Clcn5 (NM_016691) Mouse Tagged ORF Clone
CAT#: MG210435
- TrueORF®
Clcn5 (tGFP-tagged) - Mouse chloride channel 5 (Clcn5)
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_016691" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Clcn5 |
Synonyms | 5430408K11Rik; ClC-5; Clc4-1; Clc5; Clcn4-1; D930009B12Rik; DXImx42e; Sfc13; T25545 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210435 representing NM_016691
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTTCTTGGAGGAGCCAATCCCTGGTGTAGGGACCTATGATGATTTCAACACAATTGATTGGGTGA GAGAGAAATCTCGAGACCGAGATAGGCACCGAGAGATTACCAATAAAAGCAAAGAGTCAACATGGGCCTT AATTCACAGTGTGAGCGATGCTTTCTCTGGCTGGTTGTTAATGCTCCTTATTGGCCTTTTATCAGGTTCC TTAGCTGGCCTGATAGACATCTCTGCTCACTGGATGACAGACTTAAAAGAAGGGATATGTACAGGGGGGT TCTGGTTTAACCATGAGCACTGTTGCTGGAATTCTGAGCATGTTACCTTTGAACACAGAGACAAATGTCC AGAGTGGAATACCTGGGCCCAGCTCATCATCAATACAGATCAGGGAGCCTTTGCCTACATAGTCAATTAC TTCATGTACGTCCTCTGGGCTCTTCTGTTTGCTTTCCTTGCTGTATCTCTTGTCAAGGCATTTGCACCTT ATGCCTGTGGTTCTGGAATCCCTGAGATCAAAACCATCCTGAGTGGTTTCATTATTAGAGGCTATCTGGG TAAGTGGACCCTTGTCATCAAAACTATCACTTTGGTGTTGGCGGTGTCATCTGGCTTGAGCCTGGGCAAG GAGGGCCCCCTGGTGCACGTGGCTTGCTGCTGTGGGAACATCTTGTGCCACTGCTTCAACAAATACCGGA AGAATGAAGCCAAGCGGAGAGAGGTCTTATCAGCTGCAGCGGCTGCCGGTGTATCTGTAGCCTTTGGGGC CCCTATTGGTGGAGTATTATTCAGCCTGGAAGAGGTCAGCTACTACTTTCCCCTCAAAACACTATGGCGT TCTTTCTTCGCTGCTCTGGTGGCAGCGTTCACACTCCGCTCCATCAATCCATTTGGAAACAGCCGCTTAG TTCTGTTTTATGTAGAGTTCCACACCCCGTGGCATCTCTTTGAGCTCGTGCCATTCATTGTACTAGGCAT ATTCGGTGGCCTTTGGGGAGCTCTGTTTATCCGCACAAACATTGCCTGGTGTCGGAAGCGTAAAACCACA CAGTTGGGCAAGTATCCTGTCGTCGAGGTACTCATTGTGACGGCCATCACTGCCATCTTGGCTTTCCCCA ATGAATATACCCGGATGAGCACAAGTGAGCTCATTTCTGAGCTGTTCAATGACTGCGGCCTCCTGGACTC CTCCAAGCTCTGTGATTATGAGAACCACTTCAACACAAGCAAAGGCGGTGAGCTGCCTGACAGACCTGCT GGCGTGGGCGTCTACAGTGCCATGTGGCAGCTGGCTCTGACACTCATACTGAAAATAGTCATTACTATAT TCACCTTTGGCATGAAGATTCCTTCTGGTCTCTTTATCCCCAGCATGGCTGTCGGTGCTATAGCAGGCCG TCTTTTAGGAGTCGGAATGGAGCAACTGGCTTATTACCACCACGACTGGGGCATCTTCAATAGCTGGTGT AGTCAGGGAGCTGATTGTATCACCCCTGGCCTTTATGCCATGGTTGGGGCTGCTGCCTGCTTAGGTGGGG TGACTCGGATGACTGTTTCTCTTGTTGTCATAATGTTTGAACTGACTGGTGGCCTGGAATATATTGTGCC TCTGATGGCTGCAGCTATGACAAGCAAGTGGGTGGCTGATGCTCTTGGGCGAGAGGGCATTTATGATGCC