Adam8 (NM_007403) Mouse Tagged ORF Clone
CAT#: MG210842
- TrueORF®
Adam8 (tGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 8 (Adam8)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_007403" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Adam8 |
Synonyms | ADAM 8; CD156; CD156a; E430039A18Rik; MS; MS2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210842 representing NM_007403
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGGCCTCTGGCTGCTCAGCGTCTTATGGACACCAGTAGCCCCTGGACCTCCTTTGCCCCATGTGA AACAGTATGAAGTGGTTTGGCCTCGGCGCCTAGCTGCATCCCGCTCCCGCAGAGCCCTGCCCTCCCACTG GGGCCAGTACCCAGAGAGTCTGAGCTATGCTCTTGGGACCAGCGGGCACGTTTTCACCCTGCACCTTCGA AAGAACAGGGACCTGCTGGGCTCAAGCTACACAGAGACCTACTCAGCTGCCAATGGCTCTGAGGTGACAG AGCAACTGCAGGAGCAGGACCATTGCCTCTACCAAGGCCATGTGGAAGGGTACGAGGGCTCAGCTGCCAG TATTAGCACCTGTGCTGGCCTCAGGGGCTTTTTCCGAGTTGGGTCCACTGTCCACTTGATTGAGCCTCTG GATGCTGATGAAGAGGGGCAACATGCGATGTATCAGGCAAAGCATCTGCAACAGAAGGCTGGGACCTGTG GGGTCAAAGATACCAACCTGAATGACCTAGGGCCTCGGGCATTAGAAATATACAGGGCTCAGCCACGGAA CTGGCTGATACCCAGAGAAACCCGCTATGTGGAGTTGTATGTGGTTGCAGACAGCCAAGAGTTCCAGAAG TTGGGGAGCAGAGAGGCCGTGCGCCAGCGAGTGCTGGAGGTTGTAAACCACGTGGACAAGCTTTATCAGG AACTCAGTTTCCGAGTTGTCCTGGTGGGCCTGGAGATCTGGAACAAGGACAAATTCTACATCAGCCGCTA TGCCAACGTGACACTGGAGAACTTCTTGTCCTGGAGGGAACAGAACTTGCAAGGGCAGCACCCACATGAC AACGTGCAACTTATCACGGGGGTTGATTTCATTGGGAGCACTGTTGGACTGGCTAAGGTGTCTGCCCTGT GTTCCCGTCACTCCGGAGCTGTGAATCAGGACCACTCCAAGAACTCCATTGGTGTAGCCTCCACCATGGC CCATGAGCTGGGCCACAACCTGGGCATGAGCCATGATGAGGACATTCCAGGATGCTACTGTCCTGAACCA CGGGAGGGTGGTGGCTGCATCATGACCGAAAGCATCGGCTCCAAGTTCCCCAGGATATTCAGCAGGTGTA GCAAGATTGACCTAGAGTCATTCGTGACAAAACCCCAGACAGGCTGCCTGACCAATGTTCCAGATGTCAA CCGGTTCGTGGGTGGCCCTGTGTGTGGAAACCTGTTTGTGGAGCATGGAGAGCAGTGTGACTGTGGCACA CCTCAGGACTGTCAAAACCCCTGCTGCAATGCCACCACTTGCCAGCTGGTCAAGGGGGCAGAGTGTGCCA GTGGTACCTGTTGTCATGAATGCAAGGTGAAGCCAGCTGGAGAGGTGTGTCGTCTCAGTAAGGACAAATG TGACCTGGAGGAGTTCTGTGATGGCCGGAAGCCAACATGTCCCGAAGATGCCTTCCAACAGAATGGCACT CCCTGCCCAGGGGGCTACTGCTTTGATGGGAGCTGTCCCACCCTGGCACAGCAGTGCCGGGATCTGTGGG GGCCAGGTGCTCGGGTAGCAGCCGACTCCTGCTATACCTTTAGCATCCCTCCGGGCTGCAATGGGAGGAT GTACTCTGGCAGGATCAACCGGTGTGGAGCGCTGTACTGTGAGGGAGGCCAGAAGCCCCTTGAACGCTCC TTCTGCACTTTCTCCTCCAACCATGGAGTCTGCCATGCTCTTGGCACAGGCAGCAACATTGACACCTTTG AGCTGGTATTGCAGGGCACCAAGTGCGAGGAGGGAAAGGTTTGCATGGATGGAAGCTGCCAGGACCTCCG TGTATACAGATCTGAAAACTGCTCTGCTAAATGCAACAACCATGGGGTATGCAACCACAAGAGGGAGTGC CACTGTCACAAGGGCTGGGCACCACCCAACTGTGTACAGCGGCTGGCAGATGTATCAGATGAACAAGCAG CGTCTACGAGCCTCCCAGTCAGTGTGGTTGTGGTCTTGGTGATCCTGGTGGCTGCGATGGTCATCGTGGC AGGCATCGTCATCTACCGAAAGGCTCCGAGACAAATCCAGAGGAGGAGTGTGGCACCCAAGCCTATCTCG