C2cd5 (NM_029081) Mouse Tagged ORF Clone

CAT#: MG210801

C2cd5 (tGFP-tagged) - Mouse RIKEN cDNA 5730419I09 gene (5730419I09Rik)

Product Images

Circular map for MG210801

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: C2cd5
• Synonyms: 5730419I09Rik; C030008B15Rik; CDP138
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210801 representing NM_029081
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGTGAATTACAGAGAAAGATTGGCTTGAAAGTCCTCGAGATGAGAGGGAATGCAGTTGTTGGTT
  ACTTGCAGTGCTTTGATCTGGAGGGCGAGTCTGGGTTAGTGGTCCGAGCCATAGGAACGGCGTGCACTCT
  GGATAAACTGAGCAGCCCAGCAGCGTTCCTTCCTGCATGCAGCTCCCCATCCAGAGAACTGAAGGAGATT
  CCCTTCAATGAGGATCCCAACCCCAATACTCACTCATCAGGACCCTCAACCCCTCTGAAAAACCAAACTT
  ATTCCTTTTCACCTTCCAAGTCCTACAGTCGGCAGTCCTCTTCTTCTGACACAGATTTGAGTTTGACACC
  CAAGACTGGAATGGGCAGTGGGAGTGCTGGAAAAGAAGGGGGACCCTTTAAAGCCCTTTTACGGCAACAG
  ACGCAGTCGGCGTTGGAACAGCGGGAGTTCCCGTTCCTCACCCTGACGGCCTTCCCCCCTGGACTTCTTG
  TGCACGTCGGTGGCGTTGTCAGTGCACGCTCTGTGAAGCTTTTAGATCGGATCCACAACCCTGATGAACC
  AGAAACTCGAGATGCATGGTGGGCAGAAATCAGACAAGAGATAAAGTCACATGCTAAAGCCTTAGGCTGC
  CATGCTGTAGTGGGCTACAGCGAGTCAACCAGCATCTGTGAAGAAGTCTGTATTTTATCAGCGTCTGGCA
  CGGCAGCTGTGCTGAACCCTCGGTTTCTGCAGGAAGGCACTGTGGAGGGCTGTTTGGAACAAAGGATTGA
  AGAGAATTTACCGGTGGGCTGCGGATTCTGCCATATACCATATGATGAACTGAACATGCCGTTTCCCGCT
  CATCTCACATATTGCTATAACTGCAGGAAGCAAAAAGTCCCTGATGTTCTCTTCACAACTATCGATCTGC
  CCACAGACGCAGTGGTTGTTGGTAAAGGTTGTCTTATTCAGGCAAGGTTATGTCGATTAAAAAAGAAAGC
  ACAGGCAGAAGCCAATGCCACAGCTATCAGTAATCTCTTGCCGTTTATGGAATATGAAGTACACACTCAA
  CTGATGAACAAGCTCAAACTCAAAGGAATGAATGCTTTGTTTGGACTGAGAATTCAGATCACAGTGGGCG
  AGACCATGCTGATGGGCTTAGCGTCGGCCACAGGAGTCTACTTAGCAGCCTTACCAACACCCGGTGGAAT
  CCAGATTGCTGGGAAGACCCCTAATGATGGCTCCTATGAACAGCATATCTCCCACATGCAGAAGAGAATA
  AACGACACCATTGCAAAAAACAAAGAACTGTATGAGATCAGCCCCCCGGAGGTGTCTGAGGAGATGATAG
  GATCCCCCATCCCAGAACCCAGACAGCGCTCCAGACTTCTAAGATCACAGTCGGAAAGTTCTGATGAAGT
  TACAGAGTTAGATCTTTCACACGGGAAAAAGGATGCTTTTGTTTTAGAGATCGATGACACCGATGCCATG
  GAAGACGTGCACTCCCTCCTTACTGACGCCCCACCCCCTTCAGGTTTTTATAGTTGTAATACAGAAATTA
  TGCCTGGCATAAATAACTGGACATCAGAAATACAGATGTTCACGTCAGTAAGAGTGGTCAGACTAAGTAG
  CCTCAACTTGACTAACCAAGCTCTCAATAAGAACTTCAACGGCCTATGTGAGAACTTGCTCAAGAGCCTG
  TATTTCAAACTTCGGTCCATGACCCCCTGCTGCCTCTGCCATGTGAACTTTACAGTGTCTCTGCCTGAAG
  ATGAGTTAATCCAGGTCACAGTCACAGCAGTTGCAATAACTTTTGATAAAAACCAGGCCTTACAGACCAC
  AAAACCCCACGTTGAAAAGTCACTACAAAGAGCCTCCACAGATAACGAAGAACTGCTGCAGTTCCCACTG
  GAGCTCTGCTCAGACTCACTGCCTCCTCATCCTTTCCCAGCAGCTAAAGAACACCTGGAGAGTGCGAACT
  CTAACTCAGGTATACCAGCTGCACAGAGAGCTGTGACTGTTGAAAAAGCAAGTGCCATGGGTGATGGAAA
  TTTCCGGAATCGCTCTGCTCCACCTTGCGCAAGTCCCACAGTTGGGGTTGTTAAGATGACGCCGCTTTCT
  TTCATCCCTGGAGCAAAGATAACCAAGTACCTCGGCATCATTAACATGTTTTTCATTCGGGAAACCACTT
  CTCTCCGCGAGGAAGGCGGAGTCAGTGGTTTTCTCCACGCTTTCATTGCTGAAGTGTTTGCCATGGTCCG
  AGCTCACGTGGCAGCGCTGGGCGGGAATGCTGTTGTGTCCTACATCATGAAGCAGTGTGTCTTCATGGAG
  AACCCCAGTAAGAACCAGGCACAGTGTCTCATAAATGTCAGTGGGGACGCGGTGGTGTTTGTTCGTGACT
  CCGACTTAGAGGTCATGTCATCTCAGCAGCCTGCTGCCAACTGCCAGCCCTCATGTACCGGAGAAGTTAC
  AACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210801 representing NM_029081
  Red=Cloning site Green=Tags(s)
  
