Hnrnpu (NM_016805) Mouse Tagged ORF Clone

CAT#: MG210713

Hnrnpu (tGFP-tagged) - Mouse heterogeneous nuclear ribonucleoprotein U (Hnrpu)

Product Images

Circular map for MG210713

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hnrnpu
• Synonyms: AA408410; AI256620; AL024194; AL024437; AW557595; C86794; hnRNP U; Hnrpu; SAFA; Sp120
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210713 representing NM_016805
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTTCTTCGCCTGTTAATGTCAAGAAGCTGAAGGTGTCGGAGCTGAAGGAGGAGCTCAAGAAGCGGC
  GCCTCTCCGACAAGGGCCTCAAGGCCGATCTCATGGATCGACTCCAGGCCGCGCTGGACAACGAGGCAGG
  AGGCCGCCCCGCCATGGAGCCCGGGAACGGCAGTCTCGACCTAGGTGGCGATGCGGCCGGGCGCTCGGGA
  GCGGGCCTAGAGCAGGAGGCCGCGGCTGGCGCCGAAGACGACGAGGAGGAGGAGGGCATCGCCGCTCTGG
  ACGGCGACCAGATGGAGCTGGGCGAGGAGAACGGGGCGGCGGGGGCGGCTGACGCCGGCGCGATGGAGGA
  AGAGGAGGCAGCGTCGGAAGACGAGAACGGCGACGACCAGGGCTTCCAGGAGGGGGAAGACGAGCTCGGC
  GACGAGGAGGAGGGCGCGGGCGACGAGAACGGTCACGGGGAGCAGCAGTCCCAACCGCCGGCAGCGGCGG
  CGCAGCAGCAGCCTTCCCAGCAGCGTGGTGCCGGCAAGGAGGCCGCGGGCAAGAGCAGCGGCCCCACCTC
  GCTCTTCGCGGTGACGGTGGCGCCGCCAGGGGCGAGGCAGGGCCAACAGCAGGCGGGAGGAGACGGCAAA
  ACAGAACAGAAAGGTGGAGATAAAAAGAGAGGCGTTAAAAGACCGCGAGAAGATCATGGCCGAGGGTATT
  TTGAGTACATCGAAGAAAACAAGTACAGCAGAGCCAAGTCTCCTCAGCCACCTGTTGAAGAAGAAGACGA
  ACACTTCGATGACACAGTGGTTTGTCTTGATACTTATAATTGTGATCTGCATTTTAAAATCTCGAGAGAC
  CGTCTGAGTGCTTCTTCCCTTACTATGGAGAGTTTTGCTTTCCTGTGGGCTGGAGGAAGAGCTTCCTACG
  GTGTGTCAAAAGGCAAAGTCTGCTTTGAGATGAAGGTAACAGAGAAGATTCCAGTAAGACACTTATATAC
  AAAAGATATTGATATACATGAAGTTCGGATTGGCTGGTCACTAACCACAAGTGGAATGTTGCTTGGTGAA
