Qsox1 (NM_001024945) Mouse Tagged ORF Clone

CAT#: MG210448

Qsox1 (tGFP-tagged) - Mouse quiescin Q6 (Qscn6), transcript variant 1

Product Images

Circular map for MG210448

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Qsox1
• Synonyms: 1300003H02Rik; Qscn6; QSOX; SOx
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210448 representing NM_001024945
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGAGGTGCGGCCGCCTCTCGGGGCCGCCATCGCTGCTGCTACTACTGCTGCTGCTCTCGCCTCTGC
  TCTTCTCGGGGCCCGGCGCTTACGCGGCCCGGCTCTCAGTGCTCTACTCGTCCTCTGACCCGCTGACGCT
  GCTGGATGCTGATTCGGTGCGTCCCACTGTGCTCGGCTCCAGCAGCGCCTGGGCGGTGGAGTTCTTCGCC
  TCCTGGTGTGGCCACTGCATCGCCTTCGCCCCGACGTGGAAGGAGCTTGCTAACGACGTGAAAGACTGGA
  GGCCAGCACTCAATCTTGCTGTCCTGGACTGTGCTGAGGAGACCAACAGTGCTGTCTGCAGAGAGTTCAA
  CATCGCTGGCTTCCCGACTGTGAGGTTTTTTCAGGCCTTTACCAAGAACGGTTCTGGAGCGACACTGCCA
  GGTGCTGGCGCTAATGTGCAGACTCTGCGTATGAGGCTCATCGATGCTCTGGAGTCCCACCGTGACACAT
  GGCCCCCAGCCTGTCCACCTCTGGAACCTGCCAAGCTGAATGATATCGACGGATTCTTTACAAGAAATAA
  AGCAGATTACCTGGCCCTGGTCTTTGAAAGGGAAGACTCCTACCTGGGTAGAGAGGTAACTCTGGACCTG
  TCCCAGTACCATGCTGTGGCAGTGCGCAGGGTCTTGAATACAGAGAGTGACCTGGTGAACAAGTTTGGCG
  TCACTGACTTCCCATCTTGTTACCTGCTGCTTCGGAATGGCTCTGTCTCCCGAGTGCCTGTGCTGGTGGA
  GTCCAGGTCTTTCTATACATCCTATCTTCGGGGGCTACCTGGACTGACCAGGGATGCTCCCCCAACCACA
  GCCACCCCAGTCACTGCTGATAAGATAGCACCCACAGTGTGGAAGTTTGCAGACCGCTCCAAGATCTACA
  TGGCCGACCTGGAGTCCGCACTCCACTACATCTTGCGTGTAGAAGTGGGGAAGTTCTCAGTGCTGGAGGG
  ACAGCGCCTGGTGGCCCTGAAAAAGTTTGTGGCAGTATTGGCCAAGTACTTCCCTGGGCAGCCTTTGGTC
  CAGAACTTCTTGCATTCCATAAACGACTGGCTTCAGAAGCAGCAGAAGAAGAGGATCCCCTACAGTTTCT
  TCAAAGCTGCTCTGGACAGCAGGAAGGAGGATGCTGTCCTTACTGAGAAGGTGAACTGGGTCGGCTGCCA
  GGGCAGTGAGCCACACTTCCGGGGGTTTCCCTGCTCACTGTGGGTCCTCTTCCACTTCCTGACGGTGCAG
  GCAAACCGATATAGTGAGGCCCACCCACAGGAACCAGCTGATGGCCAGGAGGTCCTCCAAGCCATGAGGA
  GCTATGTTCAGTTCTTCTTTGGCTGTCGTGACTGTGCGGACCATTTTGAGCAGATGGCTGCAGCGTCCAT
  GCACCAAGTGAGAAGTCCCAGTAATGCGATTCTTTGGCTCTGGACTAGCCACAACAGGGTTAACGCTCGC
  CTCTCAGGTGCTCTGAGTGAGGACCCCCACTTCCCCAAGGTGCAGTGGCCTCCCCGTGAGCTGTGTTCTG
  CCTGCCATAATGAACTCAACGGACAGGTGCCTTTGTGGGACCTTGGTGCCACCCTCAACTTTCTCAAGGC
  TCACTTCTCCCCAGCAAACATCGTCATAGACTCTTCTGCATCTAGACACACAGGCCGGAGAGGGAGTCCA
  GAAGCTACCCCCGAGCTGGTAATGGATACATTAAAACTGGAGAGCAGAAATTCAGTGTTGGGCCATGAGC
  AGGCTGCTTCTGCAGAGTCCCCTGGAGCCACTGCCCTAGATGTACCAGCTGAGAAGCCTGAAGCAAGTGG
  CCCCCAAGAACTATACACAGGCCTCAGAATGGGTGGAGCTTCACCAGGGCAGGGCCCTCCTGAGCGCATG
  GAAGACCACCAGAGGGATATGCAGGAGAATGCCCCCGGGCAGCAACACTTGAGCAAGAGAGACACTGAAG
  CCTTATTTCTGCCTGAGGTGAACCACCTCCAAGGCCCTTTAGAGCTCAGGCGAGGGGGCCGCAGCCCCAA
  GCAACTAGCCCCCATACTTGAAGAGGAACCAGAGGCCCTAGCTATACAGGGCCAAGGCCAGTGGCTGCAG
  GTTCTAGGAGGGGGCATTTCCCACCTGGACATTAGCCTCTGTGTGGGGCTCTACTCCGTGTCCTTCATGG
  GCTTACTGGCCATGTACACCTACTTTCGGGCCAGGCTGAGAACCCCAAAGGGCCATGCTAGTTACCCCAC
  AGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210448 representing NM_001024945
  Red=Cloning site Green=Tags(s)
  
