Kifc1 (BC003753) Mouse Tagged ORF Clone

CAT#: MG209950

Kifc1 (tGFP-tagged) - Mouse kinesin family member C1 (cDNA clone MGC:5880 IMAGE:3495308)

Product Images

Circular map for MG209950

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Kifc1
• Synonyms: HSET, KNSL2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209950 representing BC003753
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGTGCAGGCGCAGAGGCCACCTTTGTTGGAAGTGAAGAGGAACGTAGAACTGAAGGCAGCCCTGG
  TGAAGTCCTCCTCCCGAGTGCCCCTGTCAGCAAGCAGGCTCAAGAGGGGTCCTGACCAGATGGAGGATGC
  CTTGGAGCCTGCAAAGAAACGGACACGAGTCATGGGTGCAGTGACCAAAGTTGACACATCCCGTCCCAGA
  GGACCACTCCTCAGCACAGTGTCACAGACCCAGGGCCATACTGCAGCTCAGAAAGGCCCTAAGAAGACAG
  GACCTCGTGGGTGCTCTGCTGTTGGTACAGTGCTGAGGAGCCAGAAGCCAGCTCCCGCTGCTCCTGCCCA
  GAAGCCTGGCACATCCACTGCTCCTGTGGTGGTAGGGAAGAGAGCTGGCAAACGCCCTGCCTGGGACCTG
  AAGGGCCAGTTGTGTGACCTCAATGAAGAGTTGAAACGCTATCGGGAGAAGACTCAAACGCTGGAACTGG
  AGAACCGGGGGCTTCGGGAGCAACTCAGAGAGGTCCAGGAGCAGGCCACGACCCTGGGGACAGAGCGGAA
  CACCCTGGAAGGGGAGCTGGCCAGTGTACGCAGCCGAGCTGAGCAGGACCAGCAGAGGCTGGAGACGCTG
  AGTGCCCGTGTCTTGGAGCTGGAGGAATGTCTGGGTACCAGGGAAAGGCTGCTTCAGGAGCTTCAGGGAG
  AGCGGCTGCAATTGCAGGAAGAGCGGAGCACACTGAGCACCCAGCTGGAGGAGCAGAAGAGGAGGTTTCA
  GGCCACAGAAGCAGCTCTGTCAAGCAGCCAAGAAGAGGTGGTGTGTCTTCGGCAGAAGACTGAAGCCCAG
  GTGACCTTACTGGCTGAGCAAGGAGACCGGCTCTATGGGTTAGAGATGGAGCGGCGGCGACTCCACAACC
  AGCTGCAGGAACTGAAGGGCAATATCCGGGTGTTCTGCCGTGTGCGCCCTGTCCTCGCAGGGGAATCCAC
  TCCATCTCCTGGCTTCCTCGTGTTTCCTCCTGGCCCTGCTGGACCCTCTGATCCCCCGACGGGCCTTAGC
  CTCTCACGATCTGATGATCGGCGCTCCACCCTGACTGGGGCCCCGGCCCCCACTGTCCGCCATGATTTCT
  CCTTTGATCGGGTGTTCCCGCCGGGAAGCAAGCAGGAGGAAGTGTTTGAGGAGATCGCCATGCTTGTCCA
  GTCAGCACTGGATGGCTACCCTGTGTGCATTTTTGCCTATGGACAGACAGGCAGTGGCAAGACCTTCACT
  ATGGAAGGAGGGCCTAGGGGAGACCCCCAATTGGAAGGGCTGATCCCTCGGGCCATGCGGCATCTGTTCT
  CTGTGGCCCAGGAGATGAGCGGCCAGGGCTGGACATACAGTTTTGTGGCGAGTTACGTAGAGATCTACAA
  TGAGACCGTTCGAGACCTGCTAGCTACTGGGCCCCGCAAGGGACAAGGGGGCGAGTGTGAGATCCGTCGG
  GCAAGCCCAGGGAGTGAGGAGCTTACTGTCACCAATGCCCGCTATGTCCCTGTTTCCTGTGAGAAAGAGG
  TGGAGGCCCTGCTCCATTTGGCTCATCAGAACCGGGCTGTGGCCCACACTGCCCAAAATAAGAGATCATC
  ACGCAGTCATAGTGTGTTCCAGCTGCAGATTTCTGGAGAGCATGCAGCTCGGGGCCTGCAGTGTGGCGCT
  CCCCTCAACCTTGTGGACCTAGCTGGGAGTGAGCGGCTAGACCCTGGCTTACACCTAGGCCCTGGGGAGC
  GTGATCGTCTTCGGGAGACACAGGCCATTAACAGCAGTCTGTCTACACTGGGACTGGTCATAATGGCCCT
  GAGCAATAAGGAGTCCCACGTGCCTTACCGAAACAGCAAGCTCACCTACTTGCTGCAGAACTCTCTGGGT
  GGCAGTGCCAAGATGCTTATGTTTGTGAATATTTCTCCTCTGGAAGAGAATGTCTCCGAGTCTCTGAATT
  CACTACGCTTTGCTTCCAAGGTGAACCAGTGTGTCATTGGTACTGCTCAGGCTAATAAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209950 representing BC003753
  Red=Cloning site Green=Tags(s)
  
  MDVQAQRPPLLEVKRNVELKAALVKSSSRVPLSASRLKRGPDQMEDALEPAKKRTRVMGAVTKVDTSRPR
  GPLLSTVSQTQGHTAAQKGPKKTGPRGCSAVGTVLRSQKPAPAAPAQKPGTSTAPVVVGKRAGKRPAWDL
  KGQLCDLNEELKRYREKTQTLELENRGLREQLREVQEQATTLGTERNTLEGELASVRSRAEQDQQRLETL
  SARVLELEECLGTRERLLQELQGERLQLQEERSTLSTQLEEQKRRFQATEAALSSSQEEVVCLRQKTEAQ
  VTLLAEQGDRLYGLEMERRRLHNQLQELKGNIRVFCRVRPVLAGESTPSPGFLVFPPGPAGPSDPPTGLS
  LSRSDDRRSTLTGAPAPTVRHDFSFDRVFPPGSKQEEVFEEIAMLVQSALDGYPVCIFAYGQTGSGKTFT
  MEGGPRGDPQLEGLIPRAMRHLFSVAQEMSGQGWTYSFVASYVEIYNETVRDLLATGPRKGQGGECEIRR
  ASPGSEELTVTNARYVPVSCEKEVEALLHLAHQNRAVAHTAQNKRSSRSHSVFQLQISGEHAARGLQCGA
  PLNLVDLAGSERLDPGLHLGPGERDRLRETQAINSSLSTLGLVIMALSNKESHVPYRNSKLTYLLQNSLG
  GSAKMLMFVNISPLEENVSESLNSLRFASKVNQCVIGTAQANKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC003753
• ORF Size: 2022 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: BC003753, AAH03753
• RefSeq Size: 2263 bp
• RefSeq ORF: 2024 bp
• Locus ID: 16580
• Cytogenetics: 17 A3.3
• Gene Summary: Minus end-directed microtubule-dependent motor required for bipolar spindle formation (PubMed:16638812). May contribute to movement of early endocytic vesicles (PubMed:17360972). Regulates cilium formation and structure (PubMed:23807208).[UniProtKB/Swiss-Prot Function]