  GAAGAATTTTCTTACGGGTATTCTCTGAAAGGAATAAAAACATGCAACTGTGAGACAGAAGATTATGGGG
  AGAAGTTTGATGAAAATGATGTGATTACATGCTTTGCTAACTTTGAAACTGATGAAGTTGAACTCTCTTA
  TGCGAAGAATGGACAAGATCTTGGTGTTGCCTTTAAGATCAGTAAGGAAGTTCTTGCTGACCGGCCACTA
  TTCCCACATGTTCTCTGCCATAACTGTGCAGTTGAATTTAATTTCGGTCAAAAGGAAAAGCCATACTTTC
  CAATACCTGAAGACTGTACTTTTATCCAAAATGTCCCCTTAGAGGACCGAGTTAGAGGACCAAAAGGACC
  TGAAGAGAAGAAGGATTGTGAGGTTGTAATGATGATTGGCTTGCCAGGAGCTGGAAAAACTACCTGGGTT
  ACTAAACATGCAGCTGAAAACCCTGGGAAATACAACATTCTTGGAACAAATACGATTATGGACAAGATGA
  TGGTGGCAGGTTTTAAGAAGCAAATGGCAGATACTGGAAAACTGAACACACTGTTGCAGAGAGCCCCACA
  GTGTCTTGGCAAGTTTATTGAAATTGCTGCCCGTAAGAAGCGAAATTTTATTCTGGATCAGACAAATGTG
  TCTGCTGCTGCCCAGAGAAGAAAAATGTGCCTGTTTGCAGGCTTCCAGCGGAAAGCTGTTGTAGTGTGCC
  CAAAAGATGAAGACTATAAGCAGAGGACACAGAAGAAGGCAGAAGTAGAGGGGAAGGACCTACCAGAACA
  TGCTGTCCTCAAGATGAAAGGAAACTTCACCCTTCCAGAGGTTGCAGAATGCTTTGATGAAATAACCTAT
  GTTGAACTTCAGAAAGAGGAAGCCCAAAAGCTTTTGGAGCAATATAAAGAAGAAAGCAAAAAGGCACTGC
  CACCAGAAAAGAAGCAAAACACTGGCTCAAAGAAAAGCAATAAGAATAAGAGTGGCAAGAACCAGTTCAA
  CAGAGGTGGTGGCCATAGAGGCCGTGGAGGATTCAATATGCGAGGTGGCAATTTCAGAGGAGGAGCTCCT
  GGGAATCGTGGTGGATATAATAGGAGAGGCAACATGCCACAGAGAGGTGGTGGCGGTGGAAGTGGTGGAA
  TTGGCTATCCATACCCACGTGGCCCTGTTTTTCCTGGCCGAGGTGGTTACTCAAACAGAGGGAATTACAA
  CAGAGGTGGAATGCCCAACAGAGGGAACTATAACCAGAACTTCAGAGGACGAGGAAATAATCGTGGCTAC
  AAAAATCAATCTCAGGGCTACAATCAGTGGCAGCAGGGTCAATTCTGGGGTCAGAAGCCATGGAGTCAGC
  ATTATCACCAAGGATATTAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210713 representing NM_016805
  Red=Cloning site Green=Tags(s)
  
