Plcd4 (NM_001081456) Mouse Tagged ORF Clone

CAT#: MG210581

Plcd4 (tGFP-tagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 1

Product Images

Circular map for MG210581

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plcd4
• Synonyms: 4921507K24Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210581 representing NM_001081456
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACATCTCAGATTCAAGACCTACTAGCCACTGATCAGGACCTGCTGCTGATGCAAGAAGGCACAATGA
  TGCGCAAGGTGAGGACCAAAAGCTGGAAGAAGCTAAGATACTTCAGACTTCAGAATGACGGCATGACAGT
  CTGGCATGGAAGTCAACCAGAAAGCATGCCCAAGCCCACTTTTTCGATCTCTGATGTGGAGAGGATACGT
  AAGGGGCAGGATTCTGAGTTGTTGCGCTATCTGGTGGAGGAGTTTCCCCTGGAGCAAGGGTTCACCGTTG
  TCTTTCATGGGCGCCGCCCCAACCTGGACCTAGTGGCCAACAGTGTTGAAGAGGCCCAGATTTGGATGCG
  AGGACTCCAGCTGTTGGTGGATCTTGTTGCCAGCATGGACCACCAGGAGCAAATGGATCAAATGTTGAAT
  GAGTGGTTCCAGCAAGCAGACAGAAACCAGGATGGCCGGATGAGTTTCAGAGAAGCTCAACGGCTGCTGC
  TTCTGATGAACGTGGAAATGGATGAAGAGTACGCCTTCAGTCTTTTCCAGGAGGCAGATGTTACCCAGTC
  TGATGATCTGGGGTCGGAAGAATTTGTACAGTTCTATAAGGCCCTGACTAAGCGCACTGAGATTGAAGAA
  ATATTTGAGGACTTCTCATCTGACAAACAGAAGCTGACCTTGCTGGAATTTGTGGATTTCCTTAGAAAGG
  AACAGAAAGAAAAAGACCATGCTCCTGACCTCGCTCTGGAACTCATTGACCGCTATGAGCCTTCTGAGAA
  TGGCCGATTGCTGCATGTGCTGAGCAAGGATGGCTTCCTCAAGTACCTCTGCTCGAAGGATGGAAACATC
  TTCAACAGTGACTGCCTCCCTATCTACCAGGATATGACTCAGCCTCTGAGCCACTACTACATTAACTCTT
  CTCACAACACCTACCTAGCGGGGGACCAGCTTTGCGGCCAGAGCAGCGTTGAAGGATACATACGGGCCTT
  GAAACGGGGGTGCCGCTGTGTGGAGGTGGACACGTGGGATGGACCTGATGGAGAGCCTGTTGTTTATCAT
  GGGCATACCCTGACCTCTCGCATCCTATTCAAAGATGTGTTGGCAACTCTCGCGCAATATGCCCTCCAGT
  CATCAGACTACCCTCTCATCTTGTCTCTGGAAAACCACTGCACCTGGGAACAGCAGAGGACCATGGCACA
  CCATCTGACCGAGATCCTGGGAGAGCAGCTGCTGAGGAACACCCTAGAGGGATTGCTGGTTGACAGCATG
  CCCTCGCCAGAGCAGCTTCGGGGGAAGATCTTGGTGAAAGGAAAGAAGTTAAGAACAATTGAAGTTGACA
  AGGAAGAAGAAGAAGAAGAAGAAGAAGAAGAGCTGGAGAAAGATGAGGGGCCAGATCTGGACCCAGCCTC
  CCCTGAGTTGGACACTCAACCTCAGCCTGAGACCCAGGGGCAGGCCGCTGGGAACAAGAAGGAGAGGAAG
  AAGAAGTTTCTTCTCCTGAGTTCCACAACCATCATGTGCCCAGACCTATCTGCCCTGGTTGTCTACTTGA
  GGACTGTCCCCTTCTGCAGCTTCACTCATTCCAAAGAAAACTACCACATCTATGACATTTCATCCTTTTC
  TGAGTCCAAGGCCAAGAACCTCATCAAGGAGGCAGGCAACGAGTTTGTACAGCACAATGCCAGACAGTTA
  TGCTGCGTGTATCCCAGTGGTCTGAGGACAGATTCATCCAACTTTAACCCCCAGGAACACTGGAATGTAG
  GCTGCCAGATGGTGGCTATGAATATGCAGACTGCAGGAAGTGCAATGGATATCTGTGATGGGCTCTTCCG
  CCAGAATGGTGGCTCTGGCTATGTGCTGAAGCCAGAATTCCTGAGAGATACCCAGAGTTCATTCAATCCT
  GAGAGGCCAATTAGTCTTTATAAGGCCCAGATCCTTGTAGTCCAGGTGATCAGTGGCCAACAACTCCCCA
  AAGTGGATAAAACCAAAGAGACAACCGTTGTGGATCCACTGGTGAAAGTAGAGCTCTATGGTGTTCCTGA
  AGACACAAAGGAGCAAGAGACCAGCCATGTGGAGAACAATGGTATTAATCCATACTGGGGGGAGACTTTC
  TATTTCCGGCTCCAGGTACCTGAACTTGCTATGTTGCGTTTTGTGGTAAAGGATTATAGCAGGAAATCCC
  GAAATAACTTTATTGGCCAATACACCCTACCTTGGACCTGTATGAAACAAGGCTACCGACATGTATCCTT
  GCTCTCCAGAGATGGCACTAGCCTCAATCCAGCTTCCATCTTTGTATACACCTGCATGCAGGAAGACCTG
  GATATGGATGAGCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210581 representing NM_001081456
  Red=Cloning site Green=Tags(s)
  
