Ccpg1 (BC082602) Mouse Tagged ORF Clone

CAT#: MG210471

Ccpg1 (tGFP-tagged) - Mouse cell cycle progression 1 (cDNA clone MGC:105235 IMAGE:30663407)

Product Images

Circular map for MG210471

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccpg1
• Synonyms: 1700030B06Rik; 1810073J13Rik; 9430028F23Rik; AI426686; AI875170; CPR8; D9Ertd392e
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210471 representing BC082602
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAAAGTTCCAGCGACAGCGACTCATCTTGTGGCTGGACTGTCATCAATCATGAGGGTTCTGATA
  TAGAGATCGTGAATTCTGCCACAGCCAGTGACAACTGTGGACTGACTCTAGAATGTTCCTTAGTAGAACA
  AGAGGAGCTGCCCGTTCTTTATGTAGGGCATGGAGGTGAAGAAAGCAGCGCAAACAACACATCGTCAGTG
  GGAGAAACCATGTTGTCATCAATGAGAGAAACCAAGTCAGCAGCTGAGGTGGAGGAAGCACCTTCCCCTG
  AAGACAATGTCTATTTCGGAACTACTAGTGACGATTCGGATATTGTTACACTTGAACCACCTAAGCTGGA
  AGAAATGGGGAACCAGGAAGTTACAATTCAAGAAGCGCCCAGCTCAGATGACCTTAACATGGGCTCTTCC
  TCCAGCAGCCAGTACGCCTTCTGCCAGCCAGAACCCGTCTTTTCATCTCAGCCTAGTGATGAGGAATCCA
  GTAGCGATGACACCAGCCACGAGCCCAGCCCTGCTCCTAGACGGCGCCGCAACAGGAAGAAGACCGTTTC
  TATCTCAGAGTCTGAGGAGCCTCCACTCGCTGAGCCGGAAGATGAGCCCTCCAAGGAGCCAAGTAAACGC
  CACTTTAGTCGTGGTCTCAACAAGTGTGTTATCCTGGCCTTGGTGATTTCTGTCAGCATGGGATTTGGAC
  ATTTCTATGGCACAATCCAGATTCAGAAGCAGTTAGTCAGAAAGACCCACGAAGATGAGCTGGATGGTGT
  GAAGGGTTATCTCTCACAGCGCAAGCAGGAACAAGAGTCTTTTCTAGATTTCAAGTCGCTGAAAGAAAAC
  CTTGAGAGGTGTTGGACCGTTACTGAATCGAAGATTACCTTTGAAACTCAGAAGAAGAACCTTGCTGCAG
  AAAACCAGTATTTACGAATATCTCTGGAGAAGGAAGAACAAGCCCTGTCTTCATTACAGGAAGAGCTACG
  GCAACTAAGGGAGCAGATTAGATTGTTGGAGGATAAAGGCACAAGCACGCAGTTAGTTAGGGAGAACCAG
  GTCCTTAAGCAGTACTTAGAAGTGGAAAAGCAGAAAACAGATAGCTTCCTTAGGGAAAGGGAGATGCTCT
  TGGAGGAGGCAAGGATGCTGAAAAGGGACCTGGAGAGGGAACAGCTCACAGCCATGGCTCTGCGGGCAGA
  ACTTGAGCAGTTCATCCCTGGCCAGGCACAGAGCCGTGCGGAGTCTCCTAGTGTGCAGACTGAAGAGAAG
  GAAGTGGGGCTGTTACAGCAGAGGCTGGCTGAGCTGGAGCAGAAGCTCATCTTTGAGCAGCAGCGCTCGG
  ATTTGTGGGAGAGACTGTACGTTGAGGCAAAGGATCAACATGGAAAGCAGGAGACAGACGGGAGGAAGAG
  AGGAAGCAGAGGAAGCCACAGGGCGAAGAGCAAGTCGAAGGAGACATTTCTGGGGACAGTTAAGGAAACA
  TTTGATGCTATGAAGAATTCTACCAAGGAGTTTGTGAGACATCATAAAGAAAAAATCAAGCAGGCGAAAG
  AAGCTGTGAAGGAGAATCTGAAAAAATTCTCAGATTCAGTCAAATCCACTTTTCGCCATTTCAAAGATAC
  TACCAAGAATATCTTTGATGAAAAGGGCAGCAAGAGATTCAGGGCTCCAAAAGAAGCGGCAACTGAAAAG
  ACAAGAACAGCTTATAGTTATTCCTCATACTCACAGCAGGAGGCACCAAACCAAAACCAGAATTGCAGGC
  GCCCTTCTGCACAGAGGGATGGAGGGAGAGAAAAGCCCAGTCACTCTGAAGAAATTAGAAAGAATGCAAA
  TTCATATACATACAAGGCCAAGTCTGACTGCAGAGGCACCCATTCCACCAGAAGGGTTTGTTCAGGTATG
  TTTGAATGTGCTGGACAGGAAGCCATTAGCCCTGTTAATAAGGCGAGTCCTGTTAGGATGAATGACTTCA
  AACAGCTGATTCACTGGTACTTACTCAATGAACTGGAGACTTTTCACCACTGGAAAGAACTGGATCAGTT
  TATCAGTCCGTTTTTTTCGAATGGTGTCTTTAGACATGACCAGCAGCTCTTCGCCGACTTTGTTGATGAT
  GTAAAAGGTTACCTTAAGAGCATAAAGGAGTATCGAGTAGAGGATGGCAATCTGGAGAAGTTGGACGGAT
  GTATATATAGACACTTTGGGCATGTTTTTGCCCTGCCATTCGGACCCAGGTCGGTTTACATAAAACCATG
  TTACTATAATAGCTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210471 representing BC082602
  Red=Cloning site Green=Tags(s)
  