CACATCCGTCTCAATGGATACCCCTTTCTTGAAGCCAAAGAAGAATTTGCTCATAAGACTCTGGCAATGG ATGTGATGAAGCCCCGGAGAAATGACCCCTTGCTGACTGTCCTTACTCAGGACAGTATGACTGTGGAGGA TGTCGAGACCATAATCAGCGAAACCACGTACAGTGGCTTTCCGGTGGTAGTGTCCCGAGAGTCGCAAAGA CTTGTAGGCTTTGTTCTTCGAAGAGATCTCATTATTTCAATTGAAAATGCTCGGAAGAAACAGGACGGGG TTGTGAGCACTTCCATCATTTATTTCACGGAGCATTCGCCTCCAATGCCTCCATACACCCCACCTACCCT GAAGCTTCGGAACATCCTGGACCTCAGCCCCTTCACTGTGACAGACCTTACGCCAATGGAGATCGTGGTG GACATCTTCCGCAAGCTGGGCCTGCGCCAGTGCCTGGTTACACACAACGGGCGCTTACTTGGAATCATCA CCAAAAAGGATGTGTTAAAGCATATAGCACAGATGGCAAACCAAGACCCTGATTCCATTCTCTTCAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210435 representing NM_016691
Red=Cloning site Green=Tags(s) MDFLEEPIPGVGTYDDFNTIDWVREKSRDRDRHREITNKSKESTWALIHSVSDAFSGWLLMLLIGLLSGS LAGLIDISAHWMTDLKEGICTGGFWFNHEHCCWNSEHVTFEHRDKCPEWNTWAQLIINTDQGAFAYIVNY FMYVLWALLFAFLAVSLVKAFAPYACGSGIPEIKTILSGFIIRGYLGKWTLVIKTITLVLAVSSGLSLGK EGPLVHVACCCGNILCHCFNKYRKNEAKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWR SFFAALVAAFTLRSINPFGNSRLVLFYVEFHTPWHLFELVPFIVLGIFGGLWGALFIRTNIAWCRKRKTT QLGKYPVVEVLIVTAITAILAFPNEYTRMSTSELISELFNDCGLLDSSKLCDYENHFNTSKGGELPDRPA GVGVYSAMWQLALTLILKIVITIFTFGMKIPSGLFIPSMAVGAIAGRLLGVGMEQLAYYHHDWGIFNSWC SQGADCITPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAMTSKWVADALGREGIYDA HIRLNGYPFLEAKEEFAHKTLAMDVMKPRRNDPLLTVLTQDSMTVEDVETIISETTYSGFPVVVSRESQR LVGFVLRRDLIISIENARKKQDGVVSTSIIYFTEHSPPMPPYTPPTLKLRNILDLSPFTVTDLTPMEIVV DIFRKLGLRQCLVTHNGRLLGIITKKDVLKHIAQMANQDPDSILFN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016691 |
ORF Size | 2238 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016691.2, NP_057900.2 |
RefSeq Size | 2749 bp |
RefSeq ORF | 2241 bp |
Locus ID | 12728 |
UniProt ID | Q9WVD4 |
Cytogenetics | X 3.21 cM |
Gene Summary | Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202208 | Clcn5 (untagged) - Mouse chloride channel 5 (Clcn5), (10ug) |
USD 684.00 |
|
MR210435 | Clcn5 (Myc-DDK-tagged) - Mouse chloride channel 5 (Clcn5) |
USD 684.00 |
|
MR210435L3 | Lenti ORF clone of Clcn5 (Myc-DDK-tagged) - Mouse chloride channel 5 (Clcn5) |
USD 984.00 |
|
MR210435L4 | Lenti ORF clone of Clcn5 (mGFP-tagged) - Mouse chloride channel 5 (Clcn5) |
USD 984.00 |
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