  MRRCGRLSGPPSLLLLLLLLSPLLFSGPGAYAARLSVLYSSSDPLTLLDADSVRPTVLGSSSAWAVEFFA
  SWCGHCIAFAPTWKELANDVKDWRPALNLAVLDCAEETNSAVCREFNIAGFPTVRFFQAFTKNGSGATLP
  GAGANVQTLRMRLIDALESHRDTWPPACPPLEPAKLNDIDGFFTRNKADYLALVFEREDSYLGREVTLDL
  SQYHAVAVRRVLNTESDLVNKFGVTDFPSCYLLLRNGSVSRVPVLVESRSFYTSYLRGLPGLTRDAPPTT
  ATPVTADKIAPTVWKFADRSKIYMADLESALHYILRVEVGKFSVLEGQRLVALKKFVAVLAKYFPGQPLV
  QNFLHSINDWLQKQQKKRIPYSFFKAALDSRKEDAVLTEKVNWVGCQGSEPHFRGFPCSLWVLFHFLTVQ
  ANRYSEAHPQEPADGQEVLQAMRSYVQFFFGCRDCADHFEQMAAASMHQVRSPSNAILWLWTSHNRVNAR
  LSGALSEDPHFPKVQWPPRELCSACHNELNGQVPLWDLGATLNFLKAHFSPANIVIDSSASRHTGRRGSP
  EATPELVMDTLKLESRNSVLGHEQAASAESPGATALDVPAEKPEASGPQELYTGLRMGGASPGQGPPERM
  EDHQRDMQENAPGQQHLSKRDTEALFLPEVNHLQGPLELRRGGRSPKQLAPILEEEPEALAIQGQGQWLQ
  VLGGGISHLDISLCVGLYSVSFMGLLAMYTYFRARLRTPKGHASYPTA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001024945
• ORF Size: 2244 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001024945.1, NP_001020116.1
• RefSeq Size: 3399 bp
• RefSeq ORF: 2247 bp
• Locus ID: 104009
• UniProt ID: Q8BND5
• Cytogenetics: 1 G3
• Gene Summary: Catalyzes the oxidation of sulfhydryl groups in peptide and protein thiols to disulfides with the reduction of oxygen to hydrogen peroxide (PubMed:26819240). Plays a role in disulfide bond formation in a variety of extracellular proteins (PubMed:26819240). In fibroblasts, required for normal incorporation of laminin into the extracellular matrix, and thereby for normal cell-cell adhesion and cell migration (PubMed:26819240).[UniProtKB/Swiss-Prot Function]