Anapc5 (NM_001042491) Mouse Tagged ORF Clone
CAT#: MG210311
- TrueORF®
Anapc5 (tGFP-tagged) - Mouse anaphase-promoting complex subunit 5 (Anapc5), transcript variant 2
ORF Plasmid: DDK
Lentiviral Particles: DDK w/ Puro mGFP w/ Puro
"NM_001042491" in other vectors (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Anapc5 |
Synonyms | 2510006G12Rik; AA408751; AA536819; AA986414; Anpc5; APC5 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210311 representing NM_001042491
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGACCAACGGGGTAGTGCACGCCAACTTGTTTGGCATCAAGGACTGGGTGACGCCCTATAAGATCG CCGTCCTGGTGCTGCTCAACGAGATGGGCCGCACGGGCGAGGGCGCCGTCAGCCTCGTGGAGCGGCGGAA GCTCAACCAGCTGCTCCTGCCCCTGCTGCAGGGCCCAGATATTACACTGTCAAAGCTGTACAAGCTAATT GAAGAATCGTGTCCTCAGCTGGCAAATTCAGTGCAGATCAGAATCAAGCTCATGGCTGAAGGCGAACTGA AGGATATGGAACAATTTTTTGATGACCTTTCAGATTCTTTTTCTGGAACTGAACCAGAGGTTCACAAAAC GAGTGTAGTAGGTCTGTTCCTGCGCCACATGATCTTGGCCTACAGTAAGCTTTCTTTCAGTCAAGTGTTT AAGCTGTACACTGCCCTCCAGCAGTACTTCCAGAACGGGGAGAAAAAGACGGTGGAAGATGCTGATATGG ACCGAGAGGATGGAGAGAAACAGATGGAGAAGGAAGAGCTCGACGTGTCCGTGAGAGAAGAGGAAGTATC TTGCAGTGGTCCTCTGTCCCAAAAACAAGCGGAATTTTTTCTCTCTCAGCAGGCCGCTTTGTTGAAGAAT GATGAGACTAAAGCCCTCACCCCAGCTTCCTTGCAGAAGGAATTGAACAACCTGTTGAAGTTTAATCCTG ATTTTGCTGAAGCTCATTACCTCAGCTACTTAAACAACCTCCGTGTTCAAGATGTTTTCAGCTCAACACA CAGCCTCCTGCATTATTTTGACCGCCTGATTCTCACTGGAGCGGAGGGCAAAAGTAATGGGGAAGAGGGT TATGGCCGGAGCCTGAGATACGCTGCTCTCAACCTGGCTGCCCTGCACTGCCGCTTCGGTCACTATCAAC AGGCAGAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCAGGAGTCCAACGATCACGTGTGTCTGCAGCA CTGTTTGAGCTGGCTTTATGTGCTGGGGCAGAAGAGAGCCGATAGCTATGTTCTGCTGGAGCACTCTGTG AAGAAAGCAGTACATTTTGGGTTACCGAGAGCTTTTGCTGGGAAGACGGCCAACAAACTGATGGATGCCC TAAAGGACTCTGACCTCCTGCACTGGAAACACAGCCTGTCAGAACTTATCGATATCAGCATTGCACAGAA AACGGCCATCTGGAGGCTGTACGGCCGCAGCACCATGGCACTGCAACAAGCCCAGATGTTGCTGAGCATG AACAGCCTGGAGTCGCTGAATGCGGGTGTACAGCAGAACAATACTGAGTCCTTTGCCGTCGCTCTCTGCC ATCTTGCAGAGCTCCATGCAGAACAGGGCTGTTTTGCGGCTGCTGGTGAAGTATTAAAGCACTTGAAGGA CCGATTTCCACCCAACAGTCAGCACGCCCAGTTATGGATGCTGTGTGATCAAAAAATACAGTTTGACAGA GCAATGAATGATGGCAAATTCCATTTGGCTGATTCACTTGTTACAGGAATCACAGCGCTTAATGGCATAG AAGGTGTATACAGGAAAGCAGTCGTACTGCAGGCTCAGAACCAAATGACAGAGGCACACAAGCTACTGCA GAAGTTGCTGACGTACTGTCAGAAGTTAAAGAACACAGAAATGGTCATCAGCGTCCTCCTATCGGTGGCA GAGCTGTACTGGCGATCTTCGTCCCCGACCATTGCCATGCCTGTGCTCCTGGAAGCTCTGGCCCTCTCCA AAGAATACCGATTGCAGTACTTGGCCTCCGAAACTGTGCTCAACTTGGCTTATGCCCAGCTCATCCTTGG AATCCCAGAACAGGCCTTAACCCTTCTCCACATGGCTATCGAGCCCATCCTAGCTGACGGGGCTATCCTG GACAAAGGTCGTGCCATGTTCTTAGTGTCCAAGTGCCAAGTGGCTTCGGCAGCGTCCTATGACCCAGTGA AGAAAGCGGAAGCTCTGGAAGCGGCCGTTCAGAACCTCAGTGAAGCCAAGAACTACTTTGCACAAGTCGA CTGCAGAGAGCGCATCAGGGATGTTGCTTACTTCCAGGCCAGGCTGTACCACGCTCTTGGCAAGACCCAG GAGAGGAACCATTGTGCCATGATCTTCCGGCAGCTGCACCAGGAGTTGCCCGCCCATGGGGTGCCCTTGA TTAACCACCTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210311 representing NM_001042491
Red=Cloning site Green=Tags(s) MMTNGVVHANLFGIKDWVTPYKIAVLVLLNEMGRTGEGAVSLVERRKLNQLLLPLLQGPDITLSKLYKLI EESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVF KLYTALQQYFQNGEKKTVEDADMDREDGEKQMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQAALLKN DETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAEGKSNGEEG YGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRADSYVLLEHSV KKAVHFGLPRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRSTMALQQAQMLLSM NSLESLNAGVQQNNTESFAVALCHLAELHAEQGCFAAAGEVLKHLKDRFPPNSQHAQLWMLCDQKIQFDR AMNDGKFHLADSLVTGITALNGIEGVYRKAVVLQAQNQMTEAHKLLQKLLTYCQKLKNTEMVISVLLSVA ELYWRSSSPTIAMPVLLEALALSKEYRLQYLASETVLNLAYAQLILGIPEQALTLLHMAIEPILADGAIL DKGRAMFLVSKCQVASAASYDPVKKAEALEAAVQNLSEAKNYFAQVDCRERIRDVAYFQARLYHALGKTQ ERNHCAMIFRQLHQELPAHGVPLINHL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001042491 |
ORF Size | 2181 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001042491.1, NP_001035956.1 |
RefSeq Size | 2721 bp |
RefSeq ORF | 2184 bp |
Locus ID | 59008 |
UniProt ID | Q8BTZ4 |
Cytogenetics | 5 F |
Gene Summary | Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC202371 | Anapc5 (untagged) - Mouse anaphase-promoting complex subunit 5 (Anapc5), transcript variant 2, (10ug) |
USD 667.00 |
|
MR210311 | Anapc5 (Myc-DDK-tagged) - Mouse anaphase-promoting complex subunit 5 (Anapc5), transcript variant 2 |
USD 667.00 |
|
MR210311L3 | Lenti ORF clone of Anapc5 (Myc-DDK-tagged) - Mouse anaphase-promoting complex subunit 5 (Anapc5), transcript variant 2 |
USD 967.00 |
|
MR210311L4 | Lenti ORF clone of Anapc5 (mGFP-tagged) - Mouse anaphase-promoting complex subunit 5 (Anapc5), transcript variant 2 |
USD 967.00 |
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