GGGCTCTCCAACCCCCTATTCTACACAAGGGACAGCAGCCTGCCAGCTAAGAACAGGCCTCCAGACCCTT CTGAGACAGTTTCTACCAACCAGCCCCCAAGACCCATAGTGAAACCAAAGAGGCCTCCCCCTGCACCTCC AGGTGCTGTGTCCAGTTCACCACTCCCAGTTCCTGTTTATGCCCCAAAGATACCAAATCAGTTTAGACCT GATCCTCCCACCAAGCCCCTCCCAGAGCTGAAACCCAAGCAGGTCAAGCCAACCTTTGCACCCCCGACAC CACCAGTCAAGCCCGGGACTGGAGGGACGGTGCCTGGAGCAACTCAGGGAGCTGGTGGGCCAAAGGTTGC TCTGAAGGTCCCCATCCAGAAGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210842 representing NM_007403
Red=Cloning site Green=Tags(s) MLGLWLLSVLWTPVAPGPPLPHVKQYEVVWPRRLAASRSRRALPSHWGQYPESLSYALGTSGHVFTLHLR KNRDLLGSSYTETYSAANGSEVTEQLQEQDHCLYQGHVEGYEGSAASISTCAGLRGFFRVGSTVHLIEPL DADEEGQHAMYQAKHLQQKAGTCGVKDTNLNDLGPRALEIYRAQPRNWLIPRETRYVELYVVADSQEFQK LGSREAVRQRVLEVVNHVDKLYQELSFRVVLVGLEIWNKDKFYISRYANVTLENFLSWREQNLQGQHPHD NVQLITGVDFIGSTVGLAKVSALCSRHSGAVNQDHSKNSIGVASTMAHELGHNLGMSHDEDIPGCYCPEP REGGGCIMTESIGSKFPRIFSRCSKIDLESFVTKPQTGCLTNVPDVNRFVGGPVCGNLFVEHGEQCDCGT PQDCQNPCCNATTCQLVKGAECASGTCCHECKVKPAGEVCRLSKDKCDLEEFCDGRKPTCPEDAFQQNGT PCPGGYCFDGSCPTLAQQCRDLWGPGARVAADSCYTFSIPPGCNGRMYSGRINRCGALYCEGGQKPLERS FCTFSSNHGVCHALGTGSNIDTFELVLQGTKCEEGKVCMDGSCQDLRVYRSENCSAKCNNHGVCNHKREC HCHKGWAPPNCVQRLADVSDEQAASTSLPVSVVVVLVILVAAMVIVAGIVIYRKAPRQIQRRSVAPKPIS GLSNPLFYTRDSSLPAKNRPPDPSETVSTNQPPRPIVKPKRPPPAPPGAVSSSPLPVPVYAPKIPNQFRP DPPTKPLPELKPKQVKPTFAPPTPPVKPGTGGTVPGATQGAGGPKVALKVPIQKR TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007403 |
ORF Size | 2475 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007403.2, NP_031429.1 |
RefSeq Size | 3034 bp |
RefSeq ORF | 2481 bp |
Locus ID | 11501 |
UniProt ID | Q05910 |
Cytogenetics | 7 F4 |
Gene Summary | This gene encodes a member of the Adam family of proteins that contain the disintegrin and metalloprotease domains. The encoded protein is localized to the cell surface, where it is involved in the remodeling of extracellular matrix and cell migration. Mice lacking the encoded protein display persistent inflammation upon treatment with allergens. Alternative splicing of this gene results in multiple variants. [provided by RefSeq, Mar 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203037 | Adam8 (untagged) - Mouse a disintegrin and metallopeptidase domain 8 (Adam8), (10ug) |
USD 756.00 |
|
MR210842 | Adam8 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 8 (Adam8) |
USD 756.00 |
|
MR210842L3 | Lenti ORF clone of Adam8 (Myc-DDK-tagged) - Mouse a disintegrin and metallopeptidase domain 8 (Adam8) |
USD 1,056.00 |
|
MR210842L4 | Lenti ORF clone of Adam8 (mGFP-tagged) - Mouse a disintegrin and metallopeptidase domain 8 (Adam8) |
USD 1,056.00 |
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