  MSGELQRKIGLKVLEMRGNAVVGYLQCFDLEGESGLVVRAIGTACTLDKLSSPAAFLPACSSPSRELKEI
  PFNEDPNPNTHSSGPSTPLKNQTYSFSPSKSYSRQSSSSDTDLSLTPKTGMGSGSAGKEGGPFKALLRQQ
  TQSALEQREFPFLTLTAFPPGLLVHVGGVVSARSVKLLDRIHNPDEPETRDAWWAEIRQEIKSHAKALGC
  HAVVGYSESTSICEEVCILSASGTAAVLNPRFLQEGTVEGCLEQRIEENLPVGCGFCHIPYDELNMPFPA
  HLTYCYNCRKQKVPDVLFTTIDLPTDAVVVGKGCLIQARLCRLKKKAQAEANATAISNLLPFMEYEVHTQ
  LMNKLKLKGMNALFGLRIQITVGETMLMGLASATGVYLAALPTPGGIQIAGKTPNDGSYEQHISHMQKRI
  NDTIAKNKELYEISPPEVSEEMIGSPIPEPRQRSRLLRSQSESSDEVTELDLSHGKKDAFVLEIDDTDAM
  EDVHSLLTDAPPPSGFYSCNTEIMPGINNWTSEIQMFTSVRVVRLSSLNLTNQALNKNFNGLCENLLKSL
  YFKLRSMTPCCLCHVNFTVSLPEDELIQVTVTAVAITFDKNQALQTTKPHVEKSLQRASTDNEELLQFPL
  ELCSDSLPPHPFPAAKEHLESANSNSGIPAAQRAVTVEKASAMGDGNFRNRSAPPCASPTVGVVKMTPLS
  FIPGAKITKYLGIINMFFIRETTSLREEGGVSGFLHAFIAEVFAMVRAHVAALGGNAVVSYIMKQCVFME
  NPSKNQAQCLINVSGDAVVFVRDSDLEVMSSQQPAANCQPSCTGEVTT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029081
• ORF Size: 2454 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029081.1, NP_083357.1
• RefSeq Size: 4202 bp
• RefSeq ORF: 3051 bp
• Locus ID: 74741
• UniProt ID: Q7TPS5
• Cytogenetics: 6 G3
• Gene Summary: Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM.[UniProtKB/Swiss-Prot Function]