  MSSSPVNVKKLKVSELKEELKKRRLSDKGLKADLMDRLQAALDNEAGGRPAMEPGNGSLDLGGDAAGRSG
  AGLEQEAAAGAEDDEEEEGIAALDGDQMELGEENGAAGAADAGAMEEEEAASEDENGDDQGFQEGEDELG
  DEEEGAGDENGHGEQQSQPPAAAAQQQPSQQRGAGKEAAGKSSGPTSLFAVTVAPPGARQGQQQAGGDGK
  TEQKGGDKKRGVKRPREDHGRGYFEYIEENKYSRAKSPQPPVEEEDEHFDDTVVCLDTYNCDLHFKISRD
  RLSASSLTMESFAFLWAGGRASYGVSKGKVCFEMKVTEKIPVRHLYTKDIDIHEVRIGWSLTTSGMLLGE
  EEFSYGYSLKGIKTCNCETEDYGEKFDENDVITCFANFETDEVELSYAKNGQDLGVAFKISKEVLADRPL
  FPHVLCHNCAVEFNFGQKEKPYFPIPEDCTFIQNVPLEDRVRGPKGPEEKKDCEVVMMIGLPGAGKTTWV
  TKHAAENPGKYNILGTNTIMDKMMVAGFKKQMADTGKLNTLLQRAPQCLGKFIEIAARKKRNFILDQTNV
  SAAAQRRKMCLFAGFQRKAVVVCPKDEDYKQRTQKKAEVEGKDLPEHAVLKMKGNFTLPEVAECFDEITY
  VELQKEEAQKLLEQYKEESKKALPPEKKQNTGSKKSNKNKSGKNQFNRGGGHRGRGGFNMRGGNFRGGAP
  GNRGGYNRRGNMPQRGGGGGSGGIGYPYPRGPVFPGRGGYSNRGNYNRGGMPNRGNYNQNFRGRGNNRGY
  KNQSQGYNQWQQGQFWGQKPWSQHYHQGYY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016805
• ORF Size: 2400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016805.3
• RefSeq Size: 3670 bp
• RefSeq ORF: 2403 bp
• Locus ID: 51810
• UniProt ID: Q8VEK3
• Cytogenetics: 1 H4
• Gene Summary: DNA- and RNA-binding protein involved in several cellular processes such as nuclear chromatin organization, telomere-length regulation, transcription, mRNA alternative splicing and stability, Xist-mediated transcriptional silencing and mitotic cell progression (PubMed:20833368, PubMed:21235343, PubMed:22162999, PubMed:26244333). Plays a role in the regulation of interphase large-scale gene-rich chromatin organization through chromatin-associated RNAs (caRNAs) in a transcription-dependent manner, and thereby maintains genomic stability (By similarity). Required for the localization of the long non-coding Xist RNA on the inactive chromosome X (Xi) and the subsequent initiation and maintenance of X-linked transcriptional gene silencing during X-inactivation (PubMed:20833368, PubMed:26244333). Plays a role as a RNA polymerase II (Pol II) holoenzyme transcription regulator (PubMed:21235343, PubMed:22162999). Promotes transcription initiation by direct association with the core-TFIIH basal transcription factor complex for the assembly of a functional pre-initiation complex with Pol II in a actin-dependent manner. Blocks Pol II transcription elongation activity by inhibiting the C-terminal domain (CTD) phosphorylation of Pol II and dissociates from Pol II pre-initiation complex prior to productive transcription elongation. Positively regulates CBX5-induced transcriptional gene silencing and retention of CBX5 in the nucleus. Negatively regulates glucocorticoid-mediated transcriptional activation (By similarity). Key regulator of transcription initiation and elongation in embryonic stem cells upon leukemia inhibitory factor (LIF) signaling (PubMed:21235343). Involved in the long non-coding RNA H19-mediated Pol II transcriptional repression (By similarity). Participates in the circadian regulation of the core clock component ARNTL/BMAL1 transcription (PubMed:18332112). Plays a role in the regulation of telomere length. Plays a role as a global pre-mRNA alternative splicing modulator by regulating U2 small nuclear ribonucleoprotein (snRNP) biogenesis. Plays a role in mRNA stability. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Enhances the expression of specific genes, such as tumor necrosis factor TNFA, by regulating mRNA stability, possibly through binding to the 3'-untranslated region (UTR). Plays a role in mitotic cell cycle regulation. Involved in the formation of stable mitotic spindle microtubules (MTs) attachment to kinetochore, spindle organization and chromosome congression. Phosphorylation at Ser-58 by PLK1 is required for chromosome alignement and segregation and progression through mitosis. Contributes also to the targeting of AURKA to mitotic spindle MTs. Binds to double- and single-stranded DNA and RNA, poly(A), poly(C) and poly(G) oligoribonucleotides. Binds to chromatin-associated RNAs (caRNAs). Associates with chromatin to scaffold/matrix attachment region (S/MAR) elements in a chromatin-associated RNAs (caRNAs)-dependent manner (By similarity). Binds (via RNA-binding RGG-box region) to the long non-coding Xist RNA; this binding is direct and bridges the Xist RNA and the inactive chromosome X (Xi) (PubMed:20833368, PubMed:26244333). Binds the long non-coding H19 RNA. Binds to SMN1/2 pre-mRNAs at G/U-rich regions. Binds to small nuclear RNAs (snRNAs). Binds to the 3' UTR of TNFA mRNA (By similarity). Also negatively regulates embryonic stem cell differentiation upon LIF signaling (PubMed:21235343). Required for embryonic development (PubMed:16022389). Binds to brown fat long non-coding RNA 1 (Blnc1); facilitates the recruitment of Blnc1 by ZBTB7B required to drive brown and beige fat development and thermogenesis (PubMed:28784777).[UniProtKB/Swiss-Prot Function]