  MTSQIQDLLATDQDLLLMQEGTMMRKVRTKSWKKLRYFRLQNDGMTVWHGSQPESMPKPTFSISDVERIR
  KGQDSELLRYLVEEFPLEQGFTVVFHGRRPNLDLVANSVEEAQIWMRGLQLLVDLVASMDHQEQMDQMLN
  EWFQQADRNQDGRMSFREAQRLLLLMNVEMDEEYAFSLFQEADVTQSDDLGSEEFVQFYKALTKRTEIEE
  IFEDFSSDKQKLTLLEFVDFLRKEQKEKDHAPDLALELIDRYEPSENGRLLHVLSKDGFLKYLCSKDGNI
  FNSDCLPIYQDMTQPLSHYYINSSHNTYLAGDQLCGQSSVEGYIRALKRGCRCVEVDTWDGPDGEPVVYH
  GHTLTSRILFKDVLATLAQYALQSSDYPLILSLENHCTWEQQRTMAHHLTEILGEQLLRNTLEGLLVDSM
  PSPEQLRGKILVKGKKLRTIEVDKEEEEEEEEEELEKDEGPDLDPASPELDTQPQPETQGQAAGNKKERK
  KKFLLLSSTTIMCPDLSALVVYLRTVPFCSFTHSKENYHIYDISSFSESKAKNLIKEAGNEFVQHNARQL
  CCVYPSGLRTDSSNFNPQEHWNVGCQMVAMNMQTAGSAMDICDGLFRQNGGSGYVLKPEFLRDTQSSFNP
  ERPISLYKAQILVVQVISGQQLPKVDKTKETTVVDPLVKVELYGVPEDTKEQETSHVENNGINPYWGETF
  YFRLQVPELAMLRFVVKDYSRKSRNNFIGQYTLPWTCMKQGYRHVSLLSRDGTSLNPASIFVYTCMQEDL
  DMDEP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081456
• ORF Size: 2325 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081456.1, NP_001074925.1
• RefSeq Size: 2734 bp
• RefSeq ORF: 2328 bp
• Locus ID: 18802
• UniProt ID: Q8K3R3
• Cytogenetics: 1 38.54 cM
• Gene Summary: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.[UniProtKB/Swiss-Prot Function]