCATCTGTAGAG
  CTTTTTCTTAGTAGCAAGGTAGAAATGCAGAAGACCATAGAAATGAAAAGAACTGCTCGAGTAGGAAGAG
  GGCGACCAGGATCCGGGGCATCAGGGGTTGGCAACGTGTATCATTTTAGTGATGCTCTGAAGGAAGAAGA
  AAGTTATTCTGGATATGGCTCTTCGGTTAATCGAGATGCTGGGTTTCATACAAATGGTACAGGACTTGAC
  TTAAGGCCAAGAAAGACCAGGCCATTGAAGGCTGAGAATCCTTACTTATTTCTACGAGGTTTGCCTTACT
  TAGTAAATGACGATGATGTCCGTGTCTTTTTCTCTGGTTTGTGTGTAGATGGAGTAATTCTCTTAAAACA
  TCATGATGGCCGAAATAATGGTGATGCTATAGTAAAATTTGCTTCATGTGTTGATGCTTCAGGAGGTCTT
  AAATGCCATAGAAGTTTCATGGGTTCAAGATTTATAGAAGTCATGCAGGGTTCAGAACAACAGTGGATTG
  AATTTGGTGGTACTGCAACCGAGGGCGGTGACACTCCTCGTATGAGATCTGAAGAGCATTCTCCTTCAAG
  AAGGATTAATGGTAGACATTTTCGGAAACGGTCTCATTCAAAATCTCCTAGAGCACGTTCTCGCTCTCCT
  CTTGGATTTTATGTTCACTTAAAAAATCTGTCCTTAAATACTAACAAAAGAGATTTAAGGAATCTCTTTA
  GAGATACTGACCTGACTAATGACCAGATTAAGTTTGTGTATAAAGATGAAAGAAGAACACGCTATGCCTT
  TGTGATGTTCAAGAATCAAAAAGACTATAATACTGCCCTGGGTTTGCATAAGACTGTTTTACAATATCGT
  CCAGTCCTTATTGATCCAGTGTCTAGGAAGGAAATGGTGAGAATCATTGAATGCTATGAGAAGAAGAGAC
  CAGAGTCTTTAGAGAAGGAAAGGCCAGGACGTGTTTCACAAAAGTACTCTCAAGAAGGCTTCTCTGGCTC
  TGGTCAGAAGCTGTGCATATATATAAGAAATTTACCATTTGATGTTACAAAGGGTGAAGTGCAGAAGTTC
  TTTGCAGATTTTTCTCTTGTTGAGGATGACATTTATCTGCTTTGTGATGACAAGGGAGTTGGTTTGGGAG
  AAGCACTGGTGAGATTTAAATCAGAAGAACAAGCCATGAAAGCTGAACGTTTGAACCGACAAAGATTCTT
  GGGGATAGAGGTGTTGTTAAGACTTATATCTGAGGAACAAATGCAGGAGTTTGGTATAAAGTCTTCCTGG
  TTGTCTAATGAGAGGACACAAGCCTGTTCCAGGTCACATGATGGAGATGACTGTTCCTGTTTGTTTGACT
  TAAAAGATCCGTCATCCTGTTCATTTGGCCAGTCTGAAAGCCTTCGTTATCATCCAAAAGACTTAAGGAA
  AATGGGTCATTTCAAGCATCCCCAAGGGTATTTCCGGCAGTCTGACAGGTGCTCTCCTGAGGACTTCAGG
  CACTCCCCAGAAGACTATAGGCACCCCTGGGAGGAGCATACCAGCCACTCTAGGGAGGAAGACTGGAGAC
  TGCCTCTGGAGGATTGGCCACAGGAAGATGATTTTAGGCAGTGTCATGAGAAAGATCATAGGCAGCTAAG
  AAGCCCTTGGGAAGAAGACTTCAGACGGCCATCCCAGGAGCACTTCAGGAGATCCTATCAGGAGCACATT
  AGACGGCCACCCCAAGAACATTTCAGGCGTTCCCGAGAGGAAGATTTCAGGCATGTGGCTGATGAAGACT
  TTAGGCAAGCTTCTGATGAGGACTTCAGGATCTCCCAAGAAGATTTGAGATATCCCACTGATGAGGACTT
  TAGGCGGGTGTCTGTGGAAGACCTCAGGGAAGTTCCAGAGAAGGACCTTCGACTTCCTAAAAATTTTAGA
  TCTTCTGGAGAGGAGTTTTGGACCCCACCTGATTTCAGAGGTCAACATCCCTTTGTGAACTTTGATCATC
  TACAAGGTGGCAAATTTGATTTTGAAAAGTATAAATTGGAAAATTTTCATGACGGAAAATTTGTTCCTGA
  CCTAAAGTTTAATTGTGGTTCAGGTGGAATAATTCGTGTTATGATCTCGAATCTTCCATTCAAAGCCAAT
  GCTAATGAAATTCTAGACTTTTTTCATGGTTATAAAGTCATCCCTGATTCCGTTTCAATACAGTATAATG
  AGGAAGGATTGCCTTTAGGAGAAGCTATCGTTTCTATGACAAATTATAATGAAGCTCTATCTGCAGTTAA
  AGATCTAAGTGGTAGACCAGTTGGTCCCCGCAAAGTTAAGCTTAGTTTGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210885 representing NM_198957
  Red=Cloning site Green=Tags(s)
  
  MAVVIRLLGLPFIAGPVDIRHFFKGLTIPDGGVHIIGGKVGEAFIIFATDEDARRAISRSGGFIKDSSVE
  LFLSSKVEMQKTIEMKRTARVGRGRPGSGASGVGNVYHFSDALKEEESYSGYGSSVNRDAGFHTNGTGLD
  LRPRKTRPLKAENPYLFLRGLPYLVNDDDVRVFFSGLCVDGVILLKHHDGRNNGDAIVKFASCVDASGGL
  KCHRSFMGSRFIEVMQGSEQQWIEFGGTATEGGDTPRMRSEEHSPSRRINGRHFRKRSHSKSPRARSRSP
  LGFYVHLKNLSLNTNKRDLRNLFRDTDLTNDQIKFVYKDERRTRYAFVMFKNQKDYNTALGLHKTVLQYR
  PVLIDPVSRKEMVRIIECYEKKRPESLEKERPGRVSQKYSQEGFSGSGQKLCIYIRNLPFDVTKGEVQKF
  FADFSLVEDDIYLLCDDKGVGLGEALVRFKSEEQAMKAERLNRQRFLGIEVLLRLISEEQMQEFGIKSSW
  LSNERTQACSRSHDGDDCSCLFDLKDPSSCSFGQSESLRYHPKDLRKMGHFKHPQGYFRQSDRCSPEDFR
  HSPEDYRHPWEEHTSHSREEDWRLPLEDWPQEDDFRQCHEKDHRQLRSPWEEDFRRPSQEHFRRSYQEHI
  RRPPQEHFRRSREEDFRHVADEDFRQASDEDFRISQEDLRYPTDEDFRRVSVEDLREVPEKDLRLPKNFR
  SSGEEFWTPPDFRGQHPFVNFDHLQGGKFDFEKYKLENFHDGKFVPDLKFNCGSGGIIRVMISNLPFKAN
  ANEILDFFHGYKVIPDSVSIQYNEEGLPLGEAIVSMTNYNEALSAVKDLSGRPVGPRKVKLSLL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198957
• ORF Size: 2502 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198957.2, NP_945195.1
• RefSeq Size: 3457 bp
• RefSeq ORF: 2505 bp
• Locus ID: 77604
• UniProt ID: Q66JV4
• Cytogenetics: 4 A1