  MSESSSDSDSSCGWTVINHEGSDIEIVNSATASDNCGLTLECSLVEQEELPVLYVGHGGEESSANNTSSV
  GETMLSSMRETKSAAEVEEAPSPEDNVYFGTTSDDSDIVTLEPPKLEEMGNQEVTIQEAPSSDDLNMGSS
  SSSQYAFCQPEPVFSSQPSDEESSSDDTSHEPSPAPRRRRNRKKTVSISESEEPPLAEPEDEPSKEPSKR
  HFSRGLNKCVILALVISVSMGFGHFYGTIQIQKQLVRKTHEDELDGVKGYLSQRKQEQESFLDFKSLKEN
  LERCWTVTESKITFETQKKNLAAENQYLRISLEKEEQALSSLQEELRQLREQIRLLEDKGTSTQLVRENQ
  VLKQYLEVEKQKTDSFLREREMLLEEARMLKRDLEREQLTAMALRAELEQFIPGQAQSRAESPSVQTEEK
  EVGLLQQRLAELEQKLIFEQQRSDLWERLYVEAKDQHGKQETDGRKRGSRGSHRAKSKSKETFLGTVKET
  FDAMKNSTKEFVRHHKEKIKQAKEAVKENLKKFSDSVKSTFRHFKDTTKNIFDEKGSKRFRAPKEAATEK
  TRTAYSYSSYSQQEAPNQNQNCRRPSAQRDGGREKPSHSEEIRKNANSYTYKAKSDCRGTHSTRRVCSGM
  FECAGQEAISPVNKASPVRMNDFKQLIHWYLLNELETFHHWKELDQFISPFFSNGVFRHDQQLFADFVDD
  VKGYLKSIKEYRVEDGNLEKLDGCIYRHFGHVFALPFGPRSVYIKPCYYNSF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC082602
• ORF Size: 2258 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC082602, AAH82602
• RefSeq Size: 2931 bp
• RefSeq ORF: 2258 bp
• Locus ID: 72278
• Cytogenetics: 9 40.08 cM
• Gene Summary: Acts as an assembly platform for Rho protein signaling complexes. Limits guanine nucleotide exchange activity of MCF2L toward RHOA, which results in an inhibition of both its transcriptional activation ability and its transforming activity. Does not inhibit activity of MCF2L toward CDC42, or activity of MCF2 toward either RHOA or CDC42. May be involved in cell cycle regulation.[UniProtKB/Swiss-Prot Function]