Gyltl1b (BC096655) Mouse Tagged ORF Clone

CAT#: MG210059

Gyltl1b (tGFP-tagged) - Mouse glycosyltransferase-like 1B (cDNA clone MGC:106915 IMAGE:5057489)

Product Images

Circular map for MG210059

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Gyltl1b
• Synonyms: Largel, mKIAA4105
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210059 representing BC096655
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCCGAGGTCGTCCCCGGGCAATGGGGGCCGCCGTGCTGCTGCTGCTGCTGTTGCTAGTGGTTG
  GCTTCTTCCTGTTCGGCCGGGACCCGGATTATGGACTGGGCACAACTGCTACCCTCGATGAAGACCCGTA
  CAGGAGTCGCAACCTCTCCGCCTCCAGCCCGCAGCTTCTACTGCCACCCAAGTGCGAGATGTTGCATGTG
  GCTATCGTGTGTGCGGGATACAACTCCAGCCGAGAGATTATTACCCTAACGAAGTCCCTGCTATTCTACA
  GGAAAAATCCGCTGCACCTCCACCTGATAACTGATGCCGTAGCCAGAAACATCCTGGAGACACTCTTCCG
  AACATGGATGGTGCCAGCGGTGGTGGTCAGCTTCTATGATGCGGAAGAACTCAAGCCCCTGGTCTCCTGG
  ATCCCCAACAAACACTACTCTGGCCTCTATGGGCTAATGAAGCTAGTACTTCCCAGCATCCTGCCTCCCA
  GCCTGGCCCGAGTCATCGTCCTGGATACCGACGTCACTTTCTCCTCTGACATTGTGGAGCTCTGGGCACT
  CTTTGATCATTTTTCTGACAAGCAGGTGGTCGGTCTCGTGGAGAACCAGAGCGACTGGTACCTGGGCAAC
  CTCTGGAAGAACCATAGGCCCTGGCCTGCCTTGGGCAGGGGATTTAACACAGGTGTGATCCTGCTGTGGC
  TGGACAGGCTCCAGCAAACTGGCTGGGAGCAGATGTGGAAGGTGACAGCCAAACGAGAGCTGCTCACTCT
  GATGGCTACTTCCTTGGCTGACCAGGACATCTTCAATGCGGTCATCAAGGAGCACCCCCATCTGGTGCAC
  CCCCTGCCCTGTGTCTGGAACGTGCAGCTGTCAGACCACACTCGGGCTGAGCGCTGCTACCTGGAAGCAG
  CTGACCTCAAAGTGATCCACTGGAATTCACCAAAGAAGCTTCGAGTGAAGAACAAGCACGCAGAATTCTT
  CCGTAATCTGCACTTGACCTTTCTGGGGTATGATGGGAAGCTACTGCGAAGAGAGCTCTTTGGATGCCCC
  AACCAGTTCCCTCCTGGGGCCGAGCAGTTGCAACAGGCCCTAACACAGCTGGATGAGGAAGAGCCCTGCT
  TTGAGTTCCGCCAACAGCAGCTCACTGTGCACCGGGTGCACATCACCTTCCTGCCCCACCAGCCGCCACC
  TCCCCAGCCTCACGATGTCACCTTGGTGGCCCAACTCTCTATGGACCGGCTGCAGATGCTGGAAGCCCTG
  TGCAGGCACTGGCCAGGCCCCATGAGCCTGGCCTTGTACCTGACAGATGAAGAGGCTCAACAATTTCTTC
  ATTTTGTGGAAACGTCGCCAGTGCTCTCTATGAGGAAGGATGTGGCCTACCATGTAGTGTACCGGGACGG
  TCCACTCTATCCAGTCAACCAGCTCCGCAACGTGGCCTTGGCCCAGGCTCTCACACCCTACGTCTTCCTC
  AGTGATATTGACTTCTTACCTGCCTACTCCCTCTACGACTACCTCAGGGCTTCTATCGAGCAGCTGGAGC
  TGGACAGTCGGCGCAAGACTGCTTTGGTGGTGCCTGCATTTGAGACCCTACACTACCGGTTCAGCTTCCC
  AAACTCTAAGGCAGAGCTGTTGACGTTACTGGATGCCGGCTCCCTTCACACCTTTAGGTACCACGAGTGG
  CCACAGGGTCACTCATCCACAGACTATTCCCGCTGGCGGGAAGCCCAGGCACCATACAGTGTGCAGTGGT
  CAGCTGACTATGAACCCTACGTGGTGGTACCCCGTGACTGCCCCCGTTATGATCCTCGCTTTGTGGGATT
  TGGCTGGAACAAGGTGGCCCACATCATAGAGTTGGATGCTCAGGAATATGAATTCCTGGTACTTCCTGAG
  GCCTTCTCTATCCACTTGCCCCACGCTCCAAGTCTTGACATCTCCCGCTTCCGCTCCAGCCCCACCTACC
  GCAACTGTCTCCAGGCCCTCAAGGAAGAGTTCCACCAGGACTTGTCAAGGCGCTATGGGTCTGCAGCCCT
  GAAATACCTCACTGCCCTGCAGCAGGCCCGAAGTCGGGCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210059 representing BC096655
  Red=Cloning site Green=Tags(s)
  
  MLPRGRPRAMGAAVLLLLLLLVVGFFLFGRDPDYGLGTTATLDEDPYRSRNLSASSPQLLLPPKCEMLHV
  AIVCAGYNSSREIITLTKSLLFYRKNPLHLHLITDAVARNILETLFRTWMVPAVVVSFYDAEELKPLVSW
  IPNKHYSGLYGLMKLVLPSILPPSLARVIVLDTDVTFSSDIVELWALFDHFSDKQVVGLVENQSDWYLGN
  LWKNHRPWPALGRGFNTGVILLWLDRLQQTGWEQMWKVTAKRELLTLMATSLADQDIFNAVIKEHPHLVH
  PLPCVWNVQLSDHTRAERCYLEAADLKVIHWNSPKKLRVKNKHAEFFRNLHLTFLGYDGKLLRRELFGCP
  NQFPPGAEQLQQALTQLDEEEPCFEFRQQQLTVHRVHITFLPHQPPPPQPHDVTLVAQLSMDRLQMLEAL
  CRHWPGPMSLALYLTDEEAQQFLHFVETSPVLSMRKDVAYHVVYRDGPLYPVNQLRNVALAQALTPYVFL
  SDIDFLPAYSLYDYLRASIEQLELDSRRKTALVVPAFETLHYRFSFPNSKAELLTLLDAGSLHTFRYHEW
  PQGHSSTDYSRWREAQAPYSVQWSADYEPYVVVPRDCPRYDPRFVGFGWNKVAHIIELDAQEYEFLVLPE
  AFSIHLPHAPSLDISRFRSSPTYRNCLQALKEEFHQDLSRRYGSAALKYLTALQQARSRA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC096655
• ORF Size: 2072 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC096655, AAH96655
• RefSeq Size: 2398 bp
• RefSeq ORF: 2072 bp
• Locus ID: 228366
• Cytogenetics: 2 E1
• Gene Summary: Bifunctional glycosyltransferase with both xylosyltransferase and beta-1,3-glucuronyltransferase activities involved in the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1). Phosphorylated O-mannosyl trisaccharid is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Elongates the glucuronyl-beta-1,4-xylose-beta disaccharide primer structure by adding repeating units [-3-Xylose-alpha-1,3-GlcA-beta-1-] to produce a heteropolysaccharide. Has a higher activity toward alpha-dystroglycan than LARGE (PubMed:15958417).[UniProtKB/Swiss-Prot Function]