Brd3 (NM_023336) Mouse Tagged ORF Clone
CAT#: MG210310
- TrueORF®
Brd3 (tGFP-tagged) - Mouse bromodomain containing 3 (Brd3)
"NM_023336" in other vectors (5)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Symbol | Brd3 |
Synonyms | 2410084F24Rik; AW060456; Fsrg2; ORFX; RINGL3 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG210310 representing NM_023336
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCACTACAGCGGCTGCCCCCACGGGGATCCCGGCAGTCCCGGGCCCCGTGAACCCTCCCCCACCTG AGGTCTCCAACCCCAGCAAGCCCGGGCGAAAGACTAACCAACTGCAGTACATGCAGAATGTAGTGGTGAA GACACTCTGGAAACATCAGTTTGCCTGGCCTTTCTACCAGCCTGTGGATGCAATCAAGCTGAACCTGCCT GATTATCATAAAATAATAAAAAACCCAATGGACATGGGGACTATCAAGAAGAGACTAGAAAATAATTATT ATTGGAGTGCCAGTGAGTGTATGCAGGACTTCAACACCATGTTTACAAACTGTTATATTTATAATAAGCC CACAGATGATATAGTGCTAATGGCCCAGGCCTTAGAGAAAATCTTTCTGCAGAAAGTGGCCCAGATGCCT CAGGAGGAAGTTGAACTATTGCCCCCTGCTCCAAAGGGCAAAGGCCGGAAGCCAGCTGCAGGAGCCCAAA ATGCAGGTTCCCAACAAGTGGCAGCTGTGTCTTCGGTCTCCCCAGCAACCCCCTTCCAGAACATACCCCC CACCGTGTCCCAGACACCCGTCATTGCCGCCACCCCTGTACCAACCATCACTGCAAACGTCACGTCAGTT CCAGTCCCCCCACCCACTGCACCGCCTCCTCCTGCGACACCCATCGTCCCTGTGGTCCCTCCCACACCGC CTGTAGTCAAGAAAAAGGGCGTGAAGCGGAAAGCAGACACAACCACACCCACAACATCTGCCATCACTGC CAGCCGGAGTGAGTCTCCCCCGCCACTTTCAGAGCCCAAGCAAGCCAAGGTAGTGGCCCGAAGGGAGAGC GGGGGCCGCCCCATCAAACCTCCCAAGAAGGACCTGGAAGATGGTGAGGTCCCACAGCACGCGGGTAAAA AGGGAAAACTGTCCGAGCACCTGCGGCACTGTGACAGCATCCTCCGGGAGATGCTGTCCAAGAAGCATGC TGCCTATGCGTGGCCCTTTTACAAGCCAGTGGACGCGGAGGCGCTGGAGCTGCATGACTACCATGACATC ATCAAGCACCCCATGGACCTCAGCACGGTCAAAAGGAAGATGGATAGCCGAGAGTACCCAGATGCACAGG GCTTTGCTGCTGATATCCGGTTAATGTTCTCGAATTGTTATAAGTACAACCCTCCAGACCACGAAGTGGT GGCCATGGCCAGGAAGCTCCAGGATGTGTTTGAGATGAGGTTTGCCAAGATGCCCGATGAGCCCATGGAG GCACCTGCGCTGCCCGCTCCCACGGCCCCCATCGTGAGCAAGGGGGCTGAGAGCAGCCGCAGTAGTGAGG AGAGCTCTTCAGATTCAGGCAGCTCAGACTCAGAAGAGGAGCGAGCCACTCGGCTGGCTGAGCTGCAGGA GCAGCTGAAGGCCGTGCACGAACAGCTGGCTGCCTTGTCTCAGGCCCCAGTGAACAAACCAAAGAAGAAG AAAGAGAAGAAGGAAAAGGAAAAGAAGAAGAAAGACAAGGACAAAGACAAAGAGAAGGAGAAGCACAAGG CCAAGTCTGAGGAGGAGAAGAAGGCCAAGGCAGCCCCAGCAGCCAAGCAGGCCCAGCAGAAAAAGGCTCC CACCAAGAAGGCCAACAGCACAACCACAGCCAGCAGACAGCTCAAGAAGGGCGGCAAGCAGGCATCTGCG TCCTATGACTCAGAGGAAGAGGAGGAGGGCCTGCCCATGAGCTATGATGAAAAGCGACAACTTAGCCTTG ACATCAACCGGCTGCCCGGCGAGAAGCTAGGGCGTGTGGTGCACATCATTCAGTCTCGGGAGCCCTCGCT TCGGGACTCAAACCCAGACGAGATTGAGATTGACTTTGAGACCCTGAAGCCAACCACGCTGCGGGAACTG GAGAGATATGTCAAGTCTTGTTTACAAAAAAAGCAGAGGAAACCATTGTCAACAAGCGGGAAGAAGCAGG CAGCCAAATCGAAAGAGGAGCTAGCTCAGGAGAAGAAAAAGGAGCTGGAGAAGCGGCTGCAGGATGTCAG TGGACAACTGAACAGCAAAAAACCCACTAAGAAAGAGAAGTCCGGCTCAGCTCCCTCAGGAGGCCCATCA AGGCTCAGCAGTAGCAGCTCCTCTGAGTCTGCGAGCAGTAGCTCCAGTGGATCGAGCTCCGACAGCAGTG ACTCAGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210310 representing NM_023336
Red=Cloning site Green=Tags(s) MSTTAAAPTGIPAVPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLP DYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMP QEEVELLPPAPKGKGRKPAAGAQNAGSQQVAAVSSVSPATPFQNIPPTVSQTPVIAATPVPTITANVTSV PVPPPTAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSEPKQAKVVARRES GGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRHCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDI IKHPMDLSTVKRKMDSREYPDAQGFAADIRLMFSNCYKYNPPDHEVVAMARKLQDVFEMRFAKMPDEPME APALPAPTAPIVSKGAESSRSSEESSSDSGSSDSEEERATRLAELQEQLKAVHEQLAALSQAPVNKPKKK KEKKEKEKKKKDKDKDKEKEKHKAKSEEEKKAKAAPAAKQAQQKKAPTKKANSTTTASRQLKKGGKQASA SYDSEEEEEGLPMSYDEKRQLSLDINRLPGEKLGRVVHIIQSREPSLRDSNPDEIEIDFETLKPTTLREL ERYVKSCLQKKQRKPLSTSGKKQAAKSKEELAQEKKKELEKRLQDVSGQLNSKKPTKKEKSGSAPSGGPS RLSSSSSSESASSSSSGSSSDSSDSE TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023336 |
ORF Size | 5426 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023336.3 |
RefSeq Size | 3033 bp |
RefSeq ORF | 2181 bp |
Locus ID | 67382 |
UniProt ID | Q8K2F0 |
Cytogenetics | 2 A3 |
Gene Summary | Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors (PubMed:21536911). Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (PubMed:21536911).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC205406 | Brd3 (untagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 3, (10ug) |
USD 665.00 |
|
MG223345 | Brd3 (tGFP-tagged) - Mouse bromodomain containing 3 (Brd3) transcript variant 3, (10ug) |
USD 865.00 |
|
MR223345 | Brd3 (Myc-DDK-tagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 3 |
USD 665.00 |
|
MR223345L3 | Lenti ORF clone of Brd3 (Myc-DDK-tagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 3 |
USD 965.00 |
|
MR223345L4 | Lenti ORF clone of Brd3 (mGFP-tagged) - Mouse bromodomain containing 3 (Brd3), transcript variant 3 |
USD 965.00 |
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