E430025E21Rik (BC034070) Mouse Tagged ORF Clone

CAT#: MG210187

E430025E21Rik (tGFP-tagged) - Mouse RIKEN cDNA E430025E21 gene (cDNA clone MGC:31278 IMAGE:4217923)

Product Images

Circular map for MG210187

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: E430025E21Rik
• Synonyms: MGC31278, mKIAA0196
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210187 representing BC034070
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGCCGGTGTGGAGGTGTGGGTGCAAGGCCTCTGGGCAGGAAGAGGAGTTGGAAAATCTTCAAG
  CTTGGTTCAGAGAGATCTCAAAACAAATACTGTCTCTAAATTATGATGATTCTACTGCTGCAGGCAGAAA
  AACTGTGCAACTAATACAAGCTCTGGAAGAGGTTCAGGAGTTTCACCAGTTGGAATCAAATCTACAAGTT
  TGCCAGTTTCTAGCTGACACCCGAAAGTTTCTCCATCAAATGATCAGAACCATCAACATCAAGGAGGAAG
  TCCTGATCACAGTGCAGATCATCGGGGACCTTTCCTTTGCCTGGCAGTTAATTGACAGTTTCACATCCAT
  CATGCAAGAAAGCATAAGGGTAAACCCATCCATGGTTACCAAACTCAGAGCCACGTTCCTAAAGCTTGCC
  TCTGCCCTTGACCTTCCCCTGCTACGTATTAATCAAGCAAATAGCCCTGATCTCCTCAGTGTGTCTCAGT
  ATTATTCAGGAGAATTGGTGTCATACGTGAGAAAGGTTTTGCAGATCATTCCAGAAAGCATGTTCACATC
  TCTCCTGAAGATCATAAAGCTTCAGACCCATGACATCATGGAAGTGCCTACACGCCTGGACAAGGACAAG
  CTGCGGGACTACGCTCAGCTTGGCCCGAGATATGAGGTTGCCAAGCTCACTCACGCCATCTCCATCTTTA
  CTGAGGGCATCTTGATGATGAAAACGACCCTGGTGGGCATCATCAAGGTGGATCCCAAGCAGTTGCTGGA
  AGACGGAATAAGGAAAGAGCTGGTTAAACGCGTGGCTTTCGCCCTTCACCGGGGACTGATATTCAACCCT
  CGTGCCAAGCCAAGTGAGTTGATGCCCAAACTGAAAGAGCTGGGGGCCACCATGGATGGGTTCCATCGCT
  CCTTTGAGTATATACAGGACTACGTCAGCATTTACGGTCTGAAGATTTGGCAGGAGGAAGTCTCTCGTAT
  TATAAATTATAACGTGGAACAGGAGTGTAATAACTTCTTAAGAACAAAGATTCAAGACTGGCAGAGTATG
  TACCAATCCACTCATATACCAATCCCGAAGTTTGCACCTGTGGATGAGTCTATAACATTTATTGGTCGAC
  TCTGCAGAGAAATCTTGCGGATCACAGACCCAAAAATGACATGTTACATAGACCAGCTGAACACTTGGTA
  TGACGTGAAAACTCATCAAGAAGTGACCAGCAGTCGCCTCTTCTCAGAAATCCAGACCACCTTGGGGACT
  TTTGGTCTGAATGGATTGGACAGGCTTCTGTGCTTCATGATTGTGAAAGAGCTACAGAATTTCCTCAGTA
  TGTTTCAGAAAATTATCCTGAAAGAGAGAACTGTTCAAGAAACTTTAAAGATGCTCATGAGTGCTGTTAA
  CCCTCTAAAGAGCATAGTGGCAAATTCAAGTAAAGTTTATCTTTCTGCCATCACCAAAACACAGAAGATC
  TGGAGTGCTTACCTGGAGGCTATAATGAAGGTTGGACAGATGCAGATCCTGAGACAACAGATCGCCAATG
  AGCTAAACTCTTCCTGTCGATTTGATTCCAGACATCTGGCAGCTGCCCTGGATAATCTCAATAAGGCTCT
  CCTGGCAGACATCGAAGCCCACTACCGGGACCCTTCGCTTCCTTACCCCAAAGAAGATAACACACTCTTA
  TATGAAATCACAGCCTATCTGGAGGCGGCTGGCATTCACAACCCCCTGAATAAGATTTACATAACAACGA
  AGCGCTTACCCTATTTCCCGATTGTCAACTTTCTGTTTTTGATCGCGCAGTTGCCAAAACTTCAGTATAA
  CAAAAATCTAGGCATGGTCTGCCGGAAGCCTGCCGACCCTGTTGACTGGCCACCGCTCGTCCTAGGTCTG
  CTCACGCTGCTGAAGCAGTTCCACTCCCGGTACACCGAGCAATTCCTGGCACTGATTGGCCAGTTTATTC
  GCTCCACAATGGAGCAGTGCACAAGCCAGAAGATGCCTGAAATGCCTGCAGATGCTGTGGGCGCCCTTCT
  CTTCCTGGAGGATTATGTTCGGTATACAAAACTCCCCCGAAGGGTGGCTGAAGCCCATGTGCCTAACTTC
  ATTTTTGATGAATTCCGAACAGTCCTG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210187 representing BC034070
  Red=Cloning site Green=Tags(s)
  
  MAEPVWRCGCKASGQEEELENLQAWFREISKQILSLNYDDSTAAGRKTVQLIQALEEVQEFHQLESNLQV
  CQFLADTRKFLHQMIRTINIKEEVLITVQIIGDLSFAWQLIDSFTSIMQESIRVNPSMVTKLRATFLKLA
  SALDLPLLRINQANSPDLLSVSQYYSGELVSYVRKVLQIIPESMFTSLLKIIKLQTHDIMEVPTRLDKDK
  LRDYAQLGPRYEVAKLTHAISIFTEGILMMKTTLVGIIKVDPKQLLEDGIRKELVKRVAFALHRGLIFNP
  RAKPSELMPKLKELGATMDGFHRSFEYIQDYVSIYGLKIWQEEVSRIINYNVEQECNNFLRTKIQDWQSM
  YQSTHIPIPKFAPVDESITFIGRLCREILRITDPKMTCYIDQLNTWYDVKTHQEVTSSRLFSEIQTTLGT
  FGLNGLDRLLCFMIVKELQNFLSMFQKIILKERTVQETLKMLMSAVNPLKSIVANSSKVYLSAITKTQKI
  WSAYLEAIMKVGQMQILRQQIANELNSSCRFDSRHLAAALDNLNKALLADIEAHYRDPSLPYPKEDNTLL
  YEITAYLEAAGIHNPLNKIYITTKRLPYFPIVNFLFLIAQLPKLQYNKNLGMVCRKPADPVDWPPLVLGL
  LTLLKQFHSRYTEQFLALIGQFIRSTMEQCTSQKMPEMPADAVGALLFLEDYVRYTKLPRRVAEAHVPNF
  IFDEFRTVL
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC034070
• ORF Size: 2127 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC034070.1
• RefSeq Size: 2642 bp
• RefSeq ORF: 2129 bp
• Locus ID: 223593
• Cytogenetics: 15 D1
• Gene Summary: Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting. May be involved in axonal outgrowth. Involved in cellular localization of ADRB2. Involved in cellular trafficking of BLOC-1 complex cargos such as ATP7A and VAMP7 (By similarity). Involved in cytokinesis and following polar body extrusion during oocyte meiotic maturation (PubMed:24998208).[UniProtKB/Swiss